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The map label for this gene is ribH

Identifier: 225630057

GI number: 225630057

Start: 236242

End: 236670

Strand: Direct

Name: ribH

Synonym: WRi_002270

Alternate gene names: 225630057

Gene position: 236242-236670 (Clockwise)

Preceding gene: 225630056

Following gene: 225630058

Centisome position: 16.34

GC content: 35.9

Gene sequence:

>429_bases
ATGTCGAGAATACTAATAGTTAATTCGATTTATTATACTGAAATAGCGAACCTTTTGCTTGAAGGTGCAATTGATAAATT
AAAAGGCAGTAATGCCAGTTACGATGTAGTTGAGGTTCCTGGTACATTTGAGATACCAGCTACAATTCTTTTTGCAGTCA
AGAGTGGGCATACTAATTATGATGGTTATTTAGCTCTTGGGTGCGTAATTCGCGGTGAAACCGATCATTATCAATATGTT
TGTAAAGGAGTAATTGAAGGCTTAAATGAGGTTGTTATGCATTATGCAATACCCCTTGGTATGGGTGTAATTACCGCTGA
CAGCAAAGATAAAGCACTAGTTAGAGCTGATAAAAACAAAAAGAACGTTGGTGGCCATGCAGCCTCAACTGTTTTGCGTA
TGATAGATTTACACAATAAATTAAAATAA

Upstream 100 bases:

>100_bases
TTTCCTGCCGGCCTGGTAATATATTGGATATTTAGTAATATTATAACTCTAATTCAACAGTCATTAATAAAGTTGTTTTT
AACAAAAAAAGTGGTAGTAA

Downstream 100 bases:

>100_bases
TGGAGGAAAAACCAGTAGATAAAAAGTGGCGCATAAAAAGAAGCACGGCAAGATTTCTTGCTGTACAAATTGCTTATTCA
AATATTTTCGTAGGTTACAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MSRILIVNSIYYTEIANLLLEGAIDKLKGSNASYDVVEVPGTFEIPATILFAVKSGHTNYDGYLALGCVIRGETDHYQYV
CKGVIEGLNEVVMHYAIPLGMGVITADSKDKALVRADKNKKNVGGHAASTVLRMIDLHNKLK

Sequences:

>Translated_142_residues
MSRILIVNSIYYTEIANLLLEGAIDKLKGSNASYDVVEVPGTFEIPATILFAVKSGHTNYDGYLALGCVIRGETDHYQYV
CKGVIEGLNEVVMHYAIPLGMGVITADSKDKALVRADKNKKNVGGHAASTVLRMIDLHNKLK
>Mature_141_residues
SRILIVNSIYYTEIANLLLEGAIDKLKGSNASYDVVEVPGTFEIPATILFAVKSGHTNYDGYLALGCVIRGETDHYQYVC
KGVIEGLNEVVMHYAIPLGMGVITADSKDKALVRADKNKKNVGGHAASTVLRMIDLHNKLK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=145, Percent_Identity=39.3103448275862, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_WOLPM (P61729)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966048.1
- ProteinModelPortal:   P61729
- SMR:   P61729
- EnsemblBacteria:   EBWOLT00000003944
- GeneID:   2737922
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0238
- TIGR:   WD_0238
- GeneTree:   EBGT00050000031345
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- PhylomeDB:   P61729
- ProtClustDB:   PRK00061
- BioCyc:   WPIP955:WD_0238-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 15468; Mature: 15337

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILIVNSIYYTEIANLLLEGAIDKLKGSNASYDVVEVPGTFEIPATILFAVKSGHTNY
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCC
DGYLALGCVIRGETDHYQYVCKGVIEGLNEVVMHYAIPLGMGVITADSKDKALVRADKNK
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCC
KNVGGHAASTVLRMIDLHNKLK
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRILIVNSIYYTEIANLLLEGAIDKLKGSNASYDVVEVPGTFEIPATILFAVKSGHTNY
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCC
DGYLALGCVIRGETDHYQYVCKGVIEGLNEVVMHYAIPLGMGVITADSKDKALVRADKNK
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCC
KNVGGHAASTVLRMIDLHNKLK
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA