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The map label for this gene is nth [H]

Identifier: 225630400

GI number: 225630400

Start: 681124

End: 681762

Strand: Reverse

Name: nth [H]

Synonym: WRi_006310

Alternate gene names: 225630400

Gene position: 681762-681124 (Counterclockwise)

Preceding gene: 225630401

Following gene: 225630399

Centisome position: 47.15

GC content: 35.84

Gene sequence:

>639_bases
ATGGACTCAAAAAAAGTAGAATTGATATTTGAAAAGTTTCAACAATCAAATCCTGCACCAAAAATAGAGCTGAATTATAC
CAATGATTTTACGTTATTAGTTGCGATAGTTCTATCAGCGCGAACAACTGATATAAGCGTTAATAAAATTACAAAGGAGC
TATTTAGTATCACTGATACGCCAGAAAAAATGCTGAGTTTTGGGCAGAGTGAGCTAAGAAAATGCATAAGCAGTATTGGT
TTATATAATTCCAAAGCAAAAAACATAATCGGGCTCAGCAAAATATTGATTGAGAGGTACAATAGCAAAGTGCCTACTGA
TTTCGATGATTTGGTGTCTTTGCCGGGAGTGGGAAGAAAGAGCGCTAATGTGTTCTTAAATTCGGGGCTTGGCATTCCAA
CATTGGCAGTTGATACTCACGTTTTTAGAGTGAGCAATAGAATCGGGCTTGTGAAAGAAAAAGATGTGTTTAAAACAGAA
CAATCTCTTTTAAATGTGGTTCCCAAAAAATACCTGCTTTATGCTCATCATTGGCTAGTTTTGCATGGTAGATATGTTTG
CAAAGCACAGAAACCTTCGTGTGAAACGTGCATAATTCATGATTTATGTGAATTTGAGTGCAAAAGGTATAAAGTCTAG

Upstream 100 bases:

>100_bases
AAAAACTGATATGTCTGATGTTAATGCTAAAGGACTATCTAAGCCTAAAGGAATTGAATTTGCACAGCAACCAGTGTAGT
ATAAGCTTTTAGCTCTATGC

Downstream 100 bases:

>100_bases
TATTTCTTTTTTTGTCATCCAAGTAGCTATAGAAACGAAAAAACTTACTTGACACATTTTGCTGGAATGATACAAGTTAT
TATTCAAACAACAGTTATGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNDFTLLVAIVLSARTTDISVNKITKELFSITDTPEKMLSFGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTDFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRIGLVKEKDVFKTE
QSLLNVVPKKYLLYAHHWLVLHGRYVCKAQKPSCETCIIHDLCEFECKRYKV

Sequences:

>Translated_212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNDFTLLVAIVLSARTTDISVNKITKELFSITDTPEKMLSFGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTDFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRIGLVKEKDVFKTE
QSLLNVVPKKYLLYAHHWLVLHGRYVCKAQKPSCETCIIHDLCEFECKRYKV
>Mature_212_residues
MDSKKVELIFEKFQQSNPAPKIELNYTNDFTLLVAIVLSARTTDISVNKITKELFSITDTPEKMLSFGQSELRKCISSIG
LYNSKAKNIIGLSKILIERYNSKVPTDFDDLVSLPGVGRKSANVFLNSGLGIPTLAVDTHVFRVSNRIGLVKEKDVFKTE
QSLLNVVPKKYLLYAHHWLVLHGRYVCKAQKPSCETCIIHDLCEFECKRYKV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=50, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=35.195530726257, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.8421052631579, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=31.0344827586207, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=32.7777777777778, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24001; Mature: 24001

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSKKVELIFEKFQQSNPAPKIELNYTNDFTLLVAIVLSARTTDISVNKITKELFSITDT
CCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PEKMLSFGQSELRKCISSIGLYNSKAKNIIGLSKILIERYNSKVPTDFDDLVSLPGVGRK
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
SANVFLNSGLGIPTLAVDTHVFRVSNRIGLVKEKDVFKTEQSLLNVVPKKYLLYAHHWLV
CCEEEEECCCCCCEEEHHHHHEEHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHE
LHGRYVCKAQKPSCETCIIHDLCEFECKRYKV
EECEEEEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSKKVELIFEKFQQSNPAPKIELNYTNDFTLLVAIVLSARTTDISVNKITKELFSITDT
CCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PEKMLSFGQSELRKCISSIGLYNSKAKNIIGLSKILIERYNSKVPTDFDDLVSLPGVGRK
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCC
SANVFLNSGLGIPTLAVDTHVFRVSNRIGLVKEKDVFKTEQSLLNVVPKKYLLYAHHWLV
CCEEEEECCCCCCEEEHHHHHEEHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHE
LHGRYVCKAQKPSCETCIIHDLCEFECKRYKV
EECEEEEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]