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The map label for this gene is dapE [H]

Identifier: 222525501

GI number: 222525501

Start: 2847883

End: 2849274

Strand: Direct

Name: dapE [H]

Synonym: Chy400_2249

Alternate gene names: 222525501

Gene position: 2847883-2849274 (Clockwise)

Preceding gene: 222525500

Following gene: 222525507

Centisome position: 54.05

GC content: 57.04

Gene sequence:

>1392_bases
ATGACCCCGGTGGCCGCTGCATTAAGTTATCTTACGGAACATCGTGATCAACATCTGTCTGCACTGTACGAATTGCTCAG
CATCCCCAGTGTAAGCATGGATCCGGCCCACCGGGCCGATATGGTGCGAGCCGCTGAATGGCTGGCAACCTGCCTGCGGC
ACATTGGGATGCACCACACCGAAATTGTCGCCGACCACGGGCTGCCACTGGTCTACAGCGAATGGCTTGGTGCCGAACAG
GCGCCAACGCTGCTCATCTACGGCCATTACGATGTACAGCCTGCCGACCCGGTAGCTGAATGGCATACACCACCCTTTAC
ACCCTCGCTGGTCGGTGAGAACCTCTATGCTCGCGGCGCATCCGATGACAAGGGCCAGGTGATGGCCGTGATCTTTGCCC
TGGCTTCCTGGCTCCAGACCAGCGGTCGTTTGCCGGTTAACGTTAAATTGATCATCGAGGGCGAAGAAGAAGTTTCGAGT
ACGCTGCTTCATCGTTTCGTGGCCGATCAGGCAGAACGGCTGCGTTGTGATGCGGTGTTGCTGGTGGATTCGATGATGCT
CAGCCCACAACAACCACTCATCCTCTACGGCACAAGGGGCAATTGTTATCTGGAAGTGACTGTGCGTGGCCCAAAGACCG
ATCTCCACTCCGGTACCTTTGGCGGTGTGGTGGATAATCCGTTCAACGTGCTGGTGCGCCTGCTTGCCGCACTGCAAGAT
GGTGATACACGCCGGGTTCGGGTACCGGGGTTTTACGAGAAAGTGCGCATCGTTGATGACGAAGAGCGAGCGTTTATCAA
TCGCCTACCGGTGAGTGATGCGGTATTGCAGGCGCTGACCGGTGTGCCGGCGCTGGCCGGAGAAGAGGGCTTTACACCGC
TTGAGCGGGCGAGTATCAGGCCAACCTTTGATATCCACGGCATCAGTGGCGGTTTCACGGGGCCGGGCAAAAAGACGGTG
ATCCCGGCTCAGGCCAGGGCCAAACTCTCGATGCGTCTGGTACCACATCAAGACCCGGTTGAAATTGGTGAACTGGTGCG
GAACTTCTTACAGCAGCAGGCACCATCAACCGTGCAGGTATCGGTAGAGGTATTGAGTGCATCGTGGCCGGTGCTGGTTG
ATTATCGTCATCCGACGGTGCAGGTGGCTTCAACAGCCTTCGCAAAGGCATTTGGGCGACCGGCGGCCTTCTCAATTGGC
GGAGGCACGCTGCCAATTGCGGCAACCTTCCAGAAACACCTGCAAGCGCCGCTCGTCATTACCGGCTTTGGCCTGCCTGA
CGACAACCTGCACGCGCCGAATGAAAAGATATTTCTGCCCTGCCTGTTTGGCGGCAGTGCAATGATCATTCACTATCTGG
CCGAACTGGCAGATGCGTATGTCTTAAGATAA

Upstream 100 bases:

>100_bases
ACAACGCAGCAGTGCCGTCAGCGGTCTGCCGTGGATTGTAGAGTAGAATAACCGGTAAGCGATTCTGCTTTCAAGCCAGG
TATTCATCAAGGAGGATGCG

Downstream 100 bases:

>100_bases
CCAGGTTGCAACCTTTATCACCACAGAGACACAGAGGAGATGGGGATAACCCAGGTTGATACTGTGAGGCGTGACAGGGT
GCTTGAACGTCTATTATCAC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTPVAAALSYLTEHRDQHLSALYELLSIPSVSMDPAHRADMVRAAEWLATCLRHIGMHHTEIVADHGLPLVYSEWLGAEQ
APTLLIYGHYDVQPADPVAEWHTPPFTPSLVGENLYARGASDDKGQVMAVIFALASWLQTSGRLPVNVKLIIEGEEEVSS
TLLHRFVADQAERLRCDAVLLVDSMMLSPQQPLILYGTRGNCYLEVTVRGPKTDLHSGTFGGVVDNPFNVLVRLLAALQD
GDTRRVRVPGFYEKVRIVDDEERAFINRLPVSDAVLQALTGVPALAGEEGFTPLERASIRPTFDIHGISGGFTGPGKKTV
IPAQARAKLSMRLVPHQDPVEIGELVRNFLQQQAPSTVQVSVEVLSASWPVLVDYRHPTVQVASTAFAKAFGRPAAFSIG
GGTLPIAATFQKHLQAPLVITGFGLPDDNLHAPNEKIFLPCLFGGSAMIIHYLAELADAYVLR

Sequences:

>Translated_463_residues
MTPVAAALSYLTEHRDQHLSALYELLSIPSVSMDPAHRADMVRAAEWLATCLRHIGMHHTEIVADHGLPLVYSEWLGAEQ
APTLLIYGHYDVQPADPVAEWHTPPFTPSLVGENLYARGASDDKGQVMAVIFALASWLQTSGRLPVNVKLIIEGEEEVSS
TLLHRFVADQAERLRCDAVLLVDSMMLSPQQPLILYGTRGNCYLEVTVRGPKTDLHSGTFGGVVDNPFNVLVRLLAALQD
GDTRRVRVPGFYEKVRIVDDEERAFINRLPVSDAVLQALTGVPALAGEEGFTPLERASIRPTFDIHGISGGFTGPGKKTV
IPAQARAKLSMRLVPHQDPVEIGELVRNFLQQQAPSTVQVSVEVLSASWPVLVDYRHPTVQVASTAFAKAFGRPAAFSIG
GGTLPIAATFQKHLQAPLVITGFGLPDDNLHAPNEKIFLPCLFGGSAMIIHYLAELADAYVLR
>Mature_462_residues
TPVAAALSYLTEHRDQHLSALYELLSIPSVSMDPAHRADMVRAAEWLATCLRHIGMHHTEIVADHGLPLVYSEWLGAEQA
PTLLIYGHYDVQPADPVAEWHTPPFTPSLVGENLYARGASDDKGQVMAVIFALASWLQTSGRLPVNVKLIIEGEEEVSST
LLHRFVADQAERLRCDAVLLVDSMMLSPQQPLILYGTRGNCYLEVTVRGPKTDLHSGTFGGVVDNPFNVLVRLLAALQDG
DTRRVRVPGFYEKVRIVDDEERAFINRLPVSDAVLQALTGVPALAGEEGFTPLERASIRPTFDIHGISGGFTGPGKKTVI
PAQARAKLSMRLVPHQDPVEIGELVRNFLQQQAPSTVQVSVEVLSASWPVLVDYRHPTVQVASTAFAKAFGRPAAFSIGG
GTLPIAATFQKHLQAPLVITGFGLPDDNLHAPNEKIFLPCLFGGSAMIIHYLAELADAYVLR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=475, Percent_Identity=30.5263157894737, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI21071039, Length=467, Percent_Identity=31.2633832976445, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI271398277, Length=321, Percent_Identity=29.9065420560748, Blast_Score=150, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI193211059, Length=477, Percent_Identity=32.0754716981132, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17563146, Length=462, Percent_Identity=32.2510822510823, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321159, Length=467, Percent_Identity=29.7644539614561, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319758, Length=453, Percent_Identity=26.0485651214128, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24585880, Length=470, Percent_Identity=33.8297872340426, Blast_Score=231, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50444; Mature: 50313

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVAAALSYLTEHRDQHLSALYELLSIPSVSMDPAHRADMVRAAEWLATCLRHIGMHHT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EIVADHGLPLVYSEWLGAEQAPTLLIYGHYDVQPADPVAEWHTPPFTPSLVGENLYARGA
EEHHCCCCCEEEHHHCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCEECCC
SDDKGQVMAVIFALASWLQTSGRLPVNVKLIIEGEEEVSSTLLHRFVADQAERLRCDAVL
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDSMMLSPQQPLILYGTRGNCYLEVTVRGPKTDLHSGTFGGVVDNPFNVLVRLLAALQD
HHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GDTRRVRVPGFYEKVRIVDDEERAFINRLPVSDAVLQALTGVPALAGEEGFTPLERASIR
CCCCEEECCCHHHEEEEECCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
PTFDIHGISGGFTGPGKKTVIPAQARAKLSMRLVPHQDPVEIGELVRNFLQQQAPSTVQV
CCEEEECCCCCCCCCCCCEEECCHHHCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
SVEVLSASWPVLVDYRHPTVQVASTAFAKAFGRPAAFSIGGGTLPIAATFQKHLQAPLVI
EEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEE
TGFGLPDDNLHAPNEKIFLPCLFGGSAMIIHYLAELADAYVLR
EECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPVAAALSYLTEHRDQHLSALYELLSIPSVSMDPAHRADMVRAAEWLATCLRHIGMHHT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EIVADHGLPLVYSEWLGAEQAPTLLIYGHYDVQPADPVAEWHTPPFTPSLVGENLYARGA
EEHHCCCCCEEEHHHCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHCCCCEECCC
SDDKGQVMAVIFALASWLQTSGRLPVNVKLIIEGEEEVSSTLLHRFVADQAERLRCDAVL
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LVDSMMLSPQQPLILYGTRGNCYLEVTVRGPKTDLHSGTFGGVVDNPFNVLVRLLAALQD
HHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GDTRRVRVPGFYEKVRIVDDEERAFINRLPVSDAVLQALTGVPALAGEEGFTPLERASIR
CCCCEEECCCHHHEEEEECCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCC
PTFDIHGISGGFTGPGKKTVIPAQARAKLSMRLVPHQDPVEIGELVRNFLQQQAPSTVQV
CCEEEECCCCCCCCCCCCEEECCHHHCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
SVEVLSASWPVLVDYRHPTVQVASTAFAKAFGRPAAFSIGGGTLPIAATFQKHLQAPLVI
EEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCEEE
TGFGLPDDNLHAPNEKIFLPCLFGGSAMIIHYLAELADAYVLR
EECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA