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The map label for this gene is rlmI [H]

Identifier: 222525966

GI number: 222525966

Start: 3358411

End: 3359595

Strand: Reverse

Name: rlmI [H]

Synonym: Chy400_2722

Alternate gene names: 222525966

Gene position: 3359595-3358411 (Counterclockwise)

Preceding gene: 222525967

Following gene: 222525965

Centisome position: 63.76

GC content: 56.79

Gene sequence:

>1185_bases
ATGTCAACCATTACCGAACTGACCATAGAACCATCACTACGCTCACGTCTCCAGCAAGGTCATCCGTGGGTCTACCGCAA
TCACCTGATCGGTGGCGAACGATTGCGCAGCGGGCAATGGGTACGCGCCCGCTGCGGTGGTTTGACCGTTTACGGTTTAT
ACGACGAGCAGAGTCCTATTGGCTTGCGAATCTATAGCCGGAATGGTATGCCAGACCAATCCTGGTTTCGCGAACGAGTC
TGGGAAGCATGGGAGCTACGCGCACCATTACGGGCAGATGGGCAGACAACAGCCTATCGCTGGATCTACGGCGAGGGGGA
TAATCTGCCCGGACTGGTGGTTGATCGGTACGGCGATTATGCCGTCATCCAGACCTACGCCGACAGTGTGCAAACGGTGA
TACCGGCCATTACTTCTGCTCTGCGTGCTATCGACCCTGACCTGCGTGGTGTGATCCAGCGACCACGGCAGACCGATGAT
AAGGACGAGGGTGAACCGGTGTTGTTGTGGGGCGAATGGCCGCCCCGCGACCTGGTTGTACAGGAGCATGGTCTCTTCTT
CCAGGTTGATATTCTCTACGGCCAGAAGACCGGCCTGTTTCTCGACCAGCGTGATAACCGGCGCACATTAGAAGGTCTGG
TCGCAGGCGCCAGGGTTCTCAACTGTTTTGCCTACACTGGCGGCTTCTCGCTCTACGCACTCCGCGGTCATGCAGCCGAA
GTTGTGAGCTGCGACATCGGTCGCGGGCTAGCAGAAGCGACTGCCACAAACATAGCGTTGAACCGGTTACCCGCCGAACG
TCACCGTTTCATAACTGAAGACTGCTTCGCTGTACTTGATCGCTTTGCCAGACAAAACCAAACCTTTGACGTTGTCATCC
TCGACCCACCGAGTTTTGCCCGTACCCGCACCAGTCAGCATGCCGCAATGCGTGCCTACGTGCGCCTCAACATGCTTGGA
TTGCGTTGCGTGACTCCCGGTGGATTACTGGTCAGTGCCAGTTGTACGGCCCAAATCGGGCCTGAACAGTTCCGCTCGCT
ACTTGGGGAAGCAGCGGCTCAGGCGCATCGTCGCCTTCAGATCATTCACGAAGCCGGCCATCCATTGGATCACCCGGTAC
CGGCAGGTTTTCCTGAAGGACGTTATCTGAAATACATCATTGCCAGGGCACAAACGCTGGTGTAG

Upstream 100 bases:

>100_bases
CCATCAAACCGCGCACGCAGCCGGCTGCATTTCCGGCTGATGGTCAGGTACCGAGTGGTGCTACCATCTAAATGAAAAAA
ACCGGCAATTAGTTAATAAC

Downstream 100 bases:

>100_bases
TGCCAGGGTATGCGCAGGCAACCAACACCACTTCACGACAGTTCTGAGACGGGCAGAAACAGGCAAGTGGATACGAGCGA
TCTGGATGTATCAGCCAGGG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSTITELTIEPSLRSRLQQGHPWVYRNHLIGGERLRSGQWVRARCGGLTVYGLYDEQSPIGLRIYSRNGMPDQSWFRERV
WEAWELRAPLRADGQTTAYRWIYGEGDNLPGLVVDRYGDYAVIQTYADSVQTVIPAITSALRAIDPDLRGVIQRPRQTDD
KDEGEPVLLWGEWPPRDLVVQEHGLFFQVDILYGQKTGLFLDQRDNRRTLEGLVAGARVLNCFAYTGGFSLYALRGHAAE
VVSCDIGRGLAEATATNIALNRLPAERHRFITEDCFAVLDRFARQNQTFDVVILDPPSFARTRTSQHAAMRAYVRLNMLG
LRCVTPGGLLVSASCTAQIGPEQFRSLLGEAAAQAHRRLQIIHEAGHPLDHPVPAGFPEGRYLKYIIARAQTLV

Sequences:

>Translated_394_residues
MSTITELTIEPSLRSRLQQGHPWVYRNHLIGGERLRSGQWVRARCGGLTVYGLYDEQSPIGLRIYSRNGMPDQSWFRERV
WEAWELRAPLRADGQTTAYRWIYGEGDNLPGLVVDRYGDYAVIQTYADSVQTVIPAITSALRAIDPDLRGVIQRPRQTDD
KDEGEPVLLWGEWPPRDLVVQEHGLFFQVDILYGQKTGLFLDQRDNRRTLEGLVAGARVLNCFAYTGGFSLYALRGHAAE
VVSCDIGRGLAEATATNIALNRLPAERHRFITEDCFAVLDRFARQNQTFDVVILDPPSFARTRTSQHAAMRAYVRLNMLG
LRCVTPGGLLVSASCTAQIGPEQFRSLLGEAAAQAHRRLQIIHEAGHPLDHPVPAGFPEGRYLKYIIARAQTLV
>Mature_393_residues
STITELTIEPSLRSRLQQGHPWVYRNHLIGGERLRSGQWVRARCGGLTVYGLYDEQSPIGLRIYSRNGMPDQSWFRERVW
EAWELRAPLRADGQTTAYRWIYGEGDNLPGLVVDRYGDYAVIQTYADSVQTVIPAITSALRAIDPDLRGVIQRPRQTDDK
DEGEPVLLWGEWPPRDLVVQEHGLFFQVDILYGQKTGLFLDQRDNRRTLEGLVAGARVLNCFAYTGGFSLYALRGHAAEV
VSCDIGRGLAEATATNIALNRLPAERHRFITEDCFAVLDRFARQNQTFDVVILDPPSFARTRTSQHAAMRAYVRLNMLGL
RCVTPGGLLVSASCTAQIGPEQFRSLLGEAAAQAHRRLQIIHEAGHPLDHPVPAGFPEGRYLKYIIARAQTLV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=377, Percent_Identity=33.42175066313, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1787181, Length=209, Percent_Identity=32.0574162679426, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44252; Mature: 44121

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTITELTIEPSLRSRLQQGHPWVYRNHLIGGERLRSGQWVRARCGGLTVYGLYDEQSPI
CCCCEEEEECHHHHHHHHCCCCEEEECCCCCCHHHCCCCEEEEECCCEEEEEEECCCCCC
GLRIYSRNGMPDQSWFRERVWEAWELRAPLRADGQTTAYRWIYGEGDNLPGLVVDRYGDY
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCE
AVIQTYADSVQTVIPAITSALRAIDPDLRGVIQRPRQTDDKDEGEPVLLWGEWPPRDLVV
EEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEE
QEHGLFFQVDILYGQKTGLFLDQRDNRRTLEGLVAGARVLNCFAYTGGFSLYALRGHAAE
ECCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
VVSCDIGRGLAEATATNIALNRLPAERHRFITEDCFAVLDRFARQNQTFDVVILDPPSFA
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
RTRTSQHAAMRAYVRLNMLGLRCVTPGGLLVSASCTAQIGPEQFRSLLGEAAAQAHRRLQ
HHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
IIHEAGHPLDHPVPAGFPEGRYLKYIIARAQTLV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STITELTIEPSLRSRLQQGHPWVYRNHLIGGERLRSGQWVRARCGGLTVYGLYDEQSPI
CCCEEEEECHHHHHHHHCCCCEEEECCCCCCHHHCCCCEEEEECCCEEEEEEECCCCCC
GLRIYSRNGMPDQSWFRERVWEAWELRAPLRADGQTTAYRWIYGEGDNLPGLVVDRYGDY
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCE
AVIQTYADSVQTVIPAITSALRAIDPDLRGVIQRPRQTDDKDEGEPVLLWGEWPPRDLVV
EEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEE
QEHGLFFQVDILYGQKTGLFLDQRDNRRTLEGLVAGARVLNCFAYTGGFSLYALRGHAAE
ECCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCE
VVSCDIGRGLAEATATNIALNRLPAERHRFITEDCFAVLDRFARQNQTFDVVILDPPSFA
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
RTRTSQHAAMRAYVRLNMLGLRCVTPGGLLVSASCTAQIGPEQFRSLLGEAAAQAHRRLQ
HHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
IIHEAGHPLDHPVPAGFPEGRYLKYIIARAQTLV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA