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The map label for this gene is murE [H]

Identifier: 222525769

GI number: 222525769

Start: 3169651

End: 3171180

Strand: Reverse

Name: murE [H]

Synonym: Chy400_2522

Alternate gene names: 222525769

Gene position: 3171180-3169651 (Counterclockwise)

Preceding gene: 222525770

Following gene: 222525768

Centisome position: 60.19

GC content: 59.61

Gene sequence:

>1530_bases
ATGACGGCAAAAACACTTGCCAGTCTCCTGAACAATGTAGCTGTCCAACGGCTAATTGGCGAACCACTCGTGCCTGTGCA
ATCGCTCACCTACGACTCGCGGCGTGTTGAGCCGGGGAGTCTGTTTGTTGCCATCCGTGGGCAGCACAGTGATGGTCACC
AGTTTATTGACCAGGCTATCGCACGGGGAGCTGTGGCCGTGGTGGTCGATCAACGGTATTGGGATGAACGACCGGTTGCC
GTGCCGGTAGTGGTGGTCGCCGATAGCCGGGTGGCCCTGGCACCACTGGCGGCTGCGTTCTACGACTATCCCGGACGTGA
ATTGACCACAATCGGGATTACCGGTACCAAAGGAAAAAGCACAACGACCGATGTGACGGCCCAGCTCCTCGCTGCTACCG
GACGTATGGTGGGCATGATCAGCACCGTCGATTTTCAGATTGGGAATCGCCGCTGGCCTAACGATACCCGTCAGAGCACA
CCGGAAGCACCAGAGGTGCAGGCATTGCTGCGCGAGATGGTCACTGCCGGTTGCGATACAGCCGTTATTGAAGCGACATC
CCACGCTCTCTCGCCACGCTGGGGGCGGCTGGTTGGCTGTGCGTTCGCTGTGGCTGTAATGCTTAATATCGGCCACGAAC
ATCTCGATTACCACGGCACATTCGAGCAGTATCGGGCCGACAAAGCCCAGCTCTTTGCGCTACTTGGCGAACGCAGTGGT
TCGCGCTGGGCAATTGTCAATGCTGACGATCCAAATCATGCGTATTTCCTTGATGCTGCACCTGCCGATGCGGTGCGGCT
ACGGTATGGTTTACACGAGCCGGCAGATGTCCAGGGACAGATACTCCACAGTGGGCCGATGAGCAGTGTGGTGCGGGTAA
CGTCACCCTGGGGCACCACCGATCTGACCGTTCCGCTACCGGGGCGATTCAACGCCAGTAATGCGCTGGCCGCGCTGACA
GTCGCTCTCAGTCAGGGCGTTGCGCTCGAGGCAGCCGCTGCCGCCCTGGCCGGCGTGCGGGCACCACGTGGCCGGATGGT
GCCAATTGATGCCGGGCAACCATTCGCGGTGATTGTAGACTATGCTCATAATCCCGATTCGTTCGAGCAGATCTTTGCCA
TGCTGCGGCCCCAGGTGCAGGGGCGAATGATCGCGGTTTTCGGCAGTGCCGGCGAGCGTGATGTTGCCAAACGGGCTATT
CAGGGAGAAATTGCCGGTCGGATGTGTGACTTGCTGGTGCTGACCGATGAAGATCCACGTGGCGAGGATCGCGAGGCAAT
CATTGCCCAGATCGCTGCCGGTGCCGAACGGGCCGGCAAACGACCCGGTAGTGGGTACCTCTGTATTCCTGATCGGGCAC
AGGCCATTCGGGCTGCCATGGCCGCCGCACAACCCGGTGACCTGGTGTTGTTGCTGGGCAAAGGACATGAGGGTAGCATT
ATCTACGCCGACTTTTCACTGCCGTGGAACGAAGAGCGCGAAGCCCGGCAGGCACTGGCCGAGTTAGGATACCATCGGTC
GCATGATTGA

Upstream 100 bases:

>100_bases
GTTTTGGTGGTCGAATCGTTCGTTTTATGCCGGCTTTCGATCTGGTCCTGTTGCCATTACTCTATCCATTGGCGCGACGA
AAGATTGGAGGATAACGTCA

Downstream 100 bases:

>100_bases
ACTGGCACCACACAATCCGTATGGCCTGACGATTGCTTCACCGGTCATTGCGGCTGCCGGCAGCCTGGGTGACGTGGTCG
GTGTAGCGCGCTGGTTAGGG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTAKTLASLLNNVAVQRLIGEPLVPVQSLTYDSRRVEPGSLFVAIRGQHSDGHQFIDQAIARGAVAVVVDQRYWDERPVA
VPVVVVADSRVALAPLAAAFYDYPGRELTTIGITGTKGKSTTTDVTAQLLAATGRMVGMISTVDFQIGNRRWPNDTRQST
PEAPEVQALLREMVTAGCDTAVIEATSHALSPRWGRLVGCAFAVAVMLNIGHEHLDYHGTFEQYRADKAQLFALLGERSG
SRWAIVNADDPNHAYFLDAAPADAVRLRYGLHEPADVQGQILHSGPMSSVVRVTSPWGTTDLTVPLPGRFNASNALAALT
VALSQGVALEAAAAALAGVRAPRGRMVPIDAGQPFAVIVDYAHNPDSFEQIFAMLRPQVQGRMIAVFGSAGERDVAKRAI
QGEIAGRMCDLLVLTDEDPRGEDREAIIAQIAAGAERAGKRPGSGYLCIPDRAQAIRAAMAAAQPGDLVLLLGKGHEGSI
IYADFSLPWNEEREARQALAELGYHRSHD

Sequences:

>Translated_509_residues
MTAKTLASLLNNVAVQRLIGEPLVPVQSLTYDSRRVEPGSLFVAIRGQHSDGHQFIDQAIARGAVAVVVDQRYWDERPVA
VPVVVVADSRVALAPLAAAFYDYPGRELTTIGITGTKGKSTTTDVTAQLLAATGRMVGMISTVDFQIGNRRWPNDTRQST
PEAPEVQALLREMVTAGCDTAVIEATSHALSPRWGRLVGCAFAVAVMLNIGHEHLDYHGTFEQYRADKAQLFALLGERSG
SRWAIVNADDPNHAYFLDAAPADAVRLRYGLHEPADVQGQILHSGPMSSVVRVTSPWGTTDLTVPLPGRFNASNALAALT
VALSQGVALEAAAAALAGVRAPRGRMVPIDAGQPFAVIVDYAHNPDSFEQIFAMLRPQVQGRMIAVFGSAGERDVAKRAI
QGEIAGRMCDLLVLTDEDPRGEDREAIIAQIAAGAERAGKRPGSGYLCIPDRAQAIRAAMAAAQPGDLVLLLGKGHEGSI
IYADFSLPWNEEREARQALAELGYHRSHD
>Mature_508_residues
TAKTLASLLNNVAVQRLIGEPLVPVQSLTYDSRRVEPGSLFVAIRGQHSDGHQFIDQAIARGAVAVVVDQRYWDERPVAV
PVVVVADSRVALAPLAAAFYDYPGRELTTIGITGTKGKSTTTDVTAQLLAATGRMVGMISTVDFQIGNRRWPNDTRQSTP
EAPEVQALLREMVTAGCDTAVIEATSHALSPRWGRLVGCAFAVAVMLNIGHEHLDYHGTFEQYRADKAQLFALLGERSGS
RWAIVNADDPNHAYFLDAAPADAVRLRYGLHEPADVQGQILHSGPMSSVVRVTSPWGTTDLTVPLPGRFNASNALAALTV
ALSQGVALEAAAAALAGVRAPRGRMVPIDAGQPFAVIVDYAHNPDSFEQIFAMLRPQVQGRMIAVFGSAGERDVAKRAIQ
GEIAGRMCDLLVLTDEDPRGEDREAIIAQIAAGAERAGKRPGSGYLCIPDRAQAIRAAMAAAQPGDLVLLLGKGHEGSII
YADFSLPWNEEREARQALAELGYHRSHD

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=33.0595482546201, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786274, Length=347, Percent_Identity=28.5302593659942, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 54584; Mature: 54452

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTLASLLNNVAVQRLIGEPLVPVQSLTYDSRRVEPGSLFVAIRGQHSDGHQFIDQAI
CCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGAVAVVVDQRYWDERPVAVPVVVVADSRVALAPLAAAFYDYPGRELTTIGITGTKGKS
HCCEEEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
TTTDVTAQLLAATGRMVGMISTVDFQIGNRRWPNDTRQSTPEAPEVQALLREMVTAGCDT
CHHHHHHHHHHHCCHHEEEEEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCC
AVIEATSHALSPRWGRLVGCAFAVAVMLNIGHEHLDYHGTFEQYRADKAQLFALLGERSG
EEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHCHHHHHHHHHHCCCC
SRWAIVNADDPNHAYFLDAAPADAVRLRYGLHEPADVQGQILHSGPMSSVVRVTSPWGTT
CEEEEEECCCCCCEEEEECCCCCHHHHEECCCCCCCCCCEEEECCCCCCEEEEECCCCCC
DLTVPLPGRFNASNALAALTVALSQGVALEAAAAALAGVRAPRGRMVPIDAGQPFAVIVD
EEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEE
YAHNPDSFEQIFAMLRPQVQGRMIAVFGSAGERDVAKRAIQGEIAGRMCDLLVLTDEDPR
CCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCC
GEDREAIIAQIAAGAERAGKRPGSGYLCIPDRAQAIRAAMAAAQPGDLVLLLGKGHEGSI
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCE
IYADFSLPWNEEREARQALAELGYHRSHD
EEEEECCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TAKTLASLLNNVAVQRLIGEPLVPVQSLTYDSRRVEPGSLFVAIRGQHSDGHQFIDQAI
CHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGAVAVVVDQRYWDERPVAVPVVVVADSRVALAPLAAAFYDYPGRELTTIGITGTKGKS
HCCEEEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCC
TTTDVTAQLLAATGRMVGMISTVDFQIGNRRWPNDTRQSTPEAPEVQALLREMVTAGCDT
CHHHHHHHHHHHCCHHEEEEEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCC
AVIEATSHALSPRWGRLVGCAFAVAVMLNIGHEHLDYHGTFEQYRADKAQLFALLGERSG
EEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHCHHHHHHHHHHCCCC
SRWAIVNADDPNHAYFLDAAPADAVRLRYGLHEPADVQGQILHSGPMSSVVRVTSPWGTT
CEEEEEECCCCCCEEEEECCCCCHHHHEECCCCCCCCCCEEEECCCCCCEEEEECCCCCC
DLTVPLPGRFNASNALAALTVALSQGVALEAAAAALAGVRAPRGRMVPIDAGQPFAVIVD
EEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEE
YAHNPDSFEQIFAMLRPQVQGRMIAVFGSAGERDVAKRAIQGEIAGRMCDLLVLTDEDPR
CCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCC
GEDREAIIAQIAAGAERAGKRPGSGYLCIPDRAQAIRAAMAAAQPGDLVLLLGKGHEGSI
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCE
IYADFSLPWNEEREARQALAELGYHRSHD
EEEEECCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA