Click here to switch to the map view.

The map label for this gene is degP [H]

Identifier: 225630545

GI number: 225630545

Start: 859822

End: 861306

Strand: Direct

Name: degP [H]

Synonym: WRi_008000

Alternate gene names: 225630545

Gene position: 859822-861306 (Clockwise)

Preceding gene: 225630544

Following gene: 225630546

Centisome position: 59.47

GC content: 34.41

Gene sequence:

>1485_bases
ATGAAAAGTAAGGCATTTATTTTATCTATATTTGCATATTTTCTAATTGCGTTTTCTTCGTATGCTAATATGTTTGATTG
GAATGCAAAAAAGGTCGTTGATGCTAGCACTACTGCATGCAACTGTAATCAAGGACTTGCCGATTTAGTGGAAGAACTCA
TTCCTGCGGTTGTAAATATTTCAAGCGAACAAATCATCAAACAAGAAAATAACAGCAGAACTAGAGTTCCATCTATGCCA
GGAAATAATTTTTTTGACGATTTTAGAGAGTTTTTTGAGCATTTTGATCAGTTTTTTATGGATAGGGGCCCTAGTGTTAA
CAAAGAGGTGGTGTTGCTTGGTTCTGGGTTTATTATAGATAAAGGTGGAACTATAGTAACCAATTATCACGTTATTAAAA
ACGCCAAAGATATCACAGTTACTATGAACGATAATACTTATTTCAAAGCAGAAGTTTTAGGCTATGATGCAAGAACTGAT
CTTGCTGTGCTTAAGATTAATTCTGATAAAGATCTTTCTTCTGTTGCATTTGGTGATTCTGATAAAGCAAGGGTTGGTGA
TACGGTTATGGCAATAGGTAACCCATTTGGTTTGGGTGGCTCTGTAAGCACAGGAATTATATCTGCAAGGTCCAGGGACA
TTAGTATTGGCACTATGAATGAATTTATTCAAACTGATGCTGCAATTAATAGGGGTAACTCTGGAGGACCGTTATTTGAT
TTAAATGGAAAAGTTATAGGTATTAATACTGCTATCTATTCTCCATCTGAGTCTGGCGGCAACGTGGGTATAGGTTTTGC
CATACCATCTAATTTAGCTATGTCAATTATTGACACATTAAAAAGTGGCAAAAAAATAAAACATGGTTGGCTTGGTGTGC
AAGTTCAGCCTATAACGAAAGAATTTGCTGAGTCCTTGGGTTTAAAAGATATAAAAGGCGCATTGGTTGCAAGTATAGTA
AAGGATAGTCCTGCAGAAAAAGGTGGGATTAAAGTAGGTGATATATTATTAGAATTTGACGGTAAAAAAATCGATAGAAT
GACACAATTACCTCAAATGGTTTCAAGAGCTGGACCTGAGAAAAAAGTGCAAGTTAAGTTACTTAGAAAGAGCAAAGAGG
TTAATATTAAAGTTGTGATCGGGGAATCTACAAATGATGGCCAAGATAACAATCAAGAAGAAAATAAATCAACATCTGAT
TATGTAACCGGTTTAACTGTTTCAAATCTGCCAAAAGAATCAAAAGAAAGTAATCCTACAAAAGGTGTGATAGTTACCAA
TGTAGATAGTAACAGTAATGCCACGTTGCGTGGTATTAAAAAAGGAGACATTATTATCCAATTAGATGGAACCGATATAG
AAAATACTAATGATTTTCAAAAACAAGTTGATTCAGCAGTAAAGAAAAACGGTAAAGATTCAATAATGTTGCTCATTTAC
CGCAATGGAAATCAATTCTTTACTTCGATAAAGTTGAAGAAGTAA

Upstream 100 bases:

>100_bases
GAGCGCTTACAGTAAATCATTTGCCGAAAATAATACTAAATTTGTACTTTCACCAAACAATAACTTTTTAGATATTTTAA
ATAAAGGATGGAAATAATTT

Downstream 100 bases:

>100_bases
TTTTATTCACTAAATTTACAAAACAGTAGTGTTGTAAGCTCGCTACTGTAACACTTCAATCTTATAAAATAAATAAAAAT
CTACACTAGATTTCTTGCAT

Product: Trypsin-like serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKSKAFILSIFAYFLIAFSSYANMFDWNAKKVVDASTTACNCNQGLADLVEELIPAVVNISSEQIIKQENNSRTRVPSMP
GNNFFDDFREFFEHFDQFFMDRGPSVNKEVVLLGSGFIIDKGGTIVTNYHVIKNAKDITVTMNDNTYFKAEVLGYDARTD
LAVLKINSDKDLSSVAFGDSDKARVGDTVMAIGNPFGLGGSVSTGIISARSRDISIGTMNEFIQTDAAINRGNSGGPLFD
LNGKVIGINTAIYSPSESGGNVGIGFAIPSNLAMSIIDTLKSGKKIKHGWLGVQVQPITKEFAESLGLKDIKGALVASIV
KDSPAEKGGIKVGDILLEFDGKKIDRMTQLPQMVSRAGPEKKVQVKLLRKSKEVNIKVVIGESTNDGQDNNQEENKSTSD
YVTGLTVSNLPKESKESNPTKGVIVTNVDSNSNATLRGIKKGDIIIQLDGTDIENTNDFQKQVDSAVKKNGKDSIMLLIY
RNGNQFFTSIKLKK

Sequences:

>Translated_494_residues
MKSKAFILSIFAYFLIAFSSYANMFDWNAKKVVDASTTACNCNQGLADLVEELIPAVVNISSEQIIKQENNSRTRVPSMP
GNNFFDDFREFFEHFDQFFMDRGPSVNKEVVLLGSGFIIDKGGTIVTNYHVIKNAKDITVTMNDNTYFKAEVLGYDARTD
LAVLKINSDKDLSSVAFGDSDKARVGDTVMAIGNPFGLGGSVSTGIISARSRDISIGTMNEFIQTDAAINRGNSGGPLFD
LNGKVIGINTAIYSPSESGGNVGIGFAIPSNLAMSIIDTLKSGKKIKHGWLGVQVQPITKEFAESLGLKDIKGALVASIV
KDSPAEKGGIKVGDILLEFDGKKIDRMTQLPQMVSRAGPEKKVQVKLLRKSKEVNIKVVIGESTNDGQDNNQEENKSTSD
YVTGLTVSNLPKESKESNPTKGVIVTNVDSNSNATLRGIKKGDIIIQLDGTDIENTNDFQKQVDSAVKKNGKDSIMLLIY
RNGNQFFTSIKLKK
>Mature_494_residues
MKSKAFILSIFAYFLIAFSSYANMFDWNAKKVVDASTTACNCNQGLADLVEELIPAVVNISSEQIIKQENNSRTRVPSMP
GNNFFDDFREFFEHFDQFFMDRGPSVNKEVVLLGSGFIIDKGGTIVTNYHVIKNAKDITVTMNDNTYFKAEVLGYDARTD
LAVLKINSDKDLSSVAFGDSDKARVGDTVMAIGNPFGLGGSVSTGIISARSRDISIGTMNEFIQTDAAINRGNSGGPLFD
LNGKVIGINTAIYSPSESGGNVGIGFAIPSNLAMSIIDTLKSGKKIKHGWLGVQVQPITKEFAESLGLKDIKGALVASIV
KDSPAEKGGIKVGDILLEFDGKKIDRMTQLPQMVSRAGPEKKVQVKLLRKSKEVNIKVVIGESTNDGQDNNQEENKSTSD
YVTGLTVSNLPKESKESNPTKGVIVTNVDSNSNATLRGIKKGDIIIQLDGTDIENTNDFQKQVDSAVKKNGKDSIMLLIY
RNGNQFFTSIKLKK

Specific function: Serine Protease That Is Required At High Temperature. Involved In The Degradation Of Damaged Proteins. It Can Degrade Icia, Ada, Casein And Globin. Shared Specificity With Degq. [C]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Homo sapiens, GI4506141, Length=353, Percent_Identity=31.4447592067989, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI22129776, Length=300, Percent_Identity=32.6666666666667, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI7019477, Length=259, Percent_Identity=33.2046332046332, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI24308541, Length=250, Percent_Identity=34, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786356, Length=468, Percent_Identity=35.4700854700855, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1789629, Length=443, Percent_Identity=34.3115124153499, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789630, Length=276, Percent_Identity=37.6811594202899, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24646839, Length=309, Percent_Identity=30.42071197411, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.-

Molecular weight: Translated: 53647; Mature: 53647

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKAFILSIFAYFLIAFSSYANMFDWNAKKVVDASTTACNCNQGLADLVEELIPAVVNI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
SSEQIIKQENNSRTRVPSMPGNNFFDDFREFFEHFDQFFMDRGPSVNKEVVLLGSGFIID
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEE
KGGTIVTNYHVIKNAKDITVTMNDNTYFKAEVLGYDARTDLAVLKINSDKDLSSVAFGDS
CCCEEEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEECCCC
DKARVGDTVMAIGNPFGLGGSVSTGIISARSRDISIGTMNEFIQTDAAINRGNSGGPLFD
CCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHHCCCCCCCCEEE
LNGKVIGINTAIYSPSESGGNVGIGFAIPSNLAMSIIDTLKSGKKIKHGWLGVQVQPITK
CCCEEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCEEEEEEEHHHH
EFAESLGLKDIKGALVASIVKDSPAEKGGIKVGDILLEFDGKKIDRMTQLPQMVSRAGPE
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCC
KKVQVKLLRKSKEVNIKVVIGESTNDGQDNNQEENKSTSDYVTGLTVSNLPKESKESNPT
CCEEHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEHHCCCCCCCCCCCC
KGVIVTNVDSNSNATLRGIKKGDIIIQLDGTDIENTNDFQKQVDSAVKKNGKDSIMLLIY
CCEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
RNGNQFFTSIKLKK
ECCCEEEEEEEECC
>Mature Secondary Structure
MKSKAFILSIFAYFLIAFSSYANMFDWNAKKVVDASTTACNCNQGLADLVEELIPAVVNI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
SSEQIIKQENNSRTRVPSMPGNNFFDDFREFFEHFDQFFMDRGPSVNKEVVLLGSGFIID
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEE
KGGTIVTNYHVIKNAKDITVTMNDNTYFKAEVLGYDARTDLAVLKINSDKDLSSVAFGDS
CCCEEEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCEEEEEECCCCCCCCEECCCC
DKARVGDTVMAIGNPFGLGGSVSTGIISARSRDISIGTMNEFIQTDAAINRGNSGGPLFD
CCCCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEECHHHHHHHHHHHHCCCCCCCCEEE
LNGKVIGINTAIYSPSESGGNVGIGFAIPSNLAMSIIDTLKSGKKIKHGWLGVQVQPITK
CCCEEEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCEEEEEEEHHHH
EFAESLGLKDIKGALVASIVKDSPAEKGGIKVGDILLEFDGKKIDRMTQLPQMVSRAGPE
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCC
KKVQVKLLRKSKEVNIKVVIGESTNDGQDNNQEENKSTSDYVTGLTVSNLPKESKESNPT
CCEEHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEEEEHHCCCCCCCCCCCC
KGVIVTNVDSNSNATLRGIKKGDIIIQLDGTDIENTNDFQKQVDSAVKKNGKDSIMLLIY
CCEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
RNGNQFFTSIKLKK
ECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7861951 [H]