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The map label for this gene is hyuA [H]

Identifier: 222525686

GI number: 222525686

Start: 3076407

End: 3078452

Strand: Reverse

Name: hyuA [H]

Synonym: Chy400_2437

Alternate gene names: 222525686

Gene position: 3078452-3076407 (Counterclockwise)

Preceding gene: 222525687

Following gene: 222525685

Centisome position: 58.43

GC content: 59.43

Gene sequence:

>2046_bases
ATGAGTTATCGCATTGGAGTCGATGTTGGTGGAACGTTCACCGACCTGATGCTCTTCGATGAGGAGAGCGGACGCTACTG
GCGCTACAAGACCCCATCCACACCGCACGATCCGTCAGAGGCGGTGCTGTCCGGGATCGCGGCGATTTGCCAGCAAGCCG
GGATTGCTCCGTCGCAGGTAGCGCAGATCATGCACGGCACAACCGTCGCGACCAATGTTATTCTCGAAGGCAAAGGCGCA
CGAGTTGGACTGATCACTACCGAAGGGTTTAAGCAAATCCTGCATCTTGCCCGCTCACAAACGCCCGGCCCACTTGCCGG
CTGGATGATTATGATCAAGCCCGAACCACCGGCAACCCTTGCCGACACCCGCGAGGTACGCGAACGCATGAGTGCCCGTG
GCGAGGTGGTTCGCCCCCTTGACGAAGCCCAGGCCGAAGAGGTGATCCGCGAACTGGTCGCTTCCGGTATCGAGTCACTG
ACCATCTCGCTGATCAATTCGTATGCCAACCCCGACCACGAGCGTCGCCTGAAAGCGATTGCGCAACGCATTGCCCCCCA
GCTCCCGGTTACCATCTCTTACGATGTACTGCCGGAGTTTCGCGAGTACGAGCGCACGCTTACCGCGACCATGAATGCGT
ATGTCAAACCCAAGGTCAAAACCTACGTCCACAATCTCGATCAGCACCTGCGCCAACAGGGCGTGCAGGCGAAACTCAAT
ATTCTGCGCTCCGATGCCGGTTTGATGACTGCCGAAACGACCGAGGAGAATCCGGTCTATACCCTTCTCTCCGGTCCTTC
CGGTGGTGTAGCCGGAGCGTTGCATATCGCGTTGCGCGCCGGGTATCCCAATATTCTGACCTTCGATATGGGTGGTACGT
CAACCGACGTTTCGCTCTGTATGGGGGAACCCAATATCGCCCGCGAGACGGTGCTCGGCTACTTTCCGGTAAAAGTTCCT
TCGGTTGATGTGCGCTCGGTTGGTGCCGGTGGCGGTTCGATTGCTCACGTGCCTGCCCTGACGCGAGCGTTGCGGGTTGG
ACCACAGAGCGCCGGAGCGGTGCCCGGTCCGGCCTGCTACGGAAAAGGTGGTACCGAACCGACGGTGACCGATGCCAATC
TGGTCTGTGGGTATTTGCCACCGAGCATTCTCGGCGGGGCAATGCAGCTCGATGTCGCCGCAGCCAGGGCTGCCGTTCAG
AAGATCGCCGATGCCACCGGGTTGAGTCTGATGGCCGCAGCCGAAGGGATTCTGCGCATCGTGAACGAAAATATGTTCGG
TGCCCTCCGCCTGGTCAGTGTGCAGCGCGGGTTTGATCCGCGCCAGTTTGCGCTGATGGCCTTCGGTGGCGCCGGGCCAC
TCCACGGCAACGCCCTGGCCGAACTCCTCGGTTGTTATCCGGTGATTGTGCCACCCGCACCCGGTCTGCTCTGCGCCCTC
GGAGACCTGAGCGCCGATTACCGTGATGAGTTTGCCCGCACCTACATCCGCACCGTTGATAGGGTTGACCAGGAAGAGAT
GGTCGGTATGCTGCGCGATCTGGGGCGTGAAGCGCAGGCAATGCTGAGTGAAGAGGCGATCCCGCCATCCCGCCAGGAGA
TTCGCTACTTCGTTGATATGCGCTACTACCGACAGGGCTACGAGATGCCGATTGCCGTCAGCGAAGCCGAATTCGATCAA
GACCTGATCCCGATCCTGGTGCAACGCTTCAACGAACTGCACGACCGCACCTACGGTTTTATGCTCGACAGCAACGTGGA
AATTGTGAACCTGCGCGCAGTCGGGATTGGGCGCGTGACGAAAGTTGAGCTGCCCGAAGCCGAACCGGGTGACCCAGATG
CCAGCAGTGCTTACAGCGGCGATCATCAAATCTACCGCCACGGTGAATGGATCACGGCAAAACTCTACGACCGCAACAAG
CTGCGTCCGGGAATGCAGGTCGAGGGGCCGGCGATTATTACCGAGGTTGACAGCACTACGGTGGTACTACCCGGCCATAC
GGCAACCGTTGATCGCTACTTCAACCTTCTGATCAACCGCAGTTAA

Upstream 100 bases:

>100_bases
GAAGGCCGGGCCGGTCTATCCGCAGGTGGTGTCAATGGCACCCTACCTGTCATAAGCTACTGTCCGGGGAGTACGTGGTT
TTATCGGGAGGAATGGAGAC

Downstream 100 bases:

>100_bases
ACTGCTACAGCGAGGAGAATCTATGGCGACCAATCTCAAAATTGCGACGATCCCCCAGATCGAGATCGATCCGGTGACGC
TCGATATTATCGAGAATGCG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSYRIGVDVGGTFTDLMLFDEESGRYWRYKTPSTPHDPSEAVLSGIAAICQQAGIAPSQVAQIMHGTTVATNVILEGKGA
RVGLITTEGFKQILHLARSQTPGPLAGWMIMIKPEPPATLADTREVRERMSARGEVVRPLDEAQAEEVIRELVASGIESL
TISLINSYANPDHERRLKAIAQRIAPQLPVTISYDVLPEFREYERTLTATMNAYVKPKVKTYVHNLDQHLRQQGVQAKLN
ILRSDAGLMTAETTEENPVYTLLSGPSGGVAGALHIALRAGYPNILTFDMGGTSTDVSLCMGEPNIARETVLGYFPVKVP
SVDVRSVGAGGGSIAHVPALTRALRVGPQSAGAVPGPACYGKGGTEPTVTDANLVCGYLPPSILGGAMQLDVAAARAAVQ
KIADATGLSLMAAAEGILRIVNENMFGALRLVSVQRGFDPRQFALMAFGGAGPLHGNALAELLGCYPVIVPPAPGLLCAL
GDLSADYRDEFARTYIRTVDRVDQEEMVGMLRDLGREAQAMLSEEAIPPSRQEIRYFVDMRYYRQGYEMPIAVSEAEFDQ
DLIPILVQRFNELHDRTYGFMLDSNVEIVNLRAVGIGRVTKVELPEAEPGDPDASSAYSGDHQIYRHGEWITAKLYDRNK
LRPGMQVEGPAIITEVDSTTVVLPGHTATVDRYFNLLINRS

Sequences:

>Translated_681_residues
MSYRIGVDVGGTFTDLMLFDEESGRYWRYKTPSTPHDPSEAVLSGIAAICQQAGIAPSQVAQIMHGTTVATNVILEGKGA
RVGLITTEGFKQILHLARSQTPGPLAGWMIMIKPEPPATLADTREVRERMSARGEVVRPLDEAQAEEVIRELVASGIESL
TISLINSYANPDHERRLKAIAQRIAPQLPVTISYDVLPEFREYERTLTATMNAYVKPKVKTYVHNLDQHLRQQGVQAKLN
ILRSDAGLMTAETTEENPVYTLLSGPSGGVAGALHIALRAGYPNILTFDMGGTSTDVSLCMGEPNIARETVLGYFPVKVP
SVDVRSVGAGGGSIAHVPALTRALRVGPQSAGAVPGPACYGKGGTEPTVTDANLVCGYLPPSILGGAMQLDVAAARAAVQ
KIADATGLSLMAAAEGILRIVNENMFGALRLVSVQRGFDPRQFALMAFGGAGPLHGNALAELLGCYPVIVPPAPGLLCAL
GDLSADYRDEFARTYIRTVDRVDQEEMVGMLRDLGREAQAMLSEEAIPPSRQEIRYFVDMRYYRQGYEMPIAVSEAEFDQ
DLIPILVQRFNELHDRTYGFMLDSNVEIVNLRAVGIGRVTKVELPEAEPGDPDASSAYSGDHQIYRHGEWITAKLYDRNK
LRPGMQVEGPAIITEVDSTTVVLPGHTATVDRYFNLLINRS
>Mature_680_residues
SYRIGVDVGGTFTDLMLFDEESGRYWRYKTPSTPHDPSEAVLSGIAAICQQAGIAPSQVAQIMHGTTVATNVILEGKGAR
VGLITTEGFKQILHLARSQTPGPLAGWMIMIKPEPPATLADTREVRERMSARGEVVRPLDEAQAEEVIRELVASGIESLT
ISLINSYANPDHERRLKAIAQRIAPQLPVTISYDVLPEFREYERTLTATMNAYVKPKVKTYVHNLDQHLRQQGVQAKLNI
LRSDAGLMTAETTEENPVYTLLSGPSGGVAGALHIALRAGYPNILTFDMGGTSTDVSLCMGEPNIARETVLGYFPVKVPS
VDVRSVGAGGGSIAHVPALTRALRVGPQSAGAVPGPACYGKGGTEPTVTDANLVCGYLPPSILGGAMQLDVAAARAAVQK
IADATGLSLMAAAEGILRIVNENMFGALRLVSVQRGFDPRQFALMAFGGAGPLHGNALAELLGCYPVIVPPAPGLLCALG
DLSADYRDEFARTYIRTVDRVDQEEMVGMLRDLGREAQAMLSEEAIPPSRQEIRYFVDMRYYRQGYEMPIAVSEAEFDQD
LIPILVQRFNELHDRTYGFMLDSNVEIVNLRAVGIGRVTKVELPEAEPGDPDASSAYSGDHQIYRHGEWITAKLYDRNKL
RPGMQVEGPAIITEVDSTTVVLPGHTATVDRYFNLLINRS

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=741, Percent_Identity=30.7692307692308, Blast_Score=232, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI133901900, Length=704, Percent_Identity=25.8522727272727, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6322634, Length=741, Percent_Identity=28.0701754385965, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45550492, Length=734, Percent_Identity=29.4277929155313, Blast_Score=238, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73812; Mature: 73681

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRIGVDVGGTFTDLMLFDEESGRYWRYKTPSTPHDPSEAVLSGIAAICQQAGIAPSQV
CCEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
AQIMHGTTVATNVILEGKGARVGLITTEGFKQILHLARSQTPGPLAGWMIMIKPEPPATL
HHHHCCCHHEEEEEEECCCCEEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
ADTREVRERMSARGEVVRPLDEAQAEEVIRELVASGIESLTISLINSYANPDHERRLKAI
HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQRIAPQLPVTISYDVLPEFREYERTLTATMNAYVKPKVKTYVHNLDQHLRQQGVQAKLN
HHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
ILRSDAGLMTAETTEENPVYTLLSGPSGGVAGALHIALRAGYPNILTFDMGGTSTDVSLC
HHHCCCCCEEECCCCCCCEEEEEECCCCCHHHEEEEEEECCCCCEEEEECCCCCCCEEEE
MGEPNIARETVLGYFPVKVPSVDVRSVGAGGGSIAHVPALTRALRVGPQSAGAVPGPACY
ECCCCCCHHHHEEEECEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
GKGGTEPTVTDANLVCGYLPPSILGGAMQLDVAAARAAVQKIADATGLSLMAAAEGILRI
CCCCCCCCEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VNENMFGALRLVSVQRGFDPRQFALMAFGGAGPLHGNALAELLGCYPVIVPPAPGLLCAL
HHCCHHHHHEEEHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCEEEC
GDLSADYRDEFARTYIRTVDRVDQEEMVGMLRDLGREAQAMLSEEAIPPSRQEIRYFVDM
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHH
RYYRQGYEMPIAVSEAEFDQDLIPILVQRFNELHDRTYGFMLDSNVEIVNLRAVGIGRVT
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCEE
KVELPEAEPGDPDASSAYSGDHQIYRHGEWITAKLYDRNKLRPGMQVEGPAIITEVDSTT
EEECCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEECCCEEEEECCCCE
VVLPGHTATVDRYFNLLINRS
EEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYRIGVDVGGTFTDLMLFDEESGRYWRYKTPSTPHDPSEAVLSGIAAICQQAGIAPSQV
CEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
AQIMHGTTVATNVILEGKGARVGLITTEGFKQILHLARSQTPGPLAGWMIMIKPEPPATL
HHHHCCCHHEEEEEEECCCCEEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
ADTREVRERMSARGEVVRPLDEAQAEEVIRELVASGIESLTISLINSYANPDHERRLKAI
HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AQRIAPQLPVTISYDVLPEFREYERTLTATMNAYVKPKVKTYVHNLDQHLRQQGVQAKLN
HHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHH
ILRSDAGLMTAETTEENPVYTLLSGPSGGVAGALHIALRAGYPNILTFDMGGTSTDVSLC
HHHCCCCCEEECCCCCCCEEEEEECCCCCHHHEEEEEEECCCCCEEEEECCCCCCCEEEE
MGEPNIARETVLGYFPVKVPSVDVRSVGAGGGSIAHVPALTRALRVGPQSAGAVPGPACY
ECCCCCCHHHHEEEECEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
GKGGTEPTVTDANLVCGYLPPSILGGAMQLDVAAARAAVQKIADATGLSLMAAAEGILRI
CCCCCCCCEECCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VNENMFGALRLVSVQRGFDPRQFALMAFGGAGPLHGNALAELLGCYPVIVPPAPGLLCAL
HHCCHHHHHEEEHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCEEEC
GDLSADYRDEFARTYIRTVDRVDQEEMVGMLRDLGREAQAMLSEEAIPPSRQEIRYFVDM
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHH
RYYRQGYEMPIAVSEAEFDQDLIPILVQRFNELHDRTYGFMLDSNVEIVNLRAVGIGRVT
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCEE
KVELPEAEPGDPDASSAYSGDHQIYRHGEWITAKLYDRNKLRPGMQVEGPAIITEVDSTT
EEECCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCEECCCEEEEECCCCE
VVLPGHTATVDRYFNLLINRS
EEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]