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The map label for this gene is rffG [H]

Identifier: 222526446

GI number: 222526446

Start: 4008727

End: 4009650

Strand: Reverse

Name: rffG [H]

Synonym: Chy400_3213

Alternate gene names: 222526446

Gene position: 4009650-4008727 (Counterclockwise)

Preceding gene: 222526447

Following gene: 222526443

Centisome position: 76.1

GC content: 57.14

Gene sequence:

>924_bases
ATGACTCATTACTTGATTGCCGGCGCGGCGGGATACATCGGTTCGCGACTGGCAGCGCGTCTCTTAGCCGCCGGCCATCG
CGTGCGCGGTCTTGTCTACAGCGCCGAAGATCCAGTTGTTGAACGCCTTGCCGCTCGTGGGATGGCAGTCTGGATCGGTG
ATCTCACCCGCCCCGAAACCATCGATGGGATTACTGACGAGATTGACGTTGTTTTCAATCTCACGAACCGGATGCCAATC
GATAATCCGGCACTGCTACGCGCCCTCTACATTGATGGCAACCAGCATCTGATTGCGATGTCGGTCCGCTCTCGCACGGT
GCGTACCTACCTGTGGGCGTCGAGTATCGCCGCCTACGGCGATCACGGCGAGAGCTGGGTTGACGAAGATAGCCCGGTTG
CACCCTCTTATCCGCTTGGCTCCATCTTTGCCACCGCCGAACAGACGGTATTGCAGGCAGTACGAGCCTATCGCTTCCCG
GCCATTATCTTGCGTATGGCTACCGTCTATGGCCCCGACCGTGATCCACTTGAAGCGATTGCCAGTGGTCAGTTAACGAT
TTACGGTAATGGACGCAACTTCGTCCCCCATATTCACGTTGACGATGCAGTAGAGGTATTATTGCGGGCTGCCGAAGTTG
GTCAAATCGGTGCGATTTACAATGTCGGTGATGACGAGCCACTACGCCTGATCGATCTTGTCAGTGAAGTGCGACGCCGA
CTTGGGATGTTGCCACCACGCACGTTCGATCCCAATGTCGGGCTGCGCGCCGGTATCGACCGCTCGGTGATTGGGGCATT
AACCGCATCGGTTCGCCTGAGTAATGCCCGTATGCGTCACGATTTGGGGATCGAGTTGCACTATCCCGGTCTACGCTACT
GGATCGAGGAGCGATTGCCGCTTGAGCTGGCAGTTGGGGTGTGA

Upstream 100 bases:

>100_bases
GCACCTTCACAGACAAGCACCGCATCCACCGATGTGCAGCCTGCTATGACAGTCGGGCGGTGTCAGTGAAGTCACCCGCC
CCTACACGACGAGGTGACCT

Downstream 100 bases:

>100_bases
CAGGTGTATCCTTCTGAGACGGGCGGCTGAGTCGCTGTCGGGGAGGATGAATGAACAGCGCGTTTCGTGCTCTCGCGTTA
TGAGGCTGACCGGCGGGCAC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTHYLIAGAAGYIGSRLAARLLAAGHRVRGLVYSAEDPVVERLAARGMAVWIGDLTRPETIDGITDEIDVVFNLTNRMPI
DNPALLRALYIDGNQHLIAMSVRSRTVRTYLWASSIAAYGDHGESWVDEDSPVAPSYPLGSIFATAEQTVLQAVRAYRFP
AIILRMATVYGPDRDPLEAIASGQLTIYGNGRNFVPHIHVDDAVEVLLRAAEVGQIGAIYNVGDDEPLRLIDLVSEVRRR
LGMLPPRTFDPNVGLRAGIDRSVIGALTASVRLSNARMRHDLGIELHYPGLRYWIEERLPLELAVGV

Sequences:

>Translated_307_residues
MTHYLIAGAAGYIGSRLAARLLAAGHRVRGLVYSAEDPVVERLAARGMAVWIGDLTRPETIDGITDEIDVVFNLTNRMPI
DNPALLRALYIDGNQHLIAMSVRSRTVRTYLWASSIAAYGDHGESWVDEDSPVAPSYPLGSIFATAEQTVLQAVRAYRFP
AIILRMATVYGPDRDPLEAIASGQLTIYGNGRNFVPHIHVDDAVEVLLRAAEVGQIGAIYNVGDDEPLRLIDLVSEVRRR
LGMLPPRTFDPNVGLRAGIDRSVIGALTASVRLSNARMRHDLGIELHYPGLRYWIEERLPLELAVGV
>Mature_306_residues
THYLIAGAAGYIGSRLAARLLAAGHRVRGLVYSAEDPVVERLAARGMAVWIGDLTRPETIDGITDEIDVVFNLTNRMPID
NPALLRALYIDGNQHLIAMSVRSRTVRTYLWASSIAAYGDHGESWVDEDSPVAPSYPLGSIFATAEQTVLQAVRAYRFPA
IILRMATVYGPDRDPLEAIASGQLTIYGNGRNFVPHIHVDDAVEVLLRAAEVGQIGAIYNVGDDEPLRLIDLVSEVRRRL
GMLPPRTFDPNVGLRAGIDRSVIGALTASVRLSNARMRHDLGIELHYPGLRYWIEERLPLELAVGV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=256, Percent_Identity=26.171875, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI48994969, Length=286, Percent_Identity=25.1748251748252, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 33726; Mature: 33595

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYLIAGAAGYIGSRLAARLLAAGHRVRGLVYSAEDPVVERLAARGMAVWIGDLTRPET
CCEEEEECHHHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHCCEEEEEECCCCCCC
IDGITDEIDVVFNLTNRMPIDNPALLRALYIDGNQHLIAMSVRSRTVRTYLWASSIAAYG
CCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHC
DHGESWVDEDSPVAPSYPLGSIFATAEQTVLQAVRAYRFPAIILRMATVYGPDRDPLEAI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ASGQLTIYGNGRNFVPHIHVDDAVEVLLRAAEVGQIGAIYNVGDDEPLRLIDLVSEVRRR
HCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LGMLPPRTFDPNVGLRAGIDRSVIGALTASVRLSNARMRHDLGIELHYPGLRYWIEERLP
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHCCCEEEECCHHHHHHHCCCC
LELAVGV
EEEECCC
>Mature Secondary Structure 
THYLIAGAAGYIGSRLAARLLAAGHRVRGLVYSAEDPVVERLAARGMAVWIGDLTRPET
CEEEEECHHHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHHHHCCEEEEEECCCCCCC
IDGITDEIDVVFNLTNRMPIDNPALLRALYIDGNQHLIAMSVRSRTVRTYLWASSIAAYG
CCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHC
DHGESWVDEDSPVAPSYPLGSIFATAEQTVLQAVRAYRFPAIILRMATVYGPDRDPLEAI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ASGQLTIYGNGRNFVPHIHVDDAVEVLLRAAEVGQIGAIYNVGDDEPLRLIDLVSEVRRR
HCCCEEEEECCCCCCCEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LGMLPPRTFDPNVGLRAGIDRSVIGALTASVRLSNARMRHDLGIELHYPGLRYWIEERLP
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCHHHHCCCEEEECCHHHHHHHCCCC
LELAVGV
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]