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The map label for this gene is pgk

Identifier: 222526289

GI number: 222526289

Start: 3768553

End: 3769755

Strand: Direct

Name: pgk

Synonym: Chy400_3055

Alternate gene names: 222526289

Gene position: 3768553-3769755 (Clockwise)

Preceding gene: 222526288

Following gene: 222526292

Centisome position: 71.52

GC content: 56.86

Gene sequence:

>1203_bases
ATGAACAAGAAGACCATCCGTGATGTGGATTGGGCCGGAAAACGTGCGCTGGTACGAGTTGATTTCAACGTGCCACTCGA
TGATCAGGGTCAGATTACCGATGATACCCGGATTCGGGCGGCATTACCGACCATTCGCTATCTGCTGGAACACGGTGCCA
GTGTGGTCTTGATGTCGCACCTGGGGCGTCCGAAGGGTAAACCCAATCCGAAGTACAGTTTACGTCCGGTTGTTGAGCGT
TTGTTTGAATTGCTGCCCGAAGCGAAAGAGGTCAAGAAGACCGAGGCGATCACCGGGCCGGCGGCTGAAGCGGCTGTGGC
GATGCTGAAACCAGGGCAGGTCCTGGTACTCGAAAACACCCGCTTCGATCCCCGTGAAGAGCCAAACGATCCAGCGATGG
CCGCTGAACTGGCGAAATTGGGTGATGTTTTCGTTAACGATGCCTTCGGCACCGCTCACCGGGCCAATGCCAGCACTGAG
GGTGTAGCGCACTACCTGCCGGCAGTTGCCGGTTTTCTTATGGAAAAGGAACTGACGTACATCGGTGGCGCCCTGAATAA
TCCGCAGCGTCCATTCGTAACCGTCATCGGTGGTGCGAAAATCAGTGACAAGATCGGCGTGATCGAAAATCTGCTGGGTA
AGGTCGATGCACTGCTCATTGGTGGTGGTATGGCCAACACCTTTCTGCTTGCCAAAGGGCTGAATGTTGGTGATTCGCTG
GTCGAACCGGACAGTGTACCGGTGGCCCAGCAGTTGATGGCCCGTGCTGAAGAGCGGGGTGCCCGGCTGCTTTTGCCGGT
TGACGTGGTGATTGCCGATGCGTTTAGTGCCGATGCCCAGCGCCAGGTTGTTGATGTAAGTGACATTCCTGCCGGCTGGC
GTGTGCTCGATATTGGCCCCAAGACCATCGAACGATACAGTGCCGAGATTCGGGCTGCCCGCACGGTTATCTGGAATGGG
CCAATGGGTGTCTTTGAACTAGAGCCGTTTGCCGTCGGTACACGCGCTATCGCCCAGGCAATGGCCGAAGCGGCTGCCAA
TGGCGCAATCACAATTGTTGGTGGTGGCGATAGTGTGGCCGCAGTGGAACAGGCCGGCCTGGCCGATAAGATGTCGCATG
TGAGTACCGGCGGTGGTGCCTCACTTGAGTTACTTGAGGGACGAGTCTTACCCGGTGTGGCTGCGTTGCAAGATGCAGAG
TAG

Upstream 100 bases:

>100_bases
AGATTTGCTCGACATGATCTTCAGCCGTTTTTGCATCGGCAAATAGCCCCTGTGTTACAATGACAGCGGGCCGTTATACC
GCGAATAAGGAGGAACGCGC

Downstream 100 bases:

>100_bases
TCTGGGTTAAGCAGGATCGTCTTCGGGTGACCTATGGACGTAGTTTGATCCATCCTACGCCCGCCGCTTTTTTGGTACGG
GTTAAGCAGGATCGTCTTCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNKKTIRDVDWAGKRALVRVDFNVPLDDQGQITDDTRIRAALPTIRYLLEHGASVVLMSHLGRPKGKPNPKYSLRPVVER
LFELLPEAKEVKKTEAITGPAAEAAVAMLKPGQVLVLENTRFDPREEPNDPAMAAELAKLGDVFVNDAFGTAHRANASTE
GVAHYLPAVAGFLMEKELTYIGGALNNPQRPFVTVIGGAKISDKIGVIENLLGKVDALLIGGGMANTFLLAKGLNVGDSL
VEPDSVPVAQQLMARAEERGARLLLPVDVVIADAFSADAQRQVVDVSDIPAGWRVLDIGPKTIERYSAEIRAARTVIWNG
PMGVFELEPFAVGTRAIAQAMAEAAANGAITIVGGGDSVAAVEQAGLADKMSHVSTGGGASLELLEGRVLPGVAALQDAE

Sequences:

>Translated_400_residues
MNKKTIRDVDWAGKRALVRVDFNVPLDDQGQITDDTRIRAALPTIRYLLEHGASVVLMSHLGRPKGKPNPKYSLRPVVER
LFELLPEAKEVKKTEAITGPAAEAAVAMLKPGQVLVLENTRFDPREEPNDPAMAAELAKLGDVFVNDAFGTAHRANASTE
GVAHYLPAVAGFLMEKELTYIGGALNNPQRPFVTVIGGAKISDKIGVIENLLGKVDALLIGGGMANTFLLAKGLNVGDSL
VEPDSVPVAQQLMARAEERGARLLLPVDVVIADAFSADAQRQVVDVSDIPAGWRVLDIGPKTIERYSAEIRAARTVIWNG
PMGVFELEPFAVGTRAIAQAMAEAAANGAITIVGGGDSVAAVEQAGLADKMSHVSTGGGASLELLEGRVLPGVAALQDAE
>Mature_400_residues
MNKKTIRDVDWAGKRALVRVDFNVPLDDQGQITDDTRIRAALPTIRYLLEHGASVVLMSHLGRPKGKPNPKYSLRPVVER
LFELLPEAKEVKKTEAITGPAAEAAVAMLKPGQVLVLENTRFDPREEPNDPAMAAELAKLGDVFVNDAFGTAHRANASTE
GVAHYLPAVAGFLMEKELTYIGGALNNPQRPFVTVIGGAKISDKIGVIENLLGKVDALLIGGGMANTFLLAKGLNVGDSL
VEPDSVPVAQQLMARAEERGARLLLPVDVVIADAFSADAQRQVVDVSDIPAGWRVLDIGPKTIERYSAEIRAARTVIWNG
PMGVFELEPFAVGTRAIAQAMAEAAANGAITIVGGGDSVAAVEQAGLADKMSHVSTGGGASLELLEGRVLPGVAALQDAE

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=417, Percent_Identity=48.9208633093525, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=47.5961538461538, Blast_Score=358, Evalue=5e-99,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=43.25, Blast_Score=307, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=49.0430622009569, Blast_Score=379, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=48.4412470023981, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=46.7625899280576, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=44.2583732057416, Blast_Score=325, Evalue=3e-89,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CHLAA (A9WKE4)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636411.1
- ProteinModelPortal:   A9WKE4
- SMR:   A9WKE4
- GeneID:   5827292
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2821
- HOGENOM:   HBG453500
- OMA:   LSYFSKA
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42310; Mature: 42310

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 121-121 BINDING 154-154 BINDING 204-204 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTIRDVDWAGKRALVRVDFNVPLDDQGQITDDTRIRAALPTIRYLLEHGASVVLMSH
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHH
LGRPKGKPNPKYSLRPVVERLFELLPEAKEVKKTEAITGPAAEAAVAMLKPGQVLVLENT
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHEECCCEEEEEECC
RFDPREEPNDPAMAAELAKLGDVFVNDAFGTAHRANASTEGVAHYLPAVAGFLMEKELTY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGGALNNPQRPFVTVIGGAKISDKIGVIENLLGKVDALLIGGGMANTFLLAKGLNVGDSL
HCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHEECCCCCCCCC
VEPDSVPVAQQLMARAEERGARLLLPVDVVIADAFSADAQRQVVDVSDIPAGWRVLDIGP
CCCCCCCHHHHHHHHHHHCCCEEEEEHHEEEEHHHCCCCHHEEEECCCCCCCCEEEECCH
KTIERYSAEIRAARTVIWNGPMGVFELEPFAVGTRAIAQAMAEAAANGAITIVGGGDSVA
HHHHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
AVEQAGLADKMSHVSTGGGASLELLEGRVLPGVAALQDAE
HHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHCCCC
>Mature Secondary Structure
MNKKTIRDVDWAGKRALVRVDFNVPLDDQGQITDDTRIRAALPTIRYLLEHGASVVLMSH
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHH
LGRPKGKPNPKYSLRPVVERLFELLPEAKEVKKTEAITGPAAEAAVAMLKPGQVLVLENT
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHEECCCEEEEEECC
RFDPREEPNDPAMAAELAKLGDVFVNDAFGTAHRANASTEGVAHYLPAVAGFLMEKELTY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IGGALNNPQRPFVTVIGGAKISDKIGVIENLLGKVDALLIGGGMANTFLLAKGLNVGDSL
HCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHEECCCCCCCCC
VEPDSVPVAQQLMARAEERGARLLLPVDVVIADAFSADAQRQVVDVSDIPAGWRVLDIGP
CCCCCCCHHHHHHHHHHHCCCEEEEEHHEEEEHHHCCCCHHEEEECCCCCCCCEEEECCH
KTIERYSAEIRAARTVIWNGPMGVFELEPFAVGTRAIAQAMAEAAANGAITIVGGGDSVA
HHHHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
AVEQAGLADKMSHVSTGGGASLELLEGRVLPGVAALQDAE
HHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA