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The map label for this gene is trmE

Identifier: 222526288

GI number: 222526288

Start: 3767140

End: 3768498

Strand: Direct

Name: trmE

Synonym: Chy400_3054

Alternate gene names: 222526288

Gene position: 3767140-3768498 (Clockwise)

Preceding gene: 222526286

Following gene: 222526289

Centisome position: 71.5

GC content: 60.56

Gene sequence:

>1359_bases
ATGAATGACACAATTGCTGCAATTGCCACACCACCCGGAGAAGGTGGCATTGGTATTATACGTCTGAGTGGGCCTGACGC
TCAATCGATTGCGTTGCGCATTTTTCGCCCCGTGCGGCCAGGACGGTTGCGCAGTCATCGGGTGCGCTATGGGCACGTGA
TCGGGCCTGATGGCGAAGTGATCGACGAAGCATTGCTAACGCTGATGGCCGCTCCGCATAGCTTTACCCGTGAGGATGTC
GTGGAAATCTCTTGCCATGGTGGGGCGCTTCCAGTCCAGTTGACGCTCGAAGCGGCCCTGGCTGCCGGTGCCCGCCTGGC
GAATCCCGGTGAATTTACCTTACGGGCGTTCCTCAATGGTCGGATCGATCTGAGTCAGGCCGAAGCCACCCTCGACGTAA
TTCGGGCGCAGACGAGTGCCGGGCTGGCGATTGCCCAGGCGCAGTTGGGGGGCTGGCTGGCACGCGAAGTACGGGCTGCC
CGCACAGCGATCCTTGAACCGCTGGCCTATATCACTGCGCTGATCGATTTTCCTGAAGAGGGGATTGAACCGCAGACGGT
GGCCGGGCCAATTGAACAGGCACTGGCCACTGTCGAGCGCTTACTGGCCGGGGCCGATCAGGGCATGGTCTTGCGCAACG
GCGCGCGCGTCGTGCTGGTTGGCCGCCCAAATGTTGGCAAGTCAAGCCTGCTTAATGCCCTGCTCCGGGTTGAACGCGCT
ATAGTTACCCCTATCCCCGGTACAACGCGCGACACCCTGGAAGAGATGGCAAATCTGGCGGGTGTACCGGTGGTGTTAAT
CGACACCGCCGGTATGCGCACCAGCACCGATCCGGTCGAACAGATTGGGGTGGAGCGAGCAGCAGCGGCGCTGGCCGGTG
CCGACCTGGCCCTACTTGTCTTCGACTCATCACAACCGTTTACCCCCGAAGATGAAGCGATGCTGGTTGCCACTGCCGAC
CGACCGACCATCATTGTCTGGAACAAGTGCGATGATCCGGATGTACCGCCCCCACCCGCTCCGCCGCATCCGAAAGCGAT
GGCTGTGGTTGCCTGTTCGGCTCGCTATGGCCATGGGATCGATACGCTGGCTAAAACAATTGCCACGACCCTGCTCGGTG
GTACATTACCGGCTGTTGGCGCAACGCATCTGGTCAGCAATCCGCGTCACCGGGCTGCCCTGCGGCGAGCCGCCGAGTTT
CTGCGTGCAGCTCAAGAAACCCTGGCTGCCGGTGCGGCAACCGATCTCCTGGCAGCCGATTTAACCGGTGCTGCCAATGC
GTTGGGTGAGATTACCGGCGAAACCGTGGGTGAAGATTTGCTCGACATGATCTTCAGCCGTTTTTGCATCGGCAAATAG

Upstream 100 bases:

>100_bases
GGTTGCTCCAGCTCAACCCTCGCAGACCACTGAGATGGGCAACTGCGTTACCGAGTGTAATCAGCCGTAAAAAGGTATTA
AGTAAACGCACAGAACCTCG

Downstream 100 bases:

>100_bases
CCCCTGTGTTACAATGACAGCGGGCCGTTATACCGCGAATAAGGAGGAACGCGCATGAACAAGAAGACCATCCGTGATGT
GGATTGGGCCGGAAAACGTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNDTIAAIATPPGEGGIGIIRLSGPDAQSIALRIFRPVRPGRLRSHRVRYGHVIGPDGEVIDEALLTLMAAPHSFTREDV
VEISCHGGALPVQLTLEAALAAGARLANPGEFTLRAFLNGRIDLSQAEATLDVIRAQTSAGLAIAQAQLGGWLAREVRAA
RTAILEPLAYITALIDFPEEGIEPQTVAGPIEQALATVERLLAGADQGMVLRNGARVVLVGRPNVGKSSLLNALLRVERA
IVTPIPGTTRDTLEEMANLAGVPVVLIDTAGMRTSTDPVEQIGVERAAAALAGADLALLVFDSSQPFTPEDEAMLVATAD
RPTIIVWNKCDDPDVPPPPAPPHPKAMAVVACSARYGHGIDTLAKTIATTLLGGTLPAVGATHLVSNPRHRAALRRAAEF
LRAAQETLAAGAATDLLAADLTGAANALGEITGETVGEDLLDMIFSRFCIGK

Sequences:

>Translated_452_residues
MNDTIAAIATPPGEGGIGIIRLSGPDAQSIALRIFRPVRPGRLRSHRVRYGHVIGPDGEVIDEALLTLMAAPHSFTREDV
VEISCHGGALPVQLTLEAALAAGARLANPGEFTLRAFLNGRIDLSQAEATLDVIRAQTSAGLAIAQAQLGGWLAREVRAA
RTAILEPLAYITALIDFPEEGIEPQTVAGPIEQALATVERLLAGADQGMVLRNGARVVLVGRPNVGKSSLLNALLRVERA
IVTPIPGTTRDTLEEMANLAGVPVVLIDTAGMRTSTDPVEQIGVERAAAALAGADLALLVFDSSQPFTPEDEAMLVATAD
RPTIIVWNKCDDPDVPPPPAPPHPKAMAVVACSARYGHGIDTLAKTIATTLLGGTLPAVGATHLVSNPRHRAALRRAAEF
LRAAQETLAAGAATDLLAADLTGAANALGEITGETVGEDLLDMIFSRFCIGK
>Mature_452_residues
MNDTIAAIATPPGEGGIGIIRLSGPDAQSIALRIFRPVRPGRLRSHRVRYGHVIGPDGEVIDEALLTLMAAPHSFTREDV
VEISCHGGALPVQLTLEAALAAGARLANPGEFTLRAFLNGRIDLSQAEATLDVIRAQTSAGLAIAQAQLGGWLAREVRAA
RTAILEPLAYITALIDFPEEGIEPQTVAGPIEQALATVERLLAGADQGMVLRNGARVVLVGRPNVGKSSLLNALLRVERA
IVTPIPGTTRDTLEEMANLAGVPVVLIDTAGMRTSTDPVEQIGVERAAAALAGADLALLVFDSSQPFTPEDEAMLVATAD
RPTIIVWNKCDDPDVPPPPAPPHPKAMAVVACSARYGHGIDTLAKTIATTLLGGTLPAVGATHLVSNPRHRAALRRAAEF
LRAAQETLAAGAATDLLAADLTGAANALGEITGETVGEDLLDMIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=36.5957446808511, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=35.9477124183007, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=34.0681362725451, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=39.524838012959, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI87082120, Length=121, Percent_Identity=39.6694214876033, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=31.7494600431965, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=494, Percent_Identity=30.3643724696356, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=33.5416666666667, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLAA (A9WKE3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636410.1
- ProteinModelPortal:   A9WKE3
- SMR:   A9WKE3
- GeneID:   5827291
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2820
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47167; Mature: 47167

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 82-82 BINDING 121-121 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTIAAIATPPGEGGIGIIRLSGPDAQSIALRIFRPVRPGRLRSHRVRYGHVIGPDGEV
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECEEECCCHHH
IDEALLTLMAAPHSFTREDVVEISCHGGALPVQLTLEAALAAGARLANPGEFTLRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEECCC
RIDLSQAEATLDVIRAQTSAGLAIAQAQLGGWLAREVRAARTAILEPLAYITALIDFPEE
CEEHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIEPQTVAGPIEQALATVERLLAGADQGMVLRNGARVVLVGRPNVGKSSLLNALLRVERA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
IVTPIPGTTRDTLEEMANLAGVPVVLIDTAGMRTSTDPVEQIGVERAAAALAGADLALLV
HCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
FDSSQPFTPEDEAMLVATADRPTIIVWNKCDDPDVPPPPAPPHPKAMAVVACSARYGHGI
EECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
DTLAKTIATTLLGGTLPAVGATHLVSNPRHRAALRRAAEFLRAAQETLAAGAATDLLAAD
HHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LTGAANALGEITGETVGEDLLDMIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDTIAAIATPPGEGGIGIIRLSGPDAQSIALRIFRPVRPGRLRSHRVRYGHVIGPDGEV
CCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEECEEECCCHHH
IDEALLTLMAAPHSFTREDVVEISCHGGALPVQLTLEAALAAGARLANPGEFTLRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEECCC
RIDLSQAEATLDVIRAQTSAGLAIAQAQLGGWLAREVRAARTAILEPLAYITALIDFPEE
CEEHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIEPQTVAGPIEQALATVERLLAGADQGMVLRNGARVVLVGRPNVGKSSLLNALLRVERA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
IVTPIPGTTRDTLEEMANLAGVPVVLIDTAGMRTSTDPVEQIGVERAAAALAGADLALLV
HCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEE
FDSSQPFTPEDEAMLVATADRPTIIVWNKCDDPDVPPPPAPPHPKAMAVVACSARYGHGI
EECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
DTLAKTIATTLLGGTLPAVGATHLVSNPRHRAALRRAAEFLRAAQETLAAGAATDLLAAD
HHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LTGAANALGEITGETVGEDLLDMIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA