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The map label for this gene is nrdA [H]

Identifier: 225630021

GI number: 225630021

Start: 185274

End: 187058

Strand: Reverse

Name: nrdA [H]

Synonym: WRi_001830

Alternate gene names: 225630021

Gene position: 187058-185274 (Counterclockwise)

Preceding gene: 225630022

Following gene: 225630020

Centisome position: 12.94

GC content: 36.92

Gene sequence:

>1785_bases
ATGAATAACATTACCATTAATTACGCAAAGGACAGCAAATTAACCGACTTTGGAAAAGCAGTTCTATCAGACAGGTATTT
AATAGAAAATGAAAGTTATCAAGACCTCTTTGCACGTATTGCTAATTACTATTCTGATAATAAAGAACATGCGCAGCGTC
TTTATGACTACATGAGCAACTTGTGGTTCATGCCTTCAACACCAATACTCAGCAATGGTGGCACTAAGAGAGGGTTACCT
ATCTCTTGCTTTCTTAATGAAACCGAAGACAGCTTGCAGGGAATAGTTGATCTATGGAATGAAAATGTTTGGCTTGCTGC
ACGTGGAGGAGGCATAGGTAGTTATTGGGGAAATTTACGCTCAATAGGCGAAAGCGTAAAGGGTAGCGGCAAAACATCAG
GAATTGTTCCATTTATTGTAGTGCAAAATGCTCTAACGCTTGCAATTAGTCAGGGATCCCTAAGGCGAGGAAGTTCAGCA
GTCTACCTTCCTGTATCTCATCCGGAGATAGAAGAGTTTCTAGATTTACGCAAGCCAACAGGCGGTGACCCAAATCGCAA
AGCGTTAAATATACATCATGCTGTAATAGTAACAGATAAATTTATGCAGGCTGTTGAGAATGATCAAGAATGGAATTTAA
TAAGCCCTCACAACAATAAGGTTATTTCAACTGTAAAAGCGCGGGATATATGGATCAAAATATTAACAGCAAGAGTTGAA
ACTGGAGAACCTTACATTATTTTCCTTGATGCAACAAATAACAATAAGCCAGAATCTTACAAAAAGCTCAACTTAGACAT
CAAGATGTCAAATCTATGCAGTGAAATAACTTTAACCACAGGTTATGATCACCTGAATAAGTCACGCACTGCTGTGTGTT
GTCTATCATCCGTAAACCTTGAGTACTACGAAGAATGGAAAGACAATAAACTCTTCATAGAAGATATAATGCGCTTTCTT
GATAATGTATTGGAAGATTTCATAAATAAAGCACCGAATGAAATGCAGCGAGCAAAATATTCTGCAGCCAGAGAACGCAG
TATTGGTCTTGGTGTGATGGGCTTTCATTCATTTTTACAAAGCAAAATGGTTCCTTTTGAATCAGTAACAGCACAACAAT
GGAATAAAAAAATATTTAAGTATTTACGCGAGCAAGCAGATATAGTTTCTAAAAAATTAGCAGAAGAAAAGGGAGCATGT
TCTGATGCCAAAGAAGTTGATCTAATGGAAAGGTTTACACACAAACTCGCTATTGCTCCTACTGCTTCGATCTCCATTAT
TGCAGGTAATACCTCTCCTGGAATAGAACCATATGCAGCAAACGTATTCATACAAAAGACACTTACAGGTTCATTTGTAG
TGCGAAATAAATTCTTGCAAAAACTATTAGCAGAAAAAAATCAAGACAATGATAAAATATGGTCTTCAATTTCAACAAAC
GAGGGTTCCGTTCAGCATTTAGATTTTCTTAGTGAGCATGAAAAATTGACATTTAAAACAGCGTACGAGCTTGACCAAAG
ATGGATTATAGAACATGCAAGTGATAGAACTTCGTATATTTGTCAATCTCAATCAGTAAATCTGTTCTTACCTGCCAACG
TACATAAACGTTACTTGCACAAAATACACATGCTTGCTTGGAAAAAAGGATTGAAGAGCTTATATTACTGCAGATCACAA
TCAATGCAGAGAGCCGATAAGGTCTCGCATGACATATTCAAAAAAAGTGAAATATTGCAACAAAAAACAGATATTGACTA
CGATGAATGTCTGTCATGCCAATAG

Upstream 100 bases:

>100_bases
GCATTACAGCATCAACAAGAAAATCCGAAAAACCTAACTTAAAAATCGCTCAAAATGACTTTTAAGTATCTATATATAAT
AAAAGAATAAAAGGAACTTT

Downstream 100 bases:

>100_bases
CGGCCTTACTCAAAAAAAGTAGAGAGAAAGTTAAGCAAATGGGTAAATAGACTTCTTGCATAACCATATAACGCACTTTT
ATTGGGAATAGAAACGAAAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNNITINYAKDSKLTDFGKAVLSDRYLIENESYQDLFARIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVSHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVENDQEWNLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLNKSRTAVCCLSSVNLEYYEEWKDNKLFIEDIMRFL
DNVLEDFINKAPNEMQRAKYSAARERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKIFKYLREQADIVSKKLAEEKGAC
SDAKEVDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDKIWSSISTN
EGSVQHLDFLSEHEKLTFKTAYELDQRWIIEHASDRTSYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDIDYDECLSCQ

Sequences:

>Translated_594_residues
MNNITINYAKDSKLTDFGKAVLSDRYLIENESYQDLFARIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVSHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVENDQEWNLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLNKSRTAVCCLSSVNLEYYEEWKDNKLFIEDIMRFL
DNVLEDFINKAPNEMQRAKYSAARERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKIFKYLREQADIVSKKLAEEKGAC
SDAKEVDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDKIWSSISTN
EGSVQHLDFLSEHEKLTFKTAYELDQRWIIEHASDRTSYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDIDYDECLSCQ
>Mature_594_residues
MNNITINYAKDSKLTDFGKAVLSDRYLIENESYQDLFARIANYYSDNKEHAQRLYDYMSNLWFMPSTPILSNGGTKRGLP
ISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLRSIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSA
VYLPVSHPEIEEFLDLRKPTGGDPNRKALNIHHAVIVTDKFMQAVENDQEWNLISPHNNKVISTVKARDIWIKILTARVE
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLNKSRTAVCCLSSVNLEYYEEWKDNKLFIEDIMRFL
DNVLEDFINKAPNEMQRAKYSAARERSIGLGVMGFHSFLQSKMVPFESVTAQQWNKKIFKYLREQADIVSKKLAEEKGAC
SDAKEVDLMERFTHKLAIAPTASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDKIWSSISTN
EGSVQHLDFLSEHEKLTFKTAYELDQRWIIEHASDRTSYICQSQSVNLFLPANVHKRYLHKIHMLAWKKGLKSLYYCRSQ
SMQRADKVSHDIFKKSEILQQKTDIDYDECLSCQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=627, Percent_Identity=30.4625199362041, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1789030, Length=598, Percent_Identity=26.5886287625418, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI2367133, Length=635, Percent_Identity=22.992125984252, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555242, Length=661, Percent_Identity=30.8623298033283, Blast_Score=256, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6320914, Length=637, Percent_Identity=31.0832025117739, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322123, Length=638, Percent_Identity=30.2507836990596, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17352481, Length=627, Percent_Identity=29.3460925039872, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 67666; Mature: 67666

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNITINYAKDSKLTDFGKAVLSDRYLIENESYQDLFARIANYYSDNKEHAQRLYDYMSN
CCCEEEEECCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LWFMPSTPILSNGGTKRGLPISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLR
CEECCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
SIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSAVYLPVSHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEEHHCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCC
GGDPNRKALNIHHAVIVTDKFMQAVENDQEWNLISPHNNKVISTVKARDIWIKILTARVE
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCEECCCCCCEEEEEHHHHHEEEEEEEEEC
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLNKSRTAVCCLSSVNL
CCCCEEEEEECCCCCCCCCHHEEEEEEEHHHHHHHHEEECCHHHHCCCCCEEEEEECCCH
EYYEEWKDNKLFIEDIMRFLDNVLEDFINKAPNEMQRAKYSAARERSIGLGVMGFHSFLQ
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMVPFESVTAQQWNKKIFKYLREQADIVSKKLAEEKGACSDAKEVDLMERFTHKLAIAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECC
TASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDKIWSSISTN
CCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
EGSVQHLDFLSEHEKLTFKTAYELDQRWIIEHASDRTSYICQSQSVNLFLPANVHKRYLH
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCHHHHHHHH
KIHMLAWKKGLKSLYYCRSQSMQRADKVSHDIFKKSEILQQKTDIDYDECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure
MNNITINYAKDSKLTDFGKAVLSDRYLIENESYQDLFARIANYYSDNKEHAQRLYDYMSN
CCCEEEEECCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
LWFMPSTPILSNGGTKRGLPISCFLNETEDSLQGIVDLWNENVWLAARGGGIGSYWGNLR
CEECCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
SIGESVKGSGKTSGIVPFIVVQNALTLAISQGSLRRGSSAVYLPVSHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEEHHCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCC
GGDPNRKALNIHHAVIVTDKFMQAVENDQEWNLISPHNNKVISTVKARDIWIKILTARVE
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCCCEECCCCCCEEEEEHHHHHEEEEEEEEEC
TGEPYIIFLDATNNNKPESYKKLNLDIKMSNLCSEITLTTGYDHLNKSRTAVCCLSSVNL
CCCCEEEEEECCCCCCCCCHHEEEEEEEHHHHHHHHEEECCHHHHCCCCCEEEEEECCCH
EYYEEWKDNKLFIEDIMRFLDNVLEDFINKAPNEMQRAKYSAARERSIGLGVMGFHSFLQ
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMVPFESVTAQQWNKKIFKYLREQADIVSKKLAEEKGACSDAKEVDLMERFTHKLAIAP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECC
TASISIIAGNTSPGIEPYAANVFIQKTLTGSFVVRNKFLQKLLAEKNQDNDKIWSSISTN
CCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
EGSVQHLDFLSEHEKLTFKTAYELDQRWIIEHASDRTSYICQSQSVNLFLPANVHKRYLH
CCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCHHHHHHHH
KIHMLAWKKGLKSLYYCRSQSMQRADKVSHDIFKKSEILQQKTDIDYDECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]