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The map label for this gene is aprE [H]

Identifier: 225630268

GI number: 225630268

Start: 531772

End: 533364

Strand: Reverse

Name: aprE [H]

Synonym: WRi_004860

Alternate gene names: 225630268

Gene position: 533364-531772 (Counterclockwise)

Preceding gene: 225630271

Following gene: 225630267

Centisome position: 36.89

GC content: 35.78

Gene sequence:

>1593_bases
ATGGAATGCTTAAATTATAGATTCTTTGTTTTTGTGTGTTATCTTGAGTTAACACAAATTCACAGTAGTATTATGATAAG
TAAGTTAAAAAAGCTTAGCGCTGGATTTTCTTTGACAGGCTTGATTGGCCATGGTGATAATATAAATATGCAAGGAGCAA
AAAATAAAAAGAAGAAGTACCCAAAATTTTTGGATAAGACGTTTGCATTGATTGATGCAGTGATAAATTTTATTTTAAAA
CGCGAAAGTAATAACGTAAATGAAGTCCTGAAAGTAACATGGGGACCACTATTTTTTGGTCTGGTAGTAATACTCATCTT
TTTTGGGATAGGTGGTATTTGGTCGGCTATAGCTCCAATTGACGGTGCGGTGCATGCAAGTGGAGAAGTTATTGTATCTT
CAAATAGGAAAATAGTTCAACACTTGGGTGGAGGAATAATAAGCAAAATTTTAGTAAAAGAGAGCCAAGCGGTTAAAAAA
GATGAGCCTTTAGTTTTATTAAGTGATGTTAACGAGAAGGCGAATTTAAGTATCATCAAAGAGAAGCTCTTATCATTTTT
AGCAACAGAAGCAAGGCTTCTTGCAATCAGAGTAGATTTGGACACACTAGAATTTTCTGATGAGGTTAAAAAATTATCTA
ACGATGAACTTGTAAATAAAGTGATAAAGAATCAAGTAAAGTTGTTCAACTCTCAGAGAAAGAGCATATTGGGAAAAACA
GACATACTGCAACAACGTATAAAGCAGTTAAATGATGAATTAGCGGGGCTAAACTTTCAACTAAATGCAGCTCATAAGCA
ATATGACTTGATAACTGAGGAGTTGGAAACAAAAAGACAATTACTTGATAGTGGCCATATAAGCAAACCACACATTTTAG
CTTTAGAAAAACAGTTTGCTGAAATTGAAGGCAGAGTTGGACATTACCGTTCTGCAATATCTCAAGTGCAGCAAAAAATT
GGAGAGAACGAATTAGAAATTATAAACGTAAAAAATGATTCTCAAGAAAGAGCAAACGCTGAACTTAAGGAAGTTAGTAC
GTCTATTGCAGACTTGAAAGAGAGGCTGATGGTTGCTGAAGATTCATTGGCACGTACAATAATTAAATCGCCTCAAGATG
GGATAGTTACAGATATAAGATACCATACTGAAGGTGGTGTTATACAATCTGGAGTTCCGATCATGAGCGTAGTGCCATCG
GATGATGACCTAATAATAGATGCTAAAATTCAGACCAGAAACATAGAAGAGGTACTGTCAGCACAAAAGAAAGATAGCAA
CATAGTCTCTATTGATGGGCTAGAAGGGCTAAAGGTTAAAGTCCGGTTAAGTGCTTACAGTGCACGTCGTTTAAGTTTAA
TTAGTGGTATAGTAAGCCATATTTCTCCTGACGCTCTTGATGATCCAAGACTAGGGCGTTATTATTCAGTGCGCGTAGTG
ATACCAAAGTCAGAGCTTGCTCAATTTAAAAATGTATACCTATACCCTGGTATGCCAGCAGAAGTGTACATAGTTACTCA
ATCCCGTACTCTTTTATCATTCTTGTTTACGCCTATAATTGCAACAGTCGATAGGTCTTTTATAGAAAGGTAG

Upstream 100 bases:

>100_bases
AAGGAAAAGTTATAATAAAGTTAAACGTCAAAAAATTTTAAAAACATAATTGATGCAGGAATTGTTACTAAATAGTTTAT
TCTGGTGCTCTACAGTCTAA

Downstream 100 bases:

>100_bases
CTCACTCGTTAAAGTAGTAGTGAAAGTGCCGTAATAAAATTTAGGTACATAATTAGACAATAGGCTTCTTGACCTTACCC
AGAAAAAGTGGAGAGAAAGA

Product: secretion protein, HlyD family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MECLNYRFFVFVCYLELTQIHSSIMISKLKKLSAGFSLTGLIGHGDNINMQGAKNKKKKYPKFLDKTFALIDAVINFILK
RESNNVNEVLKVTWGPLFFGLVVILIFFGIGGIWSAIAPIDGAVHASGEVIVSSNRKIVQHLGGGIISKILVKESQAVKK
DEPLVLLSDVNEKANLSIIKEKLLSFLATEARLLAIRVDLDTLEFSDEVKKLSNDELVNKVIKNQVKLFNSQRKSILGKT
DILQQRIKQLNDELAGLNFQLNAAHKQYDLITEELETKRQLLDSGHISKPHILALEKQFAEIEGRVGHYRSAISQVQQKI
GENELEIINVKNDSQERANAELKEVSTSIADLKERLMVAEDSLARTIIKSPQDGIVTDIRYHTEGGVIQSGVPIMSVVPS
DDDLIIDAKIQTRNIEEVLSAQKKDSNIVSIDGLEGLKVKVRLSAYSARRLSLISGIVSHISPDALDDPRLGRYYSVRVV
IPKSELAQFKNVYLYPGMPAEVYIVTQSRTLLSFLFTPIIATVDRSFIER

Sequences:

>Translated_530_residues
MECLNYRFFVFVCYLELTQIHSSIMISKLKKLSAGFSLTGLIGHGDNINMQGAKNKKKKYPKFLDKTFALIDAVINFILK
RESNNVNEVLKVTWGPLFFGLVVILIFFGIGGIWSAIAPIDGAVHASGEVIVSSNRKIVQHLGGGIISKILVKESQAVKK
DEPLVLLSDVNEKANLSIIKEKLLSFLATEARLLAIRVDLDTLEFSDEVKKLSNDELVNKVIKNQVKLFNSQRKSILGKT
DILQQRIKQLNDELAGLNFQLNAAHKQYDLITEELETKRQLLDSGHISKPHILALEKQFAEIEGRVGHYRSAISQVQQKI
GENELEIINVKNDSQERANAELKEVSTSIADLKERLMVAEDSLARTIIKSPQDGIVTDIRYHTEGGVIQSGVPIMSVVPS
DDDLIIDAKIQTRNIEEVLSAQKKDSNIVSIDGLEGLKVKVRLSAYSARRLSLISGIVSHISPDALDDPRLGRYYSVRVV
IPKSELAQFKNVYLYPGMPAEVYIVTQSRTLLSFLFTPIIATVDRSFIER
>Mature_530_residues
MECLNYRFFVFVCYLELTQIHSSIMISKLKKLSAGFSLTGLIGHGDNINMQGAKNKKKKYPKFLDKTFALIDAVINFILK
RESNNVNEVLKVTWGPLFFGLVVILIFFGIGGIWSAIAPIDGAVHASGEVIVSSNRKIVQHLGGGIISKILVKESQAVKK
DEPLVLLSDVNEKANLSIIKEKLLSFLATEARLLAIRVDLDTLEFSDEVKKLSNDELVNKVIKNQVKLFNSQRKSILGKT
DILQQRIKQLNDELAGLNFQLNAAHKQYDLITEELETKRQLLDSGHISKPHILALEKQFAEIEGRVGHYRSAISQVQQKI
GENELEIINVKNDSQERANAELKEVSTSIADLKERLMVAEDSLARTIIKSPQDGIVTDIRYHTEGGVIQSGVPIMSVVPS
DDDLIIDAKIQTRNIEEVLSAQKKDSNIVSIDGLEGLKVKVRLSAYSARRLSLISGIVSHISPDALDDPRLGRYYSVRVV
IPKSELAQFKNVYLYPGMPAEVYIVTQSRTLLSFLFTPIIATVDRSFIER

Specific function: Involved in the secretion of alkaline protease [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR010129 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 59175; Mature: 59175

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECLNYRFFVFVCYLELTQIHSSIMISKLKKLSAGFSLTGLIGHGDNINMQGAKNKKKKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHC
PKFLDKTFALIDAVINFILKRESNNVNEVLKVTWGPLFFGLVVILIFFGIGGIWSAIAPI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGAVHASGEVIVSSNRKIVQHLGGGIISKILVKESQAVKKDEPLVLLSDVNEKANLSIIK
CCCEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
EKLLSFLATEARLLAIRVDLDTLEFSDEVKKLSNDELVNKVIKNQVKLFNSQRKSILGKT
HHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
DILQQRIKQLNDELAGLNFQLNAAHKQYDLITEELETKRQLLDSGHISKPHILALEKQFA
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
EIEGRVGHYRSAISQVQQKIGENELEIINVKNDSQERANAELKEVSTSIADLKERLMVAE
HHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLARTIIKSPQDGIVTDIRYHTEGGVIQSGVPIMSVVPSDDDLIIDAKIQTRNIEEVLS
HHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCEEEEECCCCCEEEEEEECCCCHHHHHH
AQKKDSNIVSIDGLEGLKVKVRLSAYSARRLSLISGIVSHISPDALDDPRLGRYYSVRVV
HHCCCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
IPKSELAQFKNVYLYPGMPAEVYIVTQSRTLLSFLFTPIIATVDRSFIER
ECHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MECLNYRFFVFVCYLELTQIHSSIMISKLKKLSAGFSLTGLIGHGDNINMQGAKNKKKKY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHC
PKFLDKTFALIDAVINFILKRESNNVNEVLKVTWGPLFFGLVVILIFFGIGGIWSAIAPI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGAVHASGEVIVSSNRKIVQHLGGGIISKILVKESQAVKKDEPLVLLSDVNEKANLSIIK
CCCEECCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
EKLLSFLATEARLLAIRVDLDTLEFSDEVKKLSNDELVNKVIKNQVKLFNSQRKSILGKT
HHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
DILQQRIKQLNDELAGLNFQLNAAHKQYDLITEELETKRQLLDSGHISKPHILALEKQFA
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
EIEGRVGHYRSAISQVQQKIGENELEIINVKNDSQERANAELKEVSTSIADLKERLMVAE
HHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLARTIIKSPQDGIVTDIRYHTEGGVIQSGVPIMSVVPSDDDLIIDAKIQTRNIEEVLS
HHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCEEEEECCCCCEEEEEEECCCCHHHHHH
AQKKDSNIVSIDGLEGLKVKVRLSAYSARRLSLISGIVSHISPDALDDPRLGRYYSVRVV
HHCCCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
IPKSELAQFKNVYLYPGMPAEVYIVTQSRTLLSFLFTPIIATVDRSFIER
ECHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]