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The map label for this gene is mrpA [H]

Identifier: 222525634

GI number: 222525634

Start: 3012463

End: 3014835

Strand: Direct

Name: mrpA [H]

Synonym: Chy400_2385

Alternate gene names: 222525634

Gene position: 3012463-3014835 (Clockwise)

Preceding gene: 222525632

Following gene: 222525635

Centisome position: 57.17

GC content: 57.06

Gene sequence:

>2373_bases
ATGATCTATCCGCTTGTATTCATTGCGCTAGCGGCAGCTCCCCTGGCATTACTCGCCCGCTTCCCGGCGCGTGACCGAAT
TGCGTGGGGGTTGGCGTTGATCCCGTTGCTCATCTTCGGTCTGACGCTGGCGCAGGCTGCGCCGGTGTGGGAAGGCGGGC
TGGTGACTGAACGGATAGAGTGGGTGCCGGCGCTAGGGTTGCATCTGAGCTTTACGCTTGATGGTTTGAGCCTGCTGTTT
GCCCTCATTATCTCTGGCATTGGGACATTGATCGTCGGTTATGCCGGCCACTATCTCGGATCTGACAGCGGTCTTGGCCG
TTTTCTGGCTTACCTGATGCTGTTTATGGGTGCGATGCTGGGGCTGGTGCTGGCCGGCAATGTGCTGACGATGTTTGTGT
TCTGGGAATTGACCTCGGTAACGTCGTATTTGCTCATTGGCTACAAGCACGATTATGCCGATGCCCGGCGGGGCGCCCTA
CATTCACTCCTGGTAACCGGTGGCGGTGGACTGGCGCTGCTCGTCGGCTTGTTACTGCTTGGTCAGGCGGTTGGGAGCTA
CGAGTTCAGTGATATTCTGGCGGCTGGCGATACGTTGCACGCGCATCCGCTTTACCCGGCAGCAGTGATCTTAATTCTGC
TTGGCTGTTTCACCAAATCGGCCCAGATACCCTTCCACTTCTGGCTTCCCGGTGCGATGCAGGCACCGACACCGGCCAGT
GCCTTTCTCCACTCGGCCACAATGGTCAAGGCCGGGGTATACCTCATGGCGCGACTCAGCCCGGCACTGGGTGGAACCGC
GCTGTGGGATGTGACGCTGGCAGTGGTTGGCGGGGCGACAATGACCTTCGCTGCGATTGTGGCGATGCGGCAGTTCGATC
TGAAGGCGATGCTGGCCTATACCACGATCAGCATGCTGGGTGCCTTGACGATGCAGATCGGATTGGGTGGGAAGTACGGT
GCCGAAGGTGTGGCGACAAATATTCTGGCCCACGCGCTGTACAAATCGGCCCTGTTTATGCTTGCCGGGGTGATTGACCA
CGAGTGTGGGACGCGCGATCTCCGTCGTCTCGGCGGACTGCGTAAGTACATGCCGCAGACGATGGTGATTACGATGCTGG
CTTTGCTTTCATTTGGTGGCATTCCGGTGATGTTTGGCTTTGTAGCCAAAGAGCTAATGCTCGAAGCAGCGCTGGAAACC
GAGTTGGGTGCGACATTAAGTGTGCTGGCGGTGGCAGCCATTGCCATCACTGCTATCGCCTATCTGATTACTGGCTGGCG
CCTGTTCAGCAATGCCTTCCTCGGTCAGCGCAGTGTTGCAGCGATGCAGCAGCATATTACCGATCCGGCACCGGGGATGT
TGATCGGTCCTGGTGTTCCGGCAGTATTGTCATTGATCATCCCTATCGCGTTTTTGGGACCGATCAGTACGTTGATCGCT
GCGGCCAGTGCATCTATTTATGGTCAGCCGCTTGACGTGAAACTGGCGCTCTGGCACGGGATTAATCCCGCACTGCTGGT
GAGTCTGAGCGCGATTGTGATTGGCGTCGTGATGTTCCGTTTCGTCGATCAACTGGCCGGTTTACGCGGTTGGCCGGCCT
GGTCACGCGGCGATATGATCTTCGACCGTCTGATCGAGAGTACCCTGACCGGTGCTACCGCGTTGACGCGAACCATTCAG
GAAGGCCGTCTGCGCCGATATATTCTGCTCACGGCGCTCACCATGCTGGTTTTCGTTGGTGTTCCGTTTGTCAGTTTTGG
ACTTGGGGCGATTCGCTTCGATCTCGATCCACAGTTGCAGCTCTACGAGATACTGGCGGCGGCTCTCATACCGATTGGAG
TTATTGCCACGATTAACGCTCGCACCCGTCTGGGAGCCATCATTGCTGTTGGTGTTGTCGGCGCAATGGTATCGCTCCTC
TTCGTTCTCTTCTCAGCGCCTGACCTGGCATTGACCCAGTTGCTGATTGAAGTGCTCTCAACCGTGTTTCTGTTACTGGT
CTTCTCCGTCCTACCGGTACGCTTCGAGAGCTTCTCGTCGGCCTGGGTGCGGCGGCGCGATGCGATTGTGGCGACGGTCA
TGGGTGTGCTGATGGGTGGTCTGGTACTGGCAGCGGCCACAAACGATAGTTTTGCGCCGTTAGCACCCTTCTTCCTGGAA
AATAGTCTGGAACTGGGCAAAGGCGCCAACGTCGTGAATGTAATCCTGGTTGACTTCCGCGGTTTCGATACCATGGGCGA
AATTACGGTCTTGTTCATCGCGCTCCTTGGTATCTATGGCTTGTTACGGTTACGACAGGGGAAAGACAATGCGACACCAG
AGCGGGTCACCACAACCGAACCGGTCAACGAACCGGTCGATGCCCAGAGATAG

Upstream 100 bases:

>100_bases
CAACTGGAACGTTGTCCAGCCGTTGCCACTTTCGCTGATGGTGTCGTACCGTGCCACGTCAGGTGGTAGTAGCGTTTTGT
AGTGTTTGAACGAGGAGAGC

Downstream 100 bases:

>100_bases
TGCCTTATACGACTCGTTAATTCTGCGCACGATTAGCCGGTTGATGATGCCGGTGCTGTTGTTGCTTTCAATCTTTATGC
TCCTGCGCGGCCATAATTTG

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 790; Mature: 790

Protein sequence:

>790_residues
MIYPLVFIALAAAPLALLARFPARDRIAWGLALIPLLIFGLTLAQAAPVWEGGLVTERIEWVPALGLHLSFTLDGLSLLF
ALIISGIGTLIVGYAGHYLGSDSGLGRFLAYLMLFMGAMLGLVLAGNVLTMFVFWELTSVTSYLLIGYKHDYADARRGAL
HSLLVTGGGGLALLVGLLLLGQAVGSYEFSDILAAGDTLHAHPLYPAAVILILLGCFTKSAQIPFHFWLPGAMQAPTPAS
AFLHSATMVKAGVYLMARLSPALGGTALWDVTLAVVGGATMTFAAIVAMRQFDLKAMLAYTTISMLGALTMQIGLGGKYG
AEGVATNILAHALYKSALFMLAGVIDHECGTRDLRRLGGLRKYMPQTMVITMLALLSFGGIPVMFGFVAKELMLEAALET
ELGATLSVLAVAAIAITAIAYLITGWRLFSNAFLGQRSVAAMQQHITDPAPGMLIGPGVPAVLSLIIPIAFLGPISTLIA
AASASIYGQPLDVKLALWHGINPALLVSLSAIVIGVVMFRFVDQLAGLRGWPAWSRGDMIFDRLIESTLTGATALTRTIQ
EGRLRRYILLTALTMLVFVGVPFVSFGLGAIRFDLDPQLQLYEILAAALIPIGVIATINARTRLGAIIAVGVVGAMVSLL
FVLFSAPDLALTQLLIEVLSTVFLLLVFSVLPVRFESFSSAWVRRRDAIVATVMGVLMGGLVLAAATNDSFAPLAPFFLE
NSLELGKGANVVNVILVDFRGFDTMGEITVLFIALLGIYGLLRLRQGKDNATPERVTTTEPVNEPVDAQR

Sequences:

>Translated_790_residues
MIYPLVFIALAAAPLALLARFPARDRIAWGLALIPLLIFGLTLAQAAPVWEGGLVTERIEWVPALGLHLSFTLDGLSLLF
ALIISGIGTLIVGYAGHYLGSDSGLGRFLAYLMLFMGAMLGLVLAGNVLTMFVFWELTSVTSYLLIGYKHDYADARRGAL
HSLLVTGGGGLALLVGLLLLGQAVGSYEFSDILAAGDTLHAHPLYPAAVILILLGCFTKSAQIPFHFWLPGAMQAPTPAS
AFLHSATMVKAGVYLMARLSPALGGTALWDVTLAVVGGATMTFAAIVAMRQFDLKAMLAYTTISMLGALTMQIGLGGKYG
AEGVATNILAHALYKSALFMLAGVIDHECGTRDLRRLGGLRKYMPQTMVITMLALLSFGGIPVMFGFVAKELMLEAALET
ELGATLSVLAVAAIAITAIAYLITGWRLFSNAFLGQRSVAAMQQHITDPAPGMLIGPGVPAVLSLIIPIAFLGPISTLIA
AASASIYGQPLDVKLALWHGINPALLVSLSAIVIGVVMFRFVDQLAGLRGWPAWSRGDMIFDRLIESTLTGATALTRTIQ
EGRLRRYILLTALTMLVFVGVPFVSFGLGAIRFDLDPQLQLYEILAAALIPIGVIATINARTRLGAIIAVGVVGAMVSLL
FVLFSAPDLALTQLLIEVLSTVFLLLVFSVLPVRFESFSSAWVRRRDAIVATVMGVLMGGLVLAAATNDSFAPLAPFFLE
NSLELGKGANVVNVILVDFRGFDTMGEITVLFIALLGIYGLLRLRQGKDNATPERVTTTEPVNEPVDAQR
>Mature_790_residues
MIYPLVFIALAAAPLALLARFPARDRIAWGLALIPLLIFGLTLAQAAPVWEGGLVTERIEWVPALGLHLSFTLDGLSLLF
ALIISGIGTLIVGYAGHYLGSDSGLGRFLAYLMLFMGAMLGLVLAGNVLTMFVFWELTSVTSYLLIGYKHDYADARRGAL
HSLLVTGGGGLALLVGLLLLGQAVGSYEFSDILAAGDTLHAHPLYPAAVILILLGCFTKSAQIPFHFWLPGAMQAPTPAS
AFLHSATMVKAGVYLMARLSPALGGTALWDVTLAVVGGATMTFAAIVAMRQFDLKAMLAYTTISMLGALTMQIGLGGKYG
AEGVATNILAHALYKSALFMLAGVIDHECGTRDLRRLGGLRKYMPQTMVITMLALLSFGGIPVMFGFVAKELMLEAALET
ELGATLSVLAVAAIAITAIAYLITGWRLFSNAFLGQRSVAAMQQHITDPAPGMLIGPGVPAVLSLIIPIAFLGPISTLIA
AASASIYGQPLDVKLALWHGINPALLVSLSAIVIGVVMFRFVDQLAGLRGWPAWSRGDMIFDRLIESTLTGATALTRTIQ
EGRLRRYILLTALTMLVFVGVPFVSFGLGAIRFDLDPQLQLYEILAAALIPIGVIATINARTRLGAIIAVGVVGAMVSLL
FVLFSAPDLALTQLLIEVLSTVFLLLVFSVLPVRFESFSSAWVRRRDAIVATVMGVLMGGLVLAAATNDSFAPLAPFFLE
NSLELGKGANVVNVILVDFRGFDTMGEITVLFIALLGIYGLLRLRQGKDNATPERVTTTEPVNEPVDAQR

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=361, Percent_Identity=33.5180055401662, Blast_Score=182, Evalue=8e-46,
Organism=Escherichia coli, GI1788614, Length=338, Percent_Identity=34.0236686390533, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788829, Length=395, Percent_Identity=32.4050632911392, Blast_Score=160, Evalue=4e-40,
Organism=Escherichia coli, GI1788613, Length=383, Percent_Identity=28.1984334203655, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788827, Length=314, Percent_Identity=27.7070063694268, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788831, Length=346, Percent_Identity=28.3236994219653, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI145693160, Length=302, Percent_Identity=26.4900662251656, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI2367154, Length=186, Percent_Identity=27.9569892473118, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 83969; Mature: 83969

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPLVFIALAAAPLALLARFPARDRIAWGLALIPLLIFGLTLAQAAPVWEGGLVTERIE
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WVPALGLHLSFTLDGLSLLFALIISGIGTLIVGYAGHYLGSDSGLGRFLAYLMLFMGAML
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GLVLAGNVLTMFVFWELTSVTSYLLIGYKHDYADARRGALHSLLVTGGGGLALLVGLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHH
GQAVGSYEFSDILAAGDTLHAHPLYPAAVILILLGCFTKSAQIPFHFWLPGAMQAPTPAS
HHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHH
AFLHSATMVKAGVYLMARLSPALGGTALWDVTLAVVGGATMTFAAIVAMRQFDLKAMLAY
HHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TTISMLGALTMQIGLGGKYGAEGVATNILAHALYKSALFMLAGVIDHECGTRDLRRLGGL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHH
RKYMPQTMVITMLALLSFGGIPVMFGFVAKELMLEAALETELGATLSVLAVAAIAITAIA
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
YLITGWRLFSNAFLGQRSVAAMQQHITDPAPGMLIGPGVPAVLSLIIPIAFLGPISTLIA
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
AASASIYGQPLDVKLALWHGINPALLVSLSAIVIGVVMFRFVDQLAGLRGWPAWSRGDMI
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FDRLIESTLTGATALTRTIQEGRLRRYILLTALTMLVFVGVPFVSFGLGAIRFDLDPQLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
LYEILAAALIPIGVIATINARTRLGAIIAVGVVGAMVSLLFVLFSAPDLALTQLLIEVLS
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVFLLLVFSVLPVRFESFSSAWVRRRDAIVATVMGVLMGGLVLAAATNDSFAPLAPFFLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
NSLELGKGANVVNVILVDFRGFDTMGEITVLFIALLGIYGLLRLRQGKDNATPERVTTTE
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVNEPVDAQR
CCCCCCCCCC
>Mature Secondary Structure
MIYPLVFIALAAAPLALLARFPARDRIAWGLALIPLLIFGLTLAQAAPVWEGGLVTERIE
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WVPALGLHLSFTLDGLSLLFALIISGIGTLIVGYAGHYLGSDSGLGRFLAYLMLFMGAML
HHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GLVLAGNVLTMFVFWELTSVTSYLLIGYKHDYADARRGALHSLLVTGGGGLALLVGLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHH
GQAVGSYEFSDILAAGDTLHAHPLYPAAVILILLGCFTKSAQIPFHFWLPGAMQAPTPAS
HHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHH
AFLHSATMVKAGVYLMARLSPALGGTALWDVTLAVVGGATMTFAAIVAMRQFDLKAMLAY
HHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TTISMLGALTMQIGLGGKYGAEGVATNILAHALYKSALFMLAGVIDHECGTRDLRRLGGL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHH
RKYMPQTMVITMLALLSFGGIPVMFGFVAKELMLEAALETELGATLSVLAVAAIAITAIA
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
YLITGWRLFSNAFLGQRSVAAMQQHITDPAPGMLIGPGVPAVLSLIIPIAFLGPISTLIA
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
AASASIYGQPLDVKLALWHGINPALLVSLSAIVIGVVMFRFVDQLAGLRGWPAWSRGDMI
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FDRLIESTLTGATALTRTIQEGRLRRYILLTALTMLVFVGVPFVSFGLGAIRFDLDPQLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
LYEILAAALIPIGVIATINARTRLGAIIAVGVVGAMVSLLFVLFSAPDLALTQLLIEVLS
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVFLLLVFSVLPVRFESFSSAWVRRRDAIVATVMGVLMGGLVLAAATNDSFAPLAPFFLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
NSLELGKGANVVNVILVDFRGFDTMGEITVLFIALLGIYGLLRLRQGKDNATPERVTTTE
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVNEPVDAQR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]