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The map label for this gene is groEL [H]

Identifier: 225630245

GI number: 225630245

Start: 494303

End: 495952

Strand: Reverse

Name: groEL [H]

Synonym: WRi_004590

Alternate gene names: 225630245

Gene position: 495952-494303 (Counterclockwise)

Preceding gene: 225630246

Following gene: 225630244

Centisome position: 34.3

GC content: 38.06

Gene sequence:

>1650_bases
ATGACTAATGTAGTAGTATCAGGTGAGCAGTTGCAAGAAGCCTTTCGTGAAGTCGCAGCAATAGTAGACTCAACGGTGGC
AATAACTGCGGGACCTAGAGGAAAAACAGTAGGGATTAATAAGCCCTATGGAGCACCAGAAATTACAAAAGATGGTTATA
AGGTGATGAAGGGTATCAAGCCTGAAAAACCATTAAACGCTGCGATAGCAAGCATCTTTGCACAAAGTTGTTCTCAATGT
AACGATAAAGTTGGTGATGGTACAACAACGTGCTCAATACTAACTAGCAACATGATAATGGAAGCTTCAAAATCAATTGC
TGCTGGAAACGATCGTGTTGGTATTAAAAACGGAATACAGAAGGCAAAAGATGTAATATTAAAGGAAATTGCGTCAATGT
CTCGTACAATTTCTCTAGAGAAAATAGACGAAGTGGCACAAGTTGCAATAATCTCTGCAAATGGTGATAAGGATATAGGT
AACAGTATCGCTGATTCCGTGAAAAAAGTTGGAAAAGAGGGTGTAATAACTGTTGAAGAGAGTAAAGGTTCAAAAGAGTT
AGAAGTTGAGCTGACTACTGGCATGCAATTTGATCGCGGTTATCTCTCTCCGTATTTTATTACAAATAATGAAAAAATGA
TCGTGGAGCTTGATAATCCTTATCTATTAATTACAGAGAAAAAATTAAATATTATTCAACCTTTACTTCCTGTTCTTGAA
GCTATTGTTAAATCTGGTAAACCTTTGGTTATTATTGCAGAGGATATCGAAGGTGAAGCATTAAGCACTTTAGTTATCAA
TAAATTGCGTGGTGGTTTAAAAGTTGCTGCAGTAAAAGCTCCAGGTTTTGGTGACAGAAGAAAGGAGATGCTCGAAGACA
TAGCAACTTTGACTGGTGCTAAGTACGTCATAAAAGATGAACTTGGAATCAAGATGGAAGATCTCACGCTTGATGATCTT
GGTACGGCTAAAAATGTTAAAATTACTAAAGATAATACCACAGTTGTCAGCGAAAATAGCGATTCTGACAGTGTGAAAGC
TAGAATCGAGCAGATTAAATCTCAAATTGAAACTTCAACTTCTGATTATGATAAAGAAAAGCTAAGAGAACGTTTAGCGA
AATTATCAGGTGGCGTTGCTGTGCTCAAAGTTGGTGGAGCAACTGAAGTGGAAGTTAAAGAACGTAGAGATAGAGTCGAA
GATGCGCTGCATGCAACAAGAGCTGCAATTGAAGAAGGTATAGTTCCAGGTGGTGGGGTTGCGCTTCTTTATGCTTCATC
TGTTCTCGACAAATTAAAAGGTGCAAGTGACGAGGAACAAATAGGCATAAACATTATCAAGAAAGTTCTCAGTGCTCCAA
TTAGAAGGTTAGTTAAAAATGCTGGTCTTGAATCTGCTGTTATAATTGACTATTTGATTAAGCAGAATGATAAAGAGCTT
ATATACAACGTTGAGGCTATGAATTACGCTAATGCATTTACAGCTGGTGTGATTGATCCAGCAAAAGTGGTGCGTATTGC
TTTTGAAACAGCGGTATCTGTTGCGAGTGTGCTGATAACTACTGAATCTATGATAGTTGATGTACCAAGCAAAGAAAATG
CTTCATCTCCTATGGGTGCAGGAGAAATGAGTGGCATGGGTGGATTCTAA

Upstream 100 bases:

>100_bases
CAGTCTTTATTTTACTAAACTTAAACAAAGCGCAACATGACGCATTGTGAATGATTTTATTATAGTTTTCTAAATTTTTT
ATTAACAAGAGGTGATAAAA

Downstream 100 bases:

>100_bases
GTAGAATGAAACCGTGGAGCAATTGCTCCACGGTAGTTCCAAAAAATCTCACATTTTACTATTCGTTAAAGGTAATACGT
TTGGTGCAGAAATGCACTAC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTNVVVSGEQLQEAFREVAAIVDSTVAITAGPRGKTVGINKPYGAPEITKDGYKVMKGIKPEKPLNAAIASIFAQSCSQC
NDKVGDGTTTCSILTSNMIMEASKSIAAGNDRVGIKNGIQKAKDVILKEIASMSRTISLEKIDEVAQVAIISANGDKDIG
NSIADSVKKVGKEGVITVEESKGSKELEVELTTGMQFDRGYLSPYFITNNEKMIVELDNPYLLITEKKLNIIQPLLPVLE
AIVKSGKPLVIIAEDIEGEALSTLVINKLRGGLKVAAVKAPGFGDRRKEMLEDIATLTGAKYVIKDELGIKMEDLTLDDL
GTAKNVKITKDNTTVVSENSDSDSVKARIEQIKSQIETSTSDYDKEKLRERLAKLSGGVAVLKVGGATEVEVKERRDRVE
DALHATRAAIEEGIVPGGGVALLYASSVLDKLKGASDEEQIGINIIKKVLSAPIRRLVKNAGLESAVIIDYLIKQNDKEL
IYNVEAMNYANAFTAGVIDPAKVVRIAFETAVSVASVLITTESMIVDVPSKENASSPMGAGEMSGMGGF

Sequences:

>Translated_549_residues
MTNVVVSGEQLQEAFREVAAIVDSTVAITAGPRGKTVGINKPYGAPEITKDGYKVMKGIKPEKPLNAAIASIFAQSCSQC
NDKVGDGTTTCSILTSNMIMEASKSIAAGNDRVGIKNGIQKAKDVILKEIASMSRTISLEKIDEVAQVAIISANGDKDIG
NSIADSVKKVGKEGVITVEESKGSKELEVELTTGMQFDRGYLSPYFITNNEKMIVELDNPYLLITEKKLNIIQPLLPVLE
AIVKSGKPLVIIAEDIEGEALSTLVINKLRGGLKVAAVKAPGFGDRRKEMLEDIATLTGAKYVIKDELGIKMEDLTLDDL
GTAKNVKITKDNTTVVSENSDSDSVKARIEQIKSQIETSTSDYDKEKLRERLAKLSGGVAVLKVGGATEVEVKERRDRVE
DALHATRAAIEEGIVPGGGVALLYASSVLDKLKGASDEEQIGINIIKKVLSAPIRRLVKNAGLESAVIIDYLIKQNDKEL
IYNVEAMNYANAFTAGVIDPAKVVRIAFETAVSVASVLITTESMIVDVPSKENASSPMGAGEMSGMGGF
>Mature_548_residues
TNVVVSGEQLQEAFREVAAIVDSTVAITAGPRGKTVGINKPYGAPEITKDGYKVMKGIKPEKPLNAAIASIFAQSCSQCN
DKVGDGTTTCSILTSNMIMEASKSIAAGNDRVGIKNGIQKAKDVILKEIASMSRTISLEKIDEVAQVAIISANGDKDIGN
SIADSVKKVGKEGVITVEESKGSKELEVELTTGMQFDRGYLSPYFITNNEKMIVELDNPYLLITEKKLNIIQPLLPVLEA
IVKSGKPLVIIAEDIEGEALSTLVINKLRGGLKVAAVKAPGFGDRRKEMLEDIATLTGAKYVIKDELGIKMEDLTLDDLG
TAKNVKITKDNTTVVSENSDSDSVKARIEQIKSQIETSTSDYDKEKLRERLAKLSGGVAVLKVGGATEVEVKERRDRVED
ALHATRAAIEEGIVPGGGVALLYASSVLDKLKGASDEEQIGINIIKKVLSAPIRRLVKNAGLESAVIIDYLIKQNDKELI
YNVEAMNYANAFTAGVIDPAKVVRIAFETAVSVASVLITTESMIVDVPSKENASSPMGAGEMSGMGGF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=507, Percent_Identity=45.7593688362919, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI31542947, Length=507, Percent_Identity=45.7593688362919, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI1790586, Length=520, Percent_Identity=49.6153846153846, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17555558, Length=507, Percent_Identity=47.9289940828402, Blast_Score=455, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=53.3333333333333, Blast_Score=217, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323288, Length=505, Percent_Identity=45.9405940594059, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322350, Length=561, Percent_Identity=23.8859180035651, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24641193, Length=514, Percent_Identity=44.3579766536965, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24641191, Length=514, Percent_Identity=44.3579766536965, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45550936, Length=513, Percent_Identity=43.4697855750487, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45550132, Length=513, Percent_Identity=43.4697855750487, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45550935, Length=513, Percent_Identity=43.4697855750487, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17864606, Length=525, Percent_Identity=39.2380952380952, Blast_Score=354, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24584129, Length=515, Percent_Identity=33.3980582524272, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI19921262, Length=515, Percent_Identity=33.3980582524272, Blast_Score=259, Evalue=4e-69,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58648; Mature: 58517

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVVVSGEQLQEAFREVAAIVDSTVAITAGPRGKTVGINKPYGAPEITKDGYKVMKGIK
CCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHCCC
PEKPLNAAIASIFAQSCSQCNDKVGDGTTTCSILTSNMIMEASKSIAAGNDRVGIKNGIQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KAKDVILKEIASMSRTISLEKIDEVAQVAIISANGDKDIGNSIADSVKKVGKEGVITVEE
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
SKGSKELEVELTTGMQFDRGYLSPYFITNNEKMIVELDNPYLLITEKKLNIIQPLLPVLE
CCCCCEEEEEEECCCEECCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHHHHHHHHHH
AIVKSGKPLVIIAEDIEGEALSTLVINKLRGGLKVAAVKAPGFGDRRKEMLEDIATLTGA
HHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
KYVIKDELGIKMEDLTLDDLGTAKNVKITKDNTTVVSENSDSDSVKARIEQIKSQIETST
CEEEECCCCCEEECCEECCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
SDYDKEKLRERLAKLSGGVAVLKVGGATEVEVKERRDRVEDALHATRAAIEEGIVPGGGV
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ALLYASSVLDKLKGASDEEQIGINIIKKVLSAPIRRLVKNAGLESAVIIDYLIKQNDKEL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHE
IYNVEAMNYANAFTAGVIDPAKVVRIAFETAVSVASVLITTESMIVDVPSKENASSPMGA
EEEEHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCCCCCCC
GEMSGMGGF
CCCCCCCCC
>Mature Secondary Structure 
TNVVVSGEQLQEAFREVAAIVDSTVAITAGPRGKTVGINKPYGAPEITKDGYKVMKGIK
CCEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHCCC
PEKPLNAAIASIFAQSCSQCNDKVGDGTTTCSILTSNMIMEASKSIAAGNDRVGIKNGIQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KAKDVILKEIASMSRTISLEKIDEVAQVAIISANGDKDIGNSIADSVKKVGKEGVITVEE
HHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
SKGSKELEVELTTGMQFDRGYLSPYFITNNEKMIVELDNPYLLITEKKLNIIQPLLPVLE
CCCCCEEEEEEECCCEECCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHHHHHHHHHH
AIVKSGKPLVIIAEDIEGEALSTLVINKLRGGLKVAAVKAPGFGDRRKEMLEDIATLTGA
HHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCC
KYVIKDELGIKMEDLTLDDLGTAKNVKITKDNTTVVSENSDSDSVKARIEQIKSQIETST
CEEEECCCCCEEECCEECCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
SDYDKEKLRERLAKLSGGVAVLKVGGATEVEVKERRDRVEDALHATRAAIEEGIVPGGGV
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ALLYASSVLDKLKGASDEEQIGINIIKKVLSAPIRRLVKNAGLESAVIIDYLIKQNDKEL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHE
IYNVEAMNYANAFTAGVIDPAKVVRIAFETAVSVASVLITTESMIVDVPSKENASSPMGA
EEEEHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCCCCCCC
GEMSGMGGF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA