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The map label for this gene is dapA [H]

Identifier: 225630390

GI number: 225630390

Start: 665142

End: 666005

Strand: Direct

Name: dapA [H]

Synonym: WRi_006190

Alternate gene names: 225630390

Gene position: 665142-666005 (Clockwise)

Preceding gene: 225630389

Following gene: 225630392

Centisome position: 46.0

GC content: 41.78

Gene sequence:

>864_bases
ATGCAAGTTAAAGCTGAAAATGGCGTAGTGTTGCTGGGTAGCACAGGTGAGAGCCTATCGCTTACTGATTCTGAAAAGCG
TACATTAGTTGAATTCGTATGTAAGCTAAAATTAAATACGAAAATTATAATTGGCGTACCAGGAGTGAATCTATATCAGA
CTCTTGAATGGCTTGATTTTTGCAAGGGTATGCCTATTCATGGCTATCTAATGACAACTCCCATTTATGCAAAGCCCGGA
ATTATGGGGCAGACTTTATGGTTTGAAAAGCTGCTTGAGAAAGCACATGTGCCGGCTATGTTTTACAATATTCCATCAAG
AGCAGGAGTGAGTCTCTACGCTGAAACTATACGCAACTTATCCAGCGACGAAAAATTTTGGGCTATCAAAGATTCAAGTG
GCACTGTTGATACTTTAGCTCAGTATAAGAAAGTTGCACCAAATATTGAGGTCTTTTGCGGAGATGATAATATGATATCC
GATATGGCCGCTTATGGTGCGGCTGGCCTGGTTTCCGTTGCTTCCAATGTTTGGCCACGCATAGCGCATGAATATGTTAA
GAAATGTCTGAACGACGAACTCTCTTCACTGTCATTCCAGCGCGTGACGCTGGAATCCAGAAGGAAACCTGTTCAAATGA
CAGAAAATGACATGAAAAGTTCAACAGATATCTGGCAACAGGCTTGCGAAGCGTTATTTACTGCCAGCAACCCAATACCC
ACTAAAGCACTTTTGCATGATATTGGGCTCATAGAACACAAAACTGTCCGCCTACCGCTCAGCACAGAAGACTTGCCTTC
TGTTGAAAAATTGAGGCAAGTCAATAAAATGATTCTTGGATGGAAAGAATTGGCTACTCTCTAA

Upstream 100 bases:

>100_bases
ATTTAAATTGAAATCTACATTTTTATGGACTGCTTGTGTTACTCCTTTTAATTGCAATGGAGATAGCATAGATTATAGCA
GTTTGCAGCGTTTACTGACA

Downstream 100 bases:

>100_bases
ATAGTCTTGCAACTTTGATTTAATATACAATCCAAGTTTCATTTGAACACTCCCAAACTTATCACTTTATGCCACTGGTC
TTCATCTAAAATTTCTACAC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQVKAENGVVLLGSTGESLSLTDSEKRTLVEFVCKLKLNTKIIIGVPGVNLYQTLEWLDFCKGMPIHGYLMTTPIYAKPG
IMGQTLWFEKLLEKAHVPAMFYNIPSRAGVSLYAETIRNLSSDEKFWAIKDSSGTVDTLAQYKKVAPNIEVFCGDDNMIS
DMAAYGAAGLVSVASNVWPRIAHEYVKKCLNDELSSLSFQRVTLESRRKPVQMTENDMKSSTDIWQQACEALFTASNPIP
TKALLHDIGLIEHKTVRLPLSTEDLPSVEKLRQVNKMILGWKELATL

Sequences:

>Translated_287_residues
MQVKAENGVVLLGSTGESLSLTDSEKRTLVEFVCKLKLNTKIIIGVPGVNLYQTLEWLDFCKGMPIHGYLMTTPIYAKPG
IMGQTLWFEKLLEKAHVPAMFYNIPSRAGVSLYAETIRNLSSDEKFWAIKDSSGTVDTLAQYKKVAPNIEVFCGDDNMIS
DMAAYGAAGLVSVASNVWPRIAHEYVKKCLNDELSSLSFQRVTLESRRKPVQMTENDMKSSTDIWQQACEALFTASNPIP
TKALLHDIGLIEHKTVRLPLSTEDLPSVEKLRQVNKMILGWKELATL
>Mature_287_residues
MQVKAENGVVLLGSTGESLSLTDSEKRTLVEFVCKLKLNTKIIIGVPGVNLYQTLEWLDFCKGMPIHGYLMTTPIYAKPG
IMGQTLWFEKLLEKAHVPAMFYNIPSRAGVSLYAETIRNLSSDEKFWAIKDSSGTVDTLAQYKKVAPNIEVFCGDDNMIS
DMAAYGAAGLVSVASNVWPRIAHEYVKKCLNDELSSLSFQRVTLESRRKPVQMTENDMKSSTDIWQQACEALFTASNPIP
TKALLHDIGLIEHKTVRLPLSTEDLPSVEKLRQVNKMILGWKELATL

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family [H]

Homologues:

Organism=Escherichia coli, GI1788823, Length=259, Percent_Identity=27.4131274131274, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786463, Length=207, Percent_Identity=24.1545893719807, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082415, Length=185, Percent_Identity=23.2432432432432, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.2.1.52 [H]

Molecular weight: Translated: 32002; Mature: 32002

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00665 DHDPS_1 ; PS00666 DHDPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKAENGVVLLGSTGESLSLTDSEKRTLVEFVCKLKLNTKIIIGVPGVNLYQTLEWLDF
CEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
CKGMPIHGYLMTTPIYAKPGIMGQTLWFEKLLEKAHVPAMFYNIPSRAGVSLYAETIRNL
HCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCC
SSDEKFWAIKDSSGTVDTLAQYKKVAPNIEVFCGDDNMISDMAAYGAAGLVSVASNVWPR
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
IAHEYVKKCLNDELSSLSFQRVTLESRRKPVQMTENDMKSSTDIWQQACEALFTASNPIP
HHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TKALLHDIGLIEHKTVRLPLSTEDLPSVEKLRQVNKMILGWKELATL
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVKAENGVVLLGSTGESLSLTDSEKRTLVEFVCKLKLNTKIIIGVPGVNLYQTLEWLDF
CEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHH
CKGMPIHGYLMTTPIYAKPGIMGQTLWFEKLLEKAHVPAMFYNIPSRAGVSLYAETIRNL
HCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCC
SSDEKFWAIKDSSGTVDTLAQYKKVAPNIEVFCGDDNMISDMAAYGAAGLVSVASNVWPR
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
IAHEYVKKCLNDELSSLSFQRVTLESRRKPVQMTENDMKSSTDIWQQACEALFTASNPIP
HHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TKALLHDIGLIEHKTVRLPLSTEDLPSVEKLRQVNKMILGWKELATL
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA