| Definition | Wolbachia sp. wRi, complete genome. |
|---|---|
| Accession | NC_012416 |
| Length | 1,445,873 |
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The map label for this gene is gltX [H]
Identifier: 225630392
GI number: 225630392
Start: 668247
End: 669569
Strand: Direct
Name: gltX [H]
Synonym: WRi_006210
Alternate gene names: 225630392
Gene position: 668247-669569 (Clockwise)
Preceding gene: 225630390
Following gene: 225630393
Centisome position: 46.22
GC content: 36.36
Gene sequence:
>1323_bases ATGCTAACAAGATTCGCTCCAAGCCCAACTGGCTACCTCCACGTAGGAAACGTACGAACTGCCTTGGTTTGTTGGATGTA CACACGCAATCAAAATGGAAAATTTTTACTCCGTTTTGATGATACTGACCTTCAGCGTTCAGATGTCAAATATATAAATG ATATCATACAAGATTTGAAATGGATTGGTATCAATTGGGATGCAAGTTTCAAGCAATCAGAGCGCTTTGAGCGTTACAAT GAGGTGTTTTTGCAGTTAATAAAAGAAGGGCATATTTATGCATGCTATGAAACAAGAGAAGAGTTAGACATTAAACGAAA ATTGCAGTTAAAACAAGGACTTCCCCCTGTATATGACAGAAGCGCGTTACTTCTAACTGAGCAAGAGAAAATTAGTTATG AGCAAGAAGGAAGAAGACCACATTTTAGATTTAAGTTGGATAGAAATGAGGTTGTTAAATGGAATGATGAAGTTAAAGAT GAAATAAATATTGCAACCAGTAGCATAAGTGACCCTGTGGTAAAAAGAGAAGATGGAATTTACACATACATGTTACCTTC TGTTATTGATGATGTTGACTTTAATGTAACCCATGTTGTACGCGGGGAAGATCATGTAACTAATACCGCAGTTCAGCTCC AAATGATTAAAGCATTAAAAGCGAAAATCCCTACATTTGCTCACCTTTCTCTGCTACATTTTGATGATAGCAAGATATCC AAGCGAACCGGCGTGCTGAACATTAAATCTATAAAAGAAGATGAAATTGAACCGATGGCACTAGTTAGTTATTTAGTGAA GCTTGGAACATCCAATCCAATTGAAGCTTGCGCCTGTATGCAATCTTTGATTGATTCATTTAATATTAAAAAATTTAGCT CAGCATCTGTACAATTCAGCTTGAGTGAAATATATAAGCTAAATAGCAAAGTTCTGCAACAAATGCCATTTGAAATGGTG CAAGACCGTTTAAATCAAATTGGAGTGGACTCCTCAGAGTTTTGGTATTTTATAAGGAACAATATAGAAAGGTTTTCTGA GGTGGCCAAGTGGTGGAAAATATGCAAATCTGATATAGAGCCTGTGATTCTTGATAAGGAGCTTATAAAAATTGCGTTCA ATGCATTGCCCCAAGGTGATTGTAATGAAAACACGTTGTCAGAATGGGTCAAAACTATTCGACAAACAGTGGATATAAAG GCAAAAAACCTATTTACGCAGTTGCGTTCAGCTTTAACAGGAACAGAAACAGGACCAGAGCTCGCTAAACTATTAATTTT TATCGGCAAAGAAAATATCATTGCAAGGTTAAAAGAAAAGTGA
Upstream 100 bases:
>100_bases TATTCTTCTCTCGCTCAATTGTATTAGCAGATCTCACTTGCTTTAGCCATATATTTATATAAAATTAAAATAAATTAGAG TAAACAATATTGCTGTTTAA
Downstream 100 bases:
>100_bases AAACTAAAGAATTATAGCTAATGCAATCCCTACGTCATACCGCGATTCATTCGCGGTATCTCAGCCGCTAACAAGAGATC CCGCTAACAAGCAGCGGGAT
Product: glutamyl-tRNA synthetase
Products: NA
Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]
Number of amino acids: Translated: 440; Mature: 440
Protein sequence:
>440_residues MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK
Sequences:
>Translated_440_residues MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK >Mature_440_residues MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK
Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]
COG id: COG0008
COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]
Homologues:
Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=26.8993839835729, Blast_Score=164, Evalue=1e-40, Organism=Homo sapiens, GI62241042, Length=242, Percent_Identity=26.4462809917355, Blast_Score=72, Evalue=1e-12, Organism=Escherichia coli, GI1788743, Length=458, Percent_Identity=31.6593886462882, Blast_Score=243, Evalue=2e-65, Organism=Escherichia coli, GI226510925, Length=274, Percent_Identity=27.007299270073, Blast_Score=101, Evalue=9e-23, Organism=Escherichia coli, GI1786895, Length=141, Percent_Identity=30.4964539007092, Blast_Score=76, Evalue=4e-15, Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=36, Blast_Score=153, Evalue=2e-37, Organism=Saccharomyces cerevisiae, GI6324540, Length=327, Percent_Identity=32.1100917431193, Blast_Score=163, Evalue=5e-41, Organism=Saccharomyces cerevisiae, GI6321192, Length=260, Percent_Identity=25.7692307692308, Blast_Score=80, Evalue=7e-16, Organism=Saccharomyces cerevisiae, GI6324742, Length=127, Percent_Identity=34.6456692913386, Blast_Score=78, Evalue=2e-15, Organism=Drosophila melanogaster, GI24665321, Length=481, Percent_Identity=31.1850311850312, Blast_Score=184, Evalue=1e-46, Organism=Drosophila melanogaster, GI24649466, Length=243, Percent_Identity=26.3374485596708, Blast_Score=74, Evalue=1e-13,
Paralogues:
None
Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008925 - InterPro: IPR020751 - InterPro: IPR001412 - InterPro: IPR004527 - InterPro: IPR000924 - InterPro: IPR020061 - InterPro: IPR020058 - InterPro: IPR014729 [H]
Pfam domain/function: PF00749 tRNA-synt_1c [H]
EC number: =6.1.1.17 [H]
Molecular weight: Translated: 50996; Mature: 50996
Theoretical pI: Translated: 7.15; Mature: 7.15
Prosite motif: PS00178 AA_TRNA_LIGASE_I
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLK CCCCCCCCCCCEEEECCHHEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHE WIGINWDASFKQSERFERYNEVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDR EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCC SALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKDEINIATSSISDPVVKREDGI CEEEEECHHHHCHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCE YTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS EEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCHHHH KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFS HHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE LSEIYKLNSKVLQQMPFEMVQDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIE HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC PVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIKAKNLFTQLRSALTGTETGPE CEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHH LAKLLIFIGKENIIARLKEK HHHHHHHHCCCHHHHHHCCC >Mature Secondary Structure MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLK CCCCCCCCCCCEEEECCHHEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHE WIGINWDASFKQSERFERYNEVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDR EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCC SALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKDEINIATSSISDPVVKREDGI CEEEEECHHHHCHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCE YTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS EEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCHHHH KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFS HHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE LSEIYKLNSKVLQQMPFEMVQDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIE HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC PVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIKAKNLFTQLRSALTGTETGPE CEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHH LAKLLIFIGKENIIARLKEK HHHHHHHHCCCHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA