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The map label for this gene is nodB [H]

Identifier: 222525528

GI number: 222525528

Start: 2881686

End: 2882417

Strand: Direct

Name: nodB [H]

Synonym: Chy400_2277

Alternate gene names: 222525528

Gene position: 2881686-2882417 (Clockwise)

Preceding gene: 222525527

Following gene: 222525529

Centisome position: 54.69

GC content: 55.87

Gene sequence:

>732_bases
GTGATTCCTCAATCTTTTGTCACATACAGGTCTGGCCGGCATGTCAGACGCTGGTTCGCTCCAATCGGTCGGCGTCTCTT
CGGTACGCTGGTGCGGGTAGAGACGACTGAACCGCTGGTCGCGTTAACCTTCGACGATGGGCCACATCCTGTCTTTACGC
CGCAGATTCTCGATCTGTGTGAACAGTATCAGCTTCGGGCAACCTTTTTTCTGCTCGGCCAACATGCTGTCGATCAGCGT
GATCTGGTGACACGAATGGTAGCCGCCGGTCATGCGATTGGGAACCATACCTTCAGTCACATTCGTATGCCACAAACCCG
GCGCTGGCAACGCTGGCGTGAATTGTGGCAGACGCAGCAGGTACTCGCACCATACCGGCTGCGTTTATTTCGTCCTCCCT
ATGGCGGGCAATCTTACGGTTCACGCTTCGATGCCCTCCTGTTTGGGTATGAAGTTGTTGGCTGGACATTTCATATTGAA
GACTGGATTGCACAACCGGCCTCGCAACTGGCTGCACGGCTGGTAGATCAGGTGCGACCAGGGAGTATTATCTTGCTCCA
TGACCGCCTGGCAAATCCACGTGATCCCCAGGCAGCCGACCGTACCAGTCTGGTGTCGGCACTGGCAATGGCCTTACCCG
AGTTGCAACGTACCTATCGTTTTGTCACAGTACCTGAGTTGATCCGTGCCGGAACACCGGTGCGGGTGCCCTGGTTTCGT
CCGGCGTTGTAA

Upstream 100 bases:

>100_bases
CGGTTTGTTGCAGGGCAGTCTGCAATACCGGTGCCCGCCGCTGGCGATTTAGGTGTGAGAATCGTTTCGCTCCTCCCTGG
TCAGTGCTTGAAAGGTTACT

Downstream 100 bases:

>100_bases
TGATTGTGAGGCGCGACCATTGTGGACGCGCCGATTGGTGTTGTCACGTTAAGGAATCTTATGCCCAATGACAATCTTCT
CATTTCACCGTATGGTGGTC

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MIPQSFVTYRSGRHVRRWFAPIGRRLFGTLVRVETTEPLVALTFDDGPHPVFTPQILDLCEQYQLRATFFLLGQHAVDQR
DLVTRMVAAGHAIGNHTFSHIRMPQTRRWQRWRELWQTQQVLAPYRLRLFRPPYGGQSYGSRFDALLFGYEVVGWTFHIE
DWIAQPASQLAARLVDQVRPGSIILLHDRLANPRDPQAADRTSLVSALAMALPELQRTYRFVTVPELIRAGTPVRVPWFR
PAL

Sequences:

>Translated_243_residues
MIPQSFVTYRSGRHVRRWFAPIGRRLFGTLVRVETTEPLVALTFDDGPHPVFTPQILDLCEQYQLRATFFLLGQHAVDQR
DLVTRMVAAGHAIGNHTFSHIRMPQTRRWQRWRELWQTQQVLAPYRLRLFRPPYGGQSYGSRFDALLFGYEVVGWTFHIE
DWIAQPASQLAARLVDQVRPGSIILLHDRLANPRDPQAADRTSLVSALAMALPELQRTYRFVTVPELIRAGTPVRVPWFR
PAL
>Mature_243_residues
MIPQSFVTYRSGRHVRRWFAPIGRRLFGTLVRVETTEPLVALTFDDGPHPVFTPQILDLCEQYQLRATFFLLGQHAVDQR
DLVTRMVAAGHAIGNHTFSHIRMPQTRRWQRWRELWQTQQVLAPYRLRLFRPPYGGQSYGSRFDALLFGYEVVGWTFHIE
DWIAQPASQLAARLVDQVRPGSIILLHDRLANPRDPQAADRTSLVSALAMALPELQRTYRFVTVPELIRAGTPVRVPWFR
PAL

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323339, Length=131, Percent_Identity=32.824427480916, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323338, Length=128, Percent_Identity=32.03125, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 28093; Mature: 28093

Theoretical pI: Translated: 11.23; Mature: 11.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQSFVTYRSGRHVRRWFAPIGRRLFGTLVRVETTEPLVALTFDDGPHPVFTPQILDLC
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCHHHHHHH
EQYQLRATFFLLGQHAVDQRDLVTRMVAAGHAIGNHTFSHIRMPQTRRWQRWRELWQTQQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHH
VLAPYRLRLFRPPYGGQSYGSRFDALLFGYEVVGWTFHIEDWIAQPASQLAARLVDQVRP
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHCHHHHHHHHHHHHCCC
GSIILLHDRLANPRDPQAADRTSLVSALAMALPELQRTYRFVTVPELIRAGTPVRVPWFR
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCEECCCCC
PAL
CCC
>Mature Secondary Structure
MIPQSFVTYRSGRHVRRWFAPIGRRLFGTLVRVETTEPLVALTFDDGPHPVFTPQILDLC
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCHHHHHHH
EQYQLRATFFLLGQHAVDQRDLVTRMVAAGHAIGNHTFSHIRMPQTRRWQRWRELWQTQQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHH
VLAPYRLRLFRPPYGGQSYGSRFDALLFGYEVVGWTFHIEDWIAQPASQLAARLVDQVRP
HHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEHHHHHHCHHHHHHHHHHHHCCC
GSIILLHDRLANPRDPQAADRTSLVSALAMALPELQRTYRFVTVPELIRAGTPVRVPWFR
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCEECCCCC
PAL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968; 8850088 [H]