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The map label for this gene is dnaG [H]

Identifier: 222526386

GI number: 222526386

Start: 3904532

End: 3906499

Strand: Reverse

Name: dnaG [H]

Synonym: Chy400_3152

Alternate gene names: 222526386

Gene position: 3906499-3904532 (Counterclockwise)

Preceding gene: 222526387

Following gene: 222526383

Centisome position: 74.14

GC content: 57.62

Gene sequence:

>1968_bases
ATGAGCGTTATTGATGATATTAAAGCCAATATCGACATCGTTGACCTGATCAACTCGATGGGGGTTGGCCTGCGACGCAG
CGGGCGCAGTTTTGTCGGTTTCTGTCCGTTCCATCCCAATACGCGCACACCGGCCTTTCACGTCTACCCCGACACCCAGA
GTTTCTATTGCTTCGGCTGCCACGCCTCCGGTACCGCCTTCGATTTTGTGATGCGTAAGCAGGGGTTGGACTTCAAGGGG
GCGTTGGAGCTGCTGGCTGCAAGGGCCGGGATCAGGCTTGAACCGAAGAATGATGCCCAGCGCCAGGAAGATGCGCGGCG
GGCACGGCTGCTTGAGATTAATGGTATCGCCGCTCGCTACTTCAACTATGTGCTGCTCAATCTCAGTCGCGGTGAACCTG
GCCGTGATTACATCGCCAGGCGCGGGATTAACCAGGACGCAATTGATGCCTTTCAGATCGGCTATAGCCTCGATGACTGG
AATCATCTCTTTTCCTATCTGCACGAAAAGAAGGGCTACAGTGTCGAAGACCTGATCACTGCCGGGTTGGTTATTCCCGG
TGAACGTGGCCCCTACGACCGCTTTCGCAACCGGATTATCTTTCCGATCCGTAATATTCGCGGTGAGGTGATTGGCTTTG
GCGGTCGGGCACTGGGTGACGCTCAACCCAAATATCTCAACACTCCAGAAACCCCGCTGTTCAAGAAGAGTGAGGTGCTA
TACGGGTTAGACCTGGCCCGCGACGCGATTCGGACGGCCAACCGGGCGATTGTGGTTGAGGGATATGTTGATGTGATTAC
CGCCCACCAGCACGGCTTTCGTAATGTGGTGGCACCCCTGGGAACAGCCCTGAGCAAAGCTCACATCAACCAGCTTAAAC
GGCTCACCGATCAGGTCTACCTGGCGCTTGACGCCGATGCTGCCGGTCAGAAAGCAACCCTTCGCGGTCTGGAAACCATC
CGCACGACGAGCGAAGAGGAAGGCGAAGGTCGCATGGTGACCACGGCGCAGGGGATCGTTCGCCTGGAGAACGATGTGAC
CATCCGGATCATTCGCTTGCCGGCGGGACGTGATCCCGACGAGGTGATTGCTGCCGATCCCCAGCTCTGGCAAACCCTGG
TCGATCAGGCCGCACCGGTGATGGATTTCTACCTTGAGGCATACACCGCCGGTCTCGACATGCACGATCCGACCCAACAA
CGGCAGGCGCTTGAGCGAATCATTCCCCTCCTGAGTGAGCTTGACGGCGCTGCCCAGCGGGTCTACGTTGCCCGGGTCGA
ACAGCTTACCGGTGTCCGCAGCGAGTTAATCGTTGATCTCATTCGGACCCGTCTGCAACCAACGCGACGGAGCGAGCGGA
ACCGCACATCACGGCCATCAGCACCACCGTTACCGGCAACATCTCCGGTAAGCACCACACCGCTCGATGAACACCGTCGC
CATACCGAAGCCTACTTGCTGGCCCTGGCCCTGCGCTATCCCTCGGTGAATCTGGCCATCGAAAATCTGCTGGAGAACCT
CAAAAAACAGCACCGCGACGCCGGTGCGCTCTTCGGGGCCGGTTTGGAAGACCTGCTTGAAGAACCGCTGCATCAGGCGA
TCTGGCAGGCGTATCTGGCCACACCGCTTGAGTTGCGGCCAACCGACCCAGAGAGTTTGCAATTCTGGGTAGACACGCTC
GGCGAGCCATTAAGCAGCGAGGCCAAAGCCCTGCTCGAGGTTCTGAACCGTCGCCCTGACGATGTGCGTTTCAGGCACGA
AGCCGAACAATGTGCCCGTCTGATCCGCAAGGCGCAAATTATGGCCCGCATCCGTCGCTACAAAGAGCGACTGGATGAAT
TGACTGATGAAGAAGAAATCCAACAGGTTTTGCAGCACATTCGCGATCTGAGCAGCTACGCCGAACAGATCACACGTCCA
CGCCAATCGAGCACGTTTCCCGATTTACGCAATCTGCTCGGCCAGTGA

Upstream 100 bases:

>100_bases
GATCGCTATGCGCTCGATACATTCAACCGCATTTTTGTCCCGCGTCTGTGGCACGTGCTGTAACGCCTCTGTGCAGTAAT
CTGCCGGGTTGAGGAAGGTT

Downstream 100 bases:

>100_bases
GGAGGGAGAAGATAGGAGGGAGAAGATAGGAGCGAGAAAATAGGAACGAGAAAATAGGAGCGAGAAAATAGGAGCGAGAA
AATAGGAGCGAGAAAATAGG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSVIDDIKANIDIVDLINSMGVGLRRSGRSFVGFCPFHPNTRTPAFHVYPDTQSFYCFGCHASGTAFDFVMRKQGLDFKG
ALELLAARAGIRLEPKNDAQRQEDARRARLLEINGIAARYFNYVLLNLSRGEPGRDYIARRGINQDAIDAFQIGYSLDDW
NHLFSYLHEKKGYSVEDLITAGLVIPGERGPYDRFRNRIIFPIRNIRGEVIGFGGRALGDAQPKYLNTPETPLFKKSEVL
YGLDLARDAIRTANRAIVVEGYVDVITAHQHGFRNVVAPLGTALSKAHINQLKRLTDQVYLALDADAAGQKATLRGLETI
RTTSEEEGEGRMVTTAQGIVRLENDVTIRIIRLPAGRDPDEVIAADPQLWQTLVDQAAPVMDFYLEAYTAGLDMHDPTQQ
RQALERIIPLLSELDGAAQRVYVARVEQLTGVRSELIVDLIRTRLQPTRRSERNRTSRPSAPPLPATSPVSTTPLDEHRR
HTEAYLLALALRYPSVNLAIENLLENLKKQHRDAGALFGAGLEDLLEEPLHQAIWQAYLATPLELRPTDPESLQFWVDTL
GEPLSSEAKALLEVLNRRPDDVRFRHEAEQCARLIRKAQIMARIRRYKERLDELTDEEEIQQVLQHIRDLSSYAEQITRP
RQSSTFPDLRNLLGQ

Sequences:

>Translated_655_residues
MSVIDDIKANIDIVDLINSMGVGLRRSGRSFVGFCPFHPNTRTPAFHVYPDTQSFYCFGCHASGTAFDFVMRKQGLDFKG
ALELLAARAGIRLEPKNDAQRQEDARRARLLEINGIAARYFNYVLLNLSRGEPGRDYIARRGINQDAIDAFQIGYSLDDW
NHLFSYLHEKKGYSVEDLITAGLVIPGERGPYDRFRNRIIFPIRNIRGEVIGFGGRALGDAQPKYLNTPETPLFKKSEVL
YGLDLARDAIRTANRAIVVEGYVDVITAHQHGFRNVVAPLGTALSKAHINQLKRLTDQVYLALDADAAGQKATLRGLETI
RTTSEEEGEGRMVTTAQGIVRLENDVTIRIIRLPAGRDPDEVIAADPQLWQTLVDQAAPVMDFYLEAYTAGLDMHDPTQQ
RQALERIIPLLSELDGAAQRVYVARVEQLTGVRSELIVDLIRTRLQPTRRSERNRTSRPSAPPLPATSPVSTTPLDEHRR
HTEAYLLALALRYPSVNLAIENLLENLKKQHRDAGALFGAGLEDLLEEPLHQAIWQAYLATPLELRPTDPESLQFWVDTL
GEPLSSEAKALLEVLNRRPDDVRFRHEAEQCARLIRKAQIMARIRRYKERLDELTDEEEIQQVLQHIRDLSSYAEQITRP
RQSSTFPDLRNLLGQ
>Mature_654_residues
SVIDDIKANIDIVDLINSMGVGLRRSGRSFVGFCPFHPNTRTPAFHVYPDTQSFYCFGCHASGTAFDFVMRKQGLDFKGA
LELLAARAGIRLEPKNDAQRQEDARRARLLEINGIAARYFNYVLLNLSRGEPGRDYIARRGINQDAIDAFQIGYSLDDWN
HLFSYLHEKKGYSVEDLITAGLVIPGERGPYDRFRNRIIFPIRNIRGEVIGFGGRALGDAQPKYLNTPETPLFKKSEVLY
GLDLARDAIRTANRAIVVEGYVDVITAHQHGFRNVVAPLGTALSKAHINQLKRLTDQVYLALDADAAGQKATLRGLETIR
TTSEEEGEGRMVTTAQGIVRLENDVTIRIIRLPAGRDPDEVIAADPQLWQTLVDQAAPVMDFYLEAYTAGLDMHDPTQQR
QALERIIPLLSELDGAAQRVYVARVEQLTGVRSELIVDLIRTRLQPTRRSERNRTSRPSAPPLPATSPVSTTPLDEHRRH
TEAYLLALALRYPSVNLAIENLLENLKKQHRDAGALFGAGLEDLLEEPLHQAIWQAYLATPLELRPTDPESLQFWVDTLG
EPLSSEAKALLEVLNRRPDDVRFRHEAEQCARLIRKAQIMARIRRYKERLDELTDEEEIQQVLQHIRDLSSYAEQITRPR
QSSTFPDLRNLLGQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=534, Percent_Identity=32.2097378277154, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 73932; Mature: 73801

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIDDIKANIDIVDLINSMGVGLRRSGRSFVGFCPFHPNTRTPAFHVYPDTQSFYCFGC
CCCHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCEEEEEE
HASGTAFDFVMRKQGLDFKGALELLAARAGIRLEPKNDAQRQEDARRARLLEINGIAARY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHEECHHHHHH
FNYVLLNLSRGEPGRDYIARRGINQDAIDAFQIGYSLDDWNHLFSYLHEKKGYSVEDLIT
HHHHEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHH
AGLVIPGERGPYDRFRNRIIFPIRNIRGEVIGFGGRALGDAQPKYLNTPETPLFKKSEVL
HCEEECCCCCCHHHHCCCEEEEHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
YGLDLARDAIRTANRAIVVEGYVDVITAHQHGFRNVVAPLGTALSKAHINQLKRLTDQVY
HHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LALDADAAGQKATLRGLETIRTTSEEEGEGRMVTTAQGIVRLENDVTIRIIRLPAGRDPD
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCEEEEEEECCCCCCHH
EVIAADPQLWQTLVDQAAPVMDFYLEAYTAGLDMHDPTQQRQALERIIPLLSELDGAAQR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
VYVARVEQLTGVRSELIVDLIRTRLQPTRRSERNRTSRPSAPPLPATSPVSTTPLDEHRR
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
HTEAYLLALALRYPSVNLAIENLLENLKKQHRDAGALFGAGLEDLLEEPLHQAIWQAYLA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
TPLELRPTDPESLQFWVDTLGEPLSSEAKALLEVLNRRPDDVRFRHEAEQCARLIRKAQI
CCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MARIRRYKERLDELTDEEEIQQVLQHIRDLSSYAEQITRPRQSSTFPDLRNLLGQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SVIDDIKANIDIVDLINSMGVGLRRSGRSFVGFCPFHPNTRTPAFHVYPDTQSFYCFGC
CCHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCEEEEEE
HASGTAFDFVMRKQGLDFKGALELLAARAGIRLEPKNDAQRQEDARRARLLEINGIAARY
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHEECHHHHHH
FNYVLLNLSRGEPGRDYIARRGINQDAIDAFQIGYSLDDWNHLFSYLHEKKGYSVEDLIT
HHHHEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHH
AGLVIPGERGPYDRFRNRIIFPIRNIRGEVIGFGGRALGDAQPKYLNTPETPLFKKSEVL
HCEEECCCCCCHHHHCCCEEEEHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
YGLDLARDAIRTANRAIVVEGYVDVITAHQHGFRNVVAPLGTALSKAHINQLKRLTDQVY
HHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LALDADAAGQKATLRGLETIRTTSEEEGEGRMVTTAQGIVRLENDVTIRIIRLPAGRDPD
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEECCCEEEEEEECCCCCCHH
EVIAADPQLWQTLVDQAAPVMDFYLEAYTAGLDMHDPTQQRQALERIIPLLSELDGAAQR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
VYVARVEQLTGVRSELIVDLIRTRLQPTRRSERNRTSRPSAPPLPATSPVSTTPLDEHRR
HHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
HTEAYLLALALRYPSVNLAIENLLENLKKQHRDAGALFGAGLEDLLEEPLHQAIWQAYLA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
TPLELRPTDPESLQFWVDTLGEPLSSEAKALLEVLNRRPDDVRFRHEAEQCARLIRKAQI
CCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MARIRRYKERLDELTDEEEIQQVLQHIRDLSSYAEQITRPRQSSTFPDLRNLLGQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]