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The map label for this gene is ribF [H]

Identifier: 225630376

GI number: 225630376

Start: 648084

End: 649016

Strand: Direct

Name: ribF [H]

Synonym: WRi_006020

Alternate gene names: 225630376

Gene position: 648084-649016 (Clockwise)

Preceding gene: 225630370

Following gene: 225630377

Centisome position: 44.82

GC content: 31.51

Gene sequence:

>933_bases
ATGAAAATTATTTATAATTGTGAGCAAGGAATAGAAAATAATATAGCACTAACCTTCGGAAATTTCGATGGCATTCATTT
AGGACACGAGTTTGCAATTTCTACTCTAAAGAAGGTAGCACAAGAAAGAGAATTACCCTCTGCAGTTTTGACTTTTGAGC
CTCACCCATCAACTGTTTTATTTGGTAGAAACAATTTTAGGTTAATAGACCAAGAACAAAAAAAGGAACTAATCAGCAGC
TATGGTATAGATTACTTATATATTATTAATTTTAATAGAGGTTTTTCTGAGGTTAGCTGCGATGACTTTATCAGTGAGAT
TTTAATAGATAAATATGGCGCTAAACACATAATTGTCGGAGAAAATTGCACTTTTGGTCATAAACGATTGGGTAATATAT
TAACTCTAGAGAAATATTCTGAAATATATGGATACTCTTTGACTAAATTAGAGCCATTAACAATTGACGGCAAAATTTGC
TCTTCTTCTTCAATAAGGGAGTATGTACAAAGGGGCGAAATAGAAATTGCTAATAAATTGCTCGGCAGGCCTTATCAAGT
TTCTGGTGTTGTGACAAAAGGTGCATGTAGAGGTAGAGAAATAGGATTTCCAACCATAAATATTCCTATAGAAGATTGCA
TGATAAAACCTAAGTTTGGCACATATTACGCTAGAGTGACTTTTCCTGATAGTAATCCAAATTGGTTATATGGAGTAGTC
AATATTGGTATGAGACCTACATTTAAGGATCTGAAAAAGCCTATAATAGAAATGCACATATTCGATTTTGATGAAGACGT
ATATAGTCATAAAGTCAATATACAACTTTTAAAATTCATAAGGTCAGAAAAAAAATTCCATAGTATTGAGGAGTTAACAA
AACAGATAAATTATGATATACTTAAAGTTTATAGGTTAAAGGCAAATTTATGA

Upstream 100 bases:

>100_bases
CCCTTCTTTACATATATCACAACATTTTTCACAATTTATCTCCGTAGCTTACTAAAATATAGTTATTATATATAAGAAAC
ATGTAAAAACATCAAATTGT

Downstream 100 bases:

>100_bases
AAAAGTTATGCTTAGTTGTTATATTAATTATAGTATTGATTGACCAAACGAGCAAATTGTACATAAACTCATTGATTGAT
GAAGGAGAATCAATTGAGAT

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKIIYNCEQGIENNIALTFGNFDGIHLGHEFAISTLKKVAQERELPSAVLTFEPHPSTVLFGRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFISEILIDKYGAKHIIVGENCTFGHKRLGNILTLEKYSEIYGYSLTKLEPLTIDGKIC
SSSSIREYVQRGEIEIANKLLGRPYQVSGVVTKGACRGREIGFPTINIPIEDCMIKPKFGTYYARVTFPDSNPNWLYGVV
NIGMRPTFKDLKKPIIEMHIFDFDEDVYSHKVNIQLLKFIRSEKKFHSIEELTKQINYDILKVYRLKANL

Sequences:

>Translated_310_residues
MKIIYNCEQGIENNIALTFGNFDGIHLGHEFAISTLKKVAQERELPSAVLTFEPHPSTVLFGRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFISEILIDKYGAKHIIVGENCTFGHKRLGNILTLEKYSEIYGYSLTKLEPLTIDGKIC
SSSSIREYVQRGEIEIANKLLGRPYQVSGVVTKGACRGREIGFPTINIPIEDCMIKPKFGTYYARVTFPDSNPNWLYGVV
NIGMRPTFKDLKKPIIEMHIFDFDEDVYSHKVNIQLLKFIRSEKKFHSIEELTKQINYDILKVYRLKANL
>Mature_310_residues
MKIIYNCEQGIENNIALTFGNFDGIHLGHEFAISTLKKVAQERELPSAVLTFEPHPSTVLFGRNNFRLIDQEQKKELISS
YGIDYLYIINFNRGFSEVSCDDFISEILIDKYGAKHIIVGENCTFGHKRLGNILTLEKYSEIYGYSLTKLEPLTIDGKIC
SSSSIREYVQRGEIEIANKLLGRPYQVSGVVTKGACRGREIGFPTINIPIEDCMIKPKFGTYYARVTFPDSNPNWLYGVV
NIGMRPTFKDLKKPIIEMHIFDFDEDVYSHKVNIQLLKFIRSEKKFHSIEELTKQINYDILKVYRLKANL

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=300, Percent_Identity=34.6666666666667, Blast_Score=187, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35632; Mature: 35632

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIYNCEQGIENNIALTFGNFDGIHLGHEFAISTLKKVAQERELPSAVLTFEPHPSTVL
CEEEEECCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
FGRNNFRLIDQEQKKELISSYGIDYLYIINFNRGFSEVSCDDFISEILIDKYGAKHIIVG
EECCCCEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
ENCTFGHKRLGNILTLEKYSEIYGYSLTKLEPLTIDGKICSSSSIREYVQRGEIEIANKL
CCCCCCHHHHCCEEEHHHHHHHHCCEECCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHH
LGRPYQVSGVVTKGACRGREIGFPTINIPIEDCMIKPKFGTYYARVTFPDSNPNWLYGVV
CCCCEEEEEEEECCCCCCCCCCCCEEECCHHHCEECCCCCCEEEEEEECCCCCCEEEEEE
NIGMRPTFKDLKKPIIEMHIFDFDEDVYSHKVNIQLLKFIRSEKKFHSIEELTKQINYDI
ECCCCCCHHHHHHHHEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LKVYRLKANL
EEEEEEECCC
>Mature Secondary Structure
MKIIYNCEQGIENNIALTFGNFDGIHLGHEFAISTLKKVAQERELPSAVLTFEPHPSTVL
CEEEEECCCCCCCCEEEEECCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
FGRNNFRLIDQEQKKELISSYGIDYLYIINFNRGFSEVSCDDFISEILIDKYGAKHIIVG
EECCCCEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEC
ENCTFGHKRLGNILTLEKYSEIYGYSLTKLEPLTIDGKICSSSSIREYVQRGEIEIANKL
CCCCCCHHHHCCEEEHHHHHHHHCCEECCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHH
LGRPYQVSGVVTKGACRGREIGFPTINIPIEDCMIKPKFGTYYARVTFPDSNPNWLYGVV
CCCCEEEEEEEECCCCCCCCCCCCEEECCHHHCEECCCCCCEEEEEEECCCCCCEEEEEE
NIGMRPTFKDLKKPIIEMHIFDFDEDVYSHKVNIQLLKFIRSEKKFHSIEELTKQINYDI
ECCCCCCHHHHHHHHEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LKVYRLKANL
EEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9454067; 9108293; 9384377 [H]