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The map label for this gene is mls [H]

Identifier: 222526447

GI number: 222526447

Start: 4010379

End: 4011977

Strand: Reverse

Name: mls [H]

Synonym: Chy400_3214

Alternate gene names: 222526447

Gene position: 4011977-4010379 (Counterclockwise)

Preceding gene: 222526450

Following gene: 222526446

Centisome position: 76.14

GC content: 56.79

Gene sequence:

>1599_bases
ATGACCACCTCCCGGTTACCGGCTGGCGTGACGATTACCGCACCGATTACTCCCGCATACGCCGAGATTCTCACTCCCGA
AGCACTGGAATTTCTGGCCACACTCCACCGCCGCTTCAATACCCGTCGTCTGGAACTGCTTGCCCGCCGCGCTGAACGTC
AACGCGCAATTGATGCAGGCGAGCGACCGGATTTTCTGCCTGAGACAGCCCATATCCGCGAGAGCGAGTGGACGATTGCC
CCCATCCCACCCCAGATTCAGGATCGGCGCGTTGAGATTACCGGCCCGGTTGACCGTAAGATGATCATCAATGCGCTCAA
TTCAGGGGCAAAGGTGTTCATGGCCGATTTTGAAGATGCCAATACGCCAACCTGGCAGAATCAGGTTGAAGGCCAGATCA
ACCTGCGCGATGCTCTGCGTCGTACCATTACATATACCAGCCCTGAAGGCAAGCACTACGCCCTGAACAACAATCCGGCC
ATCCTGTTTGTTCGCCCGCGGGGCTGGCATCTCAACGAAAAGCATATGCTGGTTGATGGTGAGCCGATCTCTGGTGCTAT
CTTCGACTTCGGTCTCTACTTCTTCCACAATGCCCAGACTGCCATCGATGTGCAGGGTGGCCCCTATTTCTACCTGCCCA
AAATGGAGAGCCACCTCGAAGCACGGTTGTGGAATGACATCTTTGTGCTGGCCCAGGAGTTGCGCGGCATTCCCCAGGGA
ACTATCAAGGCCACGGTCTTGATCGAAACGATTCTGGCGGCGTTCGAGATGGACGAGATTCTCTACGAACTGCGCAACCA
CTCAGCCGGTCTGAATTGTGGACGCTGGGATTATATCTTCTCGTGTATCAAAAAGTTCCGCAACGACCCCAACTTCTGTC
TGGCCGACCGGGCACTGGTTACGATGACTACCCACTTCATGCGTTCATATTCACTGCTGGCGATCAAGACCTGTCACCGC
CGTGGCGCCCACGCGATGGGTGGTATGGCGGCTCAGATTCCGATTAAGAACGATCCGGTTGCCAATGAAGCAGCGCTGGC
AAAAGTGCGGGCCGACAAGGAGCGTGAAGCCTCTGACGGCCACGATGGCACCTGGGTGGCCCACCCCGGTCTGGTTCCGG
TGGCGATGGAAGTGTTCGACCGGCTAATGCCAACGCCGAACCAGATTAACCGTCAGCGTGATGATGTGCAGGTAACTGCT
GCCGATCTCCTGGCATTTGGGCCGGAAAAACCGATCACCGAGCAGGGTATGCGCCTGAATATCAACGTTGGGATCCAGTA
CCTCGGCGCCTGGCTGGCCGGCAACGGCTGTGTTCCGGTCTTCAACCTGATGGAAGATGCCGCCACCGCCGAGATTTCGC
GTGCCCAGATCTGGCAATGGATTCGCAGCCCGAAGGGTGTACTGGAGGATGGTCGCAAAGTGACGGTGGAACTCTTCCGG
CAGATGCTGCCTGAAGAGCTGCACAAGGTTCGTGAGATACTCGGTCCCGCCTACGAAGACGGTCGCTACGAAGAGGCTGC
CGAGCTGTTTGACGAGATTACAACCGATCCAGAGTTTGTGGAATTCCTGACCTTACCGGCTTACGAACGGATTCCGTAA

Upstream 100 bases:

>100_bases
TATTTCGGCACACACAACCTTGCCGCTGCACAACGTGATAACCGATTTCTGGTATAATGACTGGCGAAAAGGTTTTTCAC
ATTATGAAAGGAGGGTTTGC

Downstream 100 bases:

>100_bases
CTAGTGTCTGGTAACCTGTCAGAAACGCCATGCAGGGGTAGAGATTGCTCTACCCCTGCATGCTTACCGGATTTAGCCGC
TGTTCAGAACCTATGCGAGG

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTTSRLPAGVTITAPITPAYAEILTPEALEFLATLHRRFNTRRLELLARRAERQRAIDAGERPDFLPETAHIRESEWTIA
PIPPQIQDRRVEITGPVDRKMIINALNSGAKVFMADFEDANTPTWQNQVEGQINLRDALRRTITYTSPEGKHYALNNNPA
ILFVRPRGWHLNEKHMLVDGEPISGAIFDFGLYFFHNAQTAIDVQGGPYFYLPKMESHLEARLWNDIFVLAQELRGIPQG
TIKATVLIETILAAFEMDEILYELRNHSAGLNCGRWDYIFSCIKKFRNDPNFCLADRALVTMTTHFMRSYSLLAIKTCHR
RGAHAMGGMAAQIPIKNDPVANEAALAKVRADKEREASDGHDGTWVAHPGLVPVAMEVFDRLMPTPNQINRQRDDVQVTA
ADLLAFGPEKPITEQGMRLNINVGIQYLGAWLAGNGCVPVFNLMEDAATAEISRAQIWQWIRSPKGVLEDGRKVTVELFR
QMLPEELHKVREILGPAYEDGRYEEAAELFDEITTDPEFVEFLTLPAYERIP

Sequences:

>Translated_532_residues
MTTSRLPAGVTITAPITPAYAEILTPEALEFLATLHRRFNTRRLELLARRAERQRAIDAGERPDFLPETAHIRESEWTIA
PIPPQIQDRRVEITGPVDRKMIINALNSGAKVFMADFEDANTPTWQNQVEGQINLRDALRRTITYTSPEGKHYALNNNPA
ILFVRPRGWHLNEKHMLVDGEPISGAIFDFGLYFFHNAQTAIDVQGGPYFYLPKMESHLEARLWNDIFVLAQELRGIPQG
TIKATVLIETILAAFEMDEILYELRNHSAGLNCGRWDYIFSCIKKFRNDPNFCLADRALVTMTTHFMRSYSLLAIKTCHR
RGAHAMGGMAAQIPIKNDPVANEAALAKVRADKEREASDGHDGTWVAHPGLVPVAMEVFDRLMPTPNQINRQRDDVQVTA
ADLLAFGPEKPITEQGMRLNINVGIQYLGAWLAGNGCVPVFNLMEDAATAEISRAQIWQWIRSPKGVLEDGRKVTVELFR
QMLPEELHKVREILGPAYEDGRYEEAAELFDEITTDPEFVEFLTLPAYERIP
>Mature_531_residues
TTSRLPAGVTITAPITPAYAEILTPEALEFLATLHRRFNTRRLELLARRAERQRAIDAGERPDFLPETAHIRESEWTIAP
IPPQIQDRRVEITGPVDRKMIINALNSGAKVFMADFEDANTPTWQNQVEGQINLRDALRRTITYTSPEGKHYALNNNPAI
LFVRPRGWHLNEKHMLVDGEPISGAIFDFGLYFFHNAQTAIDVQGGPYFYLPKMESHLEARLWNDIFVLAQELRGIPQGT
IKATVLIETILAAFEMDEILYELRNHSAGLNCGRWDYIFSCIKKFRNDPNFCLADRALVTMTTHFMRSYSLLAIKTCHRR
GAHAMGGMAAQIPIKNDPVANEAALAKVRADKEREASDGHDGTWVAHPGLVPVAMEVFDRLMPTPNQINRQRDDVQVTAA
DLLAFGPEKPITEQGMRLNINVGIQYLGAWLAGNGCVPVFNLMEDAATAEISRAQIWQWIRSPKGVLEDGRKVTVELFRQ
MLPEELHKVREILGPAYEDGRYEEAAELFDEITTDPEFVEFLTLPAYERIP

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=522, Percent_Identity=54.0229885057471, Blast_Score=552, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17561814, Length=525, Percent_Identity=54.8571428571429, Blast_Score=574, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71982926, Length=419, Percent_Identity=57.2792362768496, Blast_Score=483, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6324212, Length=523, Percent_Identity=53.5372848948375, Blast_Score=554, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6322222, Length=523, Percent_Identity=52.7724665391969, Blast_Score=547, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60138; Mature: 60007

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSRLPAGVTITAPITPAYAEILTPEALEFLATLHRRFNTRRLELLARRAERQRAIDAG
CCCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
ERPDFLPETAHIRESEWTIAPIPPQIQDRRVEITGPVDRKMIINALNSGAKVFMADFEDA
CCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCC
NTPTWQNQVEGQINLRDALRRTITYTSPEGKHYALNNNPAILFVRPRGWHLNEKHMLVDG
CCCCCCCCCCCEEEHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCCCCCCCEEEECC
EPISGAIFDFGLYFFHNAQTAIDVQGGPYFYLPKMESHLEARLWNDIFVLAQELRGIPQG
CCCCCHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIKATVLIETILAAFEMDEILYELRNHSAGLNCGRWDYIFSCIKKFRNDPNFCLADRALV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHH
TMTTHFMRSYSLLAIKTCHRRGAHAMGGMAAQIPIKNDPVANEAALAKVRADKEREASDG
HHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCCHHHHHHHHCCCHHCCCCC
HDGTWVAHPGLVPVAMEVFDRLMPTPNQINRQRDDVQVTAADLLAFGPEKPITEQGMRLN
CCCEEEECCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCEEE
INVGIQYLGAWLAGNGCVPVFNLMEDAATAEISRAQIWQWIRSPKGVLEDGRKVTVELFR
EEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
QMLPEELHKVREILGPAYEDGRYEEAAELFDEITTDPEFVEFLTLPAYERIP
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TTSRLPAGVTITAPITPAYAEILTPEALEFLATLHRRFNTRRLELLARRAERQRAIDAG
CCCCCCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
ERPDFLPETAHIRESEWTIAPIPPQIQDRRVEITGPVDRKMIINALNSGAKVFMADFEDA
CCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCC
NTPTWQNQVEGQINLRDALRRTITYTSPEGKHYALNNNPAILFVRPRGWHLNEKHMLVDG
CCCCCCCCCCCEEEHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCCCCCCCEEEECC
EPISGAIFDFGLYFFHNAQTAIDVQGGPYFYLPKMESHLEARLWNDIFVLAQELRGIPQG
CCCCCHHHHHHHHHHCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIKATVLIETILAAFEMDEILYELRNHSAGLNCGRWDYIFSCIKKFRNDPNFCLADRALV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHH
TMTTHFMRSYSLLAIKTCHRRGAHAMGGMAAQIPIKNDPVANEAALAKVRADKEREASDG
HHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCCHHHHHHHHCCCHHCCCCC
HDGTWVAHPGLVPVAMEVFDRLMPTPNQINRQRDDVQVTAADLLAFGPEKPITEQGMRLN
CCCEEEECCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEHHHHHHCCCCCCCCCCCCEEE
INVGIQYLGAWLAGNGCVPVFNLMEDAATAEISRAQIWQWIRSPKGVLEDGRKVTVELFR
EEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
QMLPEELHKVREILGPAYEDGRYEEAAELFDEITTDPEFVEFLTLPAYERIP
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA