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The map label for this gene is fabI [H]

Identifier: 225630009

GI number: 225630009

Start: 166228

End: 167013

Strand: Reverse

Name: fabI [H]

Synonym: WRi_001670

Alternate gene names: 225630009

Gene position: 167013-166228 (Counterclockwise)

Preceding gene: 225630012

Following gene: 225630005

Centisome position: 11.55

GC content: 36.13

Gene sequence:

>786_bases
ATGACGACAAGTCTATTACAAGGCAAAAAGGGATTAATAACCGGAATAATAAATAAGAGATCAATAGCATACGGTATAGC
AAAAACTCTCTCAGAGCACGGAGCAGAACTTGCAATCACCTACCAGAATGAAATAATAAAAGAAAAACTATCACCAATAG
CAGATGAACTAAATGTGGAGCTAATATTACACTGCGACGTTTCAAATGAAAAAATCATAGATAACGCTTTCGAAATAATA
GAGAAAAAATGGGGTACTCTTGACTTTTTGGTGCATGCAATAGCATTCTCCGATAAAAATGAGTTAGATGGTAAATATGT
CAATACTTCACTGGATAACTTCCTCAATGCAATGCATATATCGTGCTATTCTTTTACTGCTTTAGCGCAAAGGGCTGAAA
AAATGATGTCAAATGGTGGTAGTTTACTTACTTTATCTTATTATGGCGCTGAAAAAGTTATGCCAAATTATAACGTTATG
GGTTTATGTAAAGCGGCACTTGAAGCAAGTGTAAAATATATAGCATGTGATCTCGGGCCACAAAATATCAGAGTAAATGC
AATTTCCGCTGGTCCAATCAGAACTTTAGCATCTTCTGGAATAAGCGATTTTCACTCCATATCAGAATGGAATAGAAATA
ATTCTCCGCTCAGACGCAATGTTACAATAGAGGATGTTGGCAAGGCAGCGTTATACTTATTAAGCGACTTAAGTAGTGGC
ACTACTGGAGAAATTTTGCACGTTGATTCAGGATATAACGTTGTGGGAATGAAGGTAGTTGACTAA

Upstream 100 bases:

>100_bases
GGTATTAGACTTCAAGTTACTGCAAAAAATATTTTAAAACATAGTGATATCTTGACTTTACCTAGTTAATATTCCAAATT
ATAAAAAAACAGATAGTTCT

Downstream 100 bases:

>100_bases
CAAAGCACTAGTTCCTTCGGGTTCATACACAACTCTATCAGGAGTTTTGTGACTGTGATTGCAGAAAACATCGAACATAA
AATTCCTATTGATAGAGTGA

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTTSLLQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEIIKEKLSPIADELNVELILHCDVSNEKIIDNAFEII
EKKWGTLDFLVHAIAFSDKNELDGKYVNTSLDNFLNAMHISCYSFTALAQRAEKMMSNGGSLLTLSYYGAEKVMPNYNVM
GLCKAALEASVKYIACDLGPQNIRVNAISAGPIRTLASSGISDFHSISEWNRNNSPLRRNVTIEDVGKAALYLLSDLSSG
TTGEILHVDSGYNVVGMKVVD

Sequences:

>Translated_261_residues
MTTSLLQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEIIKEKLSPIADELNVELILHCDVSNEKIIDNAFEII
EKKWGTLDFLVHAIAFSDKNELDGKYVNTSLDNFLNAMHISCYSFTALAQRAEKMMSNGGSLLTLSYYGAEKVMPNYNVM
GLCKAALEASVKYIACDLGPQNIRVNAISAGPIRTLASSGISDFHSISEWNRNNSPLRRNVTIEDVGKAALYLLSDLSSG
TTGEILHVDSGYNVVGMKVVD
>Mature_260_residues
TTSLLQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEIIKEKLSPIADELNVELILHCDVSNEKIIDNAFEIIE
KKWGTLDFLVHAIAFSDKNELDGKYVNTSLDNFLNAMHISCYSFTALAQRAEKMMSNGGSLLTLSYYGAEKVMPNYNVMG
LCKAALEASVKYIACDLGPQNIRVNAISAGPIRTLASSGISDFHSISEWNRNNSPLRRNVTIEDVGKAALYLLSDLSSGT
TGEILHVDSGYNVVGMKVVD

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=238, Percent_Identity=25.2100840336134, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=50, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=29.3650793650794, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.1853281853282, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=25.5639097744361, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=26.6409266409266, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644337, Length=185, Percent_Identity=30.2702702702703, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28483; Mature: 28352

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSLLQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEIIKEKLSPIADELNVE
CCCCHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEE
LILHCDVSNEKIIDNAFEIIEKKWGTLDFLVHAIAFSDKNELDGKYVNTSLDNFLNAMHI
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHH
SCYSFTALAQRAEKMMSNGGSLLTLSYYGAEKVMPNYNVMGLCKAALEASVKYIACDLGP
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEECCC
QNIRVNAISAGPIRTLASSGISDFHSISEWNRNNSPLRRNVTIEDVGKAALYLLSDLSSG
CCEEEEEECCCCHHHHHHCCCHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCC
TTGEILHVDSGYNVVGMKVVD
CCCCEEEECCCCCEEEEEEEC
>Mature Secondary Structure 
TTSLLQGKKGLITGIINKRSIAYGIAKTLSEHGAELAITYQNEIIKEKLSPIADELNVE
CCCHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEE
LILHCDVSNEKIIDNAFEIIEKKWGTLDFLVHAIAFSDKNELDGKYVNTSLDNFLNAMHI
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHH
SCYSFTALAQRAEKMMSNGGSLLTLSYYGAEKVMPNYNVMGLCKAALEASVKYIACDLGP
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEECCC
QNIRVNAISAGPIRTLASSGISDFHSISEWNRNNSPLRRNVTIEDVGKAALYLLSDLSSG
CCEEEEEECCCCHHHHHHCCCHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCC
TTGEILHVDSGYNVVGMKVVD
CCCCEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]