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The map label for this gene is purH [H]

Identifier: 225630570

GI number: 225630570

Start: 902372

End: 903883

Strand: Direct

Name: purH [H]

Synonym: WRi_008310

Alternate gene names: 225630570

Gene position: 902372-903883 (Clockwise)

Preceding gene: 225630569

Following gene: 225630571

Centisome position: 62.41

GC content: 34.46

Gene sequence:

>1512_bases
ATGAAAATAAAAAGAGCTTTAATATCAGTATACGATAAAACGAATATAATTGATCTTGCATCGTTTTTAACGCAGCAACA
AATAGAAATTCTTTCAACGGGAAATACTTATAAAACGCTATCTAGTGCAGGAATAAAAACACAAGAGGTCTCAGATTACA
CACAATTTCCAGAGATACTTGGTGGTAGAGTAAAAACTTTACACCCTAAAATTCATGGAGGAATACTTTGCAATAGAGAA
AAACACAAAACGGAAATACAAAATCTAGGTATTAAGCCAATAGACCTGCTTATAACTAACCTATACCCATTTTGGGAGAC
AGTAAATAGCGGCTCAAATGAAGAGCAAATTATAGAACAAATTGATATCGGCGGAGTGGCGTTAATTAGAGCTGCAGCAA
AAAACTTTCGTTTTACTTCAGTTATTTCTAGCATTCAAGACTACGAAGCACTAAAAGCTGAGATGATAAAAAATAACAAT
AAAACAACATTGGAATATAGAAAACACTTAGCAACCAAAGCATTTGCTCTCACTGCACACTACGATTCTAATATTCACAG
TTGGTTTTTATCCCAGAGTAAAAATAATGAGTTGCCAGAGTTTTTTGCTCTATACGGGCATAAAGTACAAGAACTCAGGT
ATGGTGAAAATCCCCATCAAAAAGCTGCATTTTATAGTAATCAATTTACCAAATATCCGTTGGAAAAAATACATGGAAAA
GAGTTGAGTTATAATAATATAGTAGATATAGAATCCGCACTTAACATAACTTCTGAATTCGAAGAACCTGCAGCAGTGAT
AATCAAGCATAATAACCCATGTGGCGCTGCTATTGGTAATAATGCTTTGGAGGCATATGAAAAAGCTCTATCGTGCGATG
AAGTAAGCAGTTTTGGTGGTATAGTTGCCTTAAACCGGGAAATAGATTTAAAGCTAGCGGAAAAATTAAACGAGATATTT
TTAGAAGTTGTGATAGCACCATCAGTAAACAATGAGGCACTAAAAATTTTGCAAAGAAAGAAAAATTTAAGAGTGATTAT
TCATAAATCTTTTCAACAAAATGTGAAATACCAAACTAAAAATGTTATTGGTGGGTTTTTGGTGCAAGAAAATAATGATC
ACAAGATAAAAGCAGAACAAGTAACAGAATGCACTACAACAAAAAAAGGAAAGGAAGATCTTATTTTTGCCTGGAAAATA
TGTAAGCATGTGAAATCCAACGCAATAGTTATAGCAAAAGATGGTTGTGCTATTGGCATCGGCGCAGGGCAAACAAGCAG
AATAGATAGTGTGAACATTGCAGTGAAAAAAGCAGGTGAAAAATGTAAAGGTGCAGTGCTTGCTTCAGATGCATTTTTTC
CATTCCCAGATAGCATAGTAGAAAGTGCAAAACATGGAATTACAGCTATAATTCAGCCCGGCGGCTCGCTGAAAGATCAA
GATGTGATAAAAGCTGCAAATGAAAATAAAATTGCTATGTTTTTCACTGGCGTTCGCAGTTTTTTCCATTAG

Upstream 100 bases:

>100_bases
TAAAAAAATTTATAGAATAGGCTGCTTTAAATTAGTGTAGAAATGTAATAGCTAAATTTCCTTTTCTTAGATATAATCTT
AAAAAACTTTCCATATAAAA

Downstream 100 bases:

>100_bases
GCTTTGTGTCTTATCGTCATACTGCCGCGAACCGTCACGGTATCTCTTGGCCGCTAACAAGCAGCGGAATGACGGCTGTC
GCTTAGCCATAAACATTAAG

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKIKRALISVYDKTNIIDLASFLTQQQIEILSTGNTYKTLSSAGIKTQEVSDYTQFPEILGGRVKTLHPKIHGGILCNRE
KHKTEIQNLGIKPIDLLITNLYPFWETVNSGSNEEQIIEQIDIGGVALIRAAAKNFRFTSVISSIQDYEALKAEMIKNNN
KTTLEYRKHLATKAFALTAHYDSNIHSWFLSQSKNNELPEFFALYGHKVQELRYGENPHQKAAFYSNQFTKYPLEKIHGK
ELSYNNIVDIESALNITSEFEEPAAVIIKHNNPCGAAIGNNALEAYEKALSCDEVSSFGGIVALNREIDLKLAEKLNEIF
LEVVIAPSVNNEALKILQRKKNLRVIIHKSFQQNVKYQTKNVIGGFLVQENNDHKIKAEQVTECTTTKKGKEDLIFAWKI
CKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAVLASDAFFPFPDSIVESAKHGITAIIQPGGSLKDQ
DVIKAANENKIAMFFTGVRSFFH

Sequences:

>Translated_503_residues
MKIKRALISVYDKTNIIDLASFLTQQQIEILSTGNTYKTLSSAGIKTQEVSDYTQFPEILGGRVKTLHPKIHGGILCNRE
KHKTEIQNLGIKPIDLLITNLYPFWETVNSGSNEEQIIEQIDIGGVALIRAAAKNFRFTSVISSIQDYEALKAEMIKNNN
KTTLEYRKHLATKAFALTAHYDSNIHSWFLSQSKNNELPEFFALYGHKVQELRYGENPHQKAAFYSNQFTKYPLEKIHGK
ELSYNNIVDIESALNITSEFEEPAAVIIKHNNPCGAAIGNNALEAYEKALSCDEVSSFGGIVALNREIDLKLAEKLNEIF
LEVVIAPSVNNEALKILQRKKNLRVIIHKSFQQNVKYQTKNVIGGFLVQENNDHKIKAEQVTECTTTKKGKEDLIFAWKI
CKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAVLASDAFFPFPDSIVESAKHGITAIIQPGGSLKDQ
DVIKAANENKIAMFFTGVRSFFH
>Mature_503_residues
MKIKRALISVYDKTNIIDLASFLTQQQIEILSTGNTYKTLSSAGIKTQEVSDYTQFPEILGGRVKTLHPKIHGGILCNRE
KHKTEIQNLGIKPIDLLITNLYPFWETVNSGSNEEQIIEQIDIGGVALIRAAAKNFRFTSVISSIQDYEALKAEMIKNNN
KTTLEYRKHLATKAFALTAHYDSNIHSWFLSQSKNNELPEFFALYGHKVQELRYGENPHQKAAFYSNQFTKYPLEKIHGK
ELSYNNIVDIESALNITSEFEEPAAVIIKHNNPCGAAIGNNALEAYEKALSCDEVSSFGGIVALNREIDLKLAEKLNEIF
LEVVIAPSVNNEALKILQRKKNLRVIIHKSFQQNVKYQTKNVIGGFLVQENNDHKIKAEQVTECTTTKKGKEDLIFAWKI
CKHVKSNAIVIAKDGCAIGIGAGQTSRIDSVNIAVKKAGEKCKGAVLASDAFFPFPDSIVESAKHGITAIIQPGGSLKDQ
DVIKAANENKIAMFFTGVRSFFH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=470, Percent_Identity=36.1702127659575, Blast_Score=249, Evalue=5e-66,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=46.0803059273423, Blast_Score=440, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71985564, Length=473, Percent_Identity=35.5179704016913, Blast_Score=231, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI71985574, Length=308, Percent_Identity=30.8441558441558, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323056, Length=467, Percent_Identity=35.117773019272, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6323768, Length=471, Percent_Identity=34.8195329087049, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24649832, Length=474, Percent_Identity=36.0759493670886, Blast_Score=246, Evalue=3e-65,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55988; Mature: 55988

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRALISVYDKTNIIDLASFLTQQQIEILSTGNTYKTLSSAGIKTQEVSDYTQFPEIL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
GGRVKTLHPKIHGGILCNREKHKTEIQNLGIKPIDLLITNLYPFWETVNSGSNEEQIIEQ
CCCEEEECCCCCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IDIGGVALIRAAAKNFRFTSVISSIQDYEALKAEMIKNNNKTTLEYRKHLATKAFALTAH
HCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEE
YDSNIHSWFLSQSKNNELPEFFALYGHKVQELRYGENPHQKAAFYSNQFTKYPLEKIHGK
CCCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCC
ELSYNNIVDIESALNITSEFEEPAAVIIKHNNPCGAAIGNNALEAYEKALSCDEVSSFGG
CCCCCCEEEHHHHHCHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHCCC
IVALNREIDLKLAEKLNEIFLEVVIAPSVNNEALKILQRKKNLRVIIHKSFQQNVKYQTK
EEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCHHH
NVIGGFLVQENNDHKIKAEQVTECTTTKKGKEDLIFAWKICKHVKSNAIVIAKDGCAIGI
HCCEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEE
GAGQTSRIDSVNIAVKKAGEKCKGAVLASDAFFPFPDSIVESAKHGITAIIQPGGSLKDQ
CCCCCCCCCEEEEEEECCCCHHCCEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCH
DVIKAANENKIAMFFTGVRSFFH
HHHHHCCCCEEEEEEEHHHHHCC
>Mature Secondary Structure
MKIKRALISVYDKTNIIDLASFLTQQQIEILSTGNTYKTLSSAGIKTQEVSDYTQFPEIL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
GGRVKTLHPKIHGGILCNREKHKTEIQNLGIKPIDLLITNLYPFWETVNSGSNEEQIIEQ
CCCEEEECCCCCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IDIGGVALIRAAAKNFRFTSVISSIQDYEALKAEMIKNNNKTTLEYRKHLATKAFALTAH
HCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEE
YDSNIHSWFLSQSKNNELPEFFALYGHKVQELRYGENPHQKAAFYSNQFTKYPLEKIHGK
CCCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCC
ELSYNNIVDIESALNITSEFEEPAAVIIKHNNPCGAAIGNNALEAYEKALSCDEVSSFGG
CCCCCCEEEHHHHHCHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHCCC
IVALNREIDLKLAEKLNEIFLEVVIAPSVNNEALKILQRKKNLRVIIHKSFQQNVKYQTK
EEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCHHH
NVIGGFLVQENNDHKIKAEQVTECTTTKKGKEDLIFAWKICKHVKSNAIVIAKDGCAIGI
HCCEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEE
GAGQTSRIDSVNIAVKKAGEKCKGAVLASDAFFPFPDSIVESAKHGITAIIQPGGSLKDQ
CCCCCCCCCEEEEEEECCCCHHCCEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCH
DVIKAANENKIAMFFTGVRSFFH
HHHHHCCCCEEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]