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The map label for this gene is tolB [H]

Identifier: 225629902

GI number: 225629902

Start: 40653

End: 41915

Strand: Reverse

Name: tolB [H]

Synonym: WRi_000320

Alternate gene names: 225629902

Gene position: 41915-40653 (Counterclockwise)

Preceding gene: 225629903

Following gene: 225629898

Centisome position: 2.9

GC content: 33.81

Gene sequence:

>1263_bases
ATGAAGCTATTCGTTCATCTGGTGTTATTTATTTCGTTATTTATTCCTTATTTTACAAAAGCTGCTTTATATGTTGATAT
AAAAAAAAGCAGTGTTGGCAACATTGGTCTTGTTGTATCTAAATGTACATGTAAAACAGCGTTGGAAAGCGAATTAAGCG
AAAATATTGCAAAGGTAATAGGAACAAATTTATCTAATTGTGGCTTATTTAATGTAAAACGTGGCGCGGAAGCTGAATCT
AAATCTTGGAAAAGCGATACTGTAGTCACAGTAAGTTTAAGCGAAATATCTGGTAGTGCATTAGAGCTATCATTTCGTTT
ATTTGACACTTTTACAAAAAGAGAATTACTTACTCAGTCAGTTGTTTTTCCAGCAAAAGACTGGAGAAAAATTGGTCACC
TCGTTTCGGATGTGATACATGATAGATTAATTGGTGAGAAAGGGCACTTCAACACAAAAATCACGTATATCGCTGAAGAA
AAAGATAGCAACTACAAATCTGTGCGTAAAATTGCTGTGATGAATCAAGATGGAAGTAATATAAAATACTTAACAAATGG
CGATAGATTTGTGTCAACACCGAGATTCTCACCAAATGGAAAGGGTATTGTTTATATCTCGTACGCAAATGGTAAAAGTT
ATATAATATTAAAAAATTTAAAAGATAACACTGAATCAATAATCAGCACATTTGAAGGAGTTGTTTCTGCACCAAGATTT
TCTCCCGATGGTAAATCTCTTTTAATTTCCCACTCATTGGGTGGTGAAACGAATATATTATCTTTGGATTTAAGTAGTAA
ACGAACAAAAAAAATTACTAAAGGTTCAGCTATAAGCACTTCTCCCTCTTTTTCTCCAGATCAAAAGTATATGGCTTTTA
GTTCTGATATAAGTGGGAGTCAGCAACTGTACGTTATCGATTTTACTAACAAAAGTAAAAAACCTAAAAGAATTAGTTTT
GGAAGTGGAAGATATGCTACGCCTGTTTGGTCGCCAAAAGGAGATCTGATAGCTTTTACAAAGATTCAGTCAGGAAAATT
TTACATAGGAGTTATGAAGCCAGATGGCAAAGAAGAACGTCTGCTTTCAGAGGGGCATAAAATTGAATCCCCGGCATGGC
TACCAAATGGTAGAGAAATCATTTTTACAAGAACAGAATCACCAAGCAACTCTAAACTATATTTAGTAGACTTAGTAAAG
AAAAATCAAAAAATGGTTTCCACTCCCACAAATGCTTCTTTACCGGATTGGTCTTATTTTTGA

Upstream 100 bases:

>100_bases
AAATTAAAAATAAGATTGAAAGTTCAATATTGAGTATCTTAAAGGAATATTTACTAAAAAGGCCTATAATTGAGGTTCAG
ATAGAACAGGTATGAAATGA

Downstream 100 bases:

>100_bases
AAGTAGATCTATTATAGGTTTGATATGAGCCTTATTTTACTTTAATCGCTTATAACCTTTGGCACTACAAAATACCCATG
CTCAGGTTTTGTATTGGACA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKLFVHLVLFISLFIPYFTKAALYVDIKKSSVGNIGLVVSKCTCKTALESELSENIAKVIGTNLSNCGLFNVKRGAEAES
KSWKSDTVVTVSLSEISGSALELSFRLFDTFTKRELLTQSVVFPAKDWRKIGHLVSDVIHDRLIGEKGHFNTKITYIAEE
KDSNYKSVRKIAVMNQDGSNIKYLTNGDRFVSTPRFSPNGKGIVYISYANGKSYIILKNLKDNTESIISTFEGVVSAPRF
SPDGKSLLISHSLGGETNILSLDLSSKRTKKITKGSAISTSPSFSPDQKYMAFSSDISGSQQLYVIDFTNKSKKPKRISF
GSGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTRTESPSNSKLYLVDLVK
KNQKMVSTPTNASLPDWSYF

Sequences:

>Translated_420_residues
MKLFVHLVLFISLFIPYFTKAALYVDIKKSSVGNIGLVVSKCTCKTALESELSENIAKVIGTNLSNCGLFNVKRGAEAES
KSWKSDTVVTVSLSEISGSALELSFRLFDTFTKRELLTQSVVFPAKDWRKIGHLVSDVIHDRLIGEKGHFNTKITYIAEE
KDSNYKSVRKIAVMNQDGSNIKYLTNGDRFVSTPRFSPNGKGIVYISYANGKSYIILKNLKDNTESIISTFEGVVSAPRF
SPDGKSLLISHSLGGETNILSLDLSSKRTKKITKGSAISTSPSFSPDQKYMAFSSDISGSQQLYVIDFTNKSKKPKRISF
GSGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTRTESPSNSKLYLVDLVK
KNQKMVSTPTNASLPDWSYF
>Mature_420_residues
MKLFVHLVLFISLFIPYFTKAALYVDIKKSSVGNIGLVVSKCTCKTALESELSENIAKVIGTNLSNCGLFNVKRGAEAES
KSWKSDTVVTVSLSEISGSALELSFRLFDTFTKRELLTQSVVFPAKDWRKIGHLVSDVIHDRLIGEKGHFNTKITYIAEE
KDSNYKSVRKIAVMNQDGSNIKYLTNGDRFVSTPRFSPNGKGIVYISYANGKSYIILKNLKDNTESIISTFEGVVSAPRF
SPDGKSLLISHSLGGETNILSLDLSSKRTKKITKGSAISTSPSFSPDQKYMAFSSDISGSQQLYVIDFTNKSKKPKRISF
GSGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEERLLSEGHKIESPAWLPNGREIIFTRTESPSNSKLYLVDLVK
KNQKMVSTPTNASLPDWSYF

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=433, Percent_Identity=24.4803695150115, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 46580; Mature: 46580

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFVHLVLFISLFIPYFTKAALYVDIKKSSVGNIGLVVSKCTCKTALESELSENIAKVI
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GTNLSNCGLFNVKRGAEAESKSWKSDTVVTVSLSEISGSALELSFRLFDTFTKRELLTQS
CCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCEEEEEEHHHHHHHHHHHHHHH
VVFPAKDWRKIGHLVSDVIHDRLIGEKGHFNTKITYIAEEKDSNYKSVRKIAVMNQDGSN
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHEEEEEEECCCCCC
IKYLTNGDRFVSTPRFSPNGKGIVYISYANGKSYIILKNLKDNTESIISTFEGVVSAPRF
EEEEECCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
SPDGKSLLISHSLGGETNILSLDLSSKRTKKITKGSAISTSPSFSPDQKYMAFSSDISGS
CCCCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
QQLYVIDFTNKSKKPKRISFGSGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEER
CEEEEEEECCCCCCCCEEECCCCCEECCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHH
LLSEGHKIESPAWLPNGREIIFTRTESPSNSKLYLVDLVKKNQKMVSTPTNASLPDWSYF
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKLFVHLVLFISLFIPYFTKAALYVDIKKSSVGNIGLVVSKCTCKTALESELSENIAKVI
CHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GTNLSNCGLFNVKRGAEAESKSWKSDTVVTVSLSEISGSALELSFRLFDTFTKRELLTQS
CCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCEEEEEEHHHHHHHHHHHHHHH
VVFPAKDWRKIGHLVSDVIHDRLIGEKGHFNTKITYIAEEKDSNYKSVRKIAVMNQDGSN
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHEEEEEEECCCCCC
IKYLTNGDRFVSTPRFSPNGKGIVYISYANGKSYIILKNLKDNTESIISTFEGVVSAPRF
EEEEECCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
SPDGKSLLISHSLGGETNILSLDLSSKRTKKITKGSAISTSPSFSPDQKYMAFSSDISGS
CCCCCEEEEEECCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
QQLYVIDFTNKSKKPKRISFGSGRYATPVWSPKGDLIAFTKIQSGKFYIGVMKPDGKEER
CEEEEEEECCCCCCCCEEECCCCCEECCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHH
LLSEGHKIESPAWLPNGREIIFTRTESPSNSKLYLVDLVKKNQKMVSTPTNASLPDWSYF
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA