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The map label for this gene is hemC

Identifier: 225630165

GI number: 225630165

Start: 380037

End: 380915

Strand: Reverse

Name: hemC

Synonym: WRi_003560

Alternate gene names: 225630165

Gene position: 380915-380037 (Counterclockwise)

Preceding gene: 225630169

Following gene: 225630164

Centisome position: 26.34

GC content: 35.49

Gene sequence:

>879_bases
ATGCTAGTCAAAATAGGTACCAGAGGAAGCAAGCTTGCAGTTGTCCAGGCTTTGGAAGCAAAACAAAAGTTGCTGGACTC
TTTTCCTAACTTATCTATTGAAATTGTTAAGATAAAAACTTCTGGAGATAAGTATGCAAACGCAAATCTTGCTGAAATAG
GAGGCAAAGGATTATTCATTAAAGAAATTGAGGCTGAATTGCTCGAAAATAATATAGACATGGCAGTTCACTCGCTAAAG
GATGTGCCTGCATTTTTCTCATGGGGTTTAACAATTCCTTGTGTTTTAGAAAGGCTAAGTCCATGCGATGCGTTTATTTC
TCATAAACACAATAGCCTTGAGTCTTTGCCACAACAGGCTACTATTGCCACTTCTGCAATAAGAAGAAAAGTTCAGTTAC
TAAATTTTAGACCAGATTTAAATATAGTACCGCTACGTGGAAATGTAACAACTAGATTGCAAAATCAAAGTTTTGATGGA
ATAATTTTAGCTGAAGCAGGACTCATAAGATTAGAAAAACATCACTTAATTACAGAGGTATTGCCACCAAAAGTTATGTT
AAGTGCAGTGGGACAGGGAGCGATTTGTATTCAATGTAGAAGGAATGATGTAAAAATTATCGATCTTTTAGAGAAAATTA
ATAATAATATGTCTTTTATAGGAGTGAAATCAGAGCGCAGTTTTATGAAGACAGTGAATGGTTCATGTTTTACACCACTT
GCAGCTTTGGCTGAATATGTAAGCGAAAATATGCTATACCTTCGTTGTATGTTGGCAGATGGAAAAAATATATACTTCAC
TGAGCGCACTTCATTTATAGAAGATGCGGAGAAAATGGGTATGGATGCAGGGTTAGAGTTGAAATCAAAATGCTTATAA

Upstream 100 bases:

>100_bases
GCTTTTGGTTTTAAGTTAAAGTACAATAAATGCAAAGGTTTATATAAACTGGATCCAAGTAGTTAGCTACTTGAATGACA
TCAGACAAACAATCGTACAC

Downstream 100 bases:

>100_bases
GCTTACCTAAAGTACGTGGAATCTATCGTTATGACATCTTAATGTCTAAAGCAACGTGGTTAAATGTCGGTGGACGAGCT
GATATATTATTTAAACCACG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MLVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLK
DVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDG
IILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKIIDLLEKINNNMSFIGVKSERSFMKTVNGSCFTPL
AALAEYVSENMLYLRCMLADGKNIYFTERTSFIEDAEKMGMDAGLELKSKCL

Sequences:

>Translated_292_residues
MLVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLK
DVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDG
IILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKIIDLLEKINNNMSFIGVKSERSFMKTVNGSCFTPL
AALAEYVSENMLYLRCMLADGKNIYFTERTSFIEDAEKMGMDAGLELKSKCL
>Mature_292_residues
MLVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFIKEIEAELLENNIDMAVHSLK
DVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQATIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDG
IILAEAGLIRLEKHHLITEVLPPKVMLSAVGQGAICIQCRRNDVKIIDLLEKINNNMSFIGVKSERSFMKTVNGSCFTPL
AALAEYVSENMLYLRCMLADGKNIYFTERTSFIEDAEKMGMDAGLELKSKCL

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=260, Percent_Identity=36.9230769230769, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI20149500, Length=260, Percent_Identity=36.9230769230769, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI48994974, Length=291, Percent_Identity=43.6426116838488, Blast_Score=234, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6319996, Length=288, Percent_Identity=32.9861111111111, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20130425, Length=258, Percent_Identity=36.4341085271318, Blast_Score=174, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_WOLWR (C0R2M3)

Other databases:

- EMBL:   CP001391
- RefSeq:   YP_002726956.1
- EnsemblBacteria:   EBWOLT00000002146
- GeneID:   7669094
- GenomeReviews:   CP001391_GR
- KEGG:   wri:WRi_003560
- GeneTree:   EBGT00050000030828
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32350; Mature: 32350

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFI
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHCCCCEEH
KEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQA
HHHHHHHHHCCHHHHHHHHHHCHHHHHCCCHHHHHHHHCCHHHHHHHCCCCHHHHCCHHH
TIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEV
HHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCEEEEHHHHHHHHH
LPPKVMLSAVGQGAICIQCRRNDVKIIDLLEKINNNMSFIGVKSERSFMKTVNGSCFTPL
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCHH
AALAEYVSENMLYLRCMLADGKNIYFTERTSFIEDAEKMGMDAGLELKSKCL
HHHHHHHHCCEEEEEEEEECCCEEEEECCHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MLVKIGTRGSKLAVVQALEAKQKLLDSFPNLSIEIVKIKTSGDKYANANLAEIGGKGLFI
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHCCCCEEH
KEIEAELLENNIDMAVHSLKDVPAFFSWGLTIPCVLERLSPCDAFISHKHNSLESLPQQA
HHHHHHHHHCCHHHHHHHHHHCHHHHHCCCHHHHHHHHCCHHHHHHHCCCCHHHHCCHHH
TIATSAIRRKVQLLNFRPDLNIVPLRGNVTTRLQNQSFDGIILAEAGLIRLEKHHLITEV
HHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCEEEEECCCEEEEHHHHHHHHH
LPPKVMLSAVGQGAICIQCRRNDVKIIDLLEKINNNMSFIGVKSERSFMKTVNGSCFTPL
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCHH
AALAEYVSENMLYLRCMLADGKNIYFTERTSFIEDAEKMGMDAGLELKSKCL
HHHHHHHHCCEEEEEEEEECCCEEEEECCHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA