Click here to switch to the map view.

The map label for this gene is nrdF [H]

Identifier: 225630036

GI number: 225630036

Start: 204804

End: 205793

Strand: Reverse

Name: nrdF [H]

Synonym: WRi_002040

Alternate gene names: 225630036

Gene position: 205793-204804 (Counterclockwise)

Preceding gene: 225630038

Following gene: 225630035

Centisome position: 14.23

GC content: 36.97

Gene sequence:

>990_bases
ATGTCATTGTTAGAAGCAGATCCAATATACAAACCTTTTAATTACCCTTGGGCGTATGATGCGTGGCTGCAGCAACAAAG
AATCCATTGGATACCTGAGGAAGTTCCGCTTGCTGATGACGTAAAGGATTGGAAAACTAAACTTTCAAGTGTAGAAAAAA
ATCTACTAACCCAGATCTTTAGATTTTTTACTCAGGCAGACATTGAAGTAAATAACTGCTACATGAGGCATTATTCAAAT
ATATTTAAGCCAACGGAAATATGCATGATGCTTGCAAGCTTTTCCAATATGGAAACTATACACATTGCAGCTTATTCTTA
TCTTTTAGACACAATTGGCATGCCAGAAAGCGAGTATCAAGCATTCTTAAAGTATGATGCTATGAGAAAGAAGTATGAAT
ACATGTTAGAATTCGAGGAAAGCAAAAAACACGATAAAAAACATGTAGCTAAAACTCTAGCGGTGTTTGGTGCATTTACC
GAGGGGTTGCAGTTATTCGCATCATTTGCAATTTTGCTCAATTTTCAACGCTTTGGAAAAATGAAAGGCATGGGACAAAT
AATTGCCTGGTCAGCCCGTGATGAAACTTTGCACACCAATTCAATTATCATGTTATTTAATACATTTATTAAAGAGAATA
ATGAAATTTGGGACAACGAGTTTAAAGAGGAATTATATTCTGCATGTCGCACCATCGTTGAACTTGAGGACGAATTCATA
AAGCTTGCCTTTGATTTAGGAGATGTTGAAGGACTATCCGCAGAAGAAGTGCGCAATTACATACGCTACGTAGCAAACAG
ACGGTTAATGCAATTAGGTCTTGAGTCCATATATGATGTCAATGATAATCCTATTCCGTGGCTTGATGAAATACTAAATG
GCGTTGAACATACGAATTTCTTTGAAAATAGAGTAACAGAGTATAGCCGCGCAGCAACTCAAGGCACGTGGGAAGAAGCT
TTTACTGAAAATGACACGAATAATAAGTGA

Upstream 100 bases:

>100_bases
CACTAATAAATTATCTGCCGTCAAATTTCAAAAAAGTTAAATTGACTGATTCTCAATATCACTTAATATAATATTTACTT
TAGAAAATAATAGGTGTCTT

Downstream 100 bases:

>100_bases
ATGCTAATTTCACTCTTTTTTGTCATTCCACTTTCATTACACCATTTGCTGTGCAGTTTAGACAGTTTTGCTTTACTTAA
CAACTAACTTACCATATATA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSSVEKNLLTQIFRFFTQADIEVNNCYMRHYSN
IFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKHDKKHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIIMLFNTFIKENNEIWDNEFKEELYSACRTIVELEDEFI
KLAFDLGDVEGLSAEEVRNYIRYVANRRLMQLGLESIYDVNDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEA
FTENDTNNK

Sequences:

>Translated_329_residues
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSSVEKNLLTQIFRFFTQADIEVNNCYMRHYSN
IFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKHDKKHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIIMLFNTFIKENNEIWDNEFKEELYSACRTIVELEDEFI
KLAFDLGDVEGLSAEEVRNYIRYVANRRLMQLGLESIYDVNDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEA
FTENDTNNK
>Mature_328_residues
SLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSSVEKNLLTQIFRFFTQADIEVNNCYMRHYSNI
FKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKHDKKHVAKTLAVFGAFTE
GLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIIMLFNTFIKENNEIWDNEFKEELYSACRTIVELEDEFIK
LAFDLGDVEGLSAEEVRNYIRYVANRRLMQLGLESIYDVNDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEAF
TENDTNNK

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=317, Percent_Identity=29.0220820189274, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI289177074, Length=317, Percent_Identity=28.7066246056782, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4557845, Length=317, Percent_Identity=27.4447949526814, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI260064013, Length=317, Percent_Identity=27.4447949526814, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI289177076, Length=296, Percent_Identity=28.7162162162162, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789031, Length=287, Percent_Identity=25.4355400696864, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788567, Length=298, Percent_Identity=22.4832214765101, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=289, Percent_Identity=28.719723183391, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322434, Length=329, Percent_Identity=27.6595744680851, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18079279, Length=319, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38769; Mature: 38638

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSSVEKNLLTQIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVNNCYMRHYSNIFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQ
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
AFLKYDAMRKKYEYMLEFEESKKHDKKHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGMGQIIAWSARDETLHTNSIIMLFNTFIKENNEIWDNEFKEELYSACRTIVELEDEFI
HCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLAFDLGDVEGLSAEEVRNYIRYVANRRLMQLGLESIYDVNDNPIPWLDEILNGVEHTNF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FENRVTEYSRAATQGTWEEAFTENDTNNK
HHHHHHHHHHHHCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSSVEKNLLTQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVNNCYMRHYSNIFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQ
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
AFLKYDAMRKKYEYMLEFEESKKHDKKHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGMGQIIAWSARDETLHTNSIIMLFNTFIKENNEIWDNEFKEELYSACRTIVELEDEFI
HCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLAFDLGDVEGLSAEEVRNYIRYVANRRLMQLGLESIYDVNDNPIPWLDEILNGVEHTNF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FENRVTEYSRAATQGTWEEAFTENDTNNK
HHHHHHHHHHHHCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]