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The map label for this gene is lipA

Identifier: 222526309

GI number: 222526309

Start: 3793758

End: 3794678

Strand: Direct

Name: lipA

Synonym: Chy400_3075

Alternate gene names: 222526309

Gene position: 3793758-3794678 (Clockwise)

Preceding gene: 222526308

Following gene: 222526310

Centisome position: 72.0

GC content: 56.68

Gene sequence:

>921_bases
ATGGCAGAACTTATTCCGCTAAATGAGGTAGGTGTCGCGCAACCGGCATCAGGTGCAGTCAATCGGCCACGCCGCCCGGA
GTGGTTGAAAGCGCGGGCACCGGGTGGGGTGAATTATCACGATGTGTTGCGCTTGATGCGCGAAAAGAATCTGCATACGG
TCTGCGAAGAAGCCCGCTGTCCAAATATCGGTGAGTGCTGGAATCATCGCACTGCCACCTTTCTCTTGCTCGGTGATATT
TGTACCCGTGGTTGTCGCTACTGCGCTATCGGTAAAGGGAAACCAAAGCCAATTGACGAGAACGAGCCGGAGCGGGTTGC
CGAGTCGGTTGCTCACCTGAAACTCAAGTTTGCCGTGCTCACGTCGGTCAACCGTGATGATGTACCCGACGGTGGTGCGC
ATATTTTTGCCCGTACTATCGAGCTTATCCGCCAGAAGGTGCCTGATTGCAAGGTCGAAGTGCTCATCCCCGATTTCGAT
GGTAATTGGGATGCACTGGCGACGGTGCTGGCCGCCGAACCCGATGTGCTGAACCACAATATCGAGACGGTACCGCGTCT
GTTCCGGCGGTTTCGACCACGCGCTAAATTCGAGCAGAGTATTGAATTGCTGGCCAGAGCACGGGCTGCTCGCCCCAAGC
TGGTTACCAAGAGTGGCATGATGGTCGGTGCCGGTGAGACCAACGAAGAGGTGTACGAGGTGATTGATCGTCTGCGCAGT
GTTGATGTGAATGTGCTGACGATTGGTCAGTATCTGGCACCCGATGCGAGCTATTGGCCGGTGCATCGCTATGTCACGCC
CGCCGAGTTTGCTGAGTTTCGCAGCTACGCGCTGGCCCGTGGTTTTACGCACGTTGAGAGCGGGCCGCTGGTGCGGTCGA
GTTACAATGCCCATCTGCACGTGGGCGCTGCCCAACATTAG

Upstream 100 bases:

>100_bases
GCTAACTTTCTAATAATATGCGGTACAATACGTATGCCAATCGAATCGTTATCGTTGATCGTCCGACCCGGTCGGCGATC
ACTGGAAGGCGAGAACGCCA

Downstream 100 bases:

>100_bases
ACGCAGGATTGCTCGCGCAGGGAGTGATAGAGTACCTGCGCGATTGCCTATCATTTCAATGCTGTTGTATCTTTTGGTAA
CGGCATCTGAGTGTTGCGTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAELIPLNEVGVAQPASGAVNRPRRPEWLKARAPGGVNYHDVLRLMREKNLHTVCEEARCPNIGECWNHRTATFLLLGDI
CTRGCRYCAIGKGKPKPIDENEPERVAESVAHLKLKFAVLTSVNRDDVPDGGAHIFARTIELIRQKVPDCKVEVLIPDFD
GNWDALATVLAAEPDVLNHNIETVPRLFRRFRPRAKFEQSIELLARARAARPKLVTKSGMMVGAGETNEEVYEVIDRLRS
VDVNVLTIGQYLAPDASYWPVHRYVTPAEFAEFRSYALARGFTHVESGPLVRSSYNAHLHVGAAQH

Sequences:

>Translated_306_residues
MAELIPLNEVGVAQPASGAVNRPRRPEWLKARAPGGVNYHDVLRLMREKNLHTVCEEARCPNIGECWNHRTATFLLLGDI
CTRGCRYCAIGKGKPKPIDENEPERVAESVAHLKLKFAVLTSVNRDDVPDGGAHIFARTIELIRQKVPDCKVEVLIPDFD
GNWDALATVLAAEPDVLNHNIETVPRLFRRFRPRAKFEQSIELLARARAARPKLVTKSGMMVGAGETNEEVYEVIDRLRS
VDVNVLTIGQYLAPDASYWPVHRYVTPAEFAEFRSYALARGFTHVESGPLVRSSYNAHLHVGAAQH
>Mature_305_residues
AELIPLNEVGVAQPASGAVNRPRRPEWLKARAPGGVNYHDVLRLMREKNLHTVCEEARCPNIGECWNHRTATFLLLGDIC
TRGCRYCAIGKGKPKPIDENEPERVAESVAHLKLKFAVLTSVNRDDVPDGGAHIFARTIELIRQKVPDCKVEVLIPDFDG
NWDALATVLAAEPDVLNHNIETVPRLFRRFRPRAKFEQSIELLARARAARPKLVTKSGMMVGAGETNEEVYEVIDRLRSV
DVNVLTIGQYLAPDASYWPVHRYVTPAEFAEFRSYALARGFTHVESGPLVRSSYNAHLHVGAAQH

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=45.583038869258, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=45.5645161290323, Blast_Score=234, Evalue=6e-62,
Organism=Escherichia coli, GI1786846, Length=299, Percent_Identity=49.1638795986622, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=42.6573426573427, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=44.8028673835125, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI221513272, Length=294, Percent_Identity=44.5578231292517, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHLAA (A9WEM1)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636429.1
- ProteinModelPortal:   A9WEM1
- GeneID:   5827310
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2839
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34201; Mature: 34069

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELIPLNEVGVAQPASGAVNRPRRPEWLKARAPGGVNYHDVLRLMREKNLHTVCEEARC
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCC
PNIGECWNHRTATFLLLGDICTRGCRYCAIGKGKPKPIDENEPERVAESVAHLKLKFAVL
CCHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TSVNRDDVPDGGAHIFARTIELIRQKVPDCKVEVLIPDFDGNWDALATVLAAEPDVLNHN
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHCCC
IETVPRLFRRFRPRAKFEQSIELLARARAARPKLVTKSGMMVGAGETNEEVYEVIDRLRS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHH
VDVNVLTIGQYLAPDASYWPVHRYVTPAEFAEFRSYALARGFTHVESGPLVRSSYNAHLH
CCCEEEEECHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHCCCCCCEECCCCCEEE
VGAAQH
ECCCCC
>Mature Secondary Structure 
AELIPLNEVGVAQPASGAVNRPRRPEWLKARAPGGVNYHDVLRLMREKNLHTVCEEARC
CCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCC
PNIGECWNHRTATFLLLGDICTRGCRYCAIGKGKPKPIDENEPERVAESVAHLKLKFAVL
CCHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TSVNRDDVPDGGAHIFARTIELIRQKVPDCKVEVLIPDFDGNWDALATVLAAEPDVLNHN
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHCCC
IETVPRLFRRFRPRAKFEQSIELLARARAARPKLVTKSGMMVGAGETNEEVYEVIDRLRS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHHHHHHH
VDVNVLTIGQYLAPDASYWPVHRYVTPAEFAEFRSYALARGFTHVESGPLVRSSYNAHLH
CCCEEEEECHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHCCCCCCEECCCCCEEE
VGAAQH
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA