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The map label for this gene is prsA [H]

Identifier: 225629900

GI number: 225629900

Start: 39342

End: 40268

Strand: Direct

Name: prsA [H]

Synonym: WRi_000300

Alternate gene names: 225629900

Gene position: 39342-40268 (Clockwise)

Preceding gene: 225629899

Following gene: 225629901

Centisome position: 2.72

GC content: 35.17

Gene sequence:

>927_bases
ATGAAGATAATAATAGGTAATGCTAGTAAGGAATTAGGGGAATCAGTAGTTAACCGGCTAGATGTTCAACCATCCTCTGC
TTGGATATCAAAGTTTACCGATGGCGAGGTAAATGTAGAAGTAGCAAATGATCTATATAATCAAGAAGTATATATAATAC
AATCTCTTTCTCCCCCTGTGAATGATAACCTTATGGAGCTTCTGCTTATAATTGATGCAGTAAATAGATTAGGAGCCAAA
AGGATAGTAGTAATTATTCCTTATTATGGATATAGCAGACAAGATAGAATTATCAAAAACAATAATATGCAATCTGCTTT
AAGTGCTAAATTAGTTGCAAATCTTATTCAAACTGCAGGTGCAAGTAGTGTTGCAGTTATTGATTTGCACTCAAGTCAAA
TTGAAGGATTCTTTGATGTACCGATAACTAATTTGAACTGCTTTGAAGCATTTGTTGACTCTATACACACGGAAAATTTG
GCAATTGTTGCACCTGATGTTGGAGCAATTGGTAGAGCACGTGCTTTTGCAAAGACTTTAGAGGAAAAGTACAAGATAGG
GTTAAGTGATAAGATTATTGTAGTAGATAAATATAGGGAGAAAGCAGGCACATCTCAAGTAATGAACATAATAGGAGAAG
TTGCAAACAAAAATTGTGTCATTGTTGATGATATAGTTGACTCTGGTGGAACGTTGTGTAATGCAGCTCTTGCTTTAAAA
AGTTACGGAGCAAAGTCCGTAACTTCATGCATCACACACGGTGTGCTTTCAGGAAGTGCAGTTGAGAAAATCTCTTCCTC
TTCTTTAGATAAATTAGTGATTACGGATACCATACTTCACAAACTTGAAAAAACTGGTAAAATAGAAGTTGTTTCGGTTG
TAAATATTTTAACTCACTTCATCCAAGGAGGTAGAAATGCCAGCTAA

Upstream 100 bases:

>100_bases
AAGAGATACCTAATTCGCCGACAAGAATACTAGATAAAGAAACTTACATTTCAATTATTTTACAATTTCTGTATAATCTT
AAAAACTCAATTAGTGCGTT

Downstream 100 bases:

>100_bases
GTCAACAGAAGACGTTATTACTTCACTAATAGAATTTGCAAATAAGAGAAAAATAACAATTACCAAGGAAGAAATGCTTA
AAATTGCACAGCTTGTAAGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKIIIGNASKELGESVVNRLDVQPSSAWISKFTDGEVNVEVANDLYNQEVYIIQSLSPPVNDNLMELLLIIDAVNRLGAK
RIVVIIPYYGYSRQDRIIKNNNMQSALSAKLVANLIQTAGASSVAVIDLHSSQIEGFFDVPITNLNCFEAFVDSIHTENL
AIVAPDVGAIGRARAFAKTLEEKYKIGLSDKIIVVDKYREKAGTSQVMNIIGEVANKNCVIVDDIVDSGGTLCNAALALK
SYGAKSVTSCITHGVLSGSAVEKISSSSLDKLVITDTILHKLEKTGKIEVVSVVNILTHFIQGGRNAS

Sequences:

>Translated_308_residues
MKIIIGNASKELGESVVNRLDVQPSSAWISKFTDGEVNVEVANDLYNQEVYIIQSLSPPVNDNLMELLLIIDAVNRLGAK
RIVVIIPYYGYSRQDRIIKNNNMQSALSAKLVANLIQTAGASSVAVIDLHSSQIEGFFDVPITNLNCFEAFVDSIHTENL
AIVAPDVGAIGRARAFAKTLEEKYKIGLSDKIIVVDKYREKAGTSQVMNIIGEVANKNCVIVDDIVDSGGTLCNAALALK
SYGAKSVTSCITHGVLSGSAVEKISSSSLDKLVITDTILHKLEKTGKIEVVSVVNILTHFIQGGRNAS
>Mature_308_residues
MKIIIGNASKELGESVVNRLDVQPSSAWISKFTDGEVNVEVANDLYNQEVYIIQSLSPPVNDNLMELLLIIDAVNRLGAK
RIVVIIPYYGYSRQDRIIKNNNMQSALSAKLVANLIQTAGASSVAVIDLHSSQIEGFFDVPITNLNCFEAFVDSIHTENL
AIVAPDVGAIGRARAFAKTLEEKYKIGLSDKIIVVDKYREKAGTSQVMNIIGEVANKNCVIVDDIVDSGGTLCNAALALK
SYGAKSVTSCITHGVLSGSAVEKISSSSLDKLVITDTILHKLEKTGKIEVVSVVNILTHFIQGGRNAS

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI84875539, Length=309, Percent_Identity=35.5987055016181, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=34.8242811501597, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI4506129, Length=309, Percent_Identity=35.5987055016181, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=33.8658146964856, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI194018537, Length=328, Percent_Identity=29.2682926829268, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4506133, Length=329, Percent_Identity=29.483282674772, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI310128524, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310115209, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310118259, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310119946, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1787458, Length=281, Percent_Identity=41.9928825622776, Blast_Score=245, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=33.8658146964856, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=33.8658146964856, Blast_Score=190, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=33.8658146964856, Blast_Score=190, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=34.0909090909091, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17570245, Length=319, Percent_Identity=31.3479623824451, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6319403, Length=285, Percent_Identity=36.8421052631579, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=36.3057324840764, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320946, Length=284, Percent_Identity=35.9154929577465, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6322667, Length=211, Percent_Identity=32.7014218009479, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324511, Length=101, Percent_Identity=42.5742574257426, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=33.5463258785943, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45551540, Length=333, Percent_Identity=31.8318318318318, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI281362873, Length=326, Percent_Identity=27.6073619631902, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24651454, Length=326, Percent_Identity=27.6073619631902, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24651458, Length=326, Percent_Identity=27.6073619631902, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24651456, Length=326, Percent_Identity=27.6073619631902, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45552010, Length=192, Percent_Identity=30.2083333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651462, Length=192, Percent_Identity=30.2083333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651464, Length=192, Percent_Identity=30.2083333333333, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33177; Mature: 33177

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIGNASKELGESVVNRLDVQPSSAWISKFTDGEVNVEVANDLYNQEVYIIQSLSPPV
CEEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHCCCEEEEEECCCCCC
NDNLMELLLIIDAVNRLGAKRIVVIIPYYGYSRQDRIIKNNNMQSALSAKLVANLIQTAG
CHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCC
ASSVAVIDLHSSQIEGFFDVPITNLNCFEAFVDSIHTENLAIVAPDVGAIGRARAFAKTL
CCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
EEKYKIGLSDKIIVVDKYREKAGTSQVMNIIGEVANKNCVIVDDIVDSGGTLCNAALALK
HHHHCCCCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
SYGAKSVTSCITHGVLSGSAVEKISSSSLDKLVITDTILHKLEKTGKIEVVSVVNILTHF
HCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IQGGRNAS
HCCCCCCC
>Mature Secondary Structure
MKIIIGNASKELGESVVNRLDVQPSSAWISKFTDGEVNVEVANDLYNQEVYIIQSLSPPV
CEEEEECCHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHCCCEEEEEECCCCCC
NDNLMELLLIIDAVNRLGAKRIVVIIPYYGYSRQDRIIKNNNMQSALSAKLVANLIQTAG
CHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCC
ASSVAVIDLHSSQIEGFFDVPITNLNCFEAFVDSIHTENLAIVAPDVGAIGRARAFAKTL
CCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
EEKYKIGLSDKIIVVDKYREKAGTSQVMNIIGEVANKNCVIVDDIVDSGGTLCNAALALK
HHHHCCCCCCEEEEEECCHHHCCHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
SYGAKSVTSCITHGVLSGSAVEKISSSSLDKLVITDTILHKLEKTGKIEVVSVVNILTHF
HCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IQGGRNAS
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA