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The map label for this gene is ispB [H]

Identifier: 225630516

GI number: 225630516

Start: 830985

End: 831962

Strand: Direct

Name: ispB [H]

Synonym: WRi_007680

Alternate gene names: 225630516

Gene position: 830985-831962 (Clockwise)

Preceding gene: 225630515

Following gene: 225630517

Centisome position: 57.47

GC content: 34.76

Gene sequence:

>978_bases
ATGCTAATAACAAACAGCGATAAATTAGACGATATTGTATCTTCTGATTTGTCAGCTATGAATGACTTTGTCTTCAAAAA
TGTCAATGATGAAGATGCTAAGCTTGCTACTGATATTATATCTCATCTTGTTAGGTCAGGTGGAAAAAAAATAAGGCCTA
AACTAGTTTTTATTATATGTAAAATGCTAAACTATTCTGGGGAGAATAGGATCAACGTTGCTGCATCGGTAGAGTTTATA
CACAATGCTACTTTACTTCATGATGATGTGCTCGATGAAAGTGAGGCACGCCATGGAGTTAAAACAGCGAATAAGATTTG
GGGAAATAAATCAAGCATTTTAGTTGGTGATCTATTGTTGACTCTAGCATTTAGATGGCTTATAGAGTGTGGAAATTTAA
ATATTCTCTCTATTTTATCTAAAGCATCGCATTTACTTGTGAAGGGTGAAATAAAGCAGATGACAGCACGCTTTGACCCC
CACACAGTCAGGGAAAATTATTTTGATATCATTGGAGAAAAGACAGCATCTTTATTTTCTGCATGCTGTGAAGCTGCATC
AACAGTATCTGGTGCAACAAATGACGAAACAGAGAGGCTAAAGAATTTTGGTTTTAATTTTGGTATGGCATTTCAAATAG
TTGATGATATGCTCGATTATACTGCTAATCAAGGCACTTCTGGAAAGCAAGTCGGAAAAGACTTTTTCGAAGGTAAAGTC
ACCTTACCTGCTATTATAGCATACGAAAAGGGCAGTCCAGCAGAGCAAAAATTCTGGGAAAAATGCTTTTCTTCAGCTAG
ACACAATTTTGACCAAGCATTACACTATATTAATCACCATAATGCCATTCAGCTTTCTATGGAAAAAGCTAGACACTACA
TTAATATGGCACAAAATGATATTAGTACTTTTTCTGATTCTTTTTATAAAACTACTTTAATTAACTTTTTAAATGCGAGC
ATAGAAAGACGAATGTAG

Upstream 100 bases:

>100_bases
AAAAAAGGCATAGTAACTTTAGAAACAGAACCAAAGAAAACAGAAAAGAAGAAAGTAGAGAACGAGAAAGCACAAGGGCA
AGAGGCATAAGTCTCTTAAA

Downstream 100 bases:

>100_bases
CTGGCGCTTGATATTTTCAGATTAATTGTTATATATTTATTAGATTCTTAAGGGTAATGATATGTCAGATAGCAGTAAGG
AGAAAAAGAAGAAGTTTGCT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLITNSDKLDDIVSSDLSAMNDFVFKNVNDEDAKLATDIISHLVRSGGKKIRPKLVFIICKMLNYSGENRINVAASVEFI
HNATLLHDDVLDESEARHGVKTANKIWGNKSSILVGDLLLTLAFRWLIECGNLNILSILSKASHLLVKGEIKQMTARFDP
HTVRENYFDIIGEKTASLFSACCEAASTVSGATNDETERLKNFGFNFGMAFQIVDDMLDYTANQGTSGKQVGKDFFEGKV
TLPAIIAYEKGSPAEQKFWEKCFSSARHNFDQALHYINHHNAIQLSMEKARHYINMAQNDISTFSDSFYKTTLINFLNAS
IERRM

Sequences:

>Translated_325_residues
MLITNSDKLDDIVSSDLSAMNDFVFKNVNDEDAKLATDIISHLVRSGGKKIRPKLVFIICKMLNYSGENRINVAASVEFI
HNATLLHDDVLDESEARHGVKTANKIWGNKSSILVGDLLLTLAFRWLIECGNLNILSILSKASHLLVKGEIKQMTARFDP
HTVRENYFDIIGEKTASLFSACCEAASTVSGATNDETERLKNFGFNFGMAFQIVDDMLDYTANQGTSGKQVGKDFFEGKV
TLPAIIAYEKGSPAEQKFWEKCFSSARHNFDQALHYINHHNAIQLSMEKARHYINMAQNDISTFSDSFYKTTLINFLNAS
IERRM
>Mature_325_residues
MLITNSDKLDDIVSSDLSAMNDFVFKNVNDEDAKLATDIISHLVRSGGKKIRPKLVFIICKMLNYSGENRINVAASVEFI
HNATLLHDDVLDESEARHGVKTANKIWGNKSSILVGDLLLTLAFRWLIECGNLNILSILSKASHLLVKGEIKQMTARFDP
HTVRENYFDIIGEKTASLFSACCEAASTVSGATNDETERLKNFGFNFGMAFQIVDDMLDYTANQGTSGKQVGKDFFEGKV
TLPAIIAYEKGSPAEQKFWEKCFSSARHNFDQALHYINHHNAIQLSMEKARHYINMAQNDISTFSDSFYKTTLINFLNAS
IERRM

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=276, Percent_Identity=32.2463768115942, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786623, Length=244, Percent_Identity=29.0983606557377, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=187, Percent_Identity=36.8983957219251, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319475, Length=297, Percent_Identity=29.2929292929293, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325188, Length=248, Percent_Identity=25.4032258064516, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24651612, Length=309, Percent_Identity=26.8608414239482, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281365769, Length=224, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24660002, Length=224, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36467; Mature: 36467

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITNSDKLDDIVSSDLSAMNDFVFKNVNDEDAKLATDIISHLVRSGGKKIRPKLVFIIC
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KMLNYSGENRINVAASVEFIHNATLLHDDVLDESEARHGVKTANKIWGNKSSILVGDLLL
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLAFRWLIECGNLNILSILSKASHLLVKGEIKQMTARFDPHTVRENYFDIIGEKTASLFS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ACCEAASTVSGATNDETERLKNFGFNFGMAFQIVDDMLDYTANQGTSGKQVGKDFFEGKV
HHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
TLPAIIAYEKGSPAEQKFWEKCFSSARHNFDQALHYINHHNAIQLSMEKARHYINMAQND
CCCEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
ISTFSDSFYKTTLINFLNASIERRM
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLITNSDKLDDIVSSDLSAMNDFVFKNVNDEDAKLATDIISHLVRSGGKKIRPKLVFIIC
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KMLNYSGENRINVAASVEFIHNATLLHDDVLDESEARHGVKTANKIWGNKSSILVGDLLL
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLAFRWLIECGNLNILSILSKASHLLVKGEIKQMTARFDPHTVRENYFDIIGEKTASLFS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ACCEAASTVSGATNDETERLKNFGFNFGMAFQIVDDMLDYTANQGTSGKQVGKDFFEGKV
HHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
TLPAIIAYEKGSPAEQKFWEKCFSSARHNFDQALHYINHHNAIQLSMEKARHYINMAQND
CCCEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
ISTFSDSFYKTTLINFLNASIERRM
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]