Click here to switch to the map view.

The map label for this gene is rnd [H]

Identifier: 225630014

GI number: 225630014

Start: 174762

End: 175940

Strand: Reverse

Name: rnd [H]

Synonym: WRi_001760

Alternate gene names: 225630014

Gene position: 175940-174762 (Counterclockwise)

Preceding gene: 225630016

Following gene: 225630013

Centisome position: 12.17

GC content: 32.15

Gene sequence:

>1179_bases
ATGTTAATTAATACAACGTCGGAGCTGGAGGACATATGTGAGGGGTTGATAGCAAAAAAGCCAAAATTTATAGCAGTTGA
CACGGAGTTCATTAGAAATAATTTAATCTACTACCCAAAATTATCGTTAATTCAAATTTCTTACGGAGAGAAGAGTTTTA
TTGTAGACGCATTAGTGCCAGAAATTGATTTATCATTCATTAAGAAAATAATGCTAAATCAGGGGATAACCAAAGTGTTT
CATAGCTGCCGGCAGGATATAGAATCCTTACTCACTGTGTTTAAATGTATTCCCACTCCCATTTTTGATACCCAAGTTGC
TGCTATGTTTTGTCATTATTATCATGACTTTATTGGTTACTCAAAAGTAGTGGAGCAATATCAAGGAATAGCACTGGATA
AAATTAAAGCTAAAAATTCAGATTGGTTAAGGCGTCCGTTGTCCGAAGATCAGTTAGATTATGCAGTAAACGACGTGGTA
CACCTATATGACCTATACCAAATATTGTGCAATAAACTTGAAGAAAATAATAGGATGGGTTGGTTTCAAGAAGAGATGGA
ATCAATAGTTGATATAAATAAGTATTTACATAGCCCAAAAGATGCATGGAAGAGGATTAAATTTAATTATGAAGTAAATC
CAAGATTGATACTAACTGTTAAAGCAGTTAGTGAGTGGCAAGAAACCTTAGCACAGCGCTATAATATGAACCGTAATAAA
ATAATCAATAATGCTGTAATAGCTGATTTTATTGAAAAAAATGTAGAGCATGTTGATGAGATTTTAGATGATCTCAAGAG
GAATACAAAAAATATAAAAGATGCGGATTTACTAGAGTTTGTGAATATTTTTAATGAAAATGAGAGAAATTGGATGCAGC
AAAACAACACCTTATCGAGTAATTATGACAAATCTGTACTTGATATACTCTCAATTATTTTAGATAGTAAATGTAAAGAA
AGTAACATATCAAGGAAATTAGTTTCTTCAAAAGATGAGTTAACCGGGTCAATATCTGGACAGACAGATAAATTATTCAA
GGGATGGAGATATGATTTTTTTGGCAGGTCAGTTGAATCATTTTTGAACACAAGCTCAAAATTTGAGATTTCAGCAGTGA
AATCCGCAAATAATATAACCAAAATTCAGAGTAATTTAGTGGAGAATAACTGTTGCTAA

Upstream 100 bases:

>100_bases
ATATTATAAGTAACTAGCAAGCTCCATTACTAATTTCGTAGTGACTCTAATTGCAAAATTTAATATAAGTAGTAAAATAG
AACCACTGAATAAAAAACAT

Downstream 100 bases:

>100_bases
AGTAACTTAGATTTATCGACAATCGTCATCCCACTACGTGTTAGTGGCTAAGAGATACCGCGGCGGTATGACGGTTAAGT
AGGGTGTCATCCCAGTGCCT

Product: Ribonuclease D

Products: NA

Alternate protein names: RNase D [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MLINTTSELEDICEGLIAKKPKFIAVDTEFIRNNLIYYPKLSLIQISYGEKSFIVDALVPEIDLSFIKKIMLNQGITKVF
HSCRQDIESLLTVFKCIPTPIFDTQVAAMFCHYYHDFIGYSKVVEQYQGIALDKIKAKNSDWLRRPLSEDQLDYAVNDVV
HLYDLYQILCNKLEENNRMGWFQEEMESIVDINKYLHSPKDAWKRIKFNYEVNPRLILTVKAVSEWQETLAQRYNMNRNK
IINNAVIADFIEKNVEHVDEILDDLKRNTKNIKDADLLEFVNIFNENERNWMQQNNTLSSNYDKSVLDILSIILDSKCKE
SNISRKLVSSKDELTGSISGQTDKLFKGWRYDFFGRSVESFLNTSSKFEISAVKSANNITKIQSNLVENNCC

Sequences:

