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The map label for this gene is hyuB [H]

Identifier: 222525685

GI number: 222525685

Start: 3074450

End: 3076384

Strand: Reverse

Name: hyuB [H]

Synonym: Chy400_2436

Alternate gene names: 222525685

Gene position: 3076384-3074450 (Counterclockwise)

Preceding gene: 222525686

Following gene: 222525684

Centisome position: 58.39

GC content: 59.64

Gene sequence:

>1935_bases
ATGGCGACCAATCTCAAAATTGCGACGATCCCCCAGATCGAGATCGATCCGGTGACGCTCGATATTATCGAGAATGCGCT
CAAGAATACGCGCTATGAGATGGATGCTGTGCTTTACCGCACAGCAATGAGTCCGGTGATCCGCGAACAGCACGATCAGT
TTCCGATGATCACCGATCCGCACGGGCGGATGGTCGTTGGTCAATTCGGCTCGTACATCGCCGGCCTGCTCGACAACTGG
GATCAGACCATCGAACCGGGCGATGTTATTCTGCTCTCCGATCCATACCTCTGTGGTGGTGCCATCAGCCATATCAACGA
TTTGCTGGTGATGCTGCCCATCTTCTACGGCGAAGGCGATGAGCGGGAACTGGTCGGCTGGGCAAGCATGTTCGGCCATG
CCCAGGATGTCGGCGGGCCATTGCCCGGCTCACTGCCGACTACGGCGACGACCATCTTCGGCGAAGGTTTGCGGCTGCCG
CCGGTCAAAATCTACGAGCGCGGGAAGCTCAACCGGGCAGTCCTCGACATTATGCTCAACAACGTTCGCCAGCCTGAAAT
GAATCGCGCCGACCTGATGGCGATTATTGCCAGTTGCCGCACCGCCGAGAAGCGCGTGATCGAGCTGTGCAATCGCTTCG
GTAAAGATGTCTATCTGGCGGCTACGCAGGCGCTCCTCGACCGCACCTACCGGGCGATGAAGGAACTGATCGTGCGCAAC
CTGCCCGAAGAGCCACAGTCGTTTGAAGATTACGTTGACGACGACGGTCTCGGTAATGGGCCGTTCAAGATGAAGCTAAC
CATCTGGCGTGAAGGCCACGAAGCCTACTTCGACTGGACAGGCACCGATCCCCAGGCGATGGGGCCGATCAACTTCTACC
TCAACGAGTCGATGTTCAAGATGTTCATCGGCGTCTACCTGATCATGGTCTTCGACCCTCAAATCCTGTTCAACGACGGC
TTCTACCCGCTGCTGCACGTCACCATGCCGAAGGGCAGCCTGATCCAGCCCGAATTCCCGGCAGCACTCGGCTGTCGTAC
CCACGCCTTGACACGCCTGTTCGATGTGCTCGGCGGTGCGCTGGCCAAGCGAGCGCCTGAATTCACAACCGCCGCCGGTT
ATGGCACCTCACCCTACCTGCTCTACTCAGGCTATGATAAGGATGGCGAGTTCTTCTATCTGATGGAGATCAACTACGGT
GGGATTCCCGGTCGCCCAATTGGCGACGGTATGGACGGACACTCGTGGTGGCCGTTGTTCACCAACATCCCCACCGAATA
TCTGGAGAGCTACTACCCGATCCGTATCGAGCGTTACACCAGCATCATGGACTCAGGCGGTGCCGGTTTCCATCGCGGAG
GAAACGGGATCGAGAAGATTTACACCTTCCTTGAACCGGGTGAGATTTCGATCCACGATGACCGCTGGCTGACGCCACCG
TGGGGCAACGTCGGCGGCAAGCCGGGGAGTCGTTCGAGCAAGCTGCTGATCCGCACCGACGGCACCCGCCAGGTCTTGCC
CAGCAAGTGCGACCAGATCGCGGTCGAGCCGGGAGATCAACTGGTGTATCGGACTGCCGGTGGTGGCGGCTGGAAAGACC
CGCTCACCCGGCCACCAGAGGCAGTACAGCGCGACGTGCGTTACGGATTGGTAAGCCGCGAAAAGGCGCTGAACGATTAC
GGCGTCGTCCTCCGCGACGATCTCACGATTGACGAGGCAGCTACCGAAGCCAAACGCGCCGAACTGGCAGCAGCACGCGG
CGAAATTAAAGGCTTTGACTTCGGGCCACCGCTGGAAGAATTGCTGGCCAATGCCGAAGCCGAAACCGGTCTCCCGGCAC
CGCGCAAGCCGCAACCGGTACGCTGGGCCATGGCTCGCGCCGCTCGCCGCGAGCGAATGGCGTCGGCGCAGCAGCAGGCT
GTAACTGCCGACTGA

Upstream 100 bases:

>100_bases
GACAGCACTACGGTGGTACTACCCGGCCATACGGCAACCGTTGATCGCTACTTCAACCTTCTGATCAACCGCAGTTAAAC
TGCTACAGCGAGGAGAATCT

Downstream 100 bases:

>100_bases
TAGTTGCCAGAATTATGGGGGTGACAGGATATCCACCAGGGGATGGTACCATCCCGCTGCCTGTCACCCCAGCCTCTGCT
GTTCGGCAGCATAACTAGAG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MATNLKIATIPQIEIDPVTLDIIENALKNTRYEMDAVLYRTAMSPVIREQHDQFPMITDPHGRMVVGQFGSYIAGLLDNW
DQTIEPGDVILLSDPYLCGGAISHINDLLVMLPIFYGEGDERELVGWASMFGHAQDVGGPLPGSLPTTATTIFGEGLRLP
PVKIYERGKLNRAVLDIMLNNVRQPEMNRADLMAIIASCRTAEKRVIELCNRFGKDVYLAATQALLDRTYRAMKELIVRN
LPEEPQSFEDYVDDDGLGNGPFKMKLTIWREGHEAYFDWTGTDPQAMGPINFYLNESMFKMFIGVYLIMVFDPQILFNDG
FYPLLHVTMPKGSLIQPEFPAALGCRTHALTRLFDVLGGALAKRAPEFTTAAGYGTSPYLLYSGYDKDGEFFYLMEINYG
GIPGRPIGDGMDGHSWWPLFTNIPTEYLESYYPIRIERYTSIMDSGGAGFHRGGNGIEKIYTFLEPGEISIHDDRWLTPP
WGNVGGKPGSRSSKLLIRTDGTRQVLPSKCDQIAVEPGDQLVYRTAGGGGWKDPLTRPPEAVQRDVRYGLVSREKALNDY
GVVLRDDLTIDEAATEAKRAELAAARGEIKGFDFGPPLEELLANAEAETGLPAPRKPQPVRWAMARAARRERMASAQQQA
VTAD

Sequences:

>Translated_644_residues
MATNLKIATIPQIEIDPVTLDIIENALKNTRYEMDAVLYRTAMSPVIREQHDQFPMITDPHGRMVVGQFGSYIAGLLDNW
DQTIEPGDVILLSDPYLCGGAISHINDLLVMLPIFYGEGDERELVGWASMFGHAQDVGGPLPGSLPTTATTIFGEGLRLP
PVKIYERGKLNRAVLDIMLNNVRQPEMNRADLMAIIASCRTAEKRVIELCNRFGKDVYLAATQALLDRTYRAMKELIVRN
LPEEPQSFEDYVDDDGLGNGPFKMKLTIWREGHEAYFDWTGTDPQAMGPINFYLNESMFKMFIGVYLIMVFDPQILFNDG
FYPLLHVTMPKGSLIQPEFPAALGCRTHALTRLFDVLGGALAKRAPEFTTAAGYGTSPYLLYSGYDKDGEFFYLMEINYG
GIPGRPIGDGMDGHSWWPLFTNIPTEYLESYYPIRIERYTSIMDSGGAGFHRGGNGIEKIYTFLEPGEISIHDDRWLTPP
WGNVGGKPGSRSSKLLIRTDGTRQVLPSKCDQIAVEPGDQLVYRTAGGGGWKDPLTRPPEAVQRDVRYGLVSREKALNDY
GVVLRDDLTIDEAATEAKRAELAAARGEIKGFDFGPPLEELLANAEAETGLPAPRKPQPVRWAMARAARRERMASAQQQA
VTAD
>Mature_643_residues
ATNLKIATIPQIEIDPVTLDIIENALKNTRYEMDAVLYRTAMSPVIREQHDQFPMITDPHGRMVVGQFGSYIAGLLDNWD
QTIEPGDVILLSDPYLCGGAISHINDLLVMLPIFYGEGDERELVGWASMFGHAQDVGGPLPGSLPTTATTIFGEGLRLPP
VKIYERGKLNRAVLDIMLNNVRQPEMNRADLMAIIASCRTAEKRVIELCNRFGKDVYLAATQALLDRTYRAMKELIVRNL
PEEPQSFEDYVDDDGLGNGPFKMKLTIWREGHEAYFDWTGTDPQAMGPINFYLNESMFKMFIGVYLIMVFDPQILFNDGF
YPLLHVTMPKGSLIQPEFPAALGCRTHALTRLFDVLGGALAKRAPEFTTAAGYGTSPYLLYSGYDKDGEFFYLMEINYGG
IPGRPIGDGMDGHSWWPLFTNIPTEYLESYYPIRIERYTSIMDSGGAGFHRGGNGIEKIYTFLEPGEISIHDDRWLTPPW
GNVGGKPGSRSSKLLIRTDGTRQVLPSKCDQIAVEPGDQLVYRTAGGGGWKDPLTRPPEAVQRDVRYGLVSREKALNDYG
VVLRDDLTIDEAATEAKRAELAAARGEIKGFDFGPPLEELLANAEAETGLPAPRKPQPVRWAMARAARRERMASAQQQAV
TAD

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=549, Percent_Identity=29.143897996357, Blast_Score=152, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI133901900, Length=550, Percent_Identity=26.5454545454545, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133901902, Length=472, Percent_Identity=26.9067796610169, Blast_Score=115, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322634, Length=565, Percent_Identity=25.8407079646018, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45550492, Length=575, Percent_Identity=26.9565217391304, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 71650; Mature: 71518

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNLKIATIPQIEIDPVTLDIIENALKNTRYEMDAVLYRTAMSPVIREQHDQFPMITDP
CCCCCEEEECCCEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
HGRMVVGQFGSYIAGLLDNWDQTIEPGDVILLSDPYLCGGAISHINDLLVMLPIFYGEGD
CCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
ERELVGWASMFGHAQDVGGPLPGSLPTTATTIFGEGLRLPPVKIYERGKLNRAVLDIMLN
CHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHH
NVRQPEMNRADLMAIIASCRTAEKRVIELCNRFGKDVYLAATQALLDRTYRAMKELIVRN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHC
LPEEPQSFEDYVDDDGLGNGPFKMKLTIWREGHEAYFDWTGTDPQAMGPINFYLNESMFK
CCCCCHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCEEHHHHHHH
MFIGVYLIMVFDPQILFNDGFYPLLHVTMPKGSLIQPEFPAALGCRTHALTRLFDVLGGA
HHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAKRAPEFTTAAGYGTSPYLLYSGYDKDGEFFYLMEINYGGIPGRPIGDGMDGHSWWPLF
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
TNIPTEYLESYYPIRIERYTSIMDSGGAGFHRGGNGIEKIYTFLEPGEISIHDDRWLTPP
HCCCHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCC
WGNVGGKPGSRSSKLLIRTDGTRQVLPSKCDQIAVEPGDQLVYRTAGGGGWKDPLTRPPE
CCCCCCCCCCCCCEEEEEECCCHHHCCHHCCEEEECCCCEEEEEECCCCCCCCCCCCCHH
AVQRDVRYGLVSREKALNDYGVVLRDDLTIDEAATEAKRAELAAARGEIKGFDFGPPLEE
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LLANAEAETGLPAPRKPQPVRWAMARAARRERMASAQQQAVTAD
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATNLKIATIPQIEIDPVTLDIIENALKNTRYEMDAVLYRTAMSPVIREQHDQFPMITDP
CCCCEEEECCCEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
HGRMVVGQFGSYIAGLLDNWDQTIEPGDVILLSDPYLCGGAISHINDLLVMLPIFYGEGD
CCCEEHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
ERELVGWASMFGHAQDVGGPLPGSLPTTATTIFGEGLRLPPVKIYERGKLNRAVLDIMLN
CHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHH
NVRQPEMNRADLMAIIASCRTAEKRVIELCNRFGKDVYLAATQALLDRTYRAMKELIVRN
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHC
LPEEPQSFEDYVDDDGLGNGPFKMKLTIWREGHEAYFDWTGTDPQAMGPINFYLNESMFK
CCCCCHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCEEHHHHHHH
MFIGVYLIMVFDPQILFNDGFYPLLHVTMPKGSLIQPEFPAALGCRTHALTRLFDVLGGA
HHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAKRAPEFTTAAGYGTSPYLLYSGYDKDGEFFYLMEINYGGIPGRPIGDGMDGHSWWPLF
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
TNIPTEYLESYYPIRIERYTSIMDSGGAGFHRGGNGIEKIYTFLEPGEISIHDDRWLTPP
HCCCHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCC
WGNVGGKPGSRSSKLLIRTDGTRQVLPSKCDQIAVEPGDQLVYRTAGGGGWKDPLTRPPE
CCCCCCCCCCCCCEEEEEECCCHHHCCHHCCEEEECCCCEEEEEECCCCCCCCCCCCCHH
AVQRDVRYGLVSREKALNDYGVVLRDDLTIDEAATEAKRAELAAARGEIKGFDFGPPLEE
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LLANAEAETGLPAPRKPQPVRWAMARAARRERMASAQQQAVTAD
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]