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The map label for this gene is hemK [H]

Identifier: 225629882

GI number: 225629882

Start: 11460

End: 12314

Strand: Reverse

Name: hemK [H]

Synonym: WRi_000110

Alternate gene names: 225629882

Gene position: 12314-11460 (Counterclockwise)

Preceding gene: 225629894

Following gene: 225629874

Centisome position: 0.85

GC content: 34.5

Gene sequence:

>855_bases
ATGAAAACAATCAGCACTTTAATTCAAGAAGGATCGGAGTTATTATCGTCACATAAAGTTGAATCACCACATTTAGATTG
TGAAATCATCATGCAGCACGTACTTGGAGTGGAAAGATCATTTATAATTGTGAACCATGCTGATCAAGTGCCGATGGAGA
AAGAACTTTTATTTTGGAAATTAACAAAAAAAAGAGCGGAAAGATATCCAATATCGCAAATAATAGGTAATCGTGAATTC
TGGAGTAAAAATTTTATAGTTAATCAGCATGTTTTGGATCCAAGACCAGATAGTGAAACATTAATCTCAACAGTGTTAAA
ATATTACCCAAATAAAAAGCAGAGACTAAAAATTGCAGATTTTGGCACAGGCACAGGTTGTTTATTGATATCCGTACTTA
GTGAGTATAAATATGCTGTTGGCGTCGGTTTTGAAAAAAGCTTGGAAGCATACAAAGTTGCCTATCAGAACACAAAAAAA
CATGATCTTCTCAGCAGAGCCAAAATATTTCCAAGTTCGTGGATAGAGTGCAGCGATTTATTTGATCTGATAATTAGCAA
TCCTCCATATATTAAAAGAAGTAAATTGAAAGACTTGCAAGCCGAAGTACAAAAGGAACCAAAAACTGCCCTTGATGGTG
GTATTGATGGTTTGAACTGTTACTTGAGCATTTTTCCAATATTAAGGAGGTGTCTTAAAAAAAATGGGTTTGCGATATTG
GAGATAGGTGAAGATCAAAGCAATATTGATAAAATAATCCCCTCATACGAATTAGCTTTTCAGGAATATGTATATGATTT
GGCAGGAATGAAGAGATGCATTGTTGTGAAGCAATGTATAGCTCCGCTTCTGTAA

Upstream 100 bases:

>100_bases
TTCGTACAGCTGCGTGCTTGACACTGGGATTTAGTGCAGCTACCCTAAACTGGAAGCTCATCTTGCTATTTTTGGCTGAA
AGAAACATATTTTTAGAATA

Downstream 100 bases:

>100_bases
TCCTATGATAGGATCCAGTCTTATAGCTAAGACGATTTGAAAAATCGTCATTCTGCTACGTGTTAGCGGAATCTATACCG
CGAATGAATCGCGGTATGAC

Product: modification methylase, HemK family

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKTISTLIQEGSELLSSHKVESPHLDCEIIMQHVLGVERSFIIVNHADQVPMEKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETLISTVLKYYPNKKQRLKIADFGTGTGCLLISVLSEYKYAVGVGFEKSLEAYKVAYQNTKK
HDLLSRAKIFPSSWIECSDLFDLIISNPPYIKRSKLKDLQAEVQKEPKTALDGGIDGLNCYLSIFPILRRCLKKNGFAIL
EIGEDQSNIDKIIPSYELAFQEYVYDLAGMKRCIVVKQCIAPLL

Sequences:

>Translated_284_residues
MKTISTLIQEGSELLSSHKVESPHLDCEIIMQHVLGVERSFIIVNHADQVPMEKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETLISTVLKYYPNKKQRLKIADFGTGTGCLLISVLSEYKYAVGVGFEKSLEAYKVAYQNTKK
HDLLSRAKIFPSSWIECSDLFDLIISNPPYIKRSKLKDLQAEVQKEPKTALDGGIDGLNCYLSIFPILRRCLKKNGFAIL
EIGEDQSNIDKIIPSYELAFQEYVYDLAGMKRCIVVKQCIAPLL
>Mature_284_residues
MKTISTLIQEGSELLSSHKVESPHLDCEIIMQHVLGVERSFIIVNHADQVPMEKELLFWKLTKKRAERYPISQIIGNREF
WSKNFIVNQHVLDPRPDSETLISTVLKYYPNKKQRLKIADFGTGTGCLLISVLSEYKYAVGVGFEKSLEAYKVAYQNTKK
HDLLSRAKIFPSSWIECSDLFDLIISNPPYIKRSKLKDLQAEVQKEPKTALDGGIDGLNCYLSIFPILRRCLKKNGFAIL
EIGEDQSNIDKIIPSYELAFQEYVYDLAGMKRCIVVKQCIAPLL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787463, Length=229, Percent_Identity=33.1877729257642, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082085, Length=219, Percent_Identity=27.8538812785388, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582226, Length=257, Percent_Identity=30.3501945525292, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32461; Mature: 32461

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00092 N6_MTASE ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTISTLIQEGSELLSSHKVESPHLDCEIIMQHVLGVERSFIIVNHADQVPMEKELLFWK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
LTKKRAERYPISQIIGNREFWSKNFIVNQHVLDPRPDSETLISTVLKYYPNKKQRLKIAD
HHHHHHHHCCHHHHHCCHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
FGTGTGCLLISVLSEYKYAVGVGFEKSLEAYKVAYQNTKKHDLLSRAKIFPSSWIECSDL
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHH
FDLIISNPPYIKRSKLKDLQAEVQKEPKTALDGGIDGLNCYLSIFPILRRCLKKNGFAIL
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
EIGEDQSNIDKIIPSYELAFQEYVYDLAGMKRCIVVKQCIAPLL
EECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTISTLIQEGSELLSSHKVESPHLDCEIIMQHVLGVERSFIIVNHADQVPMEKELLFWK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
LTKKRAERYPISQIIGNREFWSKNFIVNQHVLDPRPDSETLISTVLKYYPNKKQRLKIAD
HHHHHHHHCCHHHHHCCHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
FGTGTGCLLISVLSEYKYAVGVGFEKSLEAYKVAYQNTKKHDLLSRAKIFPSSWIECSDL
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHH
FDLIISNPPYIKRSKLKDLQAEVQKEPKTALDGGIDGLNCYLSIFPILRRCLKKNGFAIL
HHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
EIGEDQSNIDKIIPSYELAFQEYVYDLAGMKRCIVVKQCIAPLL
EECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]