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The map label for this gene is dhaAF [H]

Identifier: 222527031

GI number: 222527031

Start: 4743585

End: 4744466

Strand: Reverse

Name: dhaAF [H]

Synonym: Chy400_3810

Alternate gene names: 222527031

Gene position: 4744466-4743585 (Counterclockwise)

Preceding gene: 222527033

Following gene: 222527030

Centisome position: 90.05

GC content: 56.8

Gene sequence:

>882_bases
ATGGTGATACATACCGAAGTACCGGCACCGATCCGCGCACTCTTCCCGTTTCACGTACACGAATATGCTCTGCCTGCCGG
TGTGATGCGCTACGTGGATGAAGGCAGTGGGACACCGGTGGTTTTGCTGCATGGCAATCCGACATGGTCGTTCTTCTATC
GTCGCCTGATCCCGCTGCTTCGCACACAACGGCGGGTGATTGCGCCTGATCACCTGGGATGTGGATTGTCGGATAAGCCG
CAACATTATCGCTACTCGCTCGTCAATCATATCGCCAACCTGGAGAGCCTGCTGACCTGGCTTGATGTTGGGCCGGTTGA
TCTGGTTGTGCATGATTGGGGTGGTGCGATTGGAATGGGGTGGGCGGTACGCCATCCCGATTTGGTGCGGCGGATCGTTG
TGCTGAATACGGCAGCATTCCTCTCGCCCCATGTGCCGTTGCGAATTGCCGCCGGCAAGTTGCCCAGGCTGGGAGAATGG
GCCATCCGGCAACTTAATGCGTTTGCCATCGCAGCAACCACCATGGCAGTTACCCGCCCGTTACCAGCAGCGGTGCGCGA
GGGCTATCTCTGGCCTTACCGGACGTTCGCCGACCGGATTGCGATTGCTCGTTTTGTACAAGACATTCCACTGCATCCCG
GCCATCCAACATGGCCTGTAGTTGATGCGATTGATCGTGAGCTGGTACATCTGCGTGATAAACCGGTGCGAATTTTTTGG
GGTGGACGGGACTGGTGTTTTGATGATCGGTTTCTGGCCGGCTGGCTACACCGGTTTCCCTCTGCCCACGTTACCCGTCT
CGATGATGCCGGGCATTATGTGTTAGAAGACGCTTCCGCTGAGATGATTGCGCTGCTGGCGCACTGGTTGTTGTCAGAGT
AA

Upstream 100 bases:

>100_bases
AACTCGCCAATGAGTCGTCCCCGAAGACACAACGCACAATGCGTAATCGGTGTATACTCACATATAGGCAAACGCTGTGT
TATTGGAGCACGTGTCTCGC

Downstream 100 bases:

>100_bases
GAGGGGAATGTGTATGCGCGTAGAGTTACCTCAACCTCCCGTGGCTGATCGTAAGCCGGTGGTGATCCGGATACAGGGTG
ATGAGATTGTTGATGAGTTT

Product: Haloalkane dehalogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MVIHTEVPAPIRALFPFHVHEYALPAGVMRYVDEGSGTPVVLLHGNPTWSFFYRRLIPLLRTQRRVIAPDHLGCGLSDKP
QHYRYSLVNHIANLESLLTWLDVGPVDLVVHDWGGAIGMGWAVRHPDLVRRIVVLNTAAFLSPHVPLRIAAGKLPRLGEW
AIRQLNAFAIAATTMAVTRPLPAAVREGYLWPYRTFADRIAIARFVQDIPLHPGHPTWPVVDAIDRELVHLRDKPVRIFW
GGRDWCFDDRFLAGWLHRFPSAHVTRLDDAGHYVLEDASAEMIALLAHWLLSE

Sequences:

>Translated_293_residues
MVIHTEVPAPIRALFPFHVHEYALPAGVMRYVDEGSGTPVVLLHGNPTWSFFYRRLIPLLRTQRRVIAPDHLGCGLSDKP
QHYRYSLVNHIANLESLLTWLDVGPVDLVVHDWGGAIGMGWAVRHPDLVRRIVVLNTAAFLSPHVPLRIAAGKLPRLGEW
AIRQLNAFAIAATTMAVTRPLPAAVREGYLWPYRTFADRIAIARFVQDIPLHPGHPTWPVVDAIDRELVHLRDKPVRIFW
GGRDWCFDDRFLAGWLHRFPSAHVTRLDDAGHYVLEDASAEMIALLAHWLLSE
>Mature_293_residues
MVIHTEVPAPIRALFPFHVHEYALPAGVMRYVDEGSGTPVVLLHGNPTWSFFYRRLIPLLRTQRRVIAPDHLGCGLSDKP
QHYRYSLVNHIANLESLLTWLDVGPVDLVVHDWGGAIGMGWAVRHPDLVRRIVVLNTAAFLSPHVPLRIAAGKLPRLGEW
AIRQLNAFAIAATTMAVTRPLPAAVREGYLWPYRTFADRIAIARFVQDIPLHPGHPTWPVVDAIDRELVHLRDKPVRIFW
GGRDWCFDDRFLAGWLHRFPSAHVTRLDDAGHYVLEDASAEMIALLAHWLLSE

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324392, Length=107, Percent_Identity=34.5794392523364, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 33189; Mature: 33189

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIHTEVPAPIRALFPFHVHEYALPAGVMRYVDEGSGTPVVLLHGNPTWSFFYRRLIPLL
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
RTQRRVIAPDHLGCGLSDKPQHYRYSLVNHIANLESLLTWLDVGPVDLVVHDWGGAIGMG
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
WAVRHPDLVRRIVVLNTAAFLSPHVPLRIAAGKLPRLGEWAIRQLNAFAIAATTMAVTRP
EEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LPAAVREGYLWPYRTFADRIAIARFVQDIPLHPGHPTWPVVDAIDRELVHLRDKPVRIFW
CCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GGRDWCFDDRFLAGWLHRFPSAHVTRLDDAGHYVLEDASAEMIALLAHWLLSE
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIHTEVPAPIRALFPFHVHEYALPAGVMRYVDEGSGTPVVLLHGNPTWSFFYRRLIPLL
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
RTQRRVIAPDHLGCGLSDKPQHYRYSLVNHIANLESLLTWLDVGPVDLVVHDWGGAIGMG
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
WAVRHPDLVRRIVVLNTAAFLSPHVPLRIAAGKLPRLGEWAIRQLNAFAIAATTMAVTRP
EEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LPAAVREGYLWPYRTFADRIAIARFVQDIPLHPGHPTWPVVDAIDRELVHLRDKPVRIFW
CCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GGRDWCFDDRFLAGWLHRFPSAHVTRLDDAGHYVLEDASAEMIALLAHWLLSE
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]