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The map label for this gene is yieO [C]

Identifier: 222526332

GI number: 222526332

Start: 3822724

End: 3824256

Strand: Direct

Name: yieO [C]

Synonym: Chy400_3098

Alternate gene names: 222526332

Gene position: 3822724-3824256 (Clockwise)

Preceding gene: 222526331

Following gene: 222526333

Centisome position: 72.55

GC content: 61.25

Gene sequence:

>1533_bases
ATGAAAAATCCTGCCTTGACAACGGTTGAAACCCGTCCGGTCGATCCACGGCTGACCCTGGGGCTGGTCTGTCTGGCAAT
CTTCATCGGCGCAGTTGACCTGACGGTGATCAGTGCTGCGTTACCGAAGGTCATGATTGACCTGCGTCTTTCCCTGGATA
CGGAGTTGAATCGGGCTTCCTGGGCGGTGAGTGGCTATTTGCTGGCCTACACGGTGAGTATGACCTTTATGGGTCGGCTG
TCTGATCTGTTTGGCCGGCGAATGGTGTATCTGATCTGCCTGGTCGTTTTCCTCATCGGTTCAGCCTGGGTGGCAGCCGC
GCCGAATCTGACGATGCTGATTATCGGACGGGTGATTCAGGCGTTTGGGGCCGGAGCGATGGTGCCGGTCTCGATGGCCC
TGGTTGGCGATCTCTTCCCGCCAGGACAACGGGCAGCCGCGCTCGGTCTGATCGGTGCCGTTGATACCGCCGGATGGATG
GTCGGGCATCTCTACGGCGGCGTGCTTATGCGCATCTTTGACGACTGGCGGCTCCTGTTTTGGCTCAACCTGCCTATTGG
TCTGGTCGCCCTGGCATTGACGTGGTATGCGCTGCGTCAGGTGCCGACCCCGCCGCGGGTCGGGCGTTTCGATTGGCCGG
GCACGCTGTTGTTGAGCGCTAGCCTCGTTGCGTTGAATATCGGTCTGGCTGCCGGGAGCGAACTAGGCGCGACCGATTTC
TACGGTGAGCGCCTGGGACCACCGCCCTACGCCGGGCCGCTGGTGATAGTGTCCCTGCTGTTGCTGGCACTCTTCGTTTG
GGTCGAGCGCCGTAGTGACGATCCGCTGATTGGCCTGGAACTGTTCACCCACCGTCATACCGCGATGGCCTGCGTGATCA
ACGTGATGGTTGGGTTCGGACTGGCAATTGCGATCACGAATGTACCTCTCTACATCAACACCCGTTTGTTGCTGTACCAT
CCGACCGATAGCGACATTCTCCGTATCGCCGCCTGGGATGCCGGCTGGATGCTATCAGCATTAACCCTGACAATGGCGGC
TGCTGCACTCCCCGGCGGACTGCTCACCGGGCGGTTTGGAGCACGGTTGCCGGCAATGTTGGGCTTAGGACTGGCTATCA
TTGGCTATGGCCTGATGGCGTTTTGGGGGCCGGACGCGACCTACCTGCGGATGGGTCTGGAACTGGCGTTGACCGGTATT
GGTCTGGGCCTGGTGATTGCGCCGGTCGCCGACACAGTGATTGCCGCTGCCGGTGAAGATCGTCGCGGTGCAGCCTCGGC
TCTGGTGATCGCCCTGCGTCTGGTGGGCATGACGGTGGGTGTAGCAATCCTCACCCTGTGGGGCGTTCATCGCCAGGACG
AGCTGCGGCGGGCTGGCGCTGACAACCCGCTCGCTGTCACCGAGCCGGCTCGTTTTCTGATGGAGATTGCGGCACGGGTC
ATCGGGGAGACATTTCTCTTCGGGGCAGCAGCATGCCTGGTTGGCCTCCTGGCCGCCCTGGTAATGCGAGAGGTATCCGG
GCGCCGAACATAG

Upstream 100 bases:

>100_bases
TGGTCGATACAGCCACTGACGCCAGTAGCCCCACCACCTGGACGCTTGAGTTCAGTGACTATGACAAAGCGGTTGATGTT
CGTGAACCGGTGCAATGTCC

Downstream 100 bases:

>100_bases
ATGTGAATTCGTGGTATCATCATCCCATGGTGCCATTTGCTCCGTAAGGACACACTCAATGCACGATGAAGAGATCCAAC
GTCTCATTAACCAGTATTTG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKNPALTTVETRPVDPRLTLGLVCLAIFIGAVDLTVISAALPKVMIDLRLSLDTELNRASWAVSGYLLAYTVSMTFMGRL
SDLFGRRMVYLICLVVFLIGSAWVAAAPNLTMLIIGRVIQAFGAGAMVPVSMALVGDLFPPGQRAAALGLIGAVDTAGWM
VGHLYGGVLMRIFDDWRLLFWLNLPIGLVALALTWYALRQVPTPPRVGRFDWPGTLLLSASLVALNIGLAAGSELGATDF
YGERLGPPPYAGPLVIVSLLLLALFVWVERRSDDPLIGLELFTHRHTAMACVINVMVGFGLAIAITNVPLYINTRLLLYH
PTDSDILRIAAWDAGWMLSALTLTMAAAALPGGLLTGRFGARLPAMLGLGLAIIGYGLMAFWGPDATYLRMGLELALTGI
GLGLVIAPVADTVIAAAGEDRRGAASALVIALRLVGMTVGVAILTLWGVHRQDELRRAGADNPLAVTEPARFLMEIAARV
IGETFLFGAAACLVGLLAALVMREVSGRRT

Sequences:

>Translated_510_residues
MKNPALTTVETRPVDPRLTLGLVCLAIFIGAVDLTVISAALPKVMIDLRLSLDTELNRASWAVSGYLLAYTVSMTFMGRL
SDLFGRRMVYLICLVVFLIGSAWVAAAPNLTMLIIGRVIQAFGAGAMVPVSMALVGDLFPPGQRAAALGLIGAVDTAGWM
VGHLYGGVLMRIFDDWRLLFWLNLPIGLVALALTWYALRQVPTPPRVGRFDWPGTLLLSASLVALNIGLAAGSELGATDF
YGERLGPPPYAGPLVIVSLLLLALFVWVERRSDDPLIGLELFTHRHTAMACVINVMVGFGLAIAITNVPLYINTRLLLYH
PTDSDILRIAAWDAGWMLSALTLTMAAAALPGGLLTGRFGARLPAMLGLGLAIIGYGLMAFWGPDATYLRMGLELALTGI
GLGLVIAPVADTVIAAAGEDRRGAASALVIALRLVGMTVGVAILTLWGVHRQDELRRAGADNPLAVTEPARFLMEIAARV
IGETFLFGAAACLVGLLAALVMREVSGRRT
>Mature_510_residues
MKNPALTTVETRPVDPRLTLGLVCLAIFIGAVDLTVISAALPKVMIDLRLSLDTELNRASWAVSGYLLAYTVSMTFMGRL
SDLFGRRMVYLICLVVFLIGSAWVAAAPNLTMLIIGRVIQAFGAGAMVPVSMALVGDLFPPGQRAAALGLIGAVDTAGWM
VGHLYGGVLMRIFDDWRLLFWLNLPIGLVALALTWYALRQVPTPPRVGRFDWPGTLLLSASLVALNIGLAAGSELGATDF
YGERLGPPPYAGPLVIVSLLLLALFVWVERRSDDPLIGLELFTHRHTAMACVINVMVGFGLAIAITNVPLYINTRLLLYH
PTDSDILRIAAWDAGWMLSALTLTMAAAALPGGLLTGRFGARLPAMLGLGLAIIGYGLMAFWGPDATYLRMGLELALTGI
GLGLVIAPVADTVIAAAGEDRRGAASALVIALRLVGMTVGVAILTLWGVHRQDELRRAGADNPLAVTEPARFLMEIAARV
IGETFLFGAAACLVGLLAALVMREVSGRRT

Specific function: Confers resistance to tetracycline and aminoglycosides such as streptomycin and gentamicin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=285, Percent_Identity=29.1228070175439, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=25.7075471698113, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=24.0362811791383, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789042, Length=443, Percent_Identity=23.7020316027088, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788392, Length=224, Percent_Identity=27.2321428571429, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=28.9156626506024, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=482, Percent_Identity=23.0290456431535, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=345, Percent_Identity=23.768115942029, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=396, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=136, Percent_Identity=31.6176470588235, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=265, Percent_Identity=23.3962264150943, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54260; Mature: 54260

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPALTTVETRPVDPRLTLGLVCLAIFIGAVDLTVISAALPKVMIDLRLSLDTELNRAS
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
WAVSGYLLAYTVSMTFMGRLSDLFGRRMVYLICLVVFLIGSAWVAAAPNLTMLIIGRVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
AFGAGAMVPVSMALVGDLFPPGQRAAALGLIGAVDTAGWMVGHLYGGVLMRIFDDWRLLF
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
WLNLPIGLVALALTWYALRQVPTPPRVGRFDWPGTLLLSASLVALNIGLAAGSELGATDF
EECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEECHHCCCCCCCCCC
YGERLGPPPYAGPLVIVSLLLLALFVWVERRSDDPLIGLELFTHRHTAMACVINVMVGFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LAIAITNVPLYINTRLLLYHPTDSDILRIAAWDAGWMLSALTLTMAAAALPGGLLTGRFG
HHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHH
ARLPAMLGLGLAIIGYGLMAFWGPDATYLRMGLELALTGIGLGLVIAPVADTVIAAAGED
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRGAASALVIALRLVGMTVGVAILTLWGVHRQDELRRAGADNPLAVTEPARFLMEIAARV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECHHHHHHHHHHHHH
IGETFLFGAAACLVGLLAALVMREVSGRRT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNPALTTVETRPVDPRLTLGLVCLAIFIGAVDLTVISAALPKVMIDLRLSLDTELNRAS
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
WAVSGYLLAYTVSMTFMGRLSDLFGRRMVYLICLVVFLIGSAWVAAAPNLTMLIIGRVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
AFGAGAMVPVSMALVGDLFPPGQRAAALGLIGAVDTAGWMVGHLYGGVLMRIFDDWRLLF
HHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
WLNLPIGLVALALTWYALRQVPTPPRVGRFDWPGTLLLSASLVALNIGLAAGSELGATDF
EECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEECHHCCCCCCCCCC
YGERLGPPPYAGPLVIVSLLLLALFVWVERRSDDPLIGLELFTHRHTAMACVINVMVGFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LAIAITNVPLYINTRLLLYHPTDSDILRIAAWDAGWMLSALTLTMAAAALPGGLLTGRFG
HHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHH
ARLPAMLGLGLAIIGYGLMAFWGPDATYLRMGLELALTGIGLGLVIAPVADTVIAAAGED
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRGAASALVIALRLVGMTVGVAILTLWGVHRQDELRRAGADNPLAVTEPARFLMEIAARV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECHHHHHHHHHHHHH
IGETFLFGAAACLVGLLAALVMREVSGRRT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]