Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 225629897

GI number: 225629897

Start: 33685

End: 34956

Strand: Direct

Name: purD [H]

Synonym: WRi_000270

Alternate gene names: 225629897

Gene position: 33685-34956 (Clockwise)

Preceding gene: 225629896

Following gene: 225629899

Centisome position: 2.33

GC content: 36.95

Gene sequence:

>1272_bases
ATGAAGGTTCTGGTTATAGGTTCTGGTGGGCGTGAGCATGCTCTACTTTGGGCTCTAAAAAAATCTCCTATCTTGACTGA
GTTATATGTAACTCCTGGTCGCCAAGCTATGAAAGATCTTGGGGCTCTTGTGGATGTAAATATTCAAAATTCAGTGGATG
TTACACAATTTTGCAAGAGAGAAAATATAGAGTTGGTTGTTATTGGTCCAGAACAACCAATAATTGATGGGCTTGCTGAT
GATTTAGTTGCAGAGGGAATAAATGTTTTTGCTCCAAGTCAAGCAACTGCAAAACTTGAGGGATCGAAGTCTTTCACCAA
GGGACTATGTAAACGATATGGTATACCAACTGCCAAGTACGAGTGTTTTGTTGATGAAGGGTTAGCTAAAGATTTTGTAC
GTAGCAATAAAATAAAGTTTCCACTTGTAGTGAAAGCAAATGGAATTGCAGCAGGAAAAGGGGTGGTTATATGTAATACA
GAAAGCGAAGCTTTTTCAGCAATAAATTCAATGCTAGTGGAGAAAAAATTTGGTGAATCAGGTGAAGAAATAATCATAGA
AGAGTTTTTAGTTGGAGAAGAAGTAAGTTTTTTTGCTCTTATTGATGGGTTGAAAGTAGTAACTCTTGGGTGTGCAAAAG
ATTATAAGAGAGTTGATGAAAGTAATGAAAGCCAAAATACTGGAGGTATGGGGTCGTACTCATCACCTTCAATTATAAGT
AAGGACATGGAGCAAAAAATTATCCAAAAAATAATATACCCTACAGCTCAAGCATTGGTTAACATGGGTACGTCTTATAA
AGGAGTACTCTTTGCTGGTTTAATGATTTGCAAAGATAGCCCAAAACTTCTCGAGTATAACGTTAGATTCGGTGATCCAG
AAACGCAATCTATATTACCTAGATTTGATTCAAATTGCGATTTGTTAAAATTGATGTTGTCAGTTGCAGAAGGAAAGTTA
AATGTCAAAATGGTGGAACTTAACAACAAATCTACAGTTTGTGTAGTTGTTGCAAGTAAAGGCTATCCTGGTGATTATCA
AAAGGGAGAAGTAATTAAAGGATTAGATAAGATTGAAAGCATTCCTGGTGTGTTGGTGTTTCATGCTGGTACTAAATTGG
ATGAAAGTGGTAACTTAGTTTCCGATGGCGGAAGAGTGCTAAATATAGTAGCAGAAGGGAGCACTATAGAGGAAGCCAAA
AGTAAGGTGTACTCAGCATTAAATTTTTTAGAATGGCCAGGGGGCTTCTTCAGATACGATATCGGTAGTTAA

Upstream 100 bases:

>100_bases
GGGTCGATTGCAATACCAAACGTGTTGCAAAAATACATGAGTAATGATACTGTTATAAGCAAATAATAATTTTGACATAT
AGAAAAGCGAGGAAATAAAG

Downstream 100 bases:

>100_bases
TGTTAGCTGTCGGTTAGCAGGTGGTTCACAGAAACGAAAAACTTCCTTGACAAACTCCGCCAGCCCCCTTATCATGAAAC
TGAAGCTATTGTATTTGCTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVLVIGSGGREHALLWALKKSPILTELYVTPGRQAMKDLGALVDVNIQNSVDVTQFCKRENIELVVIGPEQPIIDGLAD
DLVAEGINVFAPSQATAKLEGSKSFTKGLCKRYGIPTAKYECFVDEGLAKDFVRSNKIKFPLVVKANGIAAGKGVVICNT
ESEAFSAINSMLVEKKFGESGEEIIIEEFLVGEEVSFFALIDGLKVVTLGCAKDYKRVDESNESQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTAQALVNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPETQSILPRFDSNCDLLKLMLSVAEGKL
NVKMVELNNKSTVCVVVASKGYPGDYQKGEVIKGLDKIESIPGVLVFHAGTKLDESGNLVSDGGRVLNIVAEGSTIEEAK
SKVYSALNFLEWPGGFFRYDIGS

Sequences:

>Translated_423_residues
MKVLVIGSGGREHALLWALKKSPILTELYVTPGRQAMKDLGALVDVNIQNSVDVTQFCKRENIELVVIGPEQPIIDGLAD
DLVAEGINVFAPSQATAKLEGSKSFTKGLCKRYGIPTAKYECFVDEGLAKDFVRSNKIKFPLVVKANGIAAGKGVVICNT
ESEAFSAINSMLVEKKFGESGEEIIIEEFLVGEEVSFFALIDGLKVVTLGCAKDYKRVDESNESQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTAQALVNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPETQSILPRFDSNCDLLKLMLSVAEGKL
NVKMVELNNKSTVCVVVASKGYPGDYQKGEVIKGLDKIESIPGVLVFHAGTKLDESGNLVSDGGRVLNIVAEGSTIEEAK
SKVYSALNFLEWPGGFFRYDIGS
>Mature_423_residues
MKVLVIGSGGREHALLWALKKSPILTELYVTPGRQAMKDLGALVDVNIQNSVDVTQFCKRENIELVVIGPEQPIIDGLAD
DLVAEGINVFAPSQATAKLEGSKSFTKGLCKRYGIPTAKYECFVDEGLAKDFVRSNKIKFPLVVKANGIAAGKGVVICNT
ESEAFSAINSMLVEKKFGESGEEIIIEEFLVGEEVSFFALIDGLKVVTLGCAKDYKRVDESNESQNTGGMGSYSSPSIIS
KDMEQKIIQKIIYPTAQALVNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPETQSILPRFDSNCDLLKLMLSVAEGKL
NVKMVELNNKSTVCVVVASKGYPGDYQKGEVIKGLDKIESIPGVLVFHAGTKLDESGNLVSDGGRVLNIVAEGSTIEEAK
SKVYSALNFLEWPGGFFRYDIGS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=41.4117647058824, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=41.4117647058824, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=41.4117647058824, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=41.4117647058824, Blast_Score=317, Evalue=1e-86,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=43.0588235294118, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=39.0588235294118, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321203, Length=436, Percent_Identity=37.1559633027523, Blast_Score=298, Evalue=8e-82,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=39.3939393939394, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=39.3939393939394, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45829; Mature: 45829

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGSGGREHALLWALKKSPILTELYVTPGRQAMKDLGALVDVNIQNSVDVTQFCKR
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCHHHHHHCCCEEEECCCCCCCHHHHHHH
ENIELVVIGPEQPIIDGLADDLVAEGINVFAPSQATAKLEGSKSFTKGLCKRYGIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHCCCCCCE
ECFVDEGLAKDFVRSNKIKFPLVVKANGIAAGKGVVICNTESEAFSAINSMLVEKKFGES
EEEHHHHHHHHHHHCCCCEEEEEEEECCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCC
GEEIIIEEFLVGEEVSFFALIDGLKVVTLGCAKDYKRVDESNESQNTGGMGSYSSPSIIS
CCCEEHHHHHCCCCCEEHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KDMEQKIIQKIIYPTAQALVNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPETQSILP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHH
RFDSNCDLLKLMLSVAEGKLNVKMVELNNKSTVCVVVASKGYPGDYQKGEVIKGLDKIES
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
IPGVLVFHAGTKLDESGNLVSDGGRVLNIVAEGSTIEEAKSKVYSALNFLEWPGGFFRYD
CCCEEEEECCCCCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEE
IGS
CCC
>Mature Secondary Structure
MKVLVIGSGGREHALLWALKKSPILTELYVTPGRQAMKDLGALVDVNIQNSVDVTQFCKR
CEEEEEECCCCCCEEEEEECCCCCEEEEEECCCHHHHHHCCCEEEECCCCCCCHHHHHHH
ENIELVVIGPEQPIIDGLADDLVAEGINVFAPSQATAKLEGSKSFTKGLCKRYGIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHCCCCCCE
ECFVDEGLAKDFVRSNKIKFPLVVKANGIAAGKGVVICNTESEAFSAINSMLVEKKFGES
EEEHHHHHHHHHHHCCCCEEEEEEEECCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCC
GEEIIIEEFLVGEEVSFFALIDGLKVVTLGCAKDYKRVDESNESQNTGGMGSYSSPSIIS
CCCEEHHHHHCCCCCEEHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
KDMEQKIIQKIIYPTAQALVNMGTSYKGVLFAGLMICKDSPKLLEYNVRFGDPETQSILP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHH
RFDSNCDLLKLMLSVAEGKLNVKMVELNNKSTVCVVVASKGYPGDYQKGEVIKGLDKIES
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHC
IPGVLVFHAGTKLDESGNLVSDGGRVLNIVAEGSTIEEAKSKVYSALNFLEWPGGFFRYD
CCCEEEEECCCCCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEE
IGS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]