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The map label for this gene is gca [H]

Identifier: 222526146

GI number: 222526146

Start: 3587339

End: 3588313

Strand: Direct

Name: gca [H]

Synonym: Chy400_2903

Alternate gene names: 222526146

Gene position: 3587339-3588313 (Clockwise)

Preceding gene: 222526141

Following gene: 222526147

Centisome position: 68.08

GC content: 57.85

Gene sequence:

>975_bases
ATGCGGGCACTCATTACTGGCATTAACGGTTTTGTTGGCGGCCACCTCGCCGAATATCTCCTGGCCGATGGACGCTGGGA
TGTTTGGGGATTATCTCGCTCTCCTTCGCTCGTACTTCCCGAACTGATCGGGAACGTGCAGATCGTCCAGGCCGATCTGG
CCGATGCTGAGGCCACTACGCGCGCTCTGGTGCAGGTGCGACCGAACGTCATTTTTCACCTTGCCGGCCAGCCATTCGTG
CCAGAATCGTTTCGCGACCCGGCGGGAACGCTGGCCGCCAATACGTTGGGCGCCTTGCACATCTTTCTGACGTTGATTGA
ATACCGCATGACCACCCGCGTGATAGTGATTGGCACGAATGAGGAATACGGAAAGATTGACCCGGAGGATTTACCGATTG
ACGAAGACACCCCCCTGCGTCCGACCAGTCCGTATGGGGTGAGCAAGGCCGCCCAAAGTTTGCTGGCGTTGCAATACCAC
TACAGCCACGGTCTGGATGTCGTGCGTGTACGGCCCTTCACCCATATCGGGCCGCGCCAGAATGAACGATTTGTGACTGC
CGCCTTTGCCCGTCAGATTGCACGGATCGAATTAGGCTTGCAACCGCCAGTTGTGCAGGTTGGTAATCTGGCTGCGCAAC
GCGACTTCACCGATGTCCGTGATGTTGTCGCGGCGTATGCGTTACTGGCCGAACACGGCGAGAGTGGCGAGGTGTATAAT
GTTGGTTCGGGTCGGGCCGTGATGATACGCGAGCTACTTGATATGCTGTTAGCCGAATGTACTGTACCGGTTGAAGTGCG
GCTCAATCCCGAATTGATGCGCCCGATTGATATACCGCTGGTCGTCTGTGATGCGAGTCGGCTCCGTGCCCGCACCGGCT
GGCAACCGCGCTATACGCTGGCAGAGACGCTGCACGATATCCTGAATTACTGGCGCGCACGAGTCCGTGCAGAAGTACAA
GCGACCACCGGCTGA

Upstream 100 bases:

>100_bases
GTGGTACAATGTTACAAATATTGTAACACGGTGCACAATCACTGTCAATAAATTGCACAATCAAACAAGACACTTCTGAA
CACAAGGTATCAATAATACC

Downstream 100 bases:

>100_bases
GAAAATCGTCACCCAAACCCCGGTTTGCGGTAAACTTTTTGTGGTCTGATGGCGTTATACTACGCCTATCTATCAGGAGC
GTTGTGTACATTTAGTAAGT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRALITGINGFVGGHLAEYLLADGRWDVWGLSRSPSLVLPELIGNVQIVQADLADAEATTRALVQVRPNVIFHLAGQPFV
PESFRDPAGTLAANTLGALHIFLTLIEYRMTTRVIVIGTNEEYGKIDPEDLPIDEDTPLRPTSPYGVSKAAQSLLALQYH
YSHGLDVVRVRPFTHIGPRQNERFVTAAFARQIARIELGLQPPVVQVGNLAAQRDFTDVRDVVAAYALLAEHGESGEVYN
VGSGRAVMIRELLDMLLAECTVPVEVRLNPELMRPIDIPLVVCDASRLRARTGWQPRYTLAETLHDILNYWRARVRAEVQ
ATTG

Sequences:

>Translated_324_residues
MRALITGINGFVGGHLAEYLLADGRWDVWGLSRSPSLVLPELIGNVQIVQADLADAEATTRALVQVRPNVIFHLAGQPFV
PESFRDPAGTLAANTLGALHIFLTLIEYRMTTRVIVIGTNEEYGKIDPEDLPIDEDTPLRPTSPYGVSKAAQSLLALQYH
YSHGLDVVRVRPFTHIGPRQNERFVTAAFARQIARIELGLQPPVVQVGNLAAQRDFTDVRDVVAAYALLAEHGESGEVYN
VGSGRAVMIRELLDMLLAECTVPVEVRLNPELMRPIDIPLVVCDASRLRARTGWQPRYTLAETLHDILNYWRARVRAEVQ
ATTG
>Mature_324_residues
MRALITGINGFVGGHLAEYLLADGRWDVWGLSRSPSLVLPELIGNVQIVQADLADAEATTRALVQVRPNVIFHLAGQPFV
PESFRDPAGTLAANTLGALHIFLTLIEYRMTTRVIVIGTNEEYGKIDPEDLPIDEDTPLRPTSPYGVSKAAQSLLALQYH
YSHGLDVVRVRPFTHIGPRQNERFVTAAFARQIARIELGLQPPVVQVGNLAAQRDFTDVRDVVAAYALLAEHGESGEVYN
VGSGRAVMIRELLDMLLAECTVPVEVRLNPELMRPIDIPLVVCDASRLRARTGWQPRYTLAETLHDILNYWRARVRAEVQ
ATTG

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=27.4336283185841, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=25, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=25.5747126436782, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=23.342939481268, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=24.7863247863248, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788589, Length=272, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=26.2536873156342, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI133901786, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI133901788, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI133901790, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17539424, Length=339, Percent_Identity=27.1386430678466, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=27.1386430678466, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=26.4437689969605, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=24.207492795389, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=23.8505747126437, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=23.4939759036145, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24158427, Length=339, Percent_Identity=27.7286135693215, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35815; Mature: 35815

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALITGINGFVGGHLAEYLLADGRWDVWGLSRSPSLVLPELIGNVQIVQADLADAEATT
CCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHCCEEEEEECCCCHHHHH
RALVQVRPNVIFHLAGQPFVPESFRDPAGTLAANTLGALHIFLTLIEYRMTTRVIVIGTN
HHHHEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
EEYGKIDPEDLPIDEDTPLRPTSPYGVSKAAQSLLALQYHYSHGLDVVRVRPFTHIGPRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
NERFVTAAFARQIARIELGLQPPVVQVGNLAAQRDFTDVRDVVAAYALLAEHGESGEVYN
CCCEEHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEE
VGSGRAVMIRELLDMLLAECTVPVEVRLNPELMRPIDIPLVVCDASRLRARTGWQPRYTL
CCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCEEEECCHHHHHHCCCCCCHHH
AETLHDILNYWRARVRAEVQATTG
HHHHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MRALITGINGFVGGHLAEYLLADGRWDVWGLSRSPSLVLPELIGNVQIVQADLADAEATT
CCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHCCEEEEEECCCCHHHHH
RALVQVRPNVIFHLAGQPFVPESFRDPAGTLAANTLGALHIFLTLIEYRMTTRVIVIGTN
HHHHEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
EEYGKIDPEDLPIDEDTPLRPTSPYGVSKAAQSLLALQYHYSHGLDVVRVRPFTHIGPRQ
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
NERFVTAAFARQIARIELGLQPPVVQVGNLAAQRDFTDVRDVVAAYALLAEHGESGEVYN
CCCEEHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEE
VGSGRAVMIRELLDMLLAECTVPVEVRLNPELMRPIDIPLVVCDASRLRARTGWQPRYTL
CCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCEEEECCHHHHHHCCCCCCHHH
AETLHDILNYWRARVRAEVQATTG
HHHHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]