Click here to switch to the map view.

The map label for this gene is ppdK [H]

Identifier: 222526441

GI number: 222526441

Start: 4001293

End: 4003923

Strand: Reverse

Name: ppdK [H]

Synonym: Chy400_3208

Alternate gene names: 222526441

Gene position: 4003923-4001293 (Counterclockwise)

Preceding gene: 222526443

Following gene: 222526440

Centisome position: 75.99

GC content: 58.8

Gene sequence:

>2631_bases
ATGACAAAGAAGTGGGTCTACCTCTTCCGGGAAGGAAATGCGTCGATGCGCGATCTCCTTGGCGGGAAGGGGGCAGGTGT
CGCCGAGATGACACGAACCGGGGTGCCAGTTCCACCCGGTTTTACAATCACAACCGAAGCGTGCAATGCCTATTACGAAA
ACGGCCAGCAGTTCCCCGAAGGCATGTGGGAACAGGTACTGGAAGGCTTGAAAGATATCGAGGCGCAAACCGGCAAACGG
TTTGGCGATCCGTCCAACCCTCTCCTGGTCTCGGTGCGGTCAGGCGCCAAGTTCTCGATGCCGGGTATGATGGATACGGT
ACTCAACCTCGGTCTAAACCAGCAGACTCGCGAAGGGCTGGCGCGCCTCACCAATAATCCCCGGTTCGCCGCCGATGCCT
ACCGCCGCTTTGTCCAGCTCTTCGGCAAGATTGTGTTGGGTGTTGACGGTGATCGCTTCGAGCACGAGATGGACGAAGCG
AAAGGTCACGGTCAACGCCAGGACACCGATCTAACCACCGAAGAGTTGTTGGCGCTGGCCGAGAAGTTCAAAGCGATCAT
CAAGGAAGATGTTGGTGTCGATTTTCCCGAAGACCCCTTCGAGCAGTTGCGCGCAGCGATTGCCGCCGTGTTCCGCTCAT
GGAATGGACGGCGCGCTGTCGATTACCGCCGCGTTAACAAGATTCCCGATAATCTCGGCACGGCAGTCAATGTCCAGGCC
ATGGTCTTCGGCAATATGGGGAATGACTCGGCAACCGGCGTTGCCTTCACCCGCAATCCCAGCACCGGCGAGCCGGAAAT
TTTCGGTGAATATCTGGTAAATGCCCAGGGTGAAGATGTCGTCGCCGGTATTCGCACACCACAGCCGATCAGCAAACTGG
CCCAAGAGATGCCTGAAGTCTACGAGCAATTCCATCAGATTGCCAAGCAGCTCGAACGGCATTACAAAGACATGCAGGAT
GTCGAATTTACTATCGAGCGTGGCAAGCTATGGATGTTGCAAACCCGTACCGGCAAACGCACCGGCGCGGCTGCGGTGAA
GATCGCCGTTGATATGGTCAACGAGGGCTTGATCGACCGGGCAACTGCTGTTCAGCGGGTTGATCCGCATGCACTCGATC
AATTGCTCCACCCCATGATCGATCCTGCGGCTCGCAAGGACGCCCATCCTCTGACTGTTGGCCTACCGGCCTCACCCGGT
GCTGCGTCCGGTAAAGTGGTTTTCGATCCCGACGAAGCCGAGGAGCTGGCCAAAACCGGCGAAAAGGTGATCCTGGTGCG
CGTCGAGACTGCACCCGAAGATTTTCACGGCATGGTGGCCGCGCAGGCTATCCTGACCGCCCGCGGCGGCATGACGTCGC
ACGCTGCCGTGGTTGCCCGTGGCATGGGCAAACCGTGCGTTGCGGGTGCCGGTGATTTGCGCATTAGCTACGCCGACCAG
TTAATGACCGTGAATGGGACAACGATCCGCAAAGGCGATTGGATTACCCTCGACGGCGGTAGTGGTGAGGTCTTCGCCGG
CAAGATGCCGACGGTGGAACCCGAACTTTCGGGCGATTTTGCCACCTTGATGTCGTGGGCCGATGAATTCCGCAAGCTCG
GTGTGCGGGCCAATGCCGATATTCCGCGCGACGCCCGTGTTGCCCGTCAGTTCGGTGCCGAAGGGATCGGTCTCTGCCGG
ACTGAGCACATGTTCTTTGAAGGTGACCGGATCGATGCCATGCGCGAGATGATCCTGGCCGACACGGTAGAAGAGCGTCG
GGCTGCGTTGGCCAAGATCGAACCGCTTCAGCAGGGCGACTTCGAGGGTCTGTTCCGCGAAATGGCCGGTCTACCGGTGA
CGATTCGCACCCTCGACCCACCGCTGCACGAATTCCTACCCCATGATGACGAAGAGATCGAGGCGCTGGCCAACAAGCTA
GGCGTCGATCCGCGCAAGGTCAAGGCCCGGGTTGAAAGCCTGCGCGAGGCAAACCCGATGCTGGGTTTCCGTGGTTGTCG
GTTGGGCATCCTCTACCCCGAAATCACCGAAATGCAGGCACGGGCGATCTTCAAGGCGGCTGTCAAAGTCAAGGCGGAAG
GGATCGATGTCCATCCCGAAGTTATGATCCCACTGGTCGGCCATGTGAGCGAGTTGAAGCTGCAAGAGGAGATCGTGCGT
CGGGTTGCCGCCGAAGTCTTTGCCGAAGCCGGGATTGAAGTTCCCTACCTGGTTGGCACGATGATCGAGCTGCCGCGGGC
TGCGCTGGTGGCTGATGAAATCGCTCAGGTGGCAGAATTCTTCAGCTTTGGCACCAACGACCTGACCCAAACTACGCTCG
GTCTTTCACGAGACGACTCAGGGCGCTTTTTGCCGATCTATGTCGAAAAGAAGCTGATCCCCGATGATCCGTTCCAGACC
ATTGATCAGCGTGGGGTCGGGCAACTGGTGCAGATCGGTACCGAACGGGGCCGGTCAACCCGACCTGATCTGAAGGTCGG
TGTCTGTGGTGAGCACGGCGGTGATCCGGCCAGTGTCGAGTTCTTCCATCGGGTTGGCCTGAACTACGTCAGTTGTTCAC
CGTATCGGGTGCCGATTGCTCGACTGGCGGCTGCCCAGGCCGCACTCGGTGAAGCAAAGCGCGACAAGTAA

