Click here to switch to the map view.

The map label for this gene is serS [H]

Identifier: 225629896

GI number: 225629896

Start: 32352

End: 33650

Strand: Direct

Name: serS [H]

Synonym: WRi_000260

Alternate gene names: 225629896

Gene position: 32352-33650 (Clockwise)

Preceding gene: 225629893

Following gene: 225629897

Centisome position: 2.24

GC content: 37.18

Gene sequence:

>1299_bases
ATGTTAGTAATAGCACAATATATGCACGATATAGAATATATACGCAAAAACCCTGAAGGATTTGAAAAGGCAATGAAAAG
CAGGGGAATTAGAGAATCTACTGCGGAAGAGATATTAGAAATCGATCACGAGAAAAGATCATTAACCACTAAACTGCAGG
ATTTGAATAGGCAGCGCAATGAAATTACAGAGGAAATAAAAAAGCTTAAAATGAGCAAGAGCCCCTGTGAAGAGCAAATA
GAGTTGTCAAAAAGCATCACAAATGAGATAGAGGCAATCAGCTTAAAAGAACAAGCAGAAAAGGATAAGTTAGTGAATGT
TTTATCTAACTTGCCGAATATTCCTGCGCAAGATGTGCCAATAGGCGCAGATGAGAACTCTAATTTAGAGGTGAGAAGAT
ATGGAGGAAAAAGACAGTTTGATTTTGTACCAGAATCTCATTATGAACTCGGAGAAAAATTAGGTTTAATAGACTTCGAA
CAAGCGGCGAGAATTTCCGGATCAAGATTTACGATATTAAAAGGACAGTTAGCTAAGCTTGGACGAGCGCTAATAAATTG
TATGCTTGAAATGCATGTTAATGAATTTGGCTATACTGAGGTGTACCACCCGGCTTTGGTGAAAAACGAAGCTATGTATA
ATGTTGGTCAGCTACCGAAATTTTCTGACGATTCGTATTTAACTACCGACGAATTGAGGTTGATTCCCACAAGTGAAGTG
TTTTTAACAAATTTAGTTGCTGACAAAATAATGGAAGAAAAAGAACTGCCTATTCGCTTTACTGCGTATTCAGAGTGTTT
TCGTAAAGAAGCAGGCAGCGCAGGGCGAGACACGAGAGGCATGATAAGGCAACACCAATTTGGTAAAGTGGAATTGGTGA
GTATTACAACTGAAGACCAATCAAATGATGAGCTTGAGCGTATGACGAGTGTTGCTGAAGAGATATTAAAAAAATTAGAA
TTGCCATACAGAGTAATGCTGCTTTGCAGTGGCGATATGGGTTTTGCTGCACAAAGGACTTATGACATAGAGGTATGGTT
ACCTGGGCAAAATAAATATAGAGAAATATCAAGCTGCTCAAATTGTGGTGCATTTCAAGCAAGAAGGATGAACGCTAAAT
ACTCTTCAGAAACTGATAAAAAGATAAAAAAATATGTTCACACTTTAAACGGCTCAGCTTTAGCTATAGGAAGAACGATT
ATTGCCATAATGGAGAATTATCAAAATTCTGACGGGTCGATTGCAATACCAAACGTGTTGCAAAAATACATGAGTAATGA
TACTGTTATAAGCAAATAA

Upstream 100 bases:

>100_bases
TTAATTAAATTTTAACAAATTCTAACAAAAAAATCAACCATAATAATTTTAGTAGTATGAGAATATATTGAATATTAAGA
GCTTTTGGAGTACAGTAACA

Downstream 100 bases:

>100_bases
TAATTTTGACATATAGAAAAGCGAGGAAATAAAGATGAAGGTTCTGGTTATAGGTTCTGGTGGGCGTGAGCATGCTCTAC
TTTGGGCTCTAAAAAAATCT

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLVIAQYMHDIEYIRKNPEGFEKAMKSRGIRESTAEEILEIDHEKRSLTTKLQDLNRQRNEITEEIKKLKMSKSPCEEQI
ELSKSITNEIEAISLKEQAEKDKLVNVLSNLPNIPAQDVPIGADENSNLEVRRYGGKRQFDFVPESHYELGEKLGLIDFE
QAARISGSRFTILKGQLAKLGRALINCMLEMHVNEFGYTEVYHPALVKNEAMYNVGQLPKFSDDSYLTTDELRLIPTSEV
FLTNLVADKIMEEKELPIRFTAYSECFRKEAGSAGRDTRGMIRQHQFGKVELVSITTEDQSNDELERMTSVAEEILKKLE
LPYRVMLLCSGDMGFAAQRTYDIEVWLPGQNKYREISSCSNCGAFQARRMNAKYSSETDKKIKKYVHTLNGSALAIGRTI
IAIMENYQNSDGSIAIPNVLQKYMSNDTVISK

Sequences:

>Translated_432_residues
MLVIAQYMHDIEYIRKNPEGFEKAMKSRGIRESTAEEILEIDHEKRSLTTKLQDLNRQRNEITEEIKKLKMSKSPCEEQI
ELSKSITNEIEAISLKEQAEKDKLVNVLSNLPNIPAQDVPIGADENSNLEVRRYGGKRQFDFVPESHYELGEKLGLIDFE
QAARISGSRFTILKGQLAKLGRALINCMLEMHVNEFGYTEVYHPALVKNEAMYNVGQLPKFSDDSYLTTDELRLIPTSEV
FLTNLVADKIMEEKELPIRFTAYSECFRKEAGSAGRDTRGMIRQHQFGKVELVSITTEDQSNDELERMTSVAEEILKKLE
LPYRVMLLCSGDMGFAAQRTYDIEVWLPGQNKYREISSCSNCGAFQARRMNAKYSSETDKKIKKYVHTLNGSALAIGRTI
IAIMENYQNSDGSIAIPNVLQKYMSNDTVISK
>Mature_432_residues
MLVIAQYMHDIEYIRKNPEGFEKAMKSRGIRESTAEEILEIDHEKRSLTTKLQDLNRQRNEITEEIKKLKMSKSPCEEQI
ELSKSITNEIEAISLKEQAEKDKLVNVLSNLPNIPAQDVPIGADENSNLEVRRYGGKRQFDFVPESHYELGEKLGLIDFE
QAARISGSRFTILKGQLAKLGRALINCMLEMHVNEFGYTEVYHPALVKNEAMYNVGQLPKFSDDSYLTTDELRLIPTSEV
FLTNLVADKIMEEKELPIRFTAYSECFRKEAGSAGRDTRGMIRQHQFGKVELVSITTEDQSNDELERMTSVAEEILKKLE
LPYRVMLLCSGDMGFAAQRTYDIEVWLPGQNKYREISSCSNCGAFQARRMNAKYSSETDKKIKKYVHTLNGSALAIGRTI
IAIMENYQNSDGSIAIPNVLQKYMSNDTVISK

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) [H]

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily [H]

Homologues:

Organism=Homo sapiens, GI8923421, Length=439, Percent_Identity=29.8405466970387, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI225579074, Length=441, Percent_Identity=29.9319727891156, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI16306548, Length=449, Percent_Identity=31.8485523385301, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1787120, Length=428, Percent_Identity=45.0934579439252, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17539260, Length=470, Percent_Identity=31.063829787234, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17542084, Length=329, Percent_Identity=30.6990881458967, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320226, Length=443, Percent_Identity=31.6027088036117, Blast_Score=209, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6321799, Length=412, Percent_Identity=32.2815533980583, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24581334, Length=368, Percent_Identity=33.695652173913, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17737985, Length=359, Percent_Identity=33.4261838440111, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24649556, Length=401, Percent_Identity=22.1945137157107, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.11 [H]

Molecular weight: Translated: 49229; Mature: 49229

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIAQYMHDIEYIRKNPEGFEKAMKSRGIRESTAEEILEIDHEKRSLTTKLQDLNRQRN
CEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EITEEIKKLKMSKSPCEEQIELSKSITNEIEAISLKEQAEKDKLVNVLSNLPNIPAQDVP
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IGADENSNLEVRRYGGKRQFDFVPESHYELGEKLGLIDFEQAARISGSRFTILKGQLAKL
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCEEEEEHHHHHHH
GRALINCMLEMHVNEFGYTEVYHPALVKNEAMYNVGQLPKFSDDSYLTTDELRLIPTSEV
HHHHHHHHHHHHHHHCCCCHHHCCHHHCCCHHHCCCCCCCCCCCCEEECCCEEEECCHHH
FLTNLVADKIMEEKELPIRFTAYSECFRKEAGSAGRDTRGMIRQHQFGKVELVSITTEDQ
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
SNDELERMTSVAEEILKKLELPYRVMLLCSGDMGFAAQRTYDIEVWLPGQNKYREISSCS
CCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHCC
NCGAFQARRMNAKYSSETDKKIKKYVHTLNGSALAIGRTIIAIMENYQNSDGSIAIPNVL
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHH
QKYMSNDTVISK
HHHCCCCCCCCC
>Mature Secondary Structure
MLVIAQYMHDIEYIRKNPEGFEKAMKSRGIRESTAEEILEIDHEKRSLTTKLQDLNRQRN
CEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EITEEIKKLKMSKSPCEEQIELSKSITNEIEAISLKEQAEKDKLVNVLSNLPNIPAQDVP
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IGADENSNLEVRRYGGKRQFDFVPESHYELGEKLGLIDFEQAARISGSRFTILKGQLAKL
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCEEEEEHHHHHHH
GRALINCMLEMHVNEFGYTEVYHPALVKNEAMYNVGQLPKFSDDSYLTTDELRLIPTSEV
HHHHHHHHHHHHHHHCCCCHHHCCHHHCCCHHHCCCCCCCCCCCCEEECCCEEEECCHHH
FLTNLVADKIMEEKELPIRFTAYSECFRKEAGSAGRDTRGMIRQHQFGKVELVSITTEDQ
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
SNDELERMTSVAEEILKKLELPYRVMLLCSGDMGFAAQRTYDIEVWLPGQNKYREISSCS
CCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHCC
NCGAFQARRMNAKYSSETDKKIKKYVHTLNGSALAIGRTIIAIMENYQNSDGSIAIPNVL
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHH
QKYMSNDTVISK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA