Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 222525749

GI number: 222525749

Start: 3140348

End: 3142117

Strand: Reverse

Name: aspS [H]

Synonym: Chy400_2500

Alternate gene names: 222525749

Gene position: 3142117-3140348 (Counterclockwise)

Preceding gene: 222525750

Following gene: 222525748

Centisome position: 59.63

GC content: 56.95

Gene sequence:

>1770_bases
ATGTACCGTTCACATACCTGTGGTGAGTTACGTCCGGCACACGCCGGTCAGGAGGTGACGCTGGCCGGCTGGGTTCATCG
TCGGCGCGACCACGGCGATCTCATCTTCATCGACCTGCGTGATCGCTATGGGATCACGCAGGTTGTTTTCAACAGCGCCC
ATCCAACCGCCCACGAGGTTGCCGAAACTGTTCGCTCGGAATATGTGCTTCAGGTTCGCGGGAAGGTACGGATTCGCCCC
CCAGAGGCGGTGAATCCTGACCTGGCCACCGGCGAGATCGAGCTGGAAGCGTCGGAAGCACAGGTGCTGAATCCGGCCCG
CACACCACCCATTTATATTGCTAAAGAGGGTGGTGAAGACGAGTCGGTGCGATTGAAGTACCGGTATCTCGATCTACGGC
GCGAACGGATGCAGCGCAACCTGATCTTACGCCATCGCGTCGTCAAATTCATTCGTGACTTCCTCGATGCGGAAGGTTTT
CTTGAAATTGAGACACCAATTTTGATCAAATCGACCCCGGAAGGTGCGCGTGACTACCTGGTGCCGTCGCGATTGCATCC
GGGCAAGTTCTACGCCCTGCCGCAAAGCCCACAGCAACTGAAGCAACTGTTGATGGTTGCCGGTTACGACAAATATTTTC
AGATTGCCCGTTGTTTTCGTGACGAAGACCAGCGTGCCGACCGGCAACCGGAATTCACGCAGCTCGACATGGAGATGAGC
TTCGTCGATCAAGACGATGTGCTTGATCTGATCGAGCGCATGTTCACCGCCCTCTGTCGCACCGTCGTCCCCCACAAACA
TCTGCCCACACCCTTTCGGCGGTTGTCGTATGCGGAAGCGATGGAGCGCTACGGCTCGGATAAACCAGACCTGCGCTACG
GTTTGGAGCTGGTCAACCTGAGCGATCTGCTGATCGAGACACCATTCCAGGTCTTTCGCAATGTGTTGAACAGTGGTGGG
CAGGTCAAAGGTATTCGAGCACCGGGCTGTGCCCACTTCTCGCGCAAGCAGATTGATGAGTTGACCGATGTGGTACGGGC
TGGTGGCGCTAAAGGGTTAGCCTGGGCAGTATTACCGACAGATGGTGGTGAGGTACGGTCGAGCTTTGCCAAAAACCTGG
CACCCGGCGAGTTTGCTGCTATCGTCGAACGGATGCAGGCTGAAGCCGGCGATCTGCTCTTAATCGTCGCCGATCAACCG
GCAGTCGTTGCCGCTTCGCTCGACAAACTGCGTCGCAACCTGGCCGAACGGCTGCAACTGGCCGATCCGAATACGCTCTG
CTTCGCCTGGATCGTTGACTTCCCGCTAGTGGAATGGAACGAAGACGAGCAACGTTGGGATGCCGTTCACCATCCATTTA
CGGCACCCAAAGATGAAGACATGCACCTGCTGGCAACCGATCCCGGCAAGGTACGGGCGAAGGCTTACGATCTCATCCTC
AACGGCTATGAAGCCGGTGGCGGCAGCATCCGTATTCATCGCCGCGATGTTCAGCAACAGATCTTCTCGCTGCTGGGGAT
TAGCGAAGAGCAGGCCCAGGCGCAGTTTGGGCACATGCTCGAAGCCTTTGAATACGGTGCACCTCCCCACGGTGGCATTG
CCCCCGGCATTGATCGCCTGGTGATGATTCTGGCCGATGAACCGACCATTCGCGAGGTGATGGCCTTCCCCAAGACCCAG
CAAGCGGTTGATTTGATGACGAATGCACCATCGCCGGTGGATGAGCGGCAGTTGAAAGAGCTGCATATCAGGATCGTGAT
GCCAGAATAA

Upstream 100 bases:

>100_bases
CCAATTGATCGATGAGACTGGCGCATAAGAGACCTGAATGCCTGTGTTATAATGCGACGGCTAAGCAAGCAATGCTGCTT
TCAATTCGGCAGGAGTGTCT

Downstream 100 bases:

>100_bases
GCCGAGCCTGATAGTTGTGAAGTGCGAACGTATCACAAGCCTGCCGGGTGTAAAGCCCGGCAGATGTGTTGTAAGACGGC
AGGTGTGCAATAACACGATC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MYRSHTCGELRPAHAGQEVTLAGWVHRRRDHGDLIFIDLRDRYGITQVVFNSAHPTAHEVAETVRSEYVLQVRGKVRIRP
PEAVNPDLATGEIELEASEAQVLNPARTPPIYIAKEGGEDESVRLKYRYLDLRRERMQRNLILRHRVVKFIRDFLDAEGF
LEIETPILIKSTPEGARDYLVPSRLHPGKFYALPQSPQQLKQLLMVAGYDKYFQIARCFRDEDQRADRQPEFTQLDMEMS
FVDQDDVLDLIERMFTALCRTVVPHKHLPTPFRRLSYAEAMERYGSDKPDLRYGLELVNLSDLLIETPFQVFRNVLNSGG
QVKGIRAPGCAHFSRKQIDELTDVVRAGGAKGLAWAVLPTDGGEVRSSFAKNLAPGEFAAIVERMQAEAGDLLLIVADQP
AVVAASLDKLRRNLAERLQLADPNTLCFAWIVDFPLVEWNEDEQRWDAVHHPFTAPKDEDMHLLATDPGKVRAKAYDLIL
NGYEAGGGSIRIHRRDVQQQIFSLLGISEEQAQAQFGHMLEAFEYGAPPHGGIAPGIDRLVMILADEPTIREVMAFPKTQ
QAVDLMTNAPSPVDERQLKELHIRIVMPE

Sequences:

>Translated_589_residues
MYRSHTCGELRPAHAGQEVTLAGWVHRRRDHGDLIFIDLRDRYGITQVVFNSAHPTAHEVAETVRSEYVLQVRGKVRIRP
PEAVNPDLATGEIELEASEAQVLNPARTPPIYIAKEGGEDESVRLKYRYLDLRRERMQRNLILRHRVVKFIRDFLDAEGF
LEIETPILIKSTPEGARDYLVPSRLHPGKFYALPQSPQQLKQLLMVAGYDKYFQIARCFRDEDQRADRQPEFTQLDMEMS
FVDQDDVLDLIERMFTALCRTVVPHKHLPTPFRRLSYAEAMERYGSDKPDLRYGLELVNLSDLLIETPFQVFRNVLNSGG
QVKGIRAPGCAHFSRKQIDELTDVVRAGGAKGLAWAVLPTDGGEVRSSFAKNLAPGEFAAIVERMQAEAGDLLLIVADQP
AVVAASLDKLRRNLAERLQLADPNTLCFAWIVDFPLVEWNEDEQRWDAVHHPFTAPKDEDMHLLATDPGKVRAKAYDLIL
NGYEAGGGSIRIHRRDVQQQIFSLLGISEEQAQAQFGHMLEAFEYGAPPHGGIAPGIDRLVMILADEPTIREVMAFPKTQ
QAVDLMTNAPSPVDERQLKELHIRIVMPE
>Mature_589_residues
MYRSHTCGELRPAHAGQEVTLAGWVHRRRDHGDLIFIDLRDRYGITQVVFNSAHPTAHEVAETVRSEYVLQVRGKVRIRP
PEAVNPDLATGEIELEASEAQVLNPARTPPIYIAKEGGEDESVRLKYRYLDLRRERMQRNLILRHRVVKFIRDFLDAEGF
LEIETPILIKSTPEGARDYLVPSRLHPGKFYALPQSPQQLKQLLMVAGYDKYFQIARCFRDEDQRADRQPEFTQLDMEMS
FVDQDDVLDLIERMFTALCRTVVPHKHLPTPFRRLSYAEAMERYGSDKPDLRYGLELVNLSDLLIETPFQVFRNVLNSGG
QVKGIRAPGCAHFSRKQIDELTDVVRAGGAKGLAWAVLPTDGGEVRSSFAKNLAPGEFAAIVERMQAEAGDLLLIVADQP
AVVAASLDKLRRNLAERLQLADPNTLCFAWIVDFPLVEWNEDEQRWDAVHHPFTAPKDEDMHLLATDPGKVRAKAYDLIL
NGYEAGGGSIRIHRRDVQQQIFSLLGISEEQAQAQFGHMLEAFEYGAPPHGGIAPGIDRLVMILADEPTIREVMAFPKTQ
QAVDLMTNAPSPVDERQLKELHIRIVMPE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=42.6910299003322, Blast_Score=461, Evalue=1e-129,
Organism=Homo sapiens, GI5031815, Length=303, Percent_Identity=28.3828382838284, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI194272210, Length=303, Percent_Identity=28.3828382838284, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI4758762, Length=284, Percent_Identity=27.8169014084507, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI45439306, Length=306, Percent_Identity=27.1241830065359, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=47.7966101694915, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1789256, Length=321, Percent_Identity=26.791277258567, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=27.4914089347079, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=34.7826086956522, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI71984122, Length=288, Percent_Identity=28.8194444444444, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=295, Percent_Identity=25.4237288135593, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=249, Percent_Identity=26.5060240963855, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=249, Percent_Identity=26.5060240963855, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=274, Percent_Identity=23.7226277372263, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=645, Percent_Identity=33.6434108527132, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=300, Percent_Identity=25, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=310, Percent_Identity=27.741935483871, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=219, Percent_Identity=26.9406392694064, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=40.0335008375209, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640849, Length=296, Percent_Identity=27.7027027027027, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640851, Length=294, Percent_Identity=27.2108843537415, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=28.030303030303, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19921528, Length=289, Percent_Identity=27.681660899654, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66669; Mature: 66669

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRSHTCGELRPAHAGQEVTLAGWVHRRRDHGDLIFIDLRDRYGITQVVFNSAHPTAHEV
CCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCHHHH
AETVRSEYVLQVRGKVRIRPPEAVNPDLATGEIELEASEAQVLNPARTPPIYIAKEGGED
HHHHHHHEEEEECCEEEECCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEECCCCC
ESVRLKYRYLDLRRERMQRNLILRHRVVKFIRDFLDAEGFLEIETPILIKSTPEGARDYL
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCHHHCC
VPSRLHPGKFYALPQSPQQLKQLLMVAGYDKYFQIARCFRDEDQRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCCEEECCEEE
FVDQDDVLDLIERMFTALCRTVVPHKHLPTPFRRLSYAEAMERYGSDKPDLRYGLELVNL
ECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEECH
SDLLIETPFQVFRNVLNSGGQVKGIRAPGCAHFSRKQIDELTDVVRAGGAKGLAWAVLPT
HHHHHHCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
DGGEVRSSFAKNLAPGEFAAIVERMQAEAGDLLLIVADQPAVVAASLDKLRRNLAERLQL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
ADPNTLCFAWIVDFPLVEWNEDEQRWDAVHHPFTAPKDEDMHLLATDPGKVRAKAYDLIL
CCCCCEEEEEEHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHEEEEE
NGYEAGGGSIRIHRRDVQQQIFSLLGISEEQAQAQFGHMLEAFEYGAPPHGGIAPGIDRL
ECEECCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHE
VMILADEPTIREVMAFPKTQQAVDLMTNAPSPVDERQLKELHIRIVMPE
EEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHEEEEEEECC
>Mature Secondary Structure
MYRSHTCGELRPAHAGQEVTLAGWVHRRRDHGDLIFIDLRDRYGITQVVFNSAHPTAHEV
CCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCHHHH
AETVRSEYVLQVRGKVRIRPPEAVNPDLATGEIELEASEAQVLNPARTPPIYIAKEGGED
HHHHHHHEEEEECCEEEECCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEECCCCC
ESVRLKYRYLDLRRERMQRNLILRHRVVKFIRDFLDAEGFLEIETPILIKSTPEGARDYL
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCHHHCC
VPSRLHPGKFYALPQSPQQLKQLLMVAGYDKYFQIARCFRDEDQRADRQPEFTQLDMEMS
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCCEEECCEEE
FVDQDDVLDLIERMFTALCRTVVPHKHLPTPFRRLSYAEAMERYGSDKPDLRYGLELVNL
ECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEECH
SDLLIETPFQVFRNVLNSGGQVKGIRAPGCAHFSRKQIDELTDVVRAGGAKGLAWAVLPT
HHHHHHCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
DGGEVRSSFAKNLAPGEFAAIVERMQAEAGDLLLIVADQPAVVAASLDKLRRNLAERLQL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
ADPNTLCFAWIVDFPLVEWNEDEQRWDAVHHPFTAPKDEDMHLLATDPGKVRAKAYDLIL
CCCCCEEEEEEHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHEEEEE
NGYEAGGGSIRIHRRDVQQQIFSLLGISEEQAQAQFGHMLEAFEYGAPPHGGIAPGIDRL
ECEECCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHE
VMILADEPTIREVMAFPKTQQAVDLMTNAPSPVDERQLKELHIRIVMPE
EEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA