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The map label for this gene is puuR [H]

Identifier: 222525574

GI number: 222525574

Start: 2936131

End: 2936766

Strand: Reverse

Name: puuR [H]

Synonym: Chy400_2323

Alternate gene names: 222525574

Gene position: 2936766-2936131 (Counterclockwise)

Preceding gene: 222525575

Following gene: 222525573

Centisome position: 55.74

GC content: 57.55

Gene sequence:

>636_bases
ATGGTTGATCCAGTGAGTAGTCCGCATCTCGTTGGTGAAATTGGCAATGTAGACGTTGGTACACGCATTCGGACCCTGCG
TGAACAACGGCGCTTATCAATCCGCGCCCTCGCCGAGGCGAGCGGGCTTGCCGTCAATACCCTCAGTCTGATTGAAAATG
GTCGCACGTCACCGAGTGTCAGCACCTTGCAACGTCTTGCAGTGGCTCTACAGGTTCCGGTTAGTACCTTCTTTACGCCC
GATGTGCCACAGCAACGTATCGTCTTCACCCCGGCAGGTCAGGCCATCGCCAGCCTCTTCCCGCACGGTGCCATGGCCGA
TCTCGCCCCAGGCATGATCAACCGCACCCTCGAACCGCTGCTGGTCACGCTCGAACCCGGCGCCGGTAGTGGCCCTGAAG
CAATTGTCCATACCGGACAGGAATTTGTCTTTGGTTTAAATGGGCAGATTCAGTACACCGTTGCCGATCAGATGTTCATC
ATCAATCCGGGTGATAGCCTGCTGTTCGAGGCCGCTCTCCCCCACCGCTGGCATAACCCCGGCCATACGCCAGCCCAGGT
GCTGCTGGTCTTGTGTCCGCTTGAATTACCCCGGAGTGTGATTGCCCGTCATAGCCCTTATCAGGGAAGGAGTTGA

Upstream 100 bases:

>100_bases
TTACTGAAAGACAACCATTTTGTTGTGTGAAAATCAGAAAAATGTCTTATAATAATGAACAGATCAAGCTGCCGAGTTAG
AAAGATAGCCGGTGCCACCT

Downstream 100 bases:

>100_bases
AACGATGCCAATCCGTGCCTTACACCTGAGCGAAGATGGAGTCAGCCGCGCCGAACAAGACATCACGTTAACCCGGATTG
CGCCCGGTCAGATTACGCTC

Product: XRE family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MVDPVSSPHLVGEIGNVDVGTRIRTLREQRRLSIRALAEASGLAVNTLSLIENGRTSPSVSTLQRLAVALQVPVSTFFTP
DVPQQRIVFTPAGQAIASLFPHGAMADLAPGMINRTLEPLLVTLEPGAGSGPEAIVHTGQEFVFGLNGQIQYTVADQMFI
INPGDSLLFEAALPHRWHNPGHTPAQVLLVLCPLELPRSVIARHSPYQGRS

Sequences:

>Translated_211_residues
MVDPVSSPHLVGEIGNVDVGTRIRTLREQRRLSIRALAEASGLAVNTLSLIENGRTSPSVSTLQRLAVALQVPVSTFFTP
DVPQQRIVFTPAGQAIASLFPHGAMADLAPGMINRTLEPLLVTLEPGAGSGPEAIVHTGQEFVFGLNGQIQYTVADQMFI
INPGDSLLFEAALPHRWHNPGHTPAQVLLVLCPLELPRSVIARHSPYQGRS
>Mature_211_residues
MVDPVSSPHLVGEIGNVDVGTRIRTLREQRRLSIRALAEASGLAVNTLSLIENGRTSPSVSTLQRLAVALQVPVSTFFTP
DVPQQRIVFTPAGQAIASLFPHGAMADLAPGMINRTLEPLLVTLEPGAGSGPEAIVHTGQEFVFGLNGQIQYTVADQMFI
INPGDSLLFEAALPHRWHNPGHTPAQVLLVLCPLELPRSVIARHSPYQGRS

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=168, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1787704, Length=167, Percent_Identity=32.9341317365269, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 22657; Mature: 22657

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50943 HTH_CROC1 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPVSSPHLVGEIGNVDVGTRIRTLREQRRLSIRALAEASGLAVNTLSLIENGRTSPSV
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
STLQRLAVALQVPVSTFFTPDVPQQRIVFTPAGQAIASLFPHGAMADLAPGMINRTLEPL
HHHHHHHHHHHCCHHHCCCCCCCCCEEEECCCHHHHHHHCCCCCHHHHCCHHHHHCCCCE
LVTLEPGAGSGPEAIVHTGQEFVFGLNGQIQYTVADQMFIINPGDSLLFEAALPHRWHNP
EEEECCCCCCCCHHHHCCCCCEEEECCCEEEEEEECEEEEECCCCCEEEEECCCCCCCCC
GHTPAQVLLVLCPLELPRSVIARHSPYQGRS
CCCHHHHHHHHCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MVDPVSSPHLVGEIGNVDVGTRIRTLREQRRLSIRALAEASGLAVNTLSLIENGRTSPSV
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
STLQRLAVALQVPVSTFFTPDVPQQRIVFTPAGQAIASLFPHGAMADLAPGMINRTLEPL
HHHHHHHHHHHCCHHHCCCCCCCCCEEEECCCHHHHHHHCCCCCHHHHCCHHHHHCCCCE
LVTLEPGAGSGPEAIVHTGQEFVFGLNGQIQYTVADQMFIINPGDSLLFEAALPHRWHNP
EEEECCCCCCCCHHHHCCCCCEEEECCCEEEEEEECEEEEECCCCCEEEEECCCCCCCCC
GHTPAQVLLVLCPLELPRSVIARHSPYQGRS
CCCHHHHHHHHCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]