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The map label for this gene is wcaI [H]

Identifier: 222525545

GI number: 222525545

Start: 2900880

End: 2902115

Strand: Direct

Name: wcaI [H]

Synonym: Chy400_2294

Alternate gene names: 222525545

Gene position: 2900880-2902115 (Clockwise)

Preceding gene: 222525544

Following gene: 222525549

Centisome position: 55.06

GC content: 57.04

Gene sequence:

>1236_bases
ATGCATATCCTCTATCTCCATCAATACTTCACAACCCGCACCGGGGTAGGCGGTACACGTTCCTATGAGTTTGCGCGTTT
CTTCATTCAGCAAGGCCATCGGGTGACCATGGTTACTGCTGCCAACCGCCAGGAGCCATGGTCTGGCGGGTTGTGGCGAC
GGCGCGTGGTTGATGGGATCAACGTGGTTGAGGTACGGGCCGGTTATGCCGACTATAGCCGGGGCACGGCAATTAGCTAC
GGCCAACGCATTATCGCCTTTCTGCTCTTTGCTCTGGCCAGCCTGATCGCAGTGGTACGGGTGGAACGGCCTGATGTGGT
GTTTGCTACCAGCACTCCCTTGACGATTGGGATTCCCGGTATGCTGGCCAGCCGCTGGCATCGCGTGCCACTGGTCTTTG
AAGTACGCGATCTATGGCCAGAGGCACCAATCCAGATGGGTGTACTTCGGCATCCCCTCCTCATTCTGGCTGCACGCTGG
CTTGAACGAGCCATTTATCGGCGGTCACAACATATCATCGCCCTCTCACCTGGTATGCGGCAAGGGGTGATCGATGCTGG
GGTAGCTCCGGAAAAGATTAGTGTTATTCCGAACGCTGCCGATCTTGATCTCTTTCATCCCCAGCGTGGTGGCCGCCACT
GGCGGCAGCAGCTTGGCAATCCACCGTTCCTGGTGCTCTACTTCGGCACGATGGGTGAAGCTAACGACTTAAGCCAGGTG
ATAGAAGCGGCACGGGTTTTGCAACACCAGGGACGTAACGATATTCTGATCGTACTGGCCGGTCAGGGACGTCAGCGCTC
GTGGTTGGAAGAACAGAGTCGCCATTACGGTCTGCGTAATGTGCGCTTCCTTGATCCACTCTCCAAAGCGGAGGTAGCTG
ATCTGGTAGCGGCGGCTGATGTCTGTCTGACTATCTTCAAAGCTATTTCGGTACTGGCAACCTGTTCACCCAACAAACTG
TTCGACTCACTGGCAGCAGGGAAACCGGTCATTGTCAATACACCGGGGTGGCTGCAACACCTCGTCACCGAACATCAATG
CGGTCGCTATGCACGTGCCGGTGATCCCGCCGATCTGGCGGCGCAGATAGTGTATATGTGCGACCATCCAGAGTTTACCC
GCCAGGCCGGGCGGAATGCCCGTCTGCTGGCCGAACAGCGCTTTGATCGTCGCCGGTTGGCGTCCGAGGTGTTGGCCGTG
TTAGGGGCAGCAGTCTATCAAGAGCTACGCGTTTAG

Upstream 100 bases:

>100_bases
GGGCCGACGATGCACATCGACTGGGAACTGGTTGAACGCCTGCCACGAACCCGTGCCGGTAAATTGCGGCACGTCGTGCG
CGAGATCGAGGTGTAGTACG

Downstream 100 bases:

>100_bases
CGCCTGTTGCACGGCAACTTCGAGGGTGCTGGCGCGAAATGGCTTCTCAATTACTGCCAGATTGCTCATGTCGGTGCCTT
GCAATGCTGCAAAACCACTC

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MHILYLHQYFTTRTGVGGTRSYEFARFFIQQGHRVTMVTAANRQEPWSGGLWRRRVVDGINVVEVRAGYADYSRGTAISY
GQRIIAFLLFALASLIAVVRVERPDVVFATSTPLTIGIPGMLASRWHRVPLVFEVRDLWPEAPIQMGVLRHPLLILAARW
LERAIYRRSQHIIALSPGMRQGVIDAGVAPEKISVIPNAADLDLFHPQRGGRHWRQQLGNPPFLVLYFGTMGEANDLSQV
IEAARVLQHQGRNDILIVLAGQGRQRSWLEEQSRHYGLRNVRFLDPLSKAEVADLVAAADVCLTIFKAISVLATCSPNKL
FDSLAAGKPVIVNTPGWLQHLVTEHQCGRYARAGDPADLAAQIVYMCDHPEFTRQAGRNARLLAEQRFDRRRLASEVLAV
LGAAVYQELRV

Sequences:

>Translated_411_residues
MHILYLHQYFTTRTGVGGTRSYEFARFFIQQGHRVTMVTAANRQEPWSGGLWRRRVVDGINVVEVRAGYADYSRGTAISY
GQRIIAFLLFALASLIAVVRVERPDVVFATSTPLTIGIPGMLASRWHRVPLVFEVRDLWPEAPIQMGVLRHPLLILAARW
LERAIYRRSQHIIALSPGMRQGVIDAGVAPEKISVIPNAADLDLFHPQRGGRHWRQQLGNPPFLVLYFGTMGEANDLSQV
IEAARVLQHQGRNDILIVLAGQGRQRSWLEEQSRHYGLRNVRFLDPLSKAEVADLVAAADVCLTIFKAISVLATCSPNKL
FDSLAAGKPVIVNTPGWLQHLVTEHQCGRYARAGDPADLAAQIVYMCDHPEFTRQAGRNARLLAEQRFDRRRLASEVLAV
LGAAVYQELRV
>Mature_411_residues
MHILYLHQYFTTRTGVGGTRSYEFARFFIQQGHRVTMVTAANRQEPWSGGLWRRRVVDGINVVEVRAGYADYSRGTAISY
GQRIIAFLLFALASLIAVVRVERPDVVFATSTPLTIGIPGMLASRWHRVPLVFEVRDLWPEAPIQMGVLRHPLLILAARW
LERAIYRRSQHIIALSPGMRQGVIDAGVAPEKISVIPNAADLDLFHPQRGGRHWRQQLGNPPFLVLYFGTMGEANDLSQV
IEAARVLQHQGRNDILIVLAGQGRQRSWLEEQSRHYGLRNVRFLDPLSKAEVADLVAAADVCLTIFKAISVLATCSPNKL
FDSLAAGKPVIVNTPGWLQHLVTEHQCGRYARAGDPADLAAQIVYMCDHPEFTRQAGRNARLLAEQRFDRRRLASEVLAV
LGAAVYQELRV

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=351, Percent_Identity=24.7863247863248, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46033; Mature: 46033

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILYLHQYFTTRTGVGGTRSYEFARFFIQQGHRVTMVTAANRQEPWSGGLWRRRVVDGI
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHCCC
NVVEVRAGYADYSRGTAISYGQRIIAFLLFALASLIAVVRVERPDVVFATSTPLTIGIPG
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCH
MLASRWHRVPLVFEVRDLWPEAPIQMGVLRHPLLILAARWLERAIYRRSQHIIALSPGMR
HHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
QGVIDAGVAPEKISVIPNAADLDLFHPQRGGRHWRQQLGNPPFLVLYFGTMGEANDLSQV
CCCHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHH
IEAARVLQHQGRNDILIVLAGQGRQRSWLEEQSRHYGLRNVRFLDPLSKAEVADLVAAAD
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
VCLTIFKAISVLATCSPNKLFDSLAAGKPVIVNTPGWLQHLVTEHQCGRYARAGDPADLA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHH
AQIVYMCDHPEFTRQAGRNARLLAEQRFDRRRLASEVLAVLGAAVYQELRV
HHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHILYLHQYFTTRTGVGGTRSYEFARFFIQQGHRVTMVTAANRQEPWSGGLWRRRVVDGI
CEEEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHCCC
NVVEVRAGYADYSRGTAISYGQRIIAFLLFALASLIAVVRVERPDVVFATSTPLTIGIPG
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCH
MLASRWHRVPLVFEVRDLWPEAPIQMGVLRHPLLILAARWLERAIYRRSQHIIALSPGMR
HHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
QGVIDAGVAPEKISVIPNAADLDLFHPQRGGRHWRQQLGNPPFLVLYFGTMGEANDLSQV
CCCHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHH
IEAARVLQHQGRNDILIVLAGQGRQRSWLEEQSRHYGLRNVRFLDPLSKAEVADLVAAAD
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
VCLTIFKAISVLATCSPNKLFDSLAAGKPVIVNTPGWLQHLVTEHQCGRYARAGDPADLA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHHH
AQIVYMCDHPEFTRQAGRNARLLAEQRFDRRRLASEVLAVLGAAVYQELRV
HHHHHEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]