Upstream 100 bases:

>100_bases
GGGTTTTTGTGTGTTACGCAAGTCGCGTAGCCCGGCATCACTATCACTGCAAGCTCACCAGGACTTCACAAAGCTGCCTT
GTTCAAGGAGGAAACAGGTT

Downstream 100 bases:

>100_bases
CGGGACTGTTGCGCCCTCTTTAACCCCCACAGAACGGCGAGGCTCGCGAGAGCTTCGCCGTTCGTCGTGATCTTGTAACT
CACGATGATGACTGCCCTCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MTKKWVYLFREGNASMRDLLGGKGAGVAEMTRTGVPVPPGFTITTEACNAYYENGQQFPEGMWEQVLEGLKDIEAQTGKR
FGDPSNPLLVSVRSGAKFSMPGMMDTVLNLGLNQQTREGLARLTNNPRFAADAYRRFVQLFGKIVLGVDGDRFEHEMDEA
KGHGQRQDTDLTTEELLALAEKFKAIIKEDVGVDFPEDPFEQLRAAIAAVFRSWNGRRAVDYRRVNKIPDNLGTAVNVQA
MVFGNMGNDSATGVAFTRNPSTGEPEIFGEYLVNAQGEDVVAGIRTPQPISKLAQEMPEVYEQFHQIAKQLERHYKDMQD
VEFTIERGKLWMLQTRTGKRTGAAAVKIAVDMVNEGLIDRATAVQRVDPHALDQLLHPMIDPAARKDAHPLTVGLPASPG
AASGKVVFDPDEAEELAKTGEKVILVRVETAPEDFHGMVAAQAILTARGGMTSHAAVVARGMGKPCVAGAGDLRISYADQ
LMTVNGTTIRKGDWITLDGGSGEVFAGKMPTVEPELSGDFATLMSWADEFRKLGVRANADIPRDARVARQFGAEGIGLCR
TEHMFFEGDRIDAMREMILADTVEERRAALAKIEPLQQGDFEGLFREMAGLPVTIRTLDPPLHEFLPHDDEEIEALANKL
GVDPRKVKARVESLREANPMLGFRGCRLGILYPEITEMQARAIFKAAVKVKAEGIDVHPEVMIPLVGHVSELKLQEEIVR
RVAAEVFAEAGIEVPYLVGTMIELPRAALVADEIAQVAEFFSFGTNDLTQTTLGLSRDDSGRFLPIYVEKKLIPDDPFQT
IDQRGVGQLVQIGTERGRSTRPDLKVGVCGEHGGDPASVEFFHRVGLNYVSCSPYRVPIARLAAAQAALGEAKRDK

Sequences:

>Translated_876_residues
MTKKWVYLFREGNASMRDLLGGKGAGVAEMTRTGVPVPPGFTITTEACNAYYENGQQFPEGMWEQVLEGLKDIEAQTGKR
FGDPSNPLLVSVRSGAKFSMPGMMDTVLNLGLNQQTREGLARLTNNPRFAADAYRRFVQLFGKIVLGVDGDRFEHEMDEA
KGHGQRQDTDLTTEELLALAEKFKAIIKEDVGVDFPEDPFEQLRAAIAAVFRSWNGRRAVDYRRVNKIPDNLGTAVNVQA
MVFGNMGNDSATGVAFTRNPSTGEPEIFGEYLVNAQGEDVVAGIRTPQPISKLAQEMPEVYEQFHQIAKQLERHYKDMQD
VEFTIERGKLWMLQTRTGKRTGAAAVKIAVDMVNEGLIDRATAVQRVDPHALDQLLHPMIDPAARKDAHPLTVGLPASPG
AASGKVVFDPDEAEELAKTGEKVILVRVETAPEDFHGMVAAQAILTARGGMTSHAAVVARGMGKPCVAGAGDLRISYADQ
LMTVNGTTIRKGDWITLDGGSGEVFAGKMPTVEPELSGDFATLMSWADEFRKLGVRANADIPRDARVARQFGAEGIGLCR
TEHMFFEGDRIDAMREMILADTVEERRAALAKIEPLQQGDFEGLFREMAGLPVTIRTLDPPLHEFLPHDDEEIEALANKL
GVDPRKVKARVESLREANPMLGFRGCRLGILYPEITEMQARAIFKAAVKVKAEGIDVHPEVMIPLVGHVSELKLQEEIVR
RVAAEVFAEAGIEVPYLVGTMIELPRAALVADEIAQVAEFFSFGTNDLTQTTLGLSRDDSGRFLPIYVEKKLIPDDPFQT
IDQRGVGQLVQIGTERGRSTRPDLKVGVCGEHGGDPASVEFFHRVGLNYVSCSPYRVPIARLAAAQAALGEAKRDK
>Mature_875_residues
TKKWVYLFREGNASMRDLLGGKGAGVAEMTRTGVPVPPGFTITTEACNAYYENGQQFPEGMWEQVLEGLKDIEAQTGKRF
GDPSNPLLVSVRSGAKFSMPGMMDTVLNLGLNQQTREGLARLTNNPRFAADAYRRFVQLFGKIVLGVDGDRFEHEMDEAK
GHGQRQDTDLTTEELLALAEKFKAIIKEDVGVDFPEDPFEQLRAAIAAVFRSWNGRRAVDYRRVNKIPDNLGTAVNVQAM
VFGNMGNDSATGVAFTRNPSTGEPEIFGEYLVNAQGEDVVAGIRTPQPISKLAQEMPEVYEQFHQIAKQLERHYKDMQDV
EFTIERGKLWMLQTRTGKRTGAAAVKIAVDMVNEGLIDRATAVQRVDPHALDQLLHPMIDPAARKDAHPLTVGLPASPGA
ASGKVVFDPDEAEELAKTGEKVILVRVETAPEDFHGMVAAQAILTARGGMTSHAAVVARGMGKPCVAGAGDLRISYADQL
MTVNGTTIRKGDWITLDGGSGEVFAGKMPTVEPELSGDFATLMSWADEFRKLGVRANADIPRDARVARQFGAEGIGLCRT
EHMFFEGDRIDAMREMILADTVEERRAALAKIEPLQQGDFEGLFREMAGLPVTIRTLDPPLHEFLPHDDEEIEALANKLG
VDPRKVKARVESLREANPMLGFRGCRLGILYPEITEMQARAIFKAAVKVKAEGIDVHPEVMIPLVGHVSELKLQEEIVRR
VAAEVFAEAGIEVPYLVGTMIELPRAALVADEIAQVAEFFSFGTNDLTQTTLGLSRDDSGRFLPIYVEKKLIPDDPFQTI
DQRGVGQLVQIGTERGRSTRPDLKVGVCGEHGGDPASVEFFHRVGLNYVSCSPYRVPIARLAAAQAALGEAKRDK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=460, Percent_Identity=24.5652173913043, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1787994, Length=926, Percent_Identity=23.5421166306695, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789193, Length=431, Percent_Identity=25.2900232018561, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788726, Length=453, Percent_Identity=22.7373068432671, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI48994992, Length=442, Percent_Identity=23.7556561085973, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96017; Mature: 95886

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKWVYLFREGNASMRDLLGGKGAGVAEMTRTGVPVPPGFTITTEACNAYYENGQQFPE
CCCCEEEEEECCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCHH
GMWEQVLEGLKDIEAQTGKRFGDPSNPLLVSVRSGAKFSMPGMMDTVLNLGLNQQTREGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
ARLTNNPRFAADAYRRFVQLFGKIVLGVDGDRFEHEMDEAKGHGQRQDTDLTTEELLALA
HHHCCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKFKAIIKEDVGVDFPEDPFEQLRAAIAAVFRSWNGRRAVDYRRVNKIPDNLGTAVNVQA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHCCEEEEEE
MVFGNMGNDSATGVAFTRNPSTGEPEIFGEYLVNAQGEDVVAGIRTPQPISKLAQEMPEV
EEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHH
YEQFHQIAKQLERHYKDMQDVEFTIERGKLWMLQTRTGKRTGAAAVKIAVDMVNEGLIDR
HHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHH
ATAVQRVDPHALDQLLHPMIDPAARKDAHPLTVGLPASPGAASGKVVFDPDEAEELAKTG
HHHHHHCCHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHCC
EKVILVRVETAPEDFHGMVAAQAILTARGGMTSHAAVVARGMGKPCVAGAGDLRISYADQ
CEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCC
LMTVNGTTIRKGDWITLDGGSGEVFAGKMPTVEPELSGDFATLMSWADEFRKLGVRANAD
EEEECCCEEECCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
IPRDARVARQFGAEGIGLCRTEHMFFEGDRIDAMREMILADTVEERRAALAKIEPLQQGD
CCHHHHHHHHHCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FEGLFREMAGLPVTIRTLDPPLHEFLPHDDEEIEALANKLGVDPRKVKARVESLREANPM
HHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LGFRGCRLGILYPEITEMQARAIFKAAVKVKAEGIDVHPEVMIPLVGHVSELKLQEEIVR
CCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHH
RVAAEVFAEAGIEVPYLVGTMIELPRAALVADEIAQVAEFFSFGTNDLTQTTLGLSRDDS
HHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
GRFLPIYVEKKLIPDDPFQTIDQRGVGQLVQIGTERGRSTRPDLKVGVCGEHGGDPASVE
CCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHH
FFHRVGLNYVSCSPYRVPIARLAAAQAALGEAKRDK
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKWVYLFREGNASMRDLLGGKGAGVAEMTRTGVPVPPGFTITTEACNAYYENGQQFPE
CCCEEEEEECCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCHH
GMWEQVLEGLKDIEAQTGKRFGDPSNPLLVSVRSGAKFSMPGMMDTVLNLGLNQQTREGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHH
ARLTNNPRFAADAYRRFVQLFGKIVLGVDGDRFEHEMDEAKGHGQRQDTDLTTEELLALA
HHHCCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EKFKAIIKEDVGVDFPEDPFEQLRAAIAAVFRSWNGRRAVDYRRVNKIPDNLGTAVNVQA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHCCEEEEEE
MVFGNMGNDSATGVAFTRNPSTGEPEIFGEYLVNAQGEDVVAGIRTPQPISKLAQEMPEV
EEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHH
YEQFHQIAKQLERHYKDMQDVEFTIERGKLWMLQTRTGKRTGAAAVKIAVDMVNEGLIDR
HHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHH
ATAVQRVDPHALDQLLHPMIDPAARKDAHPLTVGLPASPGAASGKVVFDPDEAEELAKTG
HHHHHHCCHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHCC
EKVILVRVETAPEDFHGMVAAQAILTARGGMTSHAAVVARGMGKPCVAGAGDLRISYADQ
CEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCC
LMTVNGTTIRKGDWITLDGGSGEVFAGKMPTVEPELSGDFATLMSWADEFRKLGVRANAD
EEEECCCEEECCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
IPRDARVARQFGAEGIGLCRTEHMFFEGDRIDAMREMILADTVEERRAALAKIEPLQQGD
CCHHHHHHHHHCCCCCCEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FEGLFREMAGLPVTIRTLDPPLHEFLPHDDEEIEALANKLGVDPRKVKARVESLREANPM
HHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LGFRGCRLGILYPEITEMQARAIFKAAVKVKAEGIDVHPEVMIPLVGHVSELKLQEEIVR
CCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHH
RVAAEVFAEAGIEVPYLVGTMIELPRAALVADEIAQVAEFFSFGTNDLTQTTLGLSRDDS
HHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
GRFLPIYVEKKLIPDDPFQTIDQRGVGQLVQIGTERGRSTRPDLKVGVCGEHGGDPASVE
CCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHH
FFHRVGLNYVSCSPYRVPIARLAAAQAALGEAKRDK
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]