The gene/protein map for NC_012032 is currently unavailable.
Definition Chloroflexus sp. Y-400-fl chromosome, complete genome.
Accession NC_012032
Length 5,268,950

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The map label for this gene is tuf1

Identifier: 222525801

GI number: 222525801

Start: 3205961

End: 3207166

Strand: Direct

Name: tuf1

Synonym: Chy400_2554

Alternate gene names: 222525801

Gene position: 3205961-3207166 (Clockwise)

Preceding gene: 222525800

Following gene: 222525802

Centisome position: 60.85

GC content: 61.36

Gene sequence:

>1206_bases
ATGGCCAAGCAGAAATTCGAGCGGACGAAACCGCACATTAACGTCGGCACCATCGGTCACGTTGACCACGGCAAGACGAC
TCTGACCGCTGCGATTACCAAGGTGTTGTCGCTCAAGGGTGCAGCTCAGTTTATGGCGTATGACCAGATCGACAACGCCC
CCGAAGAGCGCGCCCGTGGCATTACGATTGCCATTCGCCACGTCGAGTATCAGACCGACAAGCGCCACTATGCGCACGTC
GACTGCCCCGGTCACGCCGACTATATCAAGAATATGATTACCGGCGCGGCCCAGATGGACGGCGCCATTCTGGTGGTGAG
CGCTCCCGATGGCCCGATGCCGCAGACCCGCGAGCACATTCTGCTCGCCCGCCAGGTGCAGGTGCCGGCCATCGTGGTCT
TCCTGAACAAGGTCGATATGATGGACGACCCGGAGCTGCTGGAGCTGGTCGAGCTGGAGCTGCGCGAGCTGCTTAGCAAG
TACGGCTTCCCGGGTGATGAGATTCCGATTGTGCGCGGCAGCGCCCGCAACGCGCTGGAAAGCCCGAGCAAGGACATCAA
CGCCCCAGAGTACAAATGTATTCTGGAGCTGATGAATGCGGTGGACGAGTACATTCCAACGCCGCAGCGGGCAGTGGATC
AGCCGTTCCTGATGCCGATTGAGGACGTGTTCGGGATTAAGGGCCGTGGCACGGTGGTGACGGGCCGGATCGAGCGCGGC
AAGGTGAAGGTCGGTGACACGGTTGAGATCGTGGGCATGACCAACGACGCGCCGCGGCGGACGGTGGTGACCGGTGTAGA
GATGTTCCAGAAGACGCTGGACGAAGGGATTGCCGGTGACAACGTCGGCTGTCTGCTGCGTGGCATTGAGCGCACCGATG
TGGAGCGCGGGCAGGTGTTGTGCGCACCGGGCAGCATCAAGCCGCACAAGAAGTTCGAGGCGCAGGTCTACGTGTTGAAG
AAGGAAGAGGGTGGCCGCCACACGCCATTCTTCTCGGGGTACCGCCCGCAGTTCTACATCCGCACGACCGACGTGACGGG
GGCGATTGGTCTGCCGGCGGGGATGGAGATGGTGATGCCGGGCGATAACGTGGTGATGACGATTGAGCTGATCGTCCCGG
TGGCTATCGAAGAGGGCTTGCGCTTCGCTATCCGCGAAGGTGGCCGCACTGTCGGTGCTGGTGTCGTAACCAAGATCCTG
GATTAG

Upstream 100 bases:

>100_bases
CCAACCGCTATCTACGCCGCCTGGTCTGATCGGGAAATAGCCATCTCTGGCTGATGATAGGGATTAAGTAGATCGCGCAA
GAACGCGGAGGAATCAAACA

Downstream 100 bases:

>100_bases
CATAGCGTAAACCGGTGACGGGGGCGTGGTCTCCACCACGTCCCTGTTGATTGGAGAAAAAGATGGCTAAACAGAAAGTT
CGTATTCGCCTGAAGGCGTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKQKFERTKPHINVGTIGHVDHGKTTLTAAITKVLSLKGAAQFMAYDQIDNAPEERARGITIAIRHVEYQTDKRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVQVPAIVVFLNKVDMMDDPELLELVELELRELLSK
YGFPGDEIPIVRGSARNALESPSKDINAPEYKCILELMNAVDEYIPTPQRAVDQPFLMPIEDVFGIKGRGTVVTGRIERG
KVKVGDTVEIVGMTNDAPRRTVVTGVEMFQKTLDEGIAGDNVGCLLRGIERTDVERGQVLCAPGSIKPHKKFEAQVYVLK
KEEGGRHTPFFSGYRPQFYIRTTDVTGAIGLPAGMEMVMPGDNVVMTIELIVPVAIEEGLRFAIREGGRTVGAGVVTKIL
D

Sequences:

>Translated_401_residues
MAKQKFERTKPHINVGTIGHVDHGKTTLTAAITKVLSLKGAAQFMAYDQIDNAPEERARGITIAIRHVEYQTDKRHYAHV
DCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVQVPAIVVFLNKVDMMDDPELLELVELELRELLSK
YGFPGDEIPIVRGSARNALESPSKDINAPEYKCILELMNAVDEYIPTPQRAVDQPFLMPIEDVFGIKGRGTVVTGRIERG
KVKVGDTVEIVGMTNDAPRRTVVTGVEMFQKTLDEGIAGDNVGCLLRGIERTDVERGQVLCAPGSIKPHKKFEAQVYVLK
KEEGGRHTPFFSGYRPQFYIRTTDVTGAIGLPAGMEMVMPGDNVVMTIELIVPVAIEEGLRFAIREGGRTVGAGVVTKIL
D
>Mature_400_residues
AKQKFERTKPHINVGTIGHVDHGKTTLTAAITKVLSLKGAAQFMAYDQIDNAPEERARGITIAIRHVEYQTDKRHYAHVD
CPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVQVPAIVVFLNKVDMMDDPELLELVELELRELLSKY
GFPGDEIPIVRGSARNALESPSKDINAPEYKCILELMNAVDEYIPTPQRAVDQPFLMPIEDVFGIKGRGTVVTGRIERGK
VKVGDTVEIVGMTNDAPRRTVVTGVEMFQKTLDEGIAGDNVGCLLRGIERTDVERGQVLCAPGSIKPHKKFEAQVYVLKK
EEGGRHTPFFSGYRPQFYIRTTDVTGAIGLPAGMEMVMPGDNVVMTIELIVPVAIEEGLRFAIREGGRTVGAGVVTKILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=56.6416040100251, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.1123595505618, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.2961275626424, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=30.2690582959641, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=29.3064876957494, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI194018522, Length=440, Percent_Identity=28.6363636363636, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI194097354, Length=440, Percent_Identity=28.6363636363636, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI194018520, Length=440, Percent_Identity=28.6363636363636, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI46094014, Length=440, Percent_Identity=27.9545454545455, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI54607086, Length=364, Percent_Identity=29.6703296703297, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI82546879, Length=339, Percent_Identity=25.9587020648968, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157426893, Length=296, Percent_Identity=25.6756756756757, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI45593140, Length=429, Percent_Identity=22.8438228438228, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=400, Percent_Identity=71.25, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1789737, Length=400, Percent_Identity=71.25, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI2367247, Length=342, Percent_Identity=29.8245614035088, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789108, Length=268, Percent_Identity=30.5970149253731, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=278, Percent_Identity=33.0935251798561, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788922, Length=340, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=52.3456790123457, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=394, Percent_Identity=42.1319796954315, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.3571428571429, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.3571428571429, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=28.4463894967177, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=29.6803652968037, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=442, Percent_Identity=28.2805429864253, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=28.246013667426, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17506081, Length=349, Percent_Identity=26.647564469914, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=37.5838926174497, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=27.8688524590164, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=447, Percent_Identity=24.3847874720358, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=63.5, Blast_Score=522, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=30.2895322939866, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=30.2895322939866, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=453, Percent_Identity=26.9315673289183, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322937, Length=363, Percent_Identity=26.1707988980716, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=28.6231884057971, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=248, Percent_Identity=28.2258064516129, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=32.1637426900585, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281363316, Length=398, Percent_Identity=58.0402010050251, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=398, Percent_Identity=58.0402010050251, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=393, Percent_Identity=49.8727735368957, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30.6666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30.6666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30.6666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30.6666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.2013422818792, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.2013422818792, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=32.463768115942, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19922690, Length=310, Percent_Identity=32.258064516129, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI45550900, Length=449, Percent_Identity=28.9532293986637, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24647048, Length=454, Percent_Identity=24.2290748898678, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24647046, Length=454, Percent_Identity=24.2290748898678, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI78706572, Length=245, Percent_Identity=30.2040816326531, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28572034, Length=127, Percent_Identity=44.0944881889764, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CHLAA (A9WFP3)

Other databases:

- EMBL:   CP000909
- EMBL:   CP000909
- RefSeq:   YP_001635782.1
- RefSeq:   YP_001635965.1
- ProteinModelPortal:   A9WFP3
- SMR:   A9WFP3
- GeneID:   5826640
- GeneID:   5826831
- GenomeReviews:   CP000909_GR
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2182
- KEGG:   cau:Caur_2367
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44020; Mature: 43888

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00301 EFACTOR_GTP; PS00467 RIBOSOMAL_L2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MAKQKFERTKPHINVGTIGHVDHGKTTLTAAITKVLSLKGAAQFMAYDQIDNAPEERARG
CCCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCC
ITIAIRHVEYQTDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVQVPAIVVFLNKVDMMDDPELLELVELELRELLSKYGFPGDEIPIVRGSARNALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHC
SPSKDINAPEYKCILELMNAVDEYIPTPQRAVDQPFLMPIEDVFGIKGRGTVVTGRIERG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCEEEEEEEECC
KVKVGDTVEIVGMTNDAPRRTVVTGVEMFQKTLDEGIAGDNVGCLLRGIERTDVERGQVL
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCEEE
CAPGSIKPHKKFEAQVYVLKKEEGGRHTPFFSGYRPQFYIRTTDVTGAIGLPAGMEMVMP
ECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEC
GDNVVMTIELIVPVAIEEGLRFAIREGGRTVGAGVVTKILD
CCCEEEEEEEEEEHHHHHCHHHHHHCCCCEECHHHHHHHCC
>Mature Secondary Structure 
AKQKFERTKPHINVGTIGHVDHGKTTLTAAITKVLSLKGAAQFMAYDQIDNAPEERARG
CCCHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCC
ITIAIRHVEYQTDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVQVPAIVVFLNKVDMMDDPELLELVELELRELLSKYGFPGDEIPIVRGSARNALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHC
SPSKDINAPEYKCILELMNAVDEYIPTPQRAVDQPFLMPIEDVFGIKGRGTVVTGRIERG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHCCCCCCCEEEEEEEECC
KVKVGDTVEIVGMTNDAPRRTVVTGVEMFQKTLDEGIAGDNVGCLLRGIERTDVERGQVL
EEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCEEE
CAPGSIKPHKKFEAQVYVLKKEEGGRHTPFFSGYRPQFYIRTTDVTGAIGLPAGMEMVMP
ECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEC
GDNVVMTIELIVPVAIEEGLRFAIREGGRTVGAGVVTKILD
CCCEEEEEEEEEEHHHHHCHHHHHHCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA