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The map label for this gene is cisA [H]

Identifier: 225630504

GI number: 225630504

Start: 817173

End: 818804

Strand: Reverse

Name: cisA [H]

Synonym: WRi_007550

Alternate gene names: 225630504

Gene position: 818804-817173 (Counterclockwise)

Preceding gene: 225630507

Following gene: 225630503

Centisome position: 56.63

GC content: 34.38

Gene sequence:

>1632_bases
ATGGTAACGGTAAGCTTGTATGCAAGAGTTTCATCAAGACAACAGGCACAGGAGAATACAATAGAGAGTCAGATTGTAGA
ACTGGAGCGTCGCATTAGTAGTGATGGACATGAGTTATTAGATGAGCACAGGTTTGTCGATAATGGTTATAGTGGATCAA
ATTTAGAACGTCCTGGCTTAGAGAGTTTACGTGATAGAGTAGCAGAAGGTAAGATTGATAAGATATATATTCATTCACCT
GATCGGCTATCACGTAAATTTTCATATCAAATGATCTTACTCGAAGAGTTTAAAAAGGCTGGATCTGAAATAGTATTTTT
GAATCATAAGTTTGATGATAATCCTGATTCCCATTTATTTCTACAGATTCAGGGAGCAATAGCAGAGTATGAACGTGCAA
AGATTATGGAACGAAACCGTAGAGGTAAACTTCATGCGGCCAAAGCTGGGTGTATAAGTGTGATGGGTAGGGCGCCTTAC
GGTTATCGCTACATAGCAAAACATGTAGGTGAAGGAAGTGCTCAATTTGAGGTTGATGAAGAAGAGGCAAATATAGTGCG
TAAAATATTTAGTCGGGTTGGCCAAGAAAGAGCAAGTATAGGAGAAGTTGTACATGAACTGAATAAGATACCGATAATAA
CACGAACAGGAAAAAGGTATTGGAAAAGGAGCACTATTTGGAACATGTTAAAAAATCCTGCTTATATAGGACAAGCAGCT
TATGGTAAAACTAGGACATGCTCAAAACCACAAGTAAAAAAATCAAAAAAAGGAACTTGTGGTAAACTTAAAAGTGGTCG
CTTTAATAGTGATAAAGAGAATTGGACTTATATTCCAGTACCAAAAATAATCAATGAAAATTTATTTGATTCAGTGCAAA
CACAATTAGCTGAAAATAGACAAAGAGCAAGAGTACGGCAAAGAAGAGAAACATATTTATTACAAGGTTTAATGGTATGC
CAACGTTGTCAATATACTTATTGTGGTACAAACCACGTTCATAAAAAGTCGACATATTATTACTATCGTTGCTCTGGTAC
AAACTCTAGCAAATTTAATGGCAATAAAATCTGCGATAATAAATCAATACGCACTGATGTACTCGATACAGTCGTATGGG
AGGAAGTTAAGAGTATATTAAAAGAGCCAGATAGGATTGCAAATGAATATCAACGTAGATTGTCAGAGAACAAAAAACCT
TTACATAATCAAACACGTGAAAAGCAAGAAAGTAAGTTAAGATTAAGTATCAAAAAATTTATCGATAGCTATGCCAAAGG
GTTTATAAGCCAAGAAGAATTTGAGCCAAGAATTACAACAATGAAACAGCATTTGAAAGAAATTGAGGAGGAAAAAGAAA
AAGTACTTGATCAAAAGAAATTACAACAAGAATTGAGCCTTGTTACAGATAGCTTGAAAAACTTTTCCTCAAGTGTTGAA
TCAAAGCTTGATCAAGTAGATTGGCAGACTAAGCAAAATATTATTAGAATGTTAATTCATCAAATTGAAATCAACCACAA
CCACTTATACATAGTATTTCGCATAAAAAGTCTTGCAAATTTTGATCAAAATAGGCATAATAGAATTATGCAATGTTGTA
CTAGCAGTCAATATTGTTGGATTGCCAGATAA

Upstream 100 bases:

>100_bases
ACTATTAATTGCCGTTATGAACCTCACAGATTGGCGAGTACATATTTGGCAAATGCTTATGAACAATTATTGAAATATAA
AATAAAGGAGGTAGAAGAAA

Downstream 100 bases:

>100_bases
AACTGTTGCGGAATCCAAAGAACGCATCAGAGCAGCGTTAAATTCAATCAATCTACTACTACCTCCAAAAAGAATTACAG
TTAATCTCTCCCCTGCGGAT

Product: Putative cassette chromosome recombinase resolvase, CcrB-like protein

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MVTVSLYARVSSRQQAQENTIESQIVELERRISSDGHELLDEHRFVDNGYSGSNLERPGLESLRDRVAEGKIDKIYIHSP
DRLSRKFSYQMILLEEFKKAGSEIVFLNHKFDDNPDSHLFLQIQGAIAEYERAKIMERNRRGKLHAAKAGCISVMGRAPY
GYRYIAKHVGEGSAQFEVDEEEANIVRKIFSRVGQERASIGEVVHELNKIPIITRTGKRYWKRSTIWNMLKNPAYIGQAA
YGKTRTCSKPQVKKSKKGTCGKLKSGRFNSDKENWTYIPVPKIINENLFDSVQTQLAENRQRARVRQRRETYLLQGLMVC
QRCQYTYCGTNHVHKKSTYYYYRCSGTNSSKFNGNKICDNKSIRTDVLDTVVWEEVKSILKEPDRIANEYQRRLSENKKP
LHNQTREKQESKLRLSIKKFIDSYAKGFISQEEFEPRITTMKQHLKEIEEEKEKVLDQKKLQQELSLVTDSLKNFSSSVE
SKLDQVDWQTKQNIIRMLIHQIEINHNHLYIVFRIKSLANFDQNRHNRIMQCCTSSQYCWIAR

Sequences:

>Translated_543_residues
MVTVSLYARVSSRQQAQENTIESQIVELERRISSDGHELLDEHRFVDNGYSGSNLERPGLESLRDRVAEGKIDKIYIHSP
DRLSRKFSYQMILLEEFKKAGSEIVFLNHKFDDNPDSHLFLQIQGAIAEYERAKIMERNRRGKLHAAKAGCISVMGRAPY
GYRYIAKHVGEGSAQFEVDEEEANIVRKIFSRVGQERASIGEVVHELNKIPIITRTGKRYWKRSTIWNMLKNPAYIGQAA
YGKTRTCSKPQVKKSKKGTCGKLKSGRFNSDKENWTYIPVPKIINENLFDSVQTQLAENRQRARVRQRRETYLLQGLMVC
QRCQYTYCGTNHVHKKSTYYYYRCSGTNSSKFNGNKICDNKSIRTDVLDTVVWEEVKSILKEPDRIANEYQRRLSENKKP
LHNQTREKQESKLRLSIKKFIDSYAKGFISQEEFEPRITTMKQHLKEIEEEKEKVLDQKKLQQELSLVTDSLKNFSSSVE
SKLDQVDWQTKQNIIRMLIHQIEINHNHLYIVFRIKSLANFDQNRHNRIMQCCTSSQYCWIAR
>Mature_543_residues
MVTVSLYARVSSRQQAQENTIESQIVELERRISSDGHELLDEHRFVDNGYSGSNLERPGLESLRDRVAEGKIDKIYIHSP
DRLSRKFSYQMILLEEFKKAGSEIVFLNHKFDDNPDSHLFLQIQGAIAEYERAKIMERNRRGKLHAAKAGCISVMGRAPY
GYRYIAKHVGEGSAQFEVDEEEANIVRKIFSRVGQERASIGEVVHELNKIPIITRTGKRYWKRSTIWNMLKNPAYIGQAA
YGKTRTCSKPQVKKSKKGTCGKLKSGRFNSDKENWTYIPVPKIINENLFDSVQTQLAENRQRARVRQRRETYLLQGLMVC
QRCQYTYCGTNHVHKKSTYYYYRCSGTNSSKFNGNKICDNKSIRTDVLDTVVWEEVKSILKEPDRIANEYQRRLSENKKP
LHNQTREKQESKLRLSIKKFIDSYAKGFISQEEFEPRITTMKQHLKEIEEEKEKVLDQKKLQQELSLVTDSLKNFSSSVE
SKLDQVDWQTKQNIIRMLIHQIEINHNHLYIVFRIKSLANFDQNRHNRIMQCCTSSQYCWIAR

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 63486; Mature: 63486

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVSLYARVSSRQQAQENTIESQIVELERRISSDGHELLDEHRFVDNGYSGSNLERPGL
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCH
ESLRDRVAEGKIDKIYIHSPDRLSRKFSYQMILLEEFKKAGSEIVFLNHKFDDNPDSHLF
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEE
LQIQGAIAEYERAKIMERNRRGKLHAAKAGCISVMGRAPYGYRYIAKHVGEGSAQFEVDE
EEEECHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECH
EEANIVRKIFSRVGQERASIGEVVHELNKIPIITRTGKRYWKRSTIWNMLKNPAYIGQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHCHHC
YGKTRTCSKPQVKKSKKGTCGKLKSGRFNSDKENWTYIPVPKIINENLFDSVQTQLAENR
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
QRARVRQRRETYLLQGLMVCQRCQYTYCGTNHVHKKSTYYYYRCSGTNSSKFNGNKICDN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
KSIRTDVLDTVVWEEVKSILKEPDRIANEYQRRLSENKKPLHNQTREKQESKLRLSIKKF
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IDSYAKGFISQEEFEPRITTMKQHLKEIEEEKEKVLDQKKLQQELSLVTDSLKNFSSSVE
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLDQVDWQTKQNIIRMLIHQIEINHNHLYIVFRIKSLANFDQNRHNRIMQCCTSSQYCW
HHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCEE
IAR
EEC
>Mature Secondary Structure
MVTVSLYARVSSRQQAQENTIESQIVELERRISSDGHELLDEHRFVDNGYSGSNLERPGL
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCH
ESLRDRVAEGKIDKIYIHSPDRLSRKFSYQMILLEEFKKAGSEIVFLNHKFDDNPDSHLF
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEE
LQIQGAIAEYERAKIMERNRRGKLHAAKAGCISVMGRAPYGYRYIAKHVGEGSAQFEVDE
EEEECHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECH
EEANIVRKIFSRVGQERASIGEVVHELNKIPIITRTGKRYWKRSTIWNMLKNPAYIGQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCHHHCHHC
YGKTRTCSKPQVKKSKKGTCGKLKSGRFNSDKENWTYIPVPKIINENLFDSVQTQLAENR
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
QRARVRQRRETYLLQGLMVCQRCQYTYCGTNHVHKKSTYYYYRCSGTNSSKFNGNKICDN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
KSIRTDVLDTVVWEEVKSILKEPDRIANEYQRRLSENKKPLHNQTREKQESKLRLSIKKF
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IDSYAKGFISQEEFEPRITTMKQHLKEIEEEKEKVLDQKKLQQELSLVTDSLKNFSSSVE
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLDQVDWQTKQNIIRMLIHQIEINHNHLYIVFRIKSLANFDQNRHNRIMQCCTSSQYCW
HHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCEE
IAR
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]