>Translated_392_residues
MLINTTSELEDICEGLIAKKPKFIAVDTEFIRNNLIYYPKLSLIQISYGEKSFIVDALVPEIDLSFIKKIMLNQGITKVF
HSCRQDIESLLTVFKCIPTPIFDTQVAAMFCHYYHDFIGYSKVVEQYQGIALDKIKAKNSDWLRRPLSEDQLDYAVNDVV
HLYDLYQILCNKLEENNRMGWFQEEMESIVDINKYLHSPKDAWKRIKFNYEVNPRLILTVKAVSEWQETLAQRYNMNRNK
IINNAVIADFIEKNVEHVDEILDDLKRNTKNIKDADLLEFVNIFNENERNWMQQNNTLSSNYDKSVLDILSIILDSKCKE
SNISRKLVSSKDELTGSISGQTDKLFKGWRYDFFGRSVESFLNTSSKFEISAVKSANNITKIQSNLVENNCC
>Mature_392_residues
MLINTTSELEDICEGLIAKKPKFIAVDTEFIRNNLIYYPKLSLIQISYGEKSFIVDALVPEIDLSFIKKIMLNQGITKVF
HSCRQDIESLLTVFKCIPTPIFDTQVAAMFCHYYHDFIGYSKVVEQYQGIALDKIKAKNSDWLRRPLSEDQLDYAVNDVV
HLYDLYQILCNKLEENNRMGWFQEEMESIVDINKYLHSPKDAWKRIKFNYEVNPRLILTVKAVSEWQETLAQRYNMNRNK
IINNAVIADFIEKNVEHVDEILDDLKRNTKNIKDADLLEFVNIFNENERNWMQQNNTLSSNYDKSVLDILSIILDSKCKE
SNISRKLVSSKDELTGSISGQTDKLFKGWRYDFFGRSVESFLNTSSKFEISAVKSANNITKIQSNLVENNCC

Specific function: Cleaves multimeric tRNA precursor at the spacer region. Contribute to the 3' maturation of several stable RNAs [H]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=379, Percent_Identity=26.6490765171504, Blast_Score=138, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324574, Length=264, Percent_Identity=27.6515151515151, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR002121
- InterPro:   IPR010997
- InterPro:   IPR006292
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00570 HRDC [H]

EC number: =3.1.13.5 [H]

Molecular weight: Translated: 45582; Mature: 45582

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINTTSELEDICEGLIAKKPKFIAVDTEFIRNNLIYYPKLSLIQISYGEKSFIVDALVP
CCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCEEECCEEEEEEECCCHHHHHHHHHC
EIDLSFIKKIMLNQGITKVFHSCRQDIESLLTVFKCIPTPIFDTQVAAMFCHYYHDFIGY
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SKVVEQYQGIALDKIKAKNSDWLRRPLSEDQLDYAVNDVVHLYDLYQILCNKLEENNRMG
HHHHHHHHCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WFQEEMESIVDINKYLHSPKDAWKRIKFNYEVNPRLILTVKAVSEWQETLAQRYNMNRNK
HHHHHHHHHHHHHHHHCCCHHHHHHEEECEECCCEEEEEHHHHHHHHHHHHHHHCCCHHH
IINNAVIADFIEKNVEHVDEILDDLKRNTKNIKDADLLEFVNIFNENERNWMQQNNTLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NYDKSVLDILSIILDSKCKESNISRKLVSSKDELTGSISGQTDKLFKGWRYDFFGRSVES
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHCCCHHHHHHHHHH
FLNTSSKFEISAVKSANNITKIQSNLVENNCC
HHCCCCCEEEHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLINTTSELEDICEGLIAKKPKFIAVDTEFIRNNLIYYPKLSLIQISYGEKSFIVDALVP
CCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCEEECCEEEEEEECCCHHHHHHHHHC
EIDLSFIKKIMLNQGITKVFHSCRQDIESLLTVFKCIPTPIFDTQVAAMFCHYYHDFIGY
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SKVVEQYQGIALDKIKAKNSDWLRRPLSEDQLDYAVNDVVHLYDLYQILCNKLEENNRMG
HHHHHHHHCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WFQEEMESIVDINKYLHSPKDAWKRIKFNYEVNPRLILTVKAVSEWQETLAQRYNMNRNK
HHHHHHHHHHHHHHHHCCCHHHHHHEEECEECCCEEEEEHHHHHHHHHHHHHHHCCCHHH
IINNAVIADFIEKNVEHVDEILDDLKRNTKNIKDADLLEFVNIFNENERNWMQQNNTLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NYDKSVLDILSIILDSKCKESNISRKLVSSKDELTGSISGQTDKLFKGWRYDFFGRSVES
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHCCCHHHHHHHHHH
FLNTSSKFEISAVKSANNITKIQSNLVENNCC
HHCCCCCEEEHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3041371; 9097040; 9278503; 8107670 [H]