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The map label for this gene is tpiA [H]

Identifier: 225630005

GI number: 225630005

Start: 160267

End: 160995

Strand: Reverse

Name: tpiA [H]

Synonym: WRi_001590

Alternate gene names: 225630005

Gene position: 160995-160267 (Counterclockwise)

Preceding gene: 225630009

Following gene: 225630003

Centisome position: 11.13

GC content: 31.96

Gene sequence:

>729_bases
ATGTCCTTTTTAATAGTAGCAAATTGGAAAATGAATGGAATGCGTTCTTCATTTGTTGACTTTATAGGCAAACTTAACAA
CAAGAGCAACGAAATTACCTCTAAATTAGTAATTTGCCCTCCTTTTACGTCATTTCCAAGCAGTATAGAGTTGAACAATA
ATATTAATATAGGAGCACAGAATTGCCATCATAAAAAGTTCGGTTCTTACACAGGTGAAATTAGCGCAGAAATGTTAAAA
GAACTAGGGTGTACTTACGTAATACTTGGGCATTCTGAAAGGGCCAATGAAAAAGATAGTGAAATAAAACTTAAGTCGGA
AATAGCAATAGAATCAGGCTTACACCCAATTATCTGTGTAGGTGAAAATTCAGAAGATTATAAGAATGAAAAAACAAAAG
AAGTAATAGAATATCAATGTAAAAACCGCTTGCCAACACACGGTGAATATACCGTAGCATATGAGCCAATATGGGCAATA
GGCACAGGTCATGTGCCAAATAATGATGCAATTGCTAAGGTGATAGAGGTAATAAAATTGTGTACTAGTAAAAAACACAT
TATATATGGTGGTTCAGTCAGTTCAGAAAATATAGAAAATTTGTTAAATATTTCAAATTTATCAGGAGTTTTAATTGGCA
GTGCAAGCTTAGATTTCGATCACTTTTATAAAATTATACAACAAGTTGAAAAAAAGTTTTCTCTTATCAATTCTAAAATC
AGCAACTAA

Upstream 100 bases:

>100_bases
AACGCCGGCTTAGCTCAATTGGTAGAGCAACTGACTTGTAATCAGTAGGTTGTCAGTTCAAGTCCGACAGCCGGCACTCT
CGCCCATATAAGTAAAGTTG

Downstream 100 bases:

>100_bases
GAATCCTATGATAAATGTCATCATTCCAGTGTCAAGCATATATAGCTAACCCAAGAAAGATTTCCCTACGTCATACCGCG
ATTCATTCGCGGTATCTCAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSFLIVANWKMNGMRSSFVDFIGKLNNKSNEITSKLVICPPFTSFPSSIELNNNINIGAQNCHHKKFGSYTGEISAEMLK
ELGCTYVILGHSERANEKDSEIKLKSEIAIESGLHPIICVGENSEDYKNEKTKEVIEYQCKNRLPTHGEYTVAYEPIWAI
GTGHVPNNDAIAKVIEVIKLCTSKKHIIYGGSVSSENIENLLNISNLSGVLIGSASLDFDHFYKIIQQVEKKFSLINSKI
SN

Sequences:

>Translated_242_residues
MSFLIVANWKMNGMRSSFVDFIGKLNNKSNEITSKLVICPPFTSFPSSIELNNNINIGAQNCHHKKFGSYTGEISAEMLK
ELGCTYVILGHSERANEKDSEIKLKSEIAIESGLHPIICVGENSEDYKNEKTKEVIEYQCKNRLPTHGEYTVAYEPIWAI
GTGHVPNNDAIAKVIEVIKLCTSKKHIIYGGSVSSENIENLLNISNLSGVLIGSASLDFDHFYKIIQQVEKKFSLINSKI
SN
>Mature_241_residues
SFLIVANWKMNGMRSSFVDFIGKLNNKSNEITSKLVICPPFTSFPSSIELNNNINIGAQNCHHKKFGSYTGEISAEMLKE
LGCTYVILGHSERANEKDSEIKLKSEIAIESGLHPIICVGENSEDYKNEKTKEVIEYQCKNRLPTHGEYTVAYEPIWAIG
TGHVPNNDAIAKVIEVIKLCTSKKHIIYGGSVSSENIENLLNISNLSGVLIGSASLDFDHFYKIIQQVEKKFSLINSKIS
N

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=232, Percent_Identity=34.4827586206897, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI4507645, Length=241, Percent_Identity=34.4398340248963, Blast_Score=132, Evalue=2e-31,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=34.9397590361446, Blast_Score=133, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=35.6846473029046, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320255, Length=233, Percent_Identity=34.7639484978541, Blast_Score=136, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=34.5833333333333, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=34.5833333333333, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28572004, Length=231, Percent_Identity=34.6320346320346, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26946; Mature: 26815

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLIVANWKMNGMRSSFVDFIGKLNNKSNEITSKLVICPPFTSFPSSIELNNNINIGAQ
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCEEECCCCCCCCC
NCHHKKFGSYTGEISAEMLKELGCTYVILGHSERANEKDSEIKLKSEIAIESGLHPIICV
CCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHCCCCEEEEE
GENSEDYKNEKTKEVIEYQCKNRLPTHGEYTVAYEPIWAIGTGHVPNNDAIAKVIEVIKL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHH
CTSKKHIIYGGSVSSENIENLLNISNLSGVLIGSASLDFDHFYKIIQQVEKKFSLINSKI
HCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
SN
CC
>Mature Secondary Structure 
SFLIVANWKMNGMRSSFVDFIGKLNNKSNEITSKLVICPPFTSFPSSIELNNNINIGAQ
EEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCEEECCCCCCCCC
NCHHKKFGSYTGEISAEMLKELGCTYVILGHSERANEKDSEIKLKSEIAIESGLHPIICV
CCCCHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEHHHHHHHCCCCEEEEE
GENSEDYKNEKTKEVIEYQCKNRLPTHGEYTVAYEPIWAIGTGHVPNNDAIAKVIEVIKL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHH
CTSKKHIIYGGSVSSENIENLLNISNLSGVLIGSASLDFDHFYKIIQQVEKKFSLINSKI
HCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
SN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA