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The map label for this gene is pckG [H]

Identifier: 222525760

GI number: 222525760

Start: 3157089

End: 3158924

Strand: Reverse

Name: pckG [H]

Synonym: Chy400_2513

Alternate gene names: 222525760

Gene position: 3158924-3157089 (Counterclockwise)

Preceding gene: 222525764

Following gene: 222525759

Centisome position: 59.95

GC content: 53.27

Gene sequence:

>1836_bases
ATGCAATTTCCGGCATACATTAAACACCACCGGTTACGCGAGTGGATTTCAGACATTGTCGCACTCTGCGAACCGGCACA
AGTTCATTTTTGTGACGGAACACCGGCAGAGTACGACACGCTCTGCAACCAGATGGTAGAAAACGGAACATTCATTCGAC
TCAATCCCAAACTACGCCCCAACAGCTTTCTCGCCCGCTCAGACCCCTCAGATGTTGCTCGCGTTGAAGATCGTACCTTC
ATCTGTAGCATCTCGAAAGACGATGCTGGCCCCACTAATAACTGGATGCCGCCGAAAGAGATGAAGGAAATCCTGCTCAA
ATTGTTTAAAGGGAGTATGCGGGGCCGTGTCCTGTACGTTATTCCCTTCAGCATGGGGCCAATCGGATCACCCATTGCCC
ACATTGGTATTCAATTAACCGACTCGCCATATGTCGTGGTCAACATGCACATAATGACCCGCGTCTCAACTAAAATTTAC
GACGTATTGGGTGAGGACGGCGAGTACATCCCATGCCTCCATAGCGTTGGAATGCCGCTGGAACCGGGTCAGCCCGACGT
ACCCTGGCCCTGCAATCCAACCAAATATATTGTTCACTTTCCCGAAGAGCGTTCGATCTGGAGTTTCGGATCGGGGTATG
GCGGTAATGCTCTGCTTGGTAAAAAGTGCTTCGCATTGCGGATTGCGTCGGTCATGGCCCGGCAAGAGGGCTGGCTTGCC
GAACACATGTTGATCCTGGGAGTAAAAGAACCATCTGGACGCAAGACCTATGTAGCGGCAGCGTTCCCCTCGGCATGCGG
AAAAACCAATTTTGCCATGCTCATCCCGCCTGACACCTTCAAGCGAGAGGGATGGGAAATTACAACCGTCGGCGACGACA
TCGCCTGGATCAAACCCGGTGCAAATGGCAAACTCTACGCAATCAATCCTGAAGCAGGGTATTTTGGTGTTGCGCCGGGC
ACATCATATAAAACCAATCCCAATGCGATGGAAAGCTGCCGCGCCAATACCATCTTCACCAATGTTGCGCTCACCGATGA
CGGCGATGTCTGGTGGGAAGGCATGACCGATGAACCACCGGCACACCTGATCGACTGGCAGGGGCGTGATTGGACACCGG
GATGTGGCCGCCTGGCAGCCCATCCGAATGCACGTTTTACAGCACCGGCCAGGCAGAACCCGGCCATTGATCCGGAGTGG
GAAAATCCGAATGGGGTGCCGATTGAGGCTTTCATTTTCGGTGGTCGACGTAGCCGGGTGGTGCCACTGGTCTATCAGGC
GTTCAACTGGACCTATGGTGTGTATCTGGCCGCCACGATGGGTTCAGAAACAACCGCAGCAGCGATGGGGCAACAGGGTG
TTGTTCGCCGTGATCCGTTTGCAATGCTACCATTCGCCGGCTACCATATGGGCGACTACTTCAATCACTGGCTGAGTTTT
GGCCGTCGCTTGCGCAATCCACCGCGAATCTTCAGCGTCAACTGGTTCCGCAAAGATGAAAACGGTAAGTTTATCTGGCC
AGGTTTTGGCGAGAATATGCGAGTGTTAAAGTGGATCATCGAACGTGCCCACGGCCAGGCCGTCAGTGTCGAAACACCGA
TTGGCTGGGTACCACGGTATGAAGACATCGACTGGCGTGGTCTCAGCTTTAGCGCCGAGCAGTTTGAACAGGTGATGGAG
TGTAACGTCGGTGAGTGGCAGCAAGAGATATTACTGCACGAAGAACTGTTCATCAAACTATACGACCGTCTCCCGAAAGA
ACTGTTATCGGTACGCGACTTAATCCTTTCGAGCCTCTGGCGTATGCCTGAGAAATGGGGAGCGACCCACGAATAA

Upstream 100 bases:

>100_bases
CTGCACTCATCATTGTTTCTCGCCATGTTATGATTATCATTGTTCAATGTGTCACAGTCATCGCCCGCCAATGGTGCCGT
GAGCGAAAGGAAGGCTTCGT

Downstream 100 bases:

>100_bases
CACCTGTACAATGTGAACATAGGAGGGGGCGGCGTGCGCAACATGTACGCCGCCCCTAAATAAATATAAATATCAGGTTT
ACAAGCATCCGCGAGCTATC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MQFPAYIKHHRLREWISDIVALCEPAQVHFCDGTPAEYDTLCNQMVENGTFIRLNPKLRPNSFLARSDPSDVARVEDRTF
ICSISKDDAGPTNNWMPPKEMKEILLKLFKGSMRGRVLYVIPFSMGPIGSPIAHIGIQLTDSPYVVVNMHIMTRVSTKIY
DVLGEDGEYIPCLHSVGMPLEPGQPDVPWPCNPTKYIVHFPEERSIWSFGSGYGGNALLGKKCFALRIASVMARQEGWLA
EHMLILGVKEPSGRKTYVAAAFPSACGKTNFAMLIPPDTFKREGWEITTVGDDIAWIKPGANGKLYAINPEAGYFGVAPG
TSYKTNPNAMESCRANTIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPGCGRLAAHPNARFTAPARQNPAIDPEW
ENPNGVPIEAFIFGGRRSRVVPLVYQAFNWTYGVYLAATMGSETTAAAMGQQGVVRRDPFAMLPFAGYHMGDYFNHWLSF
GRRLRNPPRIFSVNWFRKDENGKFIWPGFGENMRVLKWIIERAHGQAVSVETPIGWVPRYEDIDWRGLSFSAEQFEQVME
CNVGEWQQEILLHEELFIKLYDRLPKELLSVRDLILSSLWRMPEKWGATHE

Sequences:

>Translated_611_residues
MQFPAYIKHHRLREWISDIVALCEPAQVHFCDGTPAEYDTLCNQMVENGTFIRLNPKLRPNSFLARSDPSDVARVEDRTF
ICSISKDDAGPTNNWMPPKEMKEILLKLFKGSMRGRVLYVIPFSMGPIGSPIAHIGIQLTDSPYVVVNMHIMTRVSTKIY
DVLGEDGEYIPCLHSVGMPLEPGQPDVPWPCNPTKYIVHFPEERSIWSFGSGYGGNALLGKKCFALRIASVMARQEGWLA
EHMLILGVKEPSGRKTYVAAAFPSACGKTNFAMLIPPDTFKREGWEITTVGDDIAWIKPGANGKLYAINPEAGYFGVAPG
TSYKTNPNAMESCRANTIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPGCGRLAAHPNARFTAPARQNPAIDPEW
ENPNGVPIEAFIFGGRRSRVVPLVYQAFNWTYGVYLAATMGSETTAAAMGQQGVVRRDPFAMLPFAGYHMGDYFNHWLSF
GRRLRNPPRIFSVNWFRKDENGKFIWPGFGENMRVLKWIIERAHGQAVSVETPIGWVPRYEDIDWRGLSFSAEQFEQVME
CNVGEWQQEILLHEELFIKLYDRLPKELLSVRDLILSSLWRMPEKWGATHE
>Mature_611_residues
MQFPAYIKHHRLREWISDIVALCEPAQVHFCDGTPAEYDTLCNQMVENGTFIRLNPKLRPNSFLARSDPSDVARVEDRTF
ICSISKDDAGPTNNWMPPKEMKEILLKLFKGSMRGRVLYVIPFSMGPIGSPIAHIGIQLTDSPYVVVNMHIMTRVSTKIY
DVLGEDGEYIPCLHSVGMPLEPGQPDVPWPCNPTKYIVHFPEERSIWSFGSGYGGNALLGKKCFALRIASVMARQEGWLA
EHMLILGVKEPSGRKTYVAAAFPSACGKTNFAMLIPPDTFKREGWEITTVGDDIAWIKPGANGKLYAINPEAGYFGVAPG
TSYKTNPNAMESCRANTIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPGCGRLAAHPNARFTAPARQNPAIDPEW
ENPNGVPIEAFIFGGRRSRVVPLVYQAFNWTYGVYLAATMGSETTAAAMGQQGVVRRDPFAMLPFAGYHMGDYFNHWLSF
GRRLRNPPRIFSVNWFRKDENGKFIWPGFGENMRVLKWIIERAHGQAVSVETPIGWVPRYEDIDWRGLSFSAEQFEQVME
CNVGEWQQEILLHEELFIKLYDRLPKELLSVRDLILSSLWRMPEKWGATHE

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=606, Percent_Identity=51.8151815181518, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI187281517, Length=607, Percent_Identity=49.9176276771005, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI66346723, Length=379, Percent_Identity=53.8258575197889, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17555492, Length=563, Percent_Identity=51.1545293072824, Blast_Score=566, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989645, Length=574, Percent_Identity=49.6515679442509, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989658, Length=574, Percent_Identity=49.6515679442509, Blast_Score=560, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989653, Length=560, Percent_Identity=50.5357142857143, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=538, Percent_Identity=51.8587360594795, Blast_Score=551, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32564407, Length=471, Percent_Identity=55.2016985138004, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71989649, Length=484, Percent_Identity=50.4132231404959, Blast_Score=485, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564409, Length=317, Percent_Identity=54.2586750788643, Blast_Score=342, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17540956, Length=480, Percent_Identity=25, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24655085, Length=572, Percent_Identity=50.8741258741259, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24655082, Length=579, Percent_Identity=50.259067357513, Blast_Score=567, Evalue=1e-162,
Organism=Drosophila melanogaster, GI161077226, Length=546, Percent_Identity=51.0989010989011, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24655088, Length=474, Percent_Identity=52.9535864978903, Blast_Score=500, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 69071; Mature: 69071

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPAYIKHHRLREWISDIVALCEPAQVHFCDGTPAEYDTLCNQMVENGTFIRLNPKLRP
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCC
NSFLARSDPSDVARVEDRTFICSISKDDAGPTNNWMPPKEMKEILLKLFKGSMRGRVLYV
CCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEE
IPFSMGPIGSPIAHIGIQLTDSPYVVVNMHIMTRVSTKIYDVLGEDGEYIPCLHSVGMPL
EECCCCCCCCCHHHEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCCEECHHHHCCCCC
EPGQPDVPWPCNPTKYIVHFPEERSIWSFGSGYGGNALLGKKCFALRIASVMARQEGWLA
CCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
EHMLILGVKEPSGRKTYVAAAFPSACGKTNFAMLIPPDTFKREGWEITTVGDDIAWIKPG
EEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCC
ANGKLYAINPEAGYFGVAPGTSYKTNPNAMESCRANTIFTNVALTDDGDVWWEGMTDEPP
CCCEEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCEEECCCCCCCC
AHLIDWQGRDWTPGCGRLAAHPNARFTAPARQNPAIDPEWENPNGVPIEAFIFGGRRSRV
CCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
VPLVYQAFNWTYGVYLAATMGSETTAAAMGQQGVVRRDPFAMLPFAGYHMGDYFNHWLSF
EHEEHHHHCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHH
GRRLRNPPRIFSVNWFRKDENGKFIWPGFGENMRVLKWIIERAHGQAVSVETPIGWVPRY
HHHHCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
EDIDWRGLSFSAEQFEQVMECNVGEWQQEILLHEELFIKLYDRLPKELLSVRDLILSSLW
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMPEKWGATHE
CCHHHCCCCCC
>Mature Secondary Structure
MQFPAYIKHHRLREWISDIVALCEPAQVHFCDGTPAEYDTLCNQMVENGTFIRLNPKLRP
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCC
NSFLARSDPSDVARVEDRTFICSISKDDAGPTNNWMPPKEMKEILLKLFKGSMRGRVLYV
CCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEE
IPFSMGPIGSPIAHIGIQLTDSPYVVVNMHIMTRVSTKIYDVLGEDGEYIPCLHSVGMPL
EECCCCCCCCCHHHEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCCEECHHHHCCCCC
EPGQPDVPWPCNPTKYIVHFPEERSIWSFGSGYGGNALLGKKCFALRIASVMARQEGWLA
CCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
EHMLILGVKEPSGRKTYVAAAFPSACGKTNFAMLIPPDTFKREGWEITTVGDDIAWIKPG
EEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCC
ANGKLYAINPEAGYFGVAPGTSYKTNPNAMESCRANTIFTNVALTDDGDVWWEGMTDEPP
CCCEEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCCEEECCCCCCCC
AHLIDWQGRDWTPGCGRLAAHPNARFTAPARQNPAIDPEWENPNGVPIEAFIFGGRRSRV
CCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
VPLVYQAFNWTYGVYLAATMGSETTAAAMGQQGVVRRDPFAMLPFAGYHMGDYFNHWLSF
EHEEHHHHCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHH
GRRLRNPPRIFSVNWFRKDENGKFIWPGFGENMRVLKWIIERAHGQAVSVETPIGWVPRY
HHHHCCCCCEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
EDIDWRGLSFSAEQFEQVMECNVGEWQQEILLHEELFIKLYDRLPKELLSVRDLILSSLW
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMPEKWGATHE
CCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA