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The map label for this gene is grpE [H]

Identifier: 225630517

GI number: 225630517

Start: 832024

End: 832593

Strand: Direct

Name: grpE [H]

Synonym: WRi_007690

Alternate gene names: 225630517

Gene position: 832024-832593 (Clockwise)

Preceding gene: 225630516

Following gene: 225630518

Centisome position: 57.54

GC content: 36.67

Gene sequence:

>570_bases
ATGTCAGATAGCAGTAAGGAGAAAAAGAAGAAGTTTGCTGATATGGTAAGTAGGCAGAAAGGTGACGACCAACAAAGTGA
TAACCATAAGCAAACTGATGATTTAAATGAAGATCTCAATACATTAAAAGAACGTGCAGTTCAGCTTGAAGATCATTTAC
GCCGTGCTGTTGCAGATAATGAAAACGTCAAACGTATAATGCAAAAGCAAATTAGCGATGCAAGTGACTACGCAGTCACA
AAACTTGCACGTGATATGATCGACTCGTGCGACAATTTAAAAAGAGTAATGGAAATTTTGAAAGACGGTGATCCTGTTCA
TGAGGGGATTAAAGTAGCTTATCAAAAAATTATAAATGATCTAAAAAAACATGGAATAGAGGAAGTAGATCCACTTGGTG
AGCTTTTTGATAGTAATTTACACCAAGCTGTTGTGGAAAGAGAAGACAATGAAAAAAAGCCTGGCACCATTGTAGAAGTA
CTACAAACTGGTTATACTATCAAAAATAGGTTGCTCCGTCCTGCAATGGTTATTCTTTCTAAGAAATCTGCTGATTGTGG
AAGCGATTAG

Upstream 100 bases:

>100_bases
ATTAACTTTTTAAATGCGAGCATAGAAAGACGAATGTAGCTGGCGCTTGATATTTTCAGATTAATTGTTATATATTTATT
AGATTCTTAAGGGTAATGAT

Downstream 100 bases:

>100_bases
CATGAGGTAAATGTATAATGACAAGTATTGTCTTTTCAGGTATCCAGCCAAGTGGCGTACTACATTTAGGTAATTATCTT
GGTGCAATCAAACAGTGGAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSDSSKEKKKKFADMVSRQKGDDQQSDNHKQTDDLNEDLNTLKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVT
KLARDMIDSCDNLKRVMEILKDGDPVHEGIKVAYQKIINDLKKHGIEEVDPLGELFDSNLHQAVVEREDNEKKPGTIVEV
LQTGYTIKNRLLRPAMVILSKKSADCGSD

Sequences:

>Translated_189_residues
MSDSSKEKKKKFADMVSRQKGDDQQSDNHKQTDDLNEDLNTLKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVT
KLARDMIDSCDNLKRVMEILKDGDPVHEGIKVAYQKIINDLKKHGIEEVDPLGELFDSNLHQAVVEREDNEKKPGTIVEV
LQTGYTIKNRLLRPAMVILSKKSADCGSD
>Mature_188_residues
SDSSKEKKKKFADMVSRQKGDDQQSDNHKQTDDLNEDLNTLKERAVQLEDHLRRAVADNENVKRIMQKQISDASDYAVTK
LARDMIDSCDNLKRVMEILKDGDPVHEGIKVAYQKIINDLKKHGIEEVDPLGELFDSNLHQAVVEREDNEKKPGTIVEVL
QTGYTIKNRLLRPAMVILSKKSADCGSD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=159, Percent_Identity=30.8176100628931, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI40255109, Length=193, Percent_Identity=26.9430051813472, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788967, Length=138, Percent_Identity=28.9855072463768, Blast_Score=77, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17552458, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=155, Percent_Identity=36.7741935483871, Blast_Score=114, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24653432, Length=168, Percent_Identity=29.7619047619048, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21461; Mature: 21330

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSKEKKKKFADMVSRQKGDDQQSDNHKQTDDLNEDLNTLKERAVQLEDHLRRAVADN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ENVKRIMQKQISDASDYAVTKLARDMIDSCDNLKRVMEILKDGDPVHEGIKVAYQKIIND
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LKKHGIEEVDPLGELFDSNLHQAVVEREDNEKKPGTIVEVLQTGYTIKNRLLRPAMVILS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSADCGSD
CCCCCCCCC
>Mature Secondary Structure 
SDSSKEKKKKFADMVSRQKGDDQQSDNHKQTDDLNEDLNTLKERAVQLEDHLRRAVADN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ENVKRIMQKQISDASDYAVTKLARDMIDSCDNLKRVMEILKDGDPVHEGIKVAYQKIIND
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LKKHGIEEVDPLGELFDSNLHQAVVEREDNEKKPGTIVEVLQTGYTIKNRLLRPAMVILS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSADCGSD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA