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The map label for this gene is fabI [H]

Identifier: 222526383

GI number: 222526383

Start: 3895084

End: 3895866

Strand: Reverse

Name: fabI [H]

Synonym: Chy400_3149

Alternate gene names: 222526383

Gene position: 3895866-3895084 (Counterclockwise)

Preceding gene: 222526386

Following gene: 222526382

Centisome position: 73.94

GC content: 57.6

Gene sequence:

>783_bases
ATGGGCCTGATGGAGGGAAAAAAGGGCCTGATCCTTGGCGTAGCCAATGATCGCTCGATTGCCTGGGGGATTGCGCAGGC
ACTACACCGCGAGGGAGCTACTATCGGTTTCACGTACCTTGGTGAAGCACTTGAACGGCGGGTCCGTCCGCTGGCGGAAA
GCCTCAACTCGCCGTTGATCGTGCCCTGTGACGTGTCCAAAGATGAAGACATTACCGCCTTGATCGAACAGGCACGTGAA
ACATTCGGACAGATTGATTTTCTCGTCCACGCTATTGCCTTCGCCAACAAAGAAGAGCTGAGTGGCACAATCCTGAACAC
AACCCGCGAAGGATTCCGCATCGCCCTTGAGATCAGCGCATACTCACTGATCGCGCTGGTCAAGGCCGCCGAACCGATCT
TTGCCCCTGACGCCAGCGTCCTGACATTAACCTACCACGGTGCACGCCAGGTGATCGGCAGCTACAATGTGATGGGTGTC
GCCAAAGCTGCGCTGGAAGCCAGCGTGCGCTATCTCGCTGCCGGTCTTGGCCCCCGTGGGATCAGAGTCAACGCGATCAG
CGCCGGCCCGATTCGCACCCTGGCTGCCAGTGGCATTGCCAATTTCCGCAGCCTGCACAAACATTTCGCCGAATATGCTC
CCCTGCGGCGCAATGTCACGATTGAAGATGTAGGAAATGCCGCCCTGTATCTCTGCTCACCGCTCGCAGCCGGCGTAACC
GGCGAAATCCATTATGTTGATGCCGGCTTTAATGTGGTCGTGCCCGGTAGCGGTGAAGAGTAG

Upstream 100 bases:

>100_bases
GCTATCCGCCAAAGTATGGTAAGATGGCGCGCTAGTGAGGCAACGCAACGTTTGCTCAGGAAAGCATGCTGCCAGACAGC
ACAATAATGGAGGATGTACC

Downstream 100 bases:

>100_bases
CAGATCACCCGCCGGTATGCTACAATGAACGCAATGCGCGCACGACAATGCGGAGGAGAGCATGGTTGAGGTTGCAGTGG
TATCGTCCGATAATACGACC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGLMEGKKGLILGVANDRSIAWGIAQALHREGATIGFTYLGEALERRVRPLAESLNSPLIVPCDVSKDEDITALIEQARE
TFGQIDFLVHAIAFANKEELSGTILNTTREGFRIALEISAYSLIALVKAAEPIFAPDASVLTLTYHGARQVIGSYNVMGV
AKAALEASVRYLAAGLGPRGIRVNAISAGPIRTLAASGIANFRSLHKHFAEYAPLRRNVTIEDVGNAALYLCSPLAAGVT
GEIHYVDAGFNVVVPGSGEE

Sequences:

>Translated_260_residues
MGLMEGKKGLILGVANDRSIAWGIAQALHREGATIGFTYLGEALERRVRPLAESLNSPLIVPCDVSKDEDITALIEQARE
TFGQIDFLVHAIAFANKEELSGTILNTTREGFRIALEISAYSLIALVKAAEPIFAPDASVLTLTYHGARQVIGSYNVMGV
AKAALEASVRYLAAGLGPRGIRVNAISAGPIRTLAASGIANFRSLHKHFAEYAPLRRNVTIEDVGNAALYLCSPLAAGVT
GEIHYVDAGFNVVVPGSGEE
>Mature_259_residues
GLMEGKKGLILGVANDRSIAWGIAQALHREGATIGFTYLGEALERRVRPLAESLNSPLIVPCDVSKDEDITALIEQARET
FGQIDFLVHAIAFANKEELSGTILNTTREGFRIALEISAYSLIALVKAAEPIFAPDASVLTLTYHGARQVIGSYNVMGVA
KAALEASVRYLAAGLGPRGIRVNAISAGPIRTLAASGIANFRSLHKHFAEYAPLRRNVTIEDVGNAALYLCSPLAAGVTG
EIHYVDAGFNVVVPGSGEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=226, Percent_Identity=31.4159292035398, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI10190704, Length=226, Percent_Identity=28.7610619469027, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=50.9881422924901, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1787905, Length=189, Percent_Identity=29.1005291005291, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=27.9527559055118, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=26.7441860465116, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=216, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI23397609, Length=243, Percent_Identity=29.2181069958848, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27614; Mature: 27483

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLMEGKKGLILGVANDRSIAWGIAQALHREGATIGFTYLGEALERRVRPLAESLNSPLI
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEE
VPCDVSKDEDITALIEQARETFGQIDFLVHAIAFANKEELSGTILNTTREGFRIALEISA
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCEEEEEEEHH
YSLIALVKAAEPIFAPDASVLTLTYHGARQVIGSYNVMGVAKAALEASVRYLAAGLGPRG
HHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IRVNAISAGPIRTLAASGIANFRSLHKHFAEYAPLRRNVTIEDVGNAALYLCSPLAAGVT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCEEEHHHHHCCCC
GEIHYVDAGFNVVVPGSGEE
CEEEEEECCCEEEECCCCCC
>Mature Secondary Structure 
GLMEGKKGLILGVANDRSIAWGIAQALHREGATIGFTYLGEALERRVRPLAESLNSPLI
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCEE
VPCDVSKDEDITALIEQARETFGQIDFLVHAIAFANKEELSGTILNTTREGFRIALEISA
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCEEEEEEEHH
YSLIALVKAAEPIFAPDASVLTLTYHGARQVIGSYNVMGVAKAALEASVRYLAAGLGPRG
HHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IRVNAISAGPIRTLAASGIANFRSLHKHFAEYAPLRRNVTIEDVGNAALYLCSPLAAGVT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCEEEHHHHHCCCC
GEIHYVDAGFNVVVPGSGEE
CEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]