Click here to switch to the map view.

The map label for this gene is panB

Identifier: 222525629

GI number: 222525629

Start: 3007205

End: 3008050

Strand: Reverse

Name: panB

Synonym: Chy400_2380

Alternate gene names: 222525629

Gene position: 3008050-3007205 (Counterclockwise)

Preceding gene: 222525631

Following gene: 222525628

Centisome position: 57.09

GC content: 59.34

Gene sequence:

>846_bases
ATGCGCATAACCATTCGCGATATTCAGCAGATGCGCGATCGCGGCGAGCGAATCCCAATGGTCACCGCGTATGACTATAC
CTCGGCTCAGATTGCTGATCGCGCCGGAATTCCATTGATTCTGGTAGGGGATTCGTTAGGCATGGTCGTGCTGGGCTACA
ATTCTACCGTACCGGTGACGCTTGACGACATGATTCACCATACGCGCGCCGTCGTGCGTGGTACGCAAAAAGCCCTGGTT
ATCGGTGATCTCCCATTCCTTACCTACACATCACCAGAGCAGGCGATGCAGAGTGCCGGACGGATGTTGCAGGAAGCCGG
TGCGCAGGCCGTCAAGCTGGAAGGCGGTGTGCATATCGCTCCTACCATTGCCCGCCTGGTTCAGGCCGGGATTCCGGTGA
TGGGGCACATTGGCTTTACGCCACAGGCGGTTAACCAGATTGGGTTGCGCGTGCAAGGGCGACGGGCTGCTGAGGCGCAA
CGACTGCTCGCCGATGCGCTGGCCGTGCAAGAAGCCGGTGCCTTCGCGATTGTGCTGGAGCTGGTACCGGCAGAGCTGGC
CCAGGCCATTACGGAACGGTTGCGGATTCCGACTATCGGGATCGGTGCCGGAGCCGGCTGTAGCGGTCAGGTGCAGGTCT
GGCACGATATGCTCGGTCTGTACAGCGACTTTTTACCCCGTCACGCAAAGCGGTACGCCGATCTGGCAACAATTATTGCC
GAGGCGCTGAGTCAGTACGCCAGTGATGTGCGTAATGGTACGTTCCCTGGCCCTGAACATAGCAGTCGGATGGATCCGGC
AGAGCTAGCCGCAGCGCTGGGGAGTCAAGATCAGGCGACGGAATAG

Upstream 100 bases:

>100_bases
GGCATCTAGCGAAACAACCAGCTTTTTCCGTTGGTGTGCTACAATCGAGCAATCAAATAGCCGGAACGACCGGCAGAGCA
CAACTAATAAGGAGCCGGCA

Downstream 100 bases:

>100_bases
GCAACGATGACGAAGAACCTCTTCGGGCATCATCCCGCAGCGGTTTGCAAAGCTGCTCGTTGCGGCTTGGTGAGCGCGCC
GGATGTGTGCGCTCCCAGGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRITIRDIQQMRDRGERIPMVTAYDYTSAQIADRAGIPLILVGDSLGMVVLGYNSTVPVTLDDMIHHTRAVVRGTQKALV
IGDLPFLTYTSPEQAMQSAGRMLQEAGAQAVKLEGGVHIAPTIARLVQAGIPVMGHIGFTPQAVNQIGLRVQGRRAAEAQ
RLLADALAVQEAGAFAIVLELVPAELAQAITERLRIPTIGIGAGAGCSGQVQVWHDMLGLYSDFLPRHAKRYADLATIIA
EALSQYASDVRNGTFPGPEHSSRMDPAELAAALGSQDQATE

Sequences:

>Translated_281_residues
MRITIRDIQQMRDRGERIPMVTAYDYTSAQIADRAGIPLILVGDSLGMVVLGYNSTVPVTLDDMIHHTRAVVRGTQKALV
IGDLPFLTYTSPEQAMQSAGRMLQEAGAQAVKLEGGVHIAPTIARLVQAGIPVMGHIGFTPQAVNQIGLRVQGRRAAEAQ
RLLADALAVQEAGAFAIVLELVPAELAQAITERLRIPTIGIGAGAGCSGQVQVWHDMLGLYSDFLPRHAKRYADLATIIA
EALSQYASDVRNGTFPGPEHSSRMDPAELAAALGSQDQATE
>Mature_281_residues
MRITIRDIQQMRDRGERIPMVTAYDYTSAQIADRAGIPLILVGDSLGMVVLGYNSTVPVTLDDMIHHTRAVVRGTQKALV
IGDLPFLTYTSPEQAMQSAGRMLQEAGAQAVKLEGGVHIAPTIARLVQAGIPVMGHIGFTPQAVNQIGLRVQGRRAAEAQ
RLLADALAVQEAGAFAIVLELVPAELAQAITERLRIPTIGIGAGAGCSGQVQVWHDMLGLYSDFLPRHAKRYADLATIIA
EALSQYASDVRNGTFPGPEHSSRMDPAELAAALGSQDQATE

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=259, Percent_Identity=45.5598455598456, Blast_Score=227, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6319653, Length=262, Percent_Identity=37.7862595419847, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CHLAA (A9WFR7)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635806.1
- ProteinModelPortal:   A9WFR7
- SMR:   A9WFR7
- GeneID:   5826668
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2208
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30025; Mature: 30025

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITIRDIQQMRDRGERIPMVTAYDYTSAQIADRAGIPLILVGDSLGMVVLGYNSTVPVT
CEEEHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEE
LDDMIHHTRAVVRGTQKALVIGDLPFLTYTSPEQAMQSAGRMLQEAGAQAVKLEGGVHIA
HHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCEEEECCCCEEH
PTIARLVQAGIPVMGHIGFTPQAVNQIGLRVQGRRAAEAQRLLADALAVQEAGAFAIVLE
HHHHHHHHHCCCEEECCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHH
LVPAELAQAITERLRIPTIGIGAGAGCSGQVQVWHDMLGLYSDFLPRHAKRYADLATIIA
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALSQYASDVRNGTFPGPEHSSRMDPAELAAALGSQDQATE
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRITIRDIQQMRDRGERIPMVTAYDYTSAQIADRAGIPLILVGDSLGMVVLGYNSTVPVT
CEEEHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEE
LDDMIHHTRAVVRGTQKALVIGDLPFLTYTSPEQAMQSAGRMLQEAGAQAVKLEGGVHIA
HHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCEEEECCCCEEH
PTIARLVQAGIPVMGHIGFTPQAVNQIGLRVQGRRAAEAQRLLADALAVQEAGAFAIVLE
HHHHHHHHHCCCEEECCCCCHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHH
LVPAELAQAITERLRIPTIGIGAGAGCSGQVQVWHDMLGLYSDFLPRHAKRYADLATIIA
HHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALSQYASDVRNGTFPGPEHSSRMDPAELAAALGSQDQATE
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA