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The map label for this gene is lspA [H]

Identifier: 225630377

GI number: 225630377

Start: 649013

End: 649489

Strand: Direct

Name: lspA [H]

Synonym: WRi_006030

Alternate gene names: 225630377

Gene position: 649013-649489 (Clockwise)

Preceding gene: 225630376

Following gene: 225630378

Centisome position: 44.89

GC content: 31.03

Gene sequence:

>477_bases
ATGAAAAAGTTATGCTTAGTTGTTATATTAATTATAGTATTGATTGACCAAACGAGCAAATTGTACATAAACTCATTGAT
TGATGAAGGAGAATCAATTGAGATCGCTAGTTTTATAAAACTAGTTGAAGTTTGGAATTCAGGTATTAGTTTTGGAATGT
GTAGCGCTTTACCACATGGCGGTTTCTTTTTTTCAGCATTTTCAATACTAATAATTGGTATACTTGCATATTTGATATAC
AAGTCTGACGATCAGTCAACTTACCTTGGTTTTTCTCTGATGATTGGTGGAGCAATCGGAAACGTAGTTGATAGGATCTA
TTGGGGAGCTGTATATGATTTCATATATTTTCATATTGATGATTGGTATTGGCCAGCCTTTAATTTAGCAGATTTATCTA
TAGTTTGTGGAATGTTTACATTGTTATATAAGTGGTATATATACGATATGCTTATTTCTAAACAAAATGAGGAATAA

Upstream 100 bases:

>100_bases
TAAAATTCATAAGGTCAGAAAAAAAATTCCATAGTATTGAGGAGTTAACAAAACAGATAAATTATGATATACTTAAAGTT
TATAGGTTAAAGGCAAATTT

Downstream 100 bases:

>100_bases
AATAATTGTTTTTATATTAGGTCGAAATAATGCTTCATAAGTTTTTTAGTTTTCTTATACTACCTGTACTTTTTTTTACT
TACCCAATTTCTTCGAATGC

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKKLCLVVILIIVLIDQTSKLYINSLIDEGESIEIASFIKLVEVWNSGISFGMCSALPHGGFFFSAFSILIIGILAYLIY
KSDDQSTYLGFSLMIGGAIGNVVDRIYWGAVYDFIYFHIDDWYWPAFNLADLSIVCGMFTLLYKWYIYDMLISKQNEE

Sequences:

>Translated_158_residues
MKKLCLVVILIIVLIDQTSKLYINSLIDEGESIEIASFIKLVEVWNSGISFGMCSALPHGGFFFSAFSILIIGILAYLIY
KSDDQSTYLGFSLMIGGAIGNVVDRIYWGAVYDFIYFHIDDWYWPAFNLADLSIVCGMFTLLYKWYIYDMLISKQNEE
>Mature_158_residues
MKKLCLVVILIIVLIDQTSKLYINSLIDEGESIEIASFIKLVEVWNSGISFGMCSALPHGGFFFSAFSILIIGILAYLIY
KSDDQSTYLGFSLMIGGAIGNVVDRIYWGAVYDFIYFHIDDWYWPAFNLADLSIVCGMFTLLYKWYIYDMLISKQNEE

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins [H]

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family [H]

Homologues:

Organism=Escherichia coli, GI1786210, Length=135, Percent_Identity=32.5925925925926, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001872 [H]

Pfam domain/function: PF01252 Peptidase_A8 [H]

EC number: =3.4.23.36 [H]

Molecular weight: Translated: 18038; Mature: 18038

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS00855 SPASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLCLVVILIIVLIDQTSKLYINSLIDEGESIEIASFIKLVEVWNSGISFGMCSALPHG
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCC
GFFFSAFSILIIGILAYLIYKSDDQSTYLGFSLMIGGAIGNVVDRIYWGAVYDFIYFHID
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DWYWPAFNLADLSIVCGMFTLLYKWYIYDMLISKQNEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLCLVVILIIVLIDQTSKLYINSLIDEGESIEIASFIKLVEVWNSGISFGMCSALPHG
CHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCC
GFFFSAFSILIIGILAYLIYKSDDQSTYLGFSLMIGGAIGNVVDRIYWGAVYDFIYFHID
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DWYWPAFNLADLSIVCGMFTLLYKWYIYDMLISKQNEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA