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The map label for this gene is dus [H]

Identifier: 225629894

GI number: 225629894

Start: 29339

End: 30301

Strand: Reverse

Name: dus [H]

Synonym: WRi_000240

Alternate gene names: 225629894

Gene position: 30301-29339 (Counterclockwise)

Preceding gene: 225629895

Following gene: 225629882

Centisome position: 2.1

GC content: 40.6

Gene sequence:

>963_bases
ATGTCCCTACAAGTAGGAAATTTGACAATTGATTCTCCAGTTATTTTAGCTCCAATGTCTGGAGTGACTGATTATCCGTT
TAGAAGTATTGTGAAGAAACTTGGTGCAAGTTTGTTAGTTTCAGAGATGATCGCAAGCAGAGCTATGATTATGCAAACTC
GCCAGAGTTTGCAAAAAGCAAAAGTCGATGCATTAACTGCTGTGCAGCTTGCTGGGTGTGAGCCAGATGTTATGGCGGAG
GCTGCAAAATTAAACGAGGACATGGGAGCAAAAATTATAGACATAAACTTTGGTTGCCCTGTTAAAAAAGTTGTAAACGG
TTATGCAGGATCAGCGCTAATGCGTGATGAGAAAAAAGCCGCTGAGATAATTGAAGCTGTGGTCAAAGCAGTGAATATGC
CAGTTACGGTGAAAATGCGCACTGGATGGAACGATGAAAATCGCAATGCCCCGAGACTTGCGAAAATTGCCGAAGACTTA
GGAGCAAAAATGATCACTGTGCATGGAAGAACAAGAGCGCAGCTTTATAATGGTCAAGCAGATTGGAAATTCGTTAGAAA
TGTTAAAGAGCAAGTGAAAATTCCAGTTATAGTAAATGGCGATATTAAAAATTTAAATGATATTCAGAATGCTCTCAAAG
AATCTGGAGCAGACGGAGTGATGATAGGTCGTGGTGCCTACGGTAAACCTTGGCTGATTAATCAAGCAATGAACTTTCTC
AGTGGAAGTGAAACTTCTGAGCCAACAGCTTCAGAGAGATTAAGCATCATACTCGAGCATTACGACAATATTCTTGAGTA
CTATGGAAATGACACAGGAATAAAGATGGCCCGCAAACACATAGGTTGGTACAGTAGCGGGCTTCAAGGCTCATCTGAAT
TTCGTGTGAGAGTAAATAACATGACAGATAGCACAGAAGTGAAAGAAAATATTATTGGCTTTTTTGGTCAAGAGACACAC
TAG

Upstream 100 bases:

>100_bases
TAGTAGAGGTAGAGCCTTCTCTTATGGAAAAAGTAAGTTCGGCTATACAAAGTGTTGTAACAGTATGTGTAAATGCGCTA
GGTTTGAACACATAGAATAT

Downstream 100 bases:

>100_bases
CTGTACTCAATTCGACTGATCGTATGCCACTACGCTCTCTTCCTCACCAGAGTGTAAATGACAACCATTATCCATCAACC
ATTCCTCGTCATAAGTTTCA

Product: NifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSLQVGNLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSLQKAKVDALTAVQLAGCEPDVMAE
AAKLNEDMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVNMPVTVKMRTGWNDENRNAPRLAKIAEDL
GAKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKNLNDIQNALKESGADGVMIGRGAYGKPWLINQAMNFL
SGSETSEPTASERLSIILEHYDNILEYYGNDTGIKMARKHIGWYSSGLQGSSEFRVRVNNMTDSTEVKENIIGFFGQETH

Sequences:

>Translated_320_residues
MSLQVGNLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSLQKAKVDALTAVQLAGCEPDVMAE
AAKLNEDMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVNMPVTVKMRTGWNDENRNAPRLAKIAEDL
GAKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKNLNDIQNALKESGADGVMIGRGAYGKPWLINQAMNFL
SGSETSEPTASERLSIILEHYDNILEYYGNDTGIKMARKHIGWYSSGLQGSSEFRVRVNNMTDSTEVKENIIGFFGQETH
>Mature_319_residues
SLQVGNLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSLQKAKVDALTAVQLAGCEPDVMAEA
AKLNEDMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKAAEIIEAVVKAVNMPVTVKMRTGWNDENRNAPRLAKIAEDLG
AKMITVHGRTRAQLYNGQADWKFVRNVKEQVKIPVIVNGDIKNLNDIQNALKESGADGVMIGRGAYGKPWLINQAMNFLS
GSETSEPTASERLSIILEHYDNILEYYGNDTGIKMARKHIGWYSSGLQGSSEFRVRVNNMTDSTEVKENIIGFFGQETH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=286, Percent_Identity=32.5174825174825, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI239788462, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI40807366, Length=253, Percent_Identity=29.2490118577075, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31742496, Length=223, Percent_Identity=30.9417040358744, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=27.9527559055118, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=40.0630914826498, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=32.1739130434783, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI145693211, Length=301, Percent_Identity=27.2425249169435, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=284, Percent_Identity=30.2816901408451, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=31.8777292576419, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=211, Percent_Identity=28.436018957346, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=27.7777777777778, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=29.5652173913043, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=25.8771929824561, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=287, Percent_Identity=30.3135888501742, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24580595, Length=228, Percent_Identity=32.0175438596491, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19920448, Length=228, Percent_Identity=32.0175438596491, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921524, Length=312, Percent_Identity=26.2820512820513, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45549423, Length=234, Percent_Identity=29.0598290598291, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35110; Mature: 34979

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQVGNLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSLQKA
CCEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDALTAVQLAGCEPDVMAEAAKLNEDMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKA
HHHHHHHHHHCCCCHHHHHHHHHCCHHHCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHH
AEIIEAVVKAVNMPVTVKMRTGWNDENRNAPRLAKIAEDLGAKMITVHGRTRAQLYNGQA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCC
DWKFVRNVKEQVKIPVIVNGDIKNLNDIQNALKESGADGVMIGRGAYGKPWLINQAMNFL
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
SGSETSEPTASERLSIILEHYDNILEYYGNDTGIKMARKHIGWYSSGLQGSSEFRVRVNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCEEEEEECC
MTDSTEVKENIIGFFGQETH
CCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLQVGNLTIDSPVILAPMSGVTDYPFRSIVKKLGASLLVSEMIASRAMIMQTRQSLQKA
CEEECCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDALTAVQLAGCEPDVMAEAAKLNEDMGAKIIDINFGCPVKKVVNGYAGSALMRDEKKA
HHHHHHHHHHCCCCHHHHHHHHHCCHHHCCEEEEEECCCCHHHHHCCCCCHHHHHHHHHH
AEIIEAVVKAVNMPVTVKMRTGWNDENRNAPRLAKIAEDLGAKMITVHGRTRAQLYNGQA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCC
DWKFVRNVKEQVKIPVIVNGDIKNLNDIQNALKESGADGVMIGRGAYGKPWLINQAMNFL
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
SGSETSEPTASERLSIILEHYDNILEYYGNDTGIKMARKHIGWYSSGLQGSSEFRVRVNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCEEEEEECC
MTDSTEVKENIIGFFGQETH
CCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA