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The map label for this gene is dut [H]

Identifier: 225630061

GI number: 225630061

Start: 239749

End: 240210

Strand: Reverse

Name: dut [H]

Synonym: WRi_002310

Alternate gene names: 225630061

Gene position: 240210-239749 (Counterclockwise)

Preceding gene: 225630062

Following gene: 225630060

Centisome position: 16.61

GC content: 41.13

Gene sequence:

>462_bases
GTGCAAAGAAGTAAAATTAAAGTAGAAATAAAAAGATTATCACACGGAGAAGACTTGTCTCTTCCATGTTATGCAACTGT
GCAAAGTGCTGGTATGGATCTTTATGCTGCATTGAATGACTCTGCTGTTTTAAATCCACTTGAGAGATTACTTGTTCCAA
CTGGAATTGTGATTGCAATACCAAACGGTTTTGAGGGGCAAGTACGCCCACGTTCTGGTCTTGCTGCAAAACATGGAATC
ACTGTCTTAAACTCTCCGGGCACTATAGACTCTGATTATCGAGGTGAGGTTAAAATTTGCCTAATTAATCTAAGTAATCA
ACCATACGAAATAAAAAGAGGAGATAGAATCGCACAAATCCTTATTTCTCCGGTATCTCAGGTAATTTGGGATGACAGAG
AAGAATTCTGCGCAGAAGAAACCGGTCGCAATGCAGGGGGCTTTGGCTCAAGCGGCAGGTAG

Upstream 100 bases:

>100_bases
GAATTTTTATGGCTGCGCTGGAATGACATCATTTACATTTATCACTTTTGCAGATACAATTTACATGCTATAATTGAGTC
TTCATAGATGGTAAATTGGA

Downstream 100 bases:

>100_bases
CTTACGAAAAAGCTTGACTATGACAGTTCTTTTATACACAATTTAAGCATATTAATATTTTATTAAATGAAGTCGTTTAT
GCTAACTTTCAAAAATCATT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MQRSKIKVEIKRLSHGEDLSLPCYATVQSAGMDLYAALNDSAVLNPLERLLVPTGIVIAIPNGFEGQVRPRSGLAAKHGI
TVLNSPGTIDSDYRGEVKICLINLSNQPYEIKRGDRIAQILISPVSQVIWDDREEFCAEETGRNAGGFGSSGR

Sequences:

>Translated_153_residues
MQRSKIKVEIKRLSHGEDLSLPCYATVQSAGMDLYAALNDSAVLNPLERLLVPTGIVIAIPNGFEGQVRPRSGLAAKHGI
TVLNSPGTIDSDYRGEVKICLINLSNQPYEIKRGDRIAQILISPVSQVIWDDREEFCAEETGRNAGGFGSSGR
>Mature_153_residues
MQRSKIKVEIKRLSHGEDLSLPCYATVQSAGMDLYAALNDSAVLNPLERLLVPTGIVIAIPNGFEGQVRPRSGLAAKHGI
TVLNSPGTIDSDYRGEVKICLINLSNQPYEIKRGDRIAQILISPVSQVIWDDREEFCAEETGRNAGGFGSSGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=149, Percent_Identity=41.6107382550336, Blast_Score=109, Evalue=9e-25,
Organism=Homo sapiens, GI4503423, Length=149, Percent_Identity=41.6107382550336, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI70906441, Length=149, Percent_Identity=41.6107382550336, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=45.3333333333333, Blast_Score=116, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=153, Percent_Identity=41.8300653594771, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=150, Percent_Identity=35.3333333333333, Blast_Score=94, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19921126, Length=154, Percent_Identity=34.4155844155844, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=36.8421052631579, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16623; Mature: 16623

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSKIKVEIKRLSHGEDLSLPCYATVQSAGMDLYAALNDSAVLNPLERLLVPTGIVIAI
CCCCCEEEEEEECCCCCCCCCCHHEEHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEC
PNGFEGQVRPRSGLAAKHGITVLNSPGTIDSDYRGEVKICLINLSNQPYEIKRGDRIAQI
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHHH
LISPVSQVIWDDREEFCAEETGRNAGGFGSSGR
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQRSKIKVEIKRLSHGEDLSLPCYATVQSAGMDLYAALNDSAVLNPLERLLVPTGIVIAI
CCCCCEEEEEEECCCCCCCCCCHHEEHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEC
PNGFEGQVRPRSGLAAKHGITVLNSPGTIDSDYRGEVKICLINLSNQPYEIKRGDRIAQI
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHHH
LISPVSQVIWDDREEFCAEETGRNAGGFGSSGR
